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5'-Nucleotidase/CD73 Antibody (102) [DyLight 755]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, ICC/IF
Clone
102
Clonality
Monoclonal
Host
Rabbit
Conjugate
DyLight 755

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-89706AF350
Alexa Fluor 405
NBP2-89706AF405
Alexa Fluor 488
NBP2-89706AF488
Alexa Fluor 532
NBP2-89706AF532
Alexa Fluor 594
NBP2-89706AF594
Alexa Fluor 647
NBP2-89706AF647
Alexa Fluor 700
NBP2-89706AF700
Alexa Fluor 750
NBP2-89706AF750
Biotin
NBP2-89706B
DyLight 350
NBP2-89706UV
DyLight 405
NBP2-89706V
DyLight 488
NBP2-89706G
DyLight 550
NBP2-89706R
DyLight 594
NBP2-89706DL594
DyLight 650
NBP2-89706C
DyLight 680
NBP2-89706FR
FITC
NBP2-89706F
HRP
NBP2-89706H
Janelia Fluor 549
NBP2-89706JF549
Janelia Fluor 646
NBP2-89706JF646
Unconjugated
NBP2-89706-100ul
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5'-Nucleotidase/CD73 Antibody (102) [DyLight 755] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human 5'-Nucleotidase/CD73 (NP_002517.1; Met1-Lys547).
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NT5E
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for 5'-Nucleotidase/CD73 Antibody (102) [DyLight 755]

  • 5' nucleotidase (CD73)
  • 5'-NT
  • 5-NT
  • 5'-Nucleotidase
  • 5'-nucleotidase, ecto (CD73)
  • CD73 antigen
  • CD73
  • E5NT
  • EC 3.1.3.5
  • ecto-5'-nucleotidase
  • eN
  • eNT
  • NT
  • NT55'-nucleotidase
  • NT5E
  • NTE
  • Purine 5-Prime-Nucleotidase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: Neurotensin Antibody [NBP2-33903] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Immunohistochemistry-Paraffin: Neurotensin Antibody [NBP2-33903] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
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Deaminase/ADA Antibod
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Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Analysis in human duodenum and testis tissues using NBP1-87404 antibody. Corresponding Adenosine Deaminase/ADA RNA-seq data are presented for the same tissues.
Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
(2 Publications)
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CTLA-4
[Unconjugated]
7268-CT
CTLA-4 [Unconjugated]
(3 Reviews)
(1 Publication)
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Endoglin/CD105
Antibody (3A9)
NBP2-22122
Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody at 2 ug/ml and detected with an anti-mouse HRP labeled secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - HMSC cells stained with anti-CD105 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse mAb with DAB staining.
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Applications: WB, ELISA, ICC/IF, IHC, IHC-P
HLA DQ Antibody
(SPV-L3)
NBP2-45041
Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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BDNF [HRP]
DBD00
BDNF [HRP]
(4 Reviews)
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Species: Hu
NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
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Acetylcholinesterase/A
NBP1-31329
Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Paraformaldehyde-fixed HeLa, using AChE antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.
Immunohistochemistry-Frozen: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Frozen sectioned E13.5 Rat brain. Green: AChE protein stained by AChE antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.
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CD90/Thy1 Antibody
(OX-7)
NB100-65543
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CD45 Antibody
(30-F11)
NB100-77417
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Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - VP1-positive infiltrating M2 macrophage and VP1-positive pneumocytes were detectable in the orally infected lung. Adjacent lung sections were examined with H&E and IHC staining for a pan-leukocyte marker CD45. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY licence.
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PNPLA6 Antibody
(3D10)
H00010908-M08
Western Blot: PNPLA6 Antibody (3D10) [H00010908-M08] - Analysis of PNPLA6 expression in transfected 293T cell line by NTE monoclonal antibody (M08), clone 3D10. Lane 1: PNPLA6 transfected lysate (Predicted MW: 146.2 KDa). Lane 2: Non-transfected lysate.
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CD34 Antibody (MEC
14.7)
NB600-1071
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Antibody
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Immunocytochemistry/Immunofluorescence: Substance P Antibody [NB300-187] - Staining of trigeminal ganglia from P3-P5 rat pups. BMC Neuroscience 2010, 11:32doi:10.1186/1471-2202-11-32
Immunohistochemistry: Substance P Antibody [NB300-187] - Spinal Cord Dorsal Horn (Human).
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NT-3 [Unconjugated]
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Immunocytochemistry/Immunofluorescence: ADK Antibody [NBP2-15291] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
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CD44 Antibody
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Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of FFPE human breast carcinoma tissues using CD44 antibody (8E2F3). The signal was developed using DAB based detection and the sections were processed for counterstaining with hematoxylin. The antibody generated mainly a membrane staining representative of CD44 protein.
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Insulin Antibody
(E2-E3 (same as INS04 ...
NBP2-34260
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Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
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Publications for 5'-Nucleotidase/CD73 Antibody (NBP2-89706IR) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for 5'-Nucleotidase/CD73 Antibody (NBP2-89706IR). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-89706AF350
Alexa Fluor 405 NBP2-89706AF405
Alexa Fluor 488 NBP2-89706AF488
Alexa Fluor 532 NBP2-89706AF532
Alexa Fluor 594 NBP2-89706AF594
Alexa Fluor 647 NBP2-89706AF647
Alexa Fluor 700 NBP2-89706AF700
Alexa Fluor 750 NBP2-89706AF750
Biotin NBP2-89706B
DyLight 350 NBP2-89706UV
DyLight 405 NBP2-89706V
DyLight 488 NBP2-89706G
DyLight 550 NBP2-89706R
DyLight 594 NBP2-89706DL594
DyLight 650 NBP2-89706C
DyLight 680 NBP2-89706FR
DyLight 755 NBP2-89706IR
FITC NBP2-89706F
HRP NBP2-89706H
Janelia Fluor 549 NBP2-89706JF549
Janelia Fluor 646 NBP2-89706JF646
Learn about Custom Antibody Labeling

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Bioinformatics Tool for 5'-Nucleotidase/CD73 Antibody (NBP2-89706IR)

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Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target
By Yoskaly Lazo-Fernandez, PhD Role of Adenosine in the Tumor Microenvironment a Target for Cancer TherapyThe tumor microenvironment (TME) tends to be concentrated in the purine nucleoside adenosine, a direct resu...  Read full blog post.
CD73 (Cluster of differentiation 73, ecto-5'-nucleotidase)
CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble...  Read full blog post.
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Bioinformatics

Gene Symbol NT5E