| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-308-1mg | |||
| BSA Free | NB600-308SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB600-1502 | |||
| Biotin | NB100-1678 | |||
| DyLight 488 | NBP1-69969 | |||
| FITC | NB100-1771 | |||
| FITC | NB100-1771SS | |||
| HRP | NB600-313 | |||
| Rhodamine | NBP1-97327-500ug | |||
| Texas Red | NB120-6660 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1770SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1614-0.02ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB110-40670 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-597SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB100-1365 | |||
| FITC | NB100-1183-0.025mg | |||
| FITC | NB100-1183 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22111SS | |||
| Azide and BSA Free | NBP2-80750 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | IC4240A | |||
| Phycoerythrin | IC4240P | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC4240P | |||
| Carrier Free | IC4240A | |||
| Carrier Free | AF4240-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-62622-0.1ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37821-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC42402T-100UG | |||
| Carrier Free | IC42402R-100UG | |||
| Carrier Free | IC42402U-100UG | |||
| Carrier Free | IC42402V-100UG | |||
| Carrier Free | IC42402G-100UG | |||
| Carrier Free | IC42402N-100UG | |||
| Carrier Free | IC42402S-100UG | |||
| Carrier Free | MAB42402-SP | |||
![Total cell lysates (20ug) with protein of interest tagged with GFP was loaded. Blotted with GFP antibody [HRP] NB600-308H, without any secondary antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/GFP-Antibody-Western-Blot-NB600-308-img0036.jpg)
![Western Blot of GFP antibody. Lane 1: Wild type GFP (0.1 ug) was used to spike HeLa whole cell lysate. Lane 2: none. Load: 30 ug per lane.Primary antibody: GFP antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) Goat-a-Rabbit IgG [H&L] MX10 at 1:10,000 for 45 min at RT.Block: 5% BLOTTO in PBS overnight at 4C.Predicted/Observed size: 27 kDa for epitope tag GFP. Other band(s): none.](http://images.novusbio.com/fullsize/GFP-Antibody-Western-Blot-NB600-308-img0032.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-308] - Myc is post-transcriptionally regulated by the IIS-GSK3 cascade.(A) Germaria from ovaries of ctrl, chicoRNAi, & bskRNAi driven by nos-Gal4 expressing LacZ under the control of myc endogenous promoter stained with anti-beta -galactosidase & anti-1B1. Scale bars, 10 μm. (B) A representative live imaging of germaria from flies expressing GFP-tagged Sgg under its endogenous promoter. Scale bar, 10 μm. (C) A germarium from wt flies endogenously expressing Myc-GFP stained with anti-GFP (green) & anti-p-GSK3 (red). Germarium regions are indicated. Myc protein highly corresponds to inhibited form of GSK3. Scale bar, 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31612862), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-308_rabbit-polyclonal-gfp-antibody-31020241529193.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-308] - Myc protein pattern in the ovary.(A) A representative low-magnification image of ovarioles from flies endogenously expressing Myc-GFP stained with anti-GFP, anti-1B1, & DAPI. Note that Myc level is low in early germarium stages, becomes high from germarium region 2B, & reduces from the stage-10 egg chamber. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31612862), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-308_rabbit-polyclonal-gfp-antibody-310202415291968.jpg)
