14-3-3 beta/alpha Antibody (S07-1A3)

Western Blot: 14-3-3 beta/alpha Antibody (S07-1A3) [NBP3-19940] - Western blot detection of 14-3-3 beta/alpha in Jurkat, Rat Brain, C6, CHO-K1 cell lysates using NBP3-19940 (1:500 diluted). Predicted band size: 28kDa. Observed band size: 27-29kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S07-1A3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

14-3-3 beta/alpha Antibody (S07-1A3) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human 14-3-3 beta/alpha (Uniprot # P31946)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: YWHAB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for 14-3-3 beta/alpha Antibody (S07-1A3)

• 14-3-3 alpha
• 14-3-3 beta
• 14-3-3 protein beta/alpha
• brain protein 14-3-3, beta isoform
• GW128
• HS1
• KCIP-1
• Protein 1054
• Protein kinase C inhibitor protein 1
• protein kinase C inhibitor protein-1
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alphapolypeptide
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, betapolypeptide
• YWHAA

Background

The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints. The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YWHAB