11 beta-HSD1 Antibody

Western blot shows lysates of human, mouse and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat 11 beta -HSD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3397) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for 11 beta -HSD1 at approximately 36 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Effect of S100A16 on 11 beta -HSD1 expression Adipose, liver, and muscle tissues were removed from C57BL/6, S100A16Tg/Tg, and S100A16KO/+ mice, and proteins were extracted. (A) Analysis of S100A16 and 11 beta -HSD1 protein levels by Western blotting. (B) Relative expression of S100A16 and 11 beta -HSD1 based on grayscale analysis with alpha -tubulin as a control. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31399502), licensed under a CC-BY license. Not internally tested by R&D Systems.

Lipid and triglyceride content increased in Hep1-6 cells that overexpressed 11 beta -HSD1. (A) Western blot showing the expression of 11 beta -HSD1 and (B) relative protein levels (n = 5). (C) Oil red O staining of Hep1-6 cells with 5% BSA or 300 μM OA/PA treatment. Box indicates magnified field of view. (D) Measurement of triglyceride content in Hep1-6 cells with the same treatment as (C) (n = 3). **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35637957), licensed under a CC-BY license. Not internally tested by R&D Systems.

Lipogenesis pathway is activated by overexpression of 11 beta -HSD1. (A) Western blot detection of proteins related to the lipid biosynthesis, oxidation, secretion, uptake, and hydrolysis pathways in 11 beta -HSD1 overexpressed or GFP overexpressed Hep1-6 cells exposed or not to 0.25 μM BVT2733 and (B) relative protein levels (n = 3). (C) Oil red O staining of Hep1-6 cells and treatments from (A). (D) Measurement of triglyceride levels in Hep1-6 cells and treatments from (A) (n = 3). (E) Western blot showing the expression levels of lipogenesis pathway regulatory proteins in Hep1-6 cells and treatments from (A), and (F) relative protein levels (n = 3). *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35637957), licensed under a CC-BY license. Not internally tested by R&D Systems.

The phenotype of hepatocyte-specific 11 beta -HSD1 transgenic mice (TGM). (A) Western blot showing the expression of 11 beta -HSD1 in liver of Con vs TGM (n = 3). (B) Body weights (n = 10). (C) Liver weights (n = 10). (D) The statistical analysis of liver weight/body weight ratios (n = 10). (E) Oil red O staining of liver tissues. (F) Measurement of triglyceride levels in livers of Con vs TGM (n = 8). (G) Western blot detection of proteins related to lipogenesis and (H) relative protein levels (n = 3). (I) Western blot showing the expression levels of the GR and p-GR proteins in the nucleus and cytoplasm. *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35637957), licensed under a CC-BY license. Not internally tested by R&D Systems.

Lipogenesis pathway is activated in primary hepatocytes derived from TGM. (A) Oil red O staining of primary hepatocytes of Con vs TGM. Scale bars: 50 μm. (B) Measurement of triglyceride content in primary hepatocytes from Con vs TGM (n = 3). (C) Western blot showing the expression levels of lipogenesis pathway regulatory proteins in primary hepatocytes of Con vs TGM, treated or not with 0.25 μM BVT2733 and (D) relative protein levels (n = 3). *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35637957), licensed under a CC-BY license. Not internally tested by R&D Systems.

Lipid and triglyceride content increased in Hep1-6 cells that overexpressed 11 beta -HSD1. (A) Western blot showing the expression of 11 beta -HSD1 and (B) relative protein levels (n = 5). (C) Oil red O staining of Hep1-6 cells with 5% BSA or 300 μM OA/PA treatment. Box indicates magnified field of view. (D) Measurement of triglyceride content in Hep1-6 cells with the same treatment as (C) (n = 3). **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35637957), licensed under a CC-BY license. Not internally tested by R&D Systems.

The phenotype of hepatocyte-specific 11 beta -HSD1 transgenic mice (TGM). (A) Western blot showing the expression of 11 beta -HSD1 in liver of Con vs TGM (n = 3). (B) Body weights (n = 10). (C) Liver weights (n = 10). (D) The statistical analysis of liver weight/body weight ratios (n = 10). (E) Oil red O staining of liver tissues. (F) Measurement of triglyceride levels in livers of Con vs TGM (n = 8). (G) Western blot detection of proteins related to lipogenesis and (H) relative protein levels (n = 3). (I) Western blot showing the expression levels of the GR and p-GR proteins in the nucleus and cytoplasm. *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35637957), licensed under a CC-BY license. Not internally tested by R&D Systems.

Lipogenesis pathway is activated in primary hepatocytes derived from TGM. (A) Oil red O staining of primary hepatocytes of Con vs TGM. Scale bars: 50 μm. (B) Measurement of triglyceride content in primary hepatocytes from Con vs TGM (n = 3). (C) Western blot showing the expression levels of lipogenesis pathway regulatory proteins in primary hepatocytes of Con vs TGM, treated or not with 0.25 μM BVT2733 and (D) relative protein levels (n = 3). *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35637957), licensed under a CC-BY license. Not internally tested by R&D Systems.

