53BP2 Antibody - BSA Free

Western Blot: 53BP2 Antibody [NBP1-77907] - Detection of over-expressed ASPP2 in MCF-7 cells (Lane 2, arrowhead). Lane 1 is a nontransfected control. Lane 3 is MCF-7 cells over-expressing ASPP1. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. The identity of the lower MW band at approximately 40kDa is unknown.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

53BP2 Antibody - BSA Free Summary

• Description: This Protein A purified antibody is directed against human 53BP2. The product was purified from monospecific antiserum by Protein A affinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified 53BP2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human 53BP2. (Uniprot: Q13625)
• Specificity: This Protein A purified antibody is directed against human 53BP2. Minimal reactivity occurs against ASPP1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TP53BP2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2500-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:3000
• Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 125 kDa in size corresponding to ASPP2 by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with 53BP2 from macaque and dog sources based on a 100% homology with the immunizing sequence. Expect partial reactivity with 53BP2 from mouse and rat sources based on an 80% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for 53BP2 Antibody - BSA Free

• apoptosis-stimulating protein of p53, 2
• ASPP2P53BP2,53BP2apoptosis-stimulating of p53 protein 2
• Bbp
• Bcl2-binding protein
• p53-binding protein 2
• p53BP2
• PPP1R13A
• Renal carcinoma antigen NY-REN-51
• tumor protein p53 binding protein, 2
• tumor protein p53-binding protein, 2
• Tumor suppressor p53-binding protein 2

Background

ASPP (ankyrin-repeat-, SH3-domain- proline-rich-region protein) proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, iASPP functionally inactivates p53. ASPPs may also regulate p63- and p73-mediated apoptosis. Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3. Not all cell cycle arrest genes are affected, such as p21. Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TP53BP2
• Entrez:
  • 7159(Human)
• Uniprot:
  • Q13625(Human)