![Western Blot: GFP Antibody [NB600-308] - MISP interacts & co-localizes with IQGAP1. (a) HEK293T cells transiently overexpressing GFP-IQGAP1 & FLAG-MISP were used for co-immunoprecipitation experiments from both sides (GFP trap or FLAG M2 beads). (b) Endogenous immunoprecipitation using IQGAP1 antibody or control immunoglobulin (IgG) was carried out in HeLa cell lysates & MISP was detected in the eluate. (c) In vitro interaction between purified MBP-MISP & GST-IQGAP1 was detected by GST & MBP pull-down experiments. (d) Immunoprecipiation experiments showing that GFP-MISP binds endogenous IQGAP1 (upper panel) & MISP binds to IQGAP1 endogenously using specific antibodies (lower panel) in mitotically arrested (taxol & nocodazole, respectively) HeLa cell lysates. (a–d) Images were gained from the same Western blot membrane after cutting/cropping & presented with different exposure times or contrast enhancement for better presentation purposes. The dividing lane marks the grouping of images of the same (or different) membrane. Uncropped blots, where applicable, are included in Supplementary Fig. 4. (e) HeLa cells were immunostained for MISP & IQGAP1 & co-localization was visualized in mitosis in single-plane confocal images, scale bar: 5 μm. Lower pictures: After manual thresholding white spots mark the co-localizing areas in the merge images. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29679050), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-308_rabbit-polyclonal-gfp-antibody-310202416173520.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-308] - BRC-1 & BRD-1 are inter-dependent for localization.A) Co-localization between BRD-1::GFP (green) & TagRFP-T::BRC-1 (red) at late pachytene in live worms. Scale bar = 10 μm. B) Stills of germline nuclei from live worms expressing GFP::BRC-1 & mCherry::Histone H2B (mCherry::his-58; gfp::brc-1); merge & GFP channel; top two panels, respectively. GFP::BRC-1 expression in brd-1(ok1623) mutant at indicated meiotic stages. Bottom two panels show BRD-1::GFP localization in wild type & the brc-1(xoe4) mutant. Images are projections through half of the gonad. TZ = transition zone; EP = early pachytene; MP = mid pachytene; LP = late pachytene; DP = diplotene; DK = diakinesis. Scale bar = 5 μm. C) Immunoblot of whole worm extracts from indicated worms probed with anti-GFP & alpha -tubulin antibodies. Lane 1 = N2: wild type; Lane 2 = JEL515: gfp::brc-1; Lane 3 = JEL520: gfp::brc-1 brd-1(ok1623); Lane 4 = JEL744: brc-1(xoe4) brd-1::gfp; Lane 5 = JEL657: brd-1::gfp; Lane 6 = JEL678: brc-1(tm1145) brd-1::gfp. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30383767), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-308_rabbit-polyclonal-gfp-antibody-310202416165637.jpg)
![Western Blot: GFP Antibody [NB600-308] - BRC-1 & BRD-1 are inter-dependent for localization.A) Co-localization between BRD-1::GFP (green) & TagRFP-T::BRC-1 (red) at late pachytene in live worms. Scale bar = 10 μm. B) Stills of germline nuclei from live worms expressing GFP::BRC-1 & mCherry::Histone H2B (mCherry::his-58; gfp::brc-1); merge & GFP channel; top two panels, respectively. GFP::BRC-1 expression in brd-1(ok1623) mutant at indicated meiotic stages. Bottom two panels show BRD-1::GFP localization in wild type & the brc-1(xoe4) mutant. Images are projections through half of the gonad. TZ = transition zone; EP = early pachytene; MP = mid pachytene; LP = late pachytene; DP = diplotene; DK = diakinesis. Scale bar = 5 μm. C) Immunoblot of whole worm extracts from indicated worms probed with anti-GFP & alpha -tubulin antibodies. Lane 1 = N2: wild type; Lane 2 = JEL515: gfp::brc-1; Lane 3 = JEL520: gfp::brc-1 brd-1(ok1623); Lane 4 = JEL744: brc-1(xoe4) brd-1::gfp; Lane 5 = JEL657: brd-1::gfp; Lane 6 = JEL678: brc-1(tm1145) brd-1::gfp. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30383767), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-308_rabbit-polyclonal-gfp-antibody-31020241616050.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-308] - Other IIS components are not altered by bsk RNAi.