14-3-3 eta Antibody

Western Blot: 14-3-3 eta Antibody [NBP2-21586] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 eta antibody [N1C3] (NBP2-21586) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ch, Rb, Xp
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

14-3-3 eta Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human 14-3-3 eta. The exact sequence is proprietary.
• Predicted Species: Mouse (98%), Rat (98%), Chicken (97%), Xenopus (91%), Bovine (98%), Rabbit (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: YWHAH
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000-1:20000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (91%), Zebrafish (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for 14-3-3 eta Antibody

• 1433 eta
• 14-3-3 eta
• 14-3-3 protein eta
• Protein AS1
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, etapolypeptide
• YWHA1
• YWHA114-3-3 eta
• YWHAH

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat has been associated with early-onset schizophrenia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: YWHAH
• Uniprot:
  • Q04917()