14-3-3 eta Antibody (3G12) [FITC]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF
Clone
3G12
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

14-3-3 eta Antibody (3G12) [FITC] Summary

Immunogen
Full length recombinant human 14-3-3 eta [UniProt# Q04917].
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
YWHAH
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
Application Notes
This 14-3-3 eta (3G12) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 28 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for 14-3-3 eta Antibody (3G12) [FITC]

  • 1433 eta
  • 14-3-3 eta
  • 14-3-3 protein eta
  • Protein AS1
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, etapolypeptide
  • YWHA1
  • YWHA114-3-3 eta
  • YWHAH

Background

14-3-3 proteins are highly conserved and ubiquitously expressed proteins present as multigene families in most of the living organisms wherein they play a key role of bringing signal transduction to completion, and implicates in regulating several cellular processes including apoptosis, cell-cycle checkpoints and nutrient-sensing signaling pathways. 14-3-3 proteins have several isoforms which include -beta, -gamma, -epsilon, -sigma, -zeta, -tau, and -eta. 14-3-3 proteins can form homo- or heterodimers which interacts with a wide variety of proteins such as transcription factors, enzymes/kinases, cytoskeletal proteins, phosphatases, and other signaling molecules. 14-3-3 interactions with its targets occurs primarily via phospho-Ser/Thr motif, however, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions have been shown. 14-3-3 binding masks specific sequences of target proteins, thereby altering their localization, phosphorylation state, stability, and molecular interactions, and also induces target protein conformational changes which results in altered target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in developmental and disease signaling which underlies their different functions despite sequence similarities. 14-3-3 eta has been shown to accumulate in CSF of Creutzfeld Jacob Disease patients; bind to alpha-synuclein in Lewy bodies of Parkinson's disease, and has been linked to early-onset Schizophrenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 350 Labeled NBP1-92691UV
DyLight 405 Labeled NBP1-92691V
DyLight 488 Labeled NBP1-92691G
DyLight 680 Labeled NBP1-92691FR
DyLight 755 Labeled NBP1-92691IR

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Bioinformatics Tool for 14-3-3 eta Antibody (NBP1-92691F)

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Bioinformatics

Gene Symbol YWHAH
Entrez