Lipogenesis pathway is activated by overexpression of 11 beta -HSD1. (A) Western blot detection of proteins related to the lipid biosynthesis, oxidation, secretion, uptake, and hydrolysis pathways in 11 beta -HSD1 overexpressed or GFP overexpressed Hep1-6 cells exposed or not to 0.25 μM BVT2733 and (B) relative protein levels (n = 3). (C) Oil red O staining of Hep1-6 cells and treatments from (A). (D) Measurement of triglyceride levels in Hep1-6 cells and treatments from (A) (n = 3). (E) Western blot showing the expression levels of lipogenesis pathway regulatory proteins in Hep1-6 cells and treatments from (A), and (F) relative protein levels (n = 3). *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35637957), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

11 beta-HSD1 Antibody Summary

• Immunogen: E. coli-derived recombinant human 11 beta -HSD1
  Asn24-Lys292
  Accession # P28845
• Specificity: Detects endogenous human, mouse and rat 11 beta -HSD1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HSD11B1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 11 beta-HSD1 Antibody

• 11 betaHSD1
• 11 beta-HSD1
• 11-beta-hydroxysteroid dehydrogenase 1
• EC 1.1.1
• EC 1.1.1.146
• HDL
• HSD11B1
• HSD11HSD11B
• HSD11LMGC13539
• hydroxysteroid (11-beta) dehydrogenase 1
• member 1
• SDR26C1

Background

Hydoxysteroid (11-beta) Dehydrogenase, type I 11-beta-hydroxysteroid dehydrogenase 1(11 beta -HSD1, HSD11B1 or HSD1) is a microsomal glycoprotein enzyme, with an apparent mass of 36 kDa, that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. 11 beta -HSD1 can also catalyze the reverse reaction, the conversion of cortisone to cortisol. 11 beta -HSD1 has been detected in a wide range of rat and human tissues, including lung and testis, but highest amounts are expressed in the liver. Human 11 beta -HSD1 shares 79 and 77% sequence identity with mouse and rat 11 beta -HSD1, respectively. There are at least 2 isoforms of 11 beta -HSD. The other isoform, type II, is expressed predominantly in the kidney and placenta and catalyzes only the 11-beta-dehydrogenation reaction. High levels of cortisol can lead to visceral obesity and particular defects in the expression or function of 11 beta -HSD1 have also been associated with insulin resistance and cognitive function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 11 beta-HSD1 Antibody (AF3397)(7)

Publications using AF3397

• Mengliang Hu, Tingting Han, Qiyu Pan, Dongsheng Ni, Fengyi Gao, Liying Wang et al. The GR-gp78 Pathway is involved in Hepatic Lipid Accumulation Induced by Overexpression of 11 beta -HSD1 International Journal of Biological Sciences 4/30/2022 [PMID: 35637957]
• V Tesic, J Ciric, I Jovanovic, N Zogovic, D Milanovic, S Kanazir, M Perovic Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction Nutrients, 2021-12-17;13(12):. 2021-12-17 [PMID: 34960078] (Western Blot, Rat)
• HC Huang, MH Tsai, FY Lee, TY Lin, CC Chang, CL Chuang, SJ Hsu, MC Hou, YH Huang NAFLD Aggravates Septic Shock Due to Inadequate Adrenal Response and 11beta-HSDs Dysregulation in Rats Pharmaceutics, 2020-04-28;12(5):. 2020-04-28 [PMID: 32354071] (Western Blot, Rat)
• Rihua Zhang, Jing Bao Kan, Shan Lu, Pei Tong, Jie Yang, Ling Xi et al. S100A16-induced adipogenesis is associated with up-regulation of 11 beta -hydroxysteroid dehydrogenase type 1 (11 beta -HSD1) Bioscience Reports 9/30/2019 [PMID: 31399502]
• Rihua Zhang, Jing Bao Kan, Shan Lu, Pei Tong, Jie Yang, Ling Xi et al. S100A16-induced adipogenesis is associated with up-regulation of 11 beta -hydroxysteroid dehydrogenase type 1 (11 beta -HSD1) Bioscience Reports 2019-09-30 [PMID: 31399502] (Western Blot, Mouse)
• Petrus P, Rosqvist F, Edholm D, Mejhert N, Arner P, Dahlman I, Ryden M, Sundbom M, Riserus U Saturated fatty acids in human visceral adipose tissue are associated with increased 11- beta-hydroxysteroid-dehydrogenase type 1 expression. Lipids Health Dis, 2015-05-02;14(0):42. 2015-05-02 [PMID: 25934644] (Western Blot, Human)
• Li B, Yau P, Kemper B Identification of cytochrome P450 2C2 protein complexes in mouse liver. Proteomics, 2011-08-01;11(16):3359-68. 2011-08-01 [PMID: 21751364] (Immunoprecipitation, Rabbit)

Bioinformatics

• Gene Symbol: HSD11B1
• Uniprot:
  • P28845()