(A) Confocal images for germaria expressing Chico-GFP, myc-Dp110 or PDK1-GFP in the background of ctrl or bskRNAi from ovaries stained with anti-GFP or anti-myc. Scale bars, 10 μm. (B) Ovaries expressing LacZ driven by the puc promoter stained with anti-beta -galactosidase, anti-1B1, & DAPI. Note that JNK activity is moderately induced in late germarium & decreased in the following stages, while JNK activity is much stronger in follicle cells of a maturing egg. Scale bars, 20 μm. (C) Germaria from ovaries of ctrl & bskRNAi driven by nos-Gal4 stained with anti-AKT & anti-1B1. Scale bars, 10 μm. (D) Quantification of AKT intensity from cysts in germarium region 2A & 2B of ovaries with ctrl & bskRNAi driven by nos-Gal4. Intensities are normalized to the value of ctrl at region 2B. n = 7 & 8 germaria for ctrl & bskRNAi, respectively. Error bars represent SEM. *p<0.005. (E) Visualization of the InR mRNA by FISH in germaria from ovaries of ctrl & bskRNAi driven by nos-Gal4. Note that InR mRNA level is decreased by bsk RNAi. Germaria are outlined with dotted lines. Scale bars, 10 μm. (F) Quantification of InR mRNA density in region 2A & 2B germ cells from ovaries with indicated genotypes. n = 9 germaria for each genotype. Error bars represent SEM. *p<0.005. (G) Quantification of relative Myc-GFP intensity in region 2B cysts of ovaries illustrated in Figure 5H. n = 10 germaria for each genotype. Error bars represent SEM. *p<0.005.10.7554/eLife.49309.017Figure 5—figure supplement 1—source data 1.Relative AKT intensity, InR mRNA density, & Myc-GFP intensity in the germarium.Relative AKT intensity, InR mRNA density, & Myc-GFP intensity in the germarium. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31612862), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-308_rabbit-polyclonal-gfp-antibody-31020241616189.jpg)
![Western Blot: GFP Antibody [NB600-308] - GFP Expression within A. thaliana upon infiltration with SWNT-PM-CytKH9/pDNA complexes.a Reporter construct design of transient GFP expression for the nucleus & mitochondria. b Representative confocal laser scanning microscopy images of A. thaliana root cells from a minimum of five seedlings for each condition (n = 5) 18 h post infiltration with SWNT-PM-CytKH9/pDNA complexes containing pDONR-35S-GFP or pDONR-Cox2-GFP reporter constructs for nuclear or mitochondrial expression, respectively. Mitochondria were stained with MitoTracker Red (CMXRos), & colocalization analysis was performed on GFP expression & MitoTracker signals. Scale bars represent 20 μm. c, d Representative western blots & their quantification from cytosolic (c) & mitochondrial (d) fractions isolated from approximately 60 seedlings 18 h post infiltration with SWNT NCs probed for GFP expression. Actin & cytochrome c were used as loading controls for cytosolic & mitochondrial proteins, respectively. Data points from five biological replicates (n = 5) are represented as the mean ± standard deviation. Statistical significance was determined by Brown-Forsythe & Welch one-way ANOVA test. For the cytosolic samples, P-values are 0.0027, & 0.0020 between DNA only & SWNT-PM-CytKH9, SWNT-PM-Cytcox, respectively, & 0.0253 between SWNT-PM-CytKH9 & SWNT-PM-Cytcox. For the mitochondrial samples, P-values are 0.0019, & 0.0333 between DNA only & SWNT-PM-CytKH9, & SWNT-PM-Cytcox, respectively, & 0.0359 between SWNT-PM-CytKH9 & SWNT-PM-Cytcox. ns – not statistically significant, *P < 0.05, **P < 0.01. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35577779), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-308_rabbit-polyclonal-gfp-antibody-310202416165624.jpg)
![Western Blot: GFP Antibody [NB600-308] - VGLL1 regulates MMP9 transcription in gastric cancer cells. (a,b) Viability (a) & invasion (b) assay of NUGC3 cells. Cells were treated with 20 nM siScramble (SC) or siMMP9 for 48 h, & then stained with sulforhodamine B. Data are presented as mean ±SD. n = 3; * p < 0.05 (Student’s t-test). (c) MMP9 mRNA expression regulation by VGLL1 in gastric cancer cells treated with siVGLL1 or siSC (control) was measured by qPCR. (d) Changes in MMP9 expression upon knockdown or overexpression of VGLL1 assayed by western blotting in NUGC3 cells. (e) IHC of VGLL1 & MMP9 expression in the liver & lungs of an in vivo metastasis mouse model using surgical resection of tumors. Scale bar, 200 µm. (f) Construction of various luciferase reporter systems under control of the MMP9 promoter. (g) MMP9 promoter activities of the reporter systems containing modified TEA-binding sites were