5'-Nucleotidase/CD73 Antibody (JM11-40)

Western Blot: 5'-Nucleotidase/CD73 Antibody (JM11-40) [NBP2-67101] - Western blot analysis of 5'-Nucleotidase/CD73 on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (JM11-40) [NBP2-67101] - Analysis of paraffin-embedded human uterus tissue using anti-CD73 antibody. Counter stained with hematoxylin.

Western Blot: 5'-Nucleotidase/CD73 Antibody (JM11-40) [NBP2-67101] - Analysis of CD73 on A431 cells lysates using anti-CD73 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (JM11-40) [NBP2-67101] - Analysis of paraffin-embedded human breast cancer tissue using anti-CD73 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (JM11-40) [NBP2-67101] - Analysis of paraffin-embedded human tonsil tissue using anti-CD73 antibody. Counter stained with hematoxylin.

Product Discontinued

5'-Nucleotidase/CD73 Antibody (JM11-40) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human 5'-Nucleotidase/CD73 aa 491-536 / 574. (SwissProt: P21589 Human)
• Localization: Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NT5E
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for 5'-Nucleotidase/CD73 Antibody (JM11-40)

• 5' nucleotidase (CD73)
• 5'-NT
• 5-NT
• 5'-Nucleotidase
• 5'-nucleotidase, ecto (CD73)
• CD73 antigen
• CD73
• E5NT
• EC 3.1.3.5
• ecto-5'-nucleotidase
• eN
• eNT
• NT
• NT55'-nucleotidase
• NT5E
• NTE
• Purine 5-Prime-Nucleotidase

Background

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for 5'-Nucleotidase/CD73 Antibody (NBP2-67101). (Showing 1 - 1 of 1 FAQ).

1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: NT5E