2B4/CD244/SLAMF4 [p Tyr271] Antibody

Western Blot: 2B4/CD244/SLAMF4 [p Tyr271] Antibody [NBP1-46104] - Analysis of IP from a lysate of Human Natural Killer cells with (+) or without (-) pervanadate treatment of the cells using this antibody at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Dr Nicholas, Sir William Dunn.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

2B4/CD244/SLAMF4 [p Tyr271] Antibody Summary

• Description: Novus Biologicals Goat 2B4/CD244/SLAMF4 [p Tyr271] Antibody (NBP1-46104) is a polyclonal antibody validated for use in WB and ELISA. Anti-2B4/CD244/SLAMF4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EFLTIpYEDVKD corresponding to internal region according to NP_057466.1.
• Epitope: internal region
• Modification: p Tyr271
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD244
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in human Natural Killer (NK) lysates after immunoprecipitation with mouse monoclonal antibody C1.7 coupled to Protein G agarose (calculated MW of 41.1 kDa band according to NP_057466.1). We do note that CD244 is a glycoprotein which makes a higher size band to be expected.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 2B4/CD244/SLAMF4 [p Tyr271] Antibody

• 2B4
• CD244 antigen
• CD244 molecule, natural killer cell receptor 2B4
• CD244
• h2B4
• NAIL
• NAILNK cell activation-inducing ligand
• natural killer cell receptor 2B4
• NKR2B4
• NKR2B4NK cell type I receptor protein 2B4,2B4CD244 natural killer cell receptor 2B4
• Nmrk
• SLAMF4
• SLAMF4NK cell activation inducing ligand NAIL

Background

2B4, also known as CD244 and SLAMF4, is a type I transmembrane glycoprotein that is member of the signaling lymphocyte activation molecule (SLAM) subfamily in immunoglobulin (Ig) superfamily (1,2). The 2B4 protein plays a role in immune system associated diseases including the recognition and clearance of cancer cells, infected cells, and key allergy cells (1). 2B4 is expressed as a heterophilic receptor on the cell membrane of natural killer (NK) cells in addition to on monocytes and basophils, gamma/delta T cells, a subset of CD8+ T cells, and other immune cells and tissues (1). Additionally, 2B4 is considered a marker for CD8+ T cell exhaustion in chronic infection and increased expression has been observed in diseases including lymphocytic choriomeningitis virus (LCMV) and hepatitis B virus (HBV) (2). Human 2B4 protein is 370 amino acids (aa) in length with a theoretical molecular weight (MW) of 41 kDa (3). However, 2B4 contains multiple glycosylation sites that increases its observed MW to ~70 kDa (3). The 2B4/CD244 protein consists of a 21 aa signal peptide followed by an extracellular domain (ECD) with two Ig-like domains, a single transmembrane domain, and four cytoplasmic immunoreceptor tyrosine-based switch motifs (ITSMs) (3).

The 2B4 receptor has both activating and inhibitory signaling properties (1,4). 2B4's ligand is CD48, which is expressed on hematopoietic cells (4, 5). When bound to CD48, subsequent phosphorylation of the ITSMs occurs, followed by recruitment of SH-2-containing adaptor protein (SAP) and Fyn, leading to phosphorylation of PLCgamma and Vav1, and resulting in NK cell activation (4). In the absence of SAP, Src homology region 2-containing protein tyrosine phosphatases (SHP) get recruited and inhibits NK cell activation (4). In the context of infection such as Epstein-Barr virus (EBV), when lacking SAP, 2B4 has been shown to have an inhibitory function via impaired T cell cytotoxicity against EBV-infected cells (6). While 2B4 has a role in eliciting CD8+ T cell response, overexpression of 2B4 can lead to T cell exhaustion in states of chronic infection (6). Studies have suggested that the antibody blockade of the 2B4-CD48 interaction might enhance T cell and NK cell function, however given both the activating and inhibitory properties of the 2B4 receptor, more research needs to be done to prevent unwanted effects (6).

References

1. Buller CW, Mathew PA, Mathew SO. Roles of NK Cell Receptors 2B4 (CD244), CS1 (CD319), and LLT1 (CLEC2D) in Cancer. Cancers (Basel). 2020 Jul 1;12(7):1755. https://doi.org/10.3390/cancers12071755

2. Catakovic K, Klieser E, Neureiter D, Geisberger R. T cell exhaustion: from pathophysiological basics to tumor immunotherapy. Cell Commun Signal. 2017;15(1):1. https://doi.org/10.1186/s12964-016-0160-z

3. Uniprot (Q9BZW8)

4. Chen Y, Lu D, Churov A, Fu R. Research Progress on NK Cell Receptors and Their Signaling Pathways. Mediators Inflamm. 2020 Jul 24;2020:6437057. https://doi.org/10.1155/2020/6437057.

5. Pahima H, Puzzovio PG, Levi-Schaffer F. 2B4 and CD48: A powerful couple of the immune system. Clin Immunol. 2019 Jul;204:64-68. https://doi.org/10.1016/j.clim.2018.10.014.

6. Waggoner SN, Kumar V. Evolving role of 2B4/CD244 in T and NK cell responses during virus infection. Front Immunol. 2012;3:377. https://doi.org/10.3389/fimmu.2012.00377

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 2B4/CD244/SLAMF4 Antibody (NBP1-46104)(1)

Publication using NBP1-46104

• Tangye SG, Lazetic S, Woollatt E et al. Cutting edge: human 2B4, an activating NK cell receptor, recruits the proteintyrosine phosphatase SHP-2 and the adaptor signaling protein SAP. J Immunol 1999-06-15 [PMID: 10358138]

Bioinformatics

• Gene Symbol: CD244
• Entrez:
  • 51744(Human)
• Uniprot:
  • NP_057466.1(Human)