15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free

Western Blot: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15-PGDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15-PGDH.

Immunohistochemistry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - Staining of Human bladder tissue using anti-15-PGDH mouse monoclonal antibody.

Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-15-PGDH antibody, and then analyzed by flow cytometry.

Immunohistochemistry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - Staining of Adenocarcinoma of Human endometrium tissue using anti-15-PGDH mouse monoclonal antibody.

Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - Analysis of Hela cells, using anti-15-PGDH antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - Analysis of Jurkat cells, using anti-15-PGDH antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI2C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free (NBP2-00490) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human HPGD (NP_000851) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HPGD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 28.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free

• 15-hydroxyprostaglandin dehydrogenase [NAD+]
• 15PGDH
• 15-PGDH
• EC 1.1.1
• EC 1.1.1.141
• HPGD
• hydroxyprostaglandin dehydrogenase 15-(NAD)
• NAD+-dependent 15-hydroxyprostaglandin dehydrogenase
• PGDH1
• PGDH1PGDH
• Prostaglandin dehydrogenase 1
• SDR36C1
• short chain dehydrogenase/reductase family 36C, member 1

Background

15-PGDH (15-hydroxyprostaglandin dehydrogenase (NAD+)) is responsible for prostaglandin inactivation and supports the regulation of functions that are controled by prostaglandin levels. Specifically, 15PGDH catalyzes the chemical reaction to convert NAD+ to NADH and H+.

15-PGDH is a novel tumor suppressor in the COX-2 pathway. 15-PGDH is found in many normal tissues and appears to be down-regulated in colorectal and lung carcinoma tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HPGD