measured in NUGC3 cells. n = 3; * p < 0.05 (Student’s t-test). (h) Effect of TEAD4 on VGLL1-regulated MMP9 transcriptional activity. NUGC3 cells treated with siSC or siTEAD4 for 24 h were transfected with MMP9-luc, Renilla-luc, pCDNA3.1, & pcDNA3.1-myc-VGLL1 vectors for 48 h. n = 3; * p < 0.05 (Student’s t-test). (i) Interaction between VGLL1 & TEAD4. Lysates of NUGC3 cells that were transfected with pcDNA3.1-myc-VGLL1 & pEGFP-N1-TEAD4 were immunoprecipitated using anti-IgG, anti-GFP, & anti-Myc antibodies. Protein expression was analyzed by immunoblotting. (j) ChIP assays while using nuclear extracts of NUGC3 cells treated with siTEAD4. The ChIP-enriched DNA was subjected to PCR. (k) Target genes of VGLL1 & YAP. NUGC3 cells were transfected with pcDNA3, pcDNA3-myc-VGLL1, or pcDNA3-myc-YAP, & the mRNA expression levels were analyzed by RT-PCR. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31816819), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-308_rabbit-polyclonal-gfp-antibody-31020241616054.jpg)
![Immunocytochemistry/Immunofluorescence: Rabbit GFP pAb [NB600-308] - Analysis of GFP in human Hs746T gastric cancer cell line. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb600-308_rabbit-polyclonal-gfp-antibody-immunocytochemistry-immunofluorescence-2032025112942..png)
![Lane 1: HeLa cells. Lane 2: mock transfected HeLa cell lysate. Load: 35 ug per lane. Primary antibody: GFP antibody at 1 ug/ml for 1 h at room temperature. Secondary antibody: IRDye(R) 800 conjugated Donkey-a-Goat IgG [H&L] MX7 () secondary antibody at 1:2,500 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa, 33 kDa for GFP. Other band(s): none.](http://images.novusbio.com/fullsize/nb100-1770_goat-polyclonal-gfp-antibody-25520231310522.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Viral recombination of Oxtr in anterior DG hilar neurons impairs discrimination of social, but not non-social, stimuli. a Schematic illustrating viral injection & behavioral testing timeline. b Representative images of Cre & GFP virus infection in aDG. Scale bar, 200 μm. c Representative images & quantifications of cFos immunoreactivity in granule cell layer of aDG (GFP: n = 7, Cre: n = 4). Scale bar, 75 μm. d Behavioral schematic (top) & quantification (bottom) of single object exploration (GFP: n = 11, Cre: n = 6). e Behavioral schematic (top) & quantification (bottom) of novel objection recognition (GFP: n = 11, Cre: n = 6). Quantifications are displayed as Habituation (trials 1–3), Test (trial 4), & discrimination ratio (trial 4). f Behavioral schematic (top) & quantification (bottom) of social exploration test (GFP: n = 11, Cre: n = 6). g Behavioral schematic (top) & quantification (bottom) of social discrimination task (GFP: n = 11, Cre: n = 6). Quantifications are displayed as Habituation (trials 1–3), Test (trial 4), & discrimination ratio (trial 4). All data are displayed as mean ± SEM Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29222469), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1770_goat-polyclonal-gfp-antibody-310202415525264.jpg)
![Immunohistochemistry: GFP Antibody [NB100-1770] - Macrophages regulate dormancy in tumor cells.a Representative image of triple immunofluorescently stained in E0771-GFP primary tumor tissue for tumor cells, macrophages, & NR2F1. Green = GFP; Red = NR2F1; White = IBA-1; Blue = DAPI. White arrow shows a macrophage. The yellow arrow contact between an NR2F1-positive tumor cell & a macrophage. Mϕ=Macrophage. Scale bar=20 μm. b Quantification showing frequency of distances between NR2F1+ tumor cells to nearest macrophage in primary tumor. Data is normalized to frequency of distances between all DAPI+ nuclei to nearest TMEM. Bar = mean. Error bars = ±SEM. n = 34 fields of view (551 × 316 µm2) in 4 animals. For comparison between 0 & 200 µm bins a two-tailed Mann-Whitney test used (p < 0.0001). ****p < 0.0001. c Representative immunofluorescence images of NR2F1 expression in E0771-GFP tumor cells cultured alone, in direct contact w/ BAC1.2F5 macrophages, or in direct contact w/ HUVEC endothelial