14-3-3 eta Antibody (3G12) [FITC]

• Reactivity: Hu, Mu, Rt, Bv, Ca, Eq
• Applications: WB, ICC/IF, IHC, KO
• Clone: 3G12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

14-3-3 eta Antibody (3G12) [FITC] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: Full length recombinant human 14-3-3 eta expressed in and purified from E.coli [UniProt# Q04917].
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: YWHAH
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Knockout Validated
  • Western Blot
• Application Notes: This 14-3-3 eta (3G12) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 28 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for 14-3-3 eta Antibody (3G12) [FITC]

• 1433 eta
• 14-3-3 eta
• 14-3-3 protein eta
• Protein AS1
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, etapolypeptide
• YWHA1
• YWHA114-3-3 eta
• YWHAH

Background

14-3-3 proteins are highly conserved and ubiquitously expressed proteins present as multigene families in most of the living organisms wherein they play a key role of bringing signal transduction to completion, and implicates in regulating several cellular processes including apoptosis, cell-cycle checkpoints and nutrient-sensing signaling pathways. 14-3-3 proteins have several isoforms which include -beta, -gamma, -epsilon, -sigma, -zeta, -tau, and -eta. 14-3-3 proteins can form homo- or heterodimers which interacts with a wide variety of proteins such as transcription factors, enzymes/kinases, cytoskeletal proteins, phosphatases, and other signaling molecules. 14-3-3 interactions with its targets occurs primarily via phospho-Ser/Thr motif, however, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions have been shown. 14-3-3 binding masks specific sequences of target proteins, thereby altering their localization, phosphorylation state, stability, and molecular interactions, and also induces target protein conformational changes which results in altered target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in developmental and disease signaling which underlies their different functions despite sequence similarities. 14-3-3 eta has been shown to accumulate in CSF of Creutzfeld Jacob Disease patients; bind to alpha-synuclein in Lewy bodies of Parkinson's disease, and has been linked to early-onset Schizophrenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YWHAH