OX40/TNFRSF4 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human T regulatory cells (Tregs) and human peripheral blood mononuclear cells (PBMCs) untreated (-) or treated (+) with 1 μg/mL PHA for 5 days. PVDF membrane was probed with 0.5 ...read more
Human T cells were treated for 3‑5 days with 1 μg/mL PHA then stained with Sheep Anti-Human OX40/TNFRSF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3388, filled histogram) or control antibody (Catalog ...read more
OX40/TNFRSF4 was detected in immersion fixed paraffin-embedded sections of human tonsil using Sheep Anti-Human OX40/TNFRSF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3388) at 3 µg/mL for 1 hour ...read more
Recombinant Human OX40L/TNFSF4 (Catalog # 1054-OX) stimulates CXCL8/IL-8 secretion in the HT1080 human fibrosarcoma cell line transfected with human OX40/TNFRSF4 in a dose-dependent manner (orange line), as measured ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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OX40/TNFRSF4 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human OX40
Leu29-Ala216
Accession # P43489
Specificity
Detects human OX40/TNFRSF4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse OX40 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
TNFRSF4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 3-15 ug/mL
  • Neutralization 0.025-0.16 ug/mL
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF3388 in the following applications:

Publications
Read Publications using
AF3388 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OX40/TNFRSF4 Antibody [Unconjugated]

  • ACT-135
  • ACT35 antigen
  • ACT35ATC35 antigen
  • CD134 antigen
  • CD134
  • Ly-70
  • OX40 cell surface antigen
  • OX40 homologue
  • OX40
  • OX40L receptor
  • OX40lymphoid activation antigene ACT35
  • TAX transcriptionally-activated glycoprotein 1 receptor
  • tax-transcriptionally activated glycoprotein 1 receptor
  • TNFRSF4
  • tumor necrosis factor receptor superfamily member 4
  • tumor necrosis factor receptor superfamily, member 4
  • Txgp1
  • TXGP1L
  • TXGP1LOX40 antigen

Background

OX40 (CD134; TNFRSF4) is a T cell costimulatory molecule of the TNF receptor superfamily that coordinates with other membrane-bound costimulators such as CD28, CD40, CD30, CD27 and 4-1BB (1‑3). OX40 is expressed on naïve CD4+ T cells only after engagement of the TCR by antigen presenting cells (APC; dendritic and B cells), and costimulation by CD40/CD40 ligand and CD28/B7. It is maximal at 2‑5 days post activation, or 4 hours post reactivation of memory T cells (3‑6). Human OX40 is a 48 kDa type I transmembrane glycoprotein with a 28 amino acid (aa) signal sequence, a 185 aa extracellular domain (ECD) that has four TNFR-Cys repeats and an O-glycosylated hinge region, a 20 aa transmembrane segment, and a 41 aa cytoplasmic domain (3). The ECD of human OX40 shows 71%, 68%, 67%, 64% and 64% aa identity with feline, canine, rabbit, mouse and rat OX40 ECD, respectively. Engagement of OX40 on activated CD4+ T cells by OX40 ligand on activated dendritic cells promotes T cell survival and proliferation, prolongs the immune response, and enhances the number of cells making the transition from effector to memory T cells (1‑6). OX40 signal transduction includes binding TNF receptor-associated factors (TRAFs), and activating NF kappa B and PI3 kinase to enhance expression of cytokines, antiapoptotic Bcl-2 family members, survivin and the chemokine receptor CXCR5 (5‑8). CXCR5 promotes T cell migration to germinal centers to deliver B cell help (5). Studies using knockout or transgenic mice, and agonistic or blocking antibodies, show that OX40/OX40L interaction is critical for establishing or reactivating memory T cells and breaking immune tolerance (9). Blockade of OX40 engagement is efficacious in animal models of allergic airway inflammation, graft-versus-host disease and autoimmune disease (10‑14).

 

  1. Salek-Ardakani, S. and M. Croft (2006) Vaccine 24:872.
  2. Hori, T. (2006) Int. J. Hematol. 83:17.
  3. Latza, U. et al. (1994) Eur. J. Immunol. 24:677.
  4. Murata, K. et al. (2000) J. Exp. Med. 191:365.
  5. Fillatreau, S and D. Gray (2003) J. Exp. Med. 197:195.
  6. Gramaglia, I. et al. (1998) J. Immunol. 161:6510.
  7. Rogers, P.R. et al. (2001) Immunity 15:445.
  8. Song, J. et al. (2005) Immunity 22:621.
  9. Bansal-Pakala, P. et al. (2001) Nat. Med. 7:907.
  10. Salek-Ardakani, S. et al. (2003) J. Exp. Med. 198:315.
  11. Jember, A. G. et al. (2001) J. Exp. Med. 193:387.
  12. Demirci, G. et al. (2004) J. Immunol. 172:1691.
  13. Blazar, B.R. et al. (2003) Blood 101:3741.
  14. Higgins, L.M. et al. (1999) J. Immunol. 162:486.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OX40/TNFRSF4 Antibody (AF3388)(2)

Review for OX40/TNFRSF4 Antibody (AF3388) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF3388:
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Immunofluorescence - paraffin
(1)
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Human
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  4
reviewed by:
Verified Customer
Immunofluorescence - paraffin Human 02/29/2020
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Summary

ApplicationOther
Sample Testedhuman tonsil
SpeciesHuman
LotN/A

Comments

CommentspH 9 antigen retrieval

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Video Protocols

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Verified Customer
02/29/2020
Application: Immunofluorescence - paraffin
Species: Human

Bioinformatics

Gene Symbol TNFRSF4
Uniprot