cells. White arrows show macrophages or endothelial cells in direct contact w/ a tumor cell. Green = GFP; Red = NR2F1; Blue = DAPI. TC = Tumor Cell. Mϕ = Macrophage. EC = Endothelial Cell. Scale bar = 15 μm. d Percentage of NR2F1-positive tumor cells from each group in C. TC alone: n = 777 cells in 9 independent experiments; TC+Mϕ; n = 226 cells in 6 independent experiments, TC+EC = n = 359 cells in 4 independent experiments. Bar = mean. Error bars = ±SEM. For TC vs. TC+Mϕ (p = 0.0039), & for TC vs. TC+EC (p = 1), a two-tailed Kruskal-Wallis test w/ Dunn’s multiple comparisons adjustment used. For TC+Mϕ vs. TC+EC (0.012), a two-tailed one-way ANOVA w/ Sidak’s multiple comparison adjustment used. *p < 0.05. **p < 0.01; ns = not significant. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/35110548), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1770_goat-polyclonal-gfp-antibody-31020241553380.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Viral recombination of Oxtr in aCA2/CA3distal impairs discrimination of social, but not non-social, stimuli. a Schematic illustrating viral injection & behavioral testing timeline. b Representative images of Cre & GFP virus infection in aCA2/CA3. Scale bar, 200 μm. c Behavioral schematic (top) & quantification (bottom) of single object exploration (GFP: n = 7, Cre: n = 10). d Behavioral schematic (top) & quantification (bottom) of novel objection recognition (GFP: n = 7, Cre: n = 10). Quantifications are displayed as Habituation (trials 1–3), Test (trial 4), & discrimination ratio (trial 4). e Behavioral schematic (top) & quantification (bottom) of social exploration test (GFP: n = 7, Cre: n = 10). f Behavioral schematic (top) & quantification (bottom) of social discrimination task (GFP: n = 7, Cre: n = 10). Quantifications are displayed as Habituation (trials 1–3), Test (trial 4), & discrimination ratio (trial 4). All data are displayed as mean ± SEM Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29222469), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1770_goat-polyclonal-gfp-antibody-31020241682115.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Transduction of mature retinal ganglion cells.Dissociated retinal ganglion cells (RGCs) isolated from adult rats (a–c), mice (d–f) & zebrafish (g–i) were transduced with baculovirus (bv) encoding fGFP (rat & mice) or DsRed (zebrafish). Representative pictures of vehicle- (-) & bv -treated cultures (a,d,g) visualize transduced RGCs (GFP & DsRed, respectively) that were either co-stained with the neuronal marker beta III-tubulin (Tub, red) at 2 days after transduction (d.a.t) for rat & mice RGCs (a,d) or identified by EGFP expression for zebrafish RGCs at 6 d.a.t. (g). The percentage of transduced RGCs was determined at 1, 2 & 3 days after transduction (d.a.t.) for rat & mice (b,e) & at 4 & 6 d.a.t. for zebrafish (h). Treatment effects compared to vehicle-treated controls: ***p < 0.001, **p < 0.01. Scale bars: 50 μm. RGC survival (c,f,i) was not affected by baculovirus application (bv) compared to vehicle-treated controls (-). (j) Delayed transduction of adult zebrafish RGCs with DsRed-bv after 4 days in culture. Scale bar: 50 μm. (k,l) Co-transduction of adult rat RGCs with fGFP-bv & DsRed-bv. The two viruses were added to retinal cultures simultaneously at half the concentration of single transductions. Representative pictures show co-transduced, fGFP- & DsRed-expressing RGCs (green & red, respectively) that were co-stained against the neuronal marker beta III-tubulin (white) at 2 d.a.t. Scale bar: 50 μm. (k). The percentage of transduced RGCs was determined at 2 & 3 d.a.t. (l). Non-significant difference in co-tranduction efficiency compared to single transductions. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep38928), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1770_goat-polyclonal-gfp-antibody-31020241612616.