RPS5 Antibody (4H3)

Images

 
Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - Analysis of RPS5 expression in transfected 293T cell line by RPS5 monoclonal antibody (M02), clone 4H3.Lane 1: RPS5 transfected lysate(22.9 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: RPS5 Antibody (4H3) [H00006193-M02] - Analysis of monoclonal antibody to RPS5 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - RPS5 monoclonal antibody (M02), clone 4H3 Analysis of RPS5 expression in HeLa.
Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - RPS5 monoclonal antibody (M02), clone 4H3. Analysis of RPS5 expression in PC-12.
Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - RPS5 monoclonal antibody (M02), clone 4H3. Analysis of RPS5 expression in Raw 264.7.
Western Blot: RPS5 Antibody (4H3) [H00006193-M02] - RPS5 monoclonal antibody (M02), clone 4H3. Analysis of RPS5 expression in NIH/3T3.
ELISA: RPS5 Antibody (4H3) [H00006193-M02] - Detection limit for recombinant GST tagged RPS5 is approximately 3ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, PrSpecies Glossary
Applications WB, ELISA, ICC/IF, S-ELISA
Clone
4H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RPS5 Antibody (4H3) Summary

Immunogen
RPS5 (NP_001000, 106 a.a. ~ 204 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ENPLQVLVNAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR
Specificity
RPS5 (4H3)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RPS5
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Sandwich ELISA 1:100-1:2000
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Publications
Read Publications using
H00006193-M02 in the following applications:

  • WB
    2 publications

Reactivity Notes

Trypanosoma brucei reactivity reported in scientific literature (PMID: 24706018). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RPS5 Antibody (4H3)

  • ribosomal protein S5,40S ribosomal protein S5

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S7P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RPS5 Antibody (H00006193-M02)(2)

We have publications tested in 1 confirmed species: Trypanosoma brucei.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Filter By Species
Trypanosoma brucei
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RPS5 Antibody (H00006193-M02) (0)

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Bioinformatics

Gene Symbol RPS5
Entrez
OMIM
Uniprot