14-3-3 beta/alpha Antibody

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Western Blot: 14-3-3 beta/alpha Antibody [NB100-665]

Product Details

Summary
Reactivity Rt, Hu, Mu, Bv, Ca, Ch, Pm, Xp, ZeSpecies Glossary
Applications WB, IHC-P (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

14-3-3 beta/alpha Antibody Summary

Immunogen
Peptide corresponding to amino acid residues from the N-terminal region of the rat 14-3-3 protein, conjugated to keyhole limpet hemocyanin (KLH).
Localization
Cytoplasmic
Specificity
Specific for the ~29k 14-3-3 beta/alpha protein. Immunolabeling of the 14-3-3 beta/alpha protein band is completely blocked by pre-adsorption of the antibody with the C-terminal peptide that was used to generate the antibody.
Predicted Species
Human (100%), Mouse (100%), Zebrafish (100%), Bovine (100%), Canine (100%), Chicken (100%), Xenopus (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
YWHAB
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
This antibody has been tested in Western blot on rat brain hippocampal lysate, detecting a band ~29 kDa.
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
14-3-3 tau/theta Lysate (NBL1-17951)
Reviewed Applications
Read 1 Review rated 4
using
NB100-665 in the following applications:

Reactivity Notes

It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primates, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for 14-3-3 beta/alpha Antibody

  • 14-3-3 alpha
  • 14-3-3 beta
  • 14-3-3 protein beta/alpha
  • brain protein 14-3-3, beta isoform
  • GW128
  • HS1
  • KCIP-1
  • Protein 1054
  • Protein kinase C inhibitor protein 1
  • protein kinase C inhibitor protein-1
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alphapolypeptide
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, betapolypeptide
  • YWHAA

Background

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions. 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC-P (-)

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Review for 14-3-3 beta/alpha Antibody (NB100-665) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB100-665:
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Western Blot 14-3-3 beta/alpha NB100-665
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reviewed by:
Astrid Spannhoff
WB Mouse 11/19/2010
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Summary

ApplicationWestern Blot
Sample TestedMouse brain extract, Sample Amount: 10ul
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: 20 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: 5% Milk in PBST, Incubation Time: overnight, Incubation Temp: 20 C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-rabbit IgG, HRP-label, Secondary Ab Dilution Ratio: 1:5000
Secondary Manufacturer Cat#NA9340

Details

Detection NotesDetection Method: ECL, Exposure Time: 1 minute, Specific Bands: ~29 kDa, Non-Specific Bands: ~33, 24 kDa

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Astrid Spannhoff
11/19/2010
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol YWHAB
Entrez
Uniprot