5-HT4 Antibody

Product Discontinued

5-HT4 Antibody Summary

• Immunogen: Synthetic 17 amino acid peptide from C-terminus of human 5HT4 Receptor.
• Specificity: Human 5HT4 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Chicken (94%), Primate (94%), Turkey (94%), Monkey (100%), Opossum (94%), Guinea Pig (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HTR4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 20836992). Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (94%), Mouse (88%), Porcine (82%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 5-HT4 Antibody

• 5HT4
• 5-HT4
• 5-HT-4
• 5-HT4R
• 5-hydroxytryptamine (serotonin) receptor 4,5-hydroxytryptamine receptor 4,5-HT45-hydroxytryptamine4 receptor
• cardiac 5-HT4 receptor
• HTR4
• Serotonin receptor 4

Background

The 5-HT4 receptor, a member of the Serotonin Receptor subfamily, has been shown to affect the sensory and motor functions of the gut. Some of the most promising emerging therapies in Irritable Bowel Syndrome revolve around targeted pharmacotherapeutic modulation of serotonin receptors (ie, 5-HT3 and 5-HT4 subtypes). At least 9 isoforms (5-HT4A-5-HT4G, and 5-HT4n) have been described, which differ in the length and composition of their intracellular C termini after a common splicing site (L358). A ninth isoform (5-HT4H) results in the insertion of 14 amino acids into the second intracellular loop. 5-HT4 receptor expression has been documented primarily in the brain, and in colon, heart, and vessel. An EST has been isolated from a human cancerous nerve library.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 5-HT4 Antibody (NLS656)(4)

Publications using NLS656

• Ennes H S, Mayer E A, Hicks G A et al. Expression of 5-Ht4receptor Splice Variants by Drg Neurons.
• Nedi T, White PJ, Coupar IM, Irving HR. Tissue dependent differences in G-protein coupled receptor kinases associated with 5-HT4 receptor desensitization in the rat gastro-intestinal tract. Biochem Pharmacol 2011-01-01 [PMID: 20836992] (Rat)
• [No authors listed]. The American Gastroenterlogical Association (AGA) Institute and Digestive Disease Week, May 17-22, 2008 San Diego, CA, USA. Abstracts. Gastroenterology. [PMID: 20131410]
• Fang X, Liu S, Wang XY et al. Neurogastroenterology of tegaserod (HTF 919) in the submucosal division of the guinea-pig and human enteric nervous system. Neurogastroenterol Motil 2008-01-01 [PMID: 17973634]

Bioinformatics

• Gene Symbol: HTR4
• Entrez:
  • 3360(Human)
  • 15562(Mouse)
  • 25324(Rat)
• Uniprot:
  • Q13639(Human)