53BP2 Antibody

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Western Blot: 53BP2 Antibody [NBP1-77907] - Detection of over-expressed ASPP2 in MCF-7 cells (Lane 2, arrowhead). Lane 1 is a nontransfected control. Lane 3 is MCF-7 cells over-expressing ASPP1. Cell extracts were ...read more

Product Details

Summary
Reactivity Hu, Ca, MkSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2.4 mg/ml

Order Details

53BP2 Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human 53BP2.
Specificity
This Protein A purified antibody is directed against human ASPP2. Minimal reactivity occurs against ASPP1. A BLAST analysis was used to suggest cross-reactivity.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TP53BP2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • ELISA 1:2500-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Expect a band approximately 125 kDa in size corresponding - ASPP2 by western blotting in the appropriate cell lysate or extract.
Control
53BP2 Lysate (NBP2-65091)

Reactivity Notes

mouse and rat sources based on an 80% homology

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
2.4 mg/ml
Purity
Protein A purified

Alternate Names for 53BP2 Antibody

  • apoptosis-stimulating protein of p53, 2
  • ASPP2P53BP2,53BP2apoptosis-stimulating of p53 protein 2
  • Bbp
  • Bcl2-binding protein
  • p53-binding protein 2
  • p53BP2
  • PPP1R13A
  • Renal carcinoma antigen NY-REN-51
  • tumor protein p53 binding protein, 2
  • tumor protein p53-binding protein, 2
  • Tumor suppressor p53-binding protein 2

Background

ASPP (ankyrin-repeat-, SH3-domain- proline-rich-region protein) proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, iASPP functionally inactivates p53. ASPPs may also regulate p63- and p73-mediated apoptosis. Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3. Not all cell cycle arrest genes are affected, such as p21. Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

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Bioinformatics

Gene Symbol TP53BP2
Entrez
Uniprot