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Transduction of dorsal root ganglion neurons.Dissociated dorsal root ganglion neurons (DRG) isolated from postnatal (a–c) or adult (d–f) rats & adult mice (g–i) were transduced with fGFP-baculovirus (bv). Representative pictures of vehicle (-) & bv -treated cultures (a,d,g) show transduced, GFP-expressing neurons (green) that were co-stained with the neuronal marker beta III-tubulin (Tub, red) at 2 days after transduction (d.a.t.). The percentage of transduced, GFP-expressing DRG neurons was determined at 1, 2 & 3 d.a.t. (b,e,h), revealing similar transduction efficiencies of 80–90% in postnatal & adult DRG neurons. Cell survival in postnatal (c) & adult (f, i) DRG cultures was not affected by bv-application compared to vehicle-treated controls (-). Scale bars: 100 μm. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep38928), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1770_goat-polyclonal-gfp-antibody-310202415525262.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - hnRNPA3 knockout enhances DNA damage in DPR expressing HeLa cells. a Immunocytochemical detection of gamma H2AX foci in GFP-tagged DPR-expressing HeLa WT & A3KO HeLa cells. b Fold change of gamma H2AX foci in HeLa WT & A3KO cells expressing the indicated DPRs relative to HeLa WT cells with GFP expression. N = 47–127 cells from 2 biological replicates. c Immunocytochemical detection of pATM foci in GFP-tagged DPR-expressing HeLa WT & A3KO HeLa cells. d Fold change of pATM foci in HeLa WT & A3KO cells expressing the indicated DPRs relative to HeLa WT cells with GFP expression. N = 71–263 cells from 2 biological replicates. GFP EGFP transfected, GA EGFP-tagged poly-GA 175 repeats transfected, GR EGFP-tagged poly-GR 177 repeats transfected, PR: EGFP-tagged poly-PR 176 repeats transfected. All graphs are shown as mean ± SEM. *p < 0.05, **p < 0.01; one-way ANOVA & Tukey’s post-hoc test. Scale bar 10 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31642962), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1770_goat-polyclonal-gfp-antibody-310202415541948.jpg)
![Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - Staining of cultured hippocampal neuron from rat. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GFP-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1614-img0003.jpg "Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - Staining of cultured hippocampal neuron from rat. ICC/IF image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - HeLa cells transiently expressing GFP were visualized using GFP antibody (green). Nuclei were counterstained with DAPI. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GFP-Antibody-Chromatin-Immunoprecipitation-NB100-1614-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - IF analysis of GFP in mouse trigeminal ganglia, cornea. Image courtesy of product review by Jessica Newsom.](http://images.novusbio.com/fullsize/GFP-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1614-img0001.jpg)
![Immunohistochemistry: GFP Antibody [NB100-1614] - 5dpf Tg(brn3c:GFP) larvae were incubated with vehicle alone (DMSO, A), 400uM of cisplatin (CP, B), pre-treated with Qx for 2 hours and then incubated with CP for 6 hours (Qx, CP, C,E,G) or pre-treated with Qx for 2 hours and then co-treated with Qx and CP for 6 more hours (Qx+CP, D,F,H). Animals were fixed and immunostained for GFP (green) and otoferlin (red). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-33520-w), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GFP-Antibody-Immunohistochemistry-NB100-1614-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - IF analysis of GFP in rat cortical culture (2 weeks old). ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GFP-Antibody-Chromatin-Immunoprecipitation-NB100-1614-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - LNCaP human prostate cancer cell line imaged after transfection with GFP expressing plasmid. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GFP-Antibody-Immunocytochemistry-NB100-1614-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (RED).](http://images.novusbio.com/fullsize/GFP-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1614-img0010.jpg)
![Immunohistochemