5'-Nucleotidase/CD73 Antibody (45M3B4)

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Western Blot: 5'-Nucleotidase/CD73 Antibody (45M3B4) [NBP2-25235] - Analysis of CD73 in a partial recombinant protein using CD73 antibody at 1 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB
Clone
45M3B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

5'-Nucleotidase/CD73 Antibody (45M3B4) Summary

Immunogen
Within the range of amino acids 169-324 of human CD73 were used as the immunogen for the antibody.
Specificity
This product can detect Isoform 1 and Isoform of Human CD73.
Predicted Species
Mouse (94%), Rat (94%), Bovine (95%). Backed by our 100% Guarantee.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NT5E
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Publications
Read Publication using
NBP2-25235 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for 5'-Nucleotidase/CD73 Antibody (45M3B4)

  • 5' nucleotidase (CD73)
  • 5'-NT
  • 5-NT
  • 5'Nucleotidase
  • 5'-Nucleotidase
  • 5'-nucleotidase, ecto (CD73)
  • CD73 antigen
  • CD73
  • E5NT
  • EC 3.1.3.5
  • ecto-5'-nucleotidase
  • eN
  • eNT
  • NT
  • NT55'-nucleotidase
  • NT5E
  • NTE
  • Purine 5-Prime-Nucleotidase

Background

CD73 is a membrane-bound enzyme having a key role in extracellular ATP metabolism producing adenosine by dephosphorylating AMP. It is highly expressed on endothelial cells but a moderate expression is also observed on a subpopulation of lymphocytes, especially regulatory T cells. Although CD73 on the endothelial cells and lymphocytes are structurally similar, they are differently regulated. Triggering of lymphocyte CD73 with antibodies results in cleavage of CD73 and activation of LFA-1, leading to increased adhesion of lymphocytes to endothelium (Algars A et al., 2011). CD73 plays an important role in carcinogenesis, as adenosine promotes tumor progression and CD73-expressing cancer cell lines are more aggressive (Supernat A et al., 2012). Extracellular adenosine is a potent immunosuppressor that accumulates during tumor growth. Anti-CD73 monoclonal antibody therapy significantly delays primary 4T1.2 and E0771 tumor growth in immune-competent mice and significantly inhibits the development of spontaneous 4T1.2 lung metastases. Antibody therapy is essentially dependent on the induction of adaptive anti-tumor immune responses (Stagg J et al., 2010).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 5'-Nucleotidase/CD73 Antibody (NBP2-25235)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: FLOW.


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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for 5'-Nucleotidase/CD73 Antibody (NBP2-25235). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-25235AF405
Alexa Fluor 488 Labeled NBP2-25235AF488
Alexa Fluor 647 Labeled NBP2-25235AF647
Alexa Fluor 700 Labeled NBP2-25235AF700
Biotin Labeled NBP2-25235B
DyLight 350 Labeled NBP2-25235UV
DyLight 405 Labeled NBP2-25235V
DyLight 488 Labeled NBP2-25235G
DyLight 550 Labeled NBP2-25235R
DyLight 650 Labeled NBP2-25235C
DyLight 680 Labeled NBP2-25235FR
DyLight 755 Labeled NBP2-25235IR
HRP Labeled NBP2-25235H
PerCP Labeled NBP2-25235PCP

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Bioinformatics Tool for 5'-Nucleotidase/CD73 Antibody (NBP2-25235)

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Diseases for 5'-Nucleotidase/CD73 Antibody (NBP2-25235)

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Pathways for 5'-Nucleotidase/CD73 Antibody (NBP2-25235)

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PTMs for 5'-Nucleotidase/CD73 Antibody (NBP2-25235)

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Research Areas for 5'-Nucleotidase/CD73 Antibody (NBP2-25235)

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Blogs on 5'-Nucleotidase/CD73.

Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target
By Yoskaly Lazo-Fernandez, PhD Role of Adenosine in the Tumor Microenvironment a Target for Cancer TherapyThe tumor microenvironment (TME) tends to be concentrated in the purine nucleoside adenosine, a direct resu...  Read full blog post.

CD73 (Cluster of differentiation 73, ecto-5'-nucleotidase)
CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble...  Read full blog post.

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Bioinformatics

Gene Symbol NT5E
Entrez
Uniprot