14-3-3 beta/alpha Antibody - Azide Free

Western Blot: 14-3-3 beta/alpha Antibody [NB100-665]

• Reactivity: Rt, Hu, Mu, Bv, Ca, Ch, Pm, Xp, Ze
• Applications: WB, IHC-P (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

14-3-3 beta/alpha Antibody - Azide Free Summary

• Immunogen: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # P35213
• Localization: Cytoplasmic
• Specificity: Specific for endogenous levels of the ~29 kDa 14-3-3 protein. Immunolabeling is blocked by preadsorption of the antibody with the peptide immunogen.
• Predicted Species: Human (100%), Mouse (100%), Zebrafish (100%), Bovine (100%), Chicken (100%), Canine (100%), Primate (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: YWHAB
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Application Notes: This antibody has been tested in Western blot on rat brain hippocampal lysate, detecting a band ~29 kDa.
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  14-3-3 tau/theta Overexpression Lysate

Reactivity Notes

It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primates, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for 14-3-3 beta/alpha Antibody - Azide Free

• 14-3-3 alpha
• 14-3-3 beta
• 14-3-3 protein beta/alpha
• brain protein 14-3-3, beta isoform
• GW128
• HS1
• KCIP-1
• Protein 1054
• Protein kinase C inhibitor protein 1
• protein kinase C inhibitor protein-1
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alphapolypeptide
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, betapolypeptide
• YWHAA

Background

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions. 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for 14-3-3 beta/alpha Antibody (NB100-665) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Astrid Spannhoff WB Mouse 11/19/2010

Summary

• Application: Western Blot
• Sample Tested: Mouse brain extract, Sample Amount: 10ul
• Species: Mouse

Bioinformatics

• Gene Symbol: YWHAB
• Entrez:
  • 7529(Human)
  • 54401(Mouse)
  • 56011(Rat)
• Uniprot:
  • P35213(Rat)