14-3-3 eta Antibody (3G12) [PE]

• Reactivity: Hu, Mu, Rt, Bv, Ca, Eq
• Applications: WB, ICC/IF, IHC, KO
• Clone: 3G12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

14-3-3 eta Antibody (3G12) [PE] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: Full length recombinant human 14-3-3 eta expressed in and purified from E.coli [UniProt# Q04917].
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: YWHAH
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Knockout Validated
  • Western Blot
• Application Notes: This 14-3-3 eta (3G12) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 28 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for 14-3-3 eta Antibody (3G12) [PE]

• 1433 eta
• 14-3-3 eta
• 14-3-3 protein eta
• Protein AS1
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, etapolypeptide
• YWHA1
• YWHA114-3-3 eta
• YWHAH

Background

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YWHAH