15-PGDH/HPGD Antibody - BSA Free

Western Blot: 15-PGDH/HPGD Antibody [NBP2-21588] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody [NBP2-21588] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP2-21588] - Human colon carcinoma, using 15-PGDH antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Po, Bv, Ca
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

15-PGDH/HPGD Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human 15-PGDH/HPGD. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Porcine (93%), Bovine (91%), Canine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HPGD
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%), Guinea pig (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for 15-PGDH/HPGD Antibody - BSA Free

• 15-hydroxyprostaglandin dehydrogenase [NAD+]
• 15PGDH
• 15-PGDH
• EC 1.1.1
• EC 1.1.1.141
• HPGD
• hydroxyprostaglandin dehydrogenase 15-(NAD)
• NAD+-dependent 15-hydroxyprostaglandin dehydrogenase
• PGDH1
• PGDH1PGDH
• Prostaglandin dehydrogenase 1
• SDR36C1
• short chain dehydrogenase/reductase family 36C, member 1

Background

This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: HPGD
• Uniprot:
  • P15428(Human)