14-3-3 beta/alpha Antibody (S07-1A8)

Western Blot: 14-3-3 beta/alpha Antibody (S07-1A8) [NBP3-14905] - Western blot detection of 41701 in K562, Rat Brain, C6, 3T3, Hela cell lysates using 14-3-3 beta/alpha Rabbit mAb (1:1000 diluted). Predicted band size: 28kDa. Observed band size: 28kDa.

Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody (S07-1A8) [NBP3-14905] - Immunohistochemistry of 14-3-3 beta/alpha in paraffin-embedded Human tonsil using 14-3-3 beta/alpha Rabbit mAb at dilution 1/100

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: S07-1A8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

14-3-3 beta/alpha Antibody (S07-1A8) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human 14-3-3 beta/alpha (Uniprot#: P31946)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: YWHAB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for 14-3-3 beta/alpha Antibody (S07-1A8)

• 14-3-3 alpha
• 14-3-3 beta
• 14-3-3 protein beta/alpha
• brain protein 14-3-3, beta isoform
• GW128
• HS1
• KCIP-1
• Protein 1054
• Protein kinase C inhibitor protein 1
• protein kinase C inhibitor protein-1
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alphapolypeptide
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, betapolypeptide
• YWHAA

Background

The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints. The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YWHAB