4-1BB/TNFRSF9/CD137 Antibody (158321)

• Reactivity: Mu
• Applications: Func
• Clone: 158321
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

4-1BB/TNFRSF9/CD137 Antibody (158321) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse 4-1BB
  Val24-Leu187
  Accession # P20334
• Specificity: Detects mouse 4-1BB in direct ELISAs and Western blots. In direct ELISAs, this antibody shows no cross-reactivity with recombinant mouse (rm) BAFF R, rmCD27, rmCD30, rmCD40, rmDR3, rmEDAR, rmFas, rmGITR, rmLT beta R, rmNGF R, rmOPG, rmRANK, rmTAJ, rmTNF RI, rmTNF RII, rh4-1BB, rhDR6, or rhHVEM.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TNFRSF9
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Agonist Activity

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 4-1BB/TNFRSF9/CD137 Antibody (158321)

• 4-1BB ligand receptor
• 41BB
• 4-1BB
• CD137 antigen
• CD137
• CD137MGC2172
• CDw137
• FLJ43501
• ILA
• ILAhomolog of mouse 4-1BB
• induced by lymphocyte activation (ILA)
• interleukin-activated receptor, homolog of mouse Ly63
• receptor protein 4-1BB
• T cell antigen ILA
• T-cell antigen 4-1BB homolog
• T-cell antigen ILA
• TNFRSF9
• tumor necrosis factor receptor superfamily member 9
• tumor necrosis factor receptor superfamily, member 9

Background

4-1BB is a type I transmembrane protein belonging to the TNF receptor superfamily. It has been designated TNFRSF9 and is also known as CD137. 4-1BB is expressed on T cells, NK cells, monocytes, neutrophils, dendritic cells, and eosinophils. The ligand for 4-1BB, 4-1BBL, is a TNF ligand superfamily member (TNFSF9) expressed on the surfaces of activated antigen presenting cells and T and B lymphoma cell lines. 4-1BB transduces a T cell costimulatory signal to promote survival, proliferation, cytokine production, and effector function. On dendritic cells, 4-1BB ligation enhances cytokine production and upregulates expression of B7-1 and B7-2 costimulatory molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 4-1BB/TNFRSF9/CD137 Antibody (MAB9371)(6)

Publications using MAB9371

• E Dodagatta-, HY Ma, B Liang, J Li, DS Meyer, SY Chen, KH Sun, X Ren, B Zivak, MD Rosenblum, MB Headley, L Pinzas, NI Reed, JS Del Cid, BC Hann, S Yang, A Giddabasap, K Noorbehesh, B Yang, J Dal Porto, T Tsukui, K Niessen, A Atakilit, RJ Akhurst, D Sheppard Integrin &amp;alphav&amp;beta8 on T�cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy Cell Reports, 2021-07-06;36(1):109309. 2021-07-06 [PMID: 34233193] (In Vivo, Mouse)
• SK Ho, Z Xu, A Thakur, M Fox, SS Tan, E DiGiammari, L Zhou, M Sho, B Cairns, V Zhao, M Xiong, J Samayoa, CM Forsyth, DB Powers, DT Chao, D Hollenbaug, HM Alvarez, Y Akamatsu Epitope and Fc-mediated Crosslinking, but not High Affinity, Are Critical for Antitumor Activity of CD137 Agonist Antibody with Reduced Liver Toxicity Mol. Cancer Ther., 2020-01-23;0(0):. 2020-01-23 [PMID: 31974274] (Agonist Activity, Mouse)
• Yiping Lu, Chao Li, Sitong Du, Xi Chen, Xinning Zeng, Fangwei Liu et al. 4-1BB Signaling Promotes Alveolar Macrophages-Mediated Pro-Fibrotic Responses and Crystalline Silica-Induced Pulmonary Fibrosis in Mice Frontiers in Immunology 2018-09-10 [PMID: 30250465]
• Helena Escuin-Ordinas, Mark W Elliott, Mohammad Atefi, Michelle Lee, Charles Ng, Liu Wei et al. PET imaging to non-invasively study immune activation leading to antitumor responses with a 4-1BB agonistic antibody Journal for ImmunoTherapy of Cancer 2013-08-27 [PMID: 24829750] (In vivo assay, Mouse)
• Wells JW, Cowled CJ, Farzaneh F Combined triggering of dendritic cell receptors results in synergistic activation and potent cytotoxic immunity. J. Immunol., 2008-09-01;181(5):3422-31. 2008-09-01 [PMID: 18714014] (Neutralization, Mouse)
• Wu T, Xie C, Bhaskarabhatla M, Yan M, Leone A, Chen SS, Zhou XJ, Putterman C, Mohan C Excreted urinary mediators in an animal model of experimental immune nephritis with potential pathogenic significance. Arthritis Rheum., 2007-03-01;56(3):949-59. 2007-03-01 [PMID: 17328072] (Control, Mouse)

Reviews for 4-1BB/TNFRSF9/CD137 Antibody (MAB9371) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer ELISA Mouse 04/07/2019

Summary

• Application: ELISA
• Sample Tested: COMMERCIAL ANTIGEN,M41BB ANTIGEN
• Species: Mouse
• Lot: IMF0216091

reviewed by: Verified Customer ELISA Mouse 10/16/2018

Summary

• Application: ELISA
• Sample Tested: commercial Antigen
• Species: Mouse
• Lot: IMF0216091

Bioinformatics

• Gene Symbol: TNFRSF9
• Uniprot:
  • P20334()