4-1BB/TNFRSF9/CD137 Antibody [Biotin]

• Applications: WB, Flow, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

4-1BB/TNFRSF9/CD137 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse 4‑1BB/TNFRSF9/CD137
  Val24-Leu187
  Accession # P20334
• Specificity: Detects mouse 4‑1BB/TNFRSF9/CD137 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity with recombinant human 4-1BB, recombinant mouse (rm) EDAR, and rmCD40 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFRSF9
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Application Notes: ELISA Capture: Mouse 4-1BB/TNFRSF9/CD137 Antibody (Catalog # MAB937)
  ELISA Detection: Mouse 4-1BB/TNFRSF9/CD137 Biotinylated Antibody (Catalog # BAF937)
  Standard: Recombinant Mouse 4-1BB/TNFRSF9/CD137 Fc Chimera (Catalog # 937-4B)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

4-1BB, also known as CD137 and ILA (induced by lymphocyte activation), is a TNF receptor superfamily member and has been designated TNFRSF9. Mouse 4‑1BB cDNA encodes a 256 amino acid (aa) residues type I transmembrane protein with a putative 23 aa signal peptide, a 164 aa extracellular domain, a 21 aa transmembrane domain and a 48 aa cytoplasmic region (1‑3). A soluble 4‑1BB is released from surfaces of cells expressing the transmembrane protein (4). Mouse 4‑1BB shares approximately 60% aa sequence identity with its human counterpart. 4‑1BB is expressed on activated CD4⁺ and CD8⁺ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, DCs and eosinophils (5). The ligand for 4‑1BB (4‑1BBL), also named TNFSF9, belongs to the TNF ligand superfamily. 4‑1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines. In response to 4‑1BBL binding, 4‑1BB transduce a T cell costimulatory signal in both CD4⁺ and CD8⁺ T cells to promote survival and enhance proliferation, cytokine production and effector function. In vivo, the costimulatory activity of 4‑1BB has been shown to be important in graft-vs-host disease and antiviral CTL responses. On dendritic cells, 4‑1BB is a DC-activating molecules that enhances cytokine production and up‑regulates expression of B7-1 and B7-2 costimulatory molecules, resulting in an improved ability to stimulate T cell responses (1‑5).

1. Goodwin, R.G. et al. (1993) Eur. J. Immunol. 23:2631.
2. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
3. Kwon, B.S. and S.M. Weissman (1989) Proc. Nat. Acad. Sci. USA 86:1963.
4. Wilcox, R.A. et al. (2002) J. Immunol. 168:4262.
5. Kwon, B., H.W. Lee and B.S. Kwon, 2002, TRENDS in Immunology 23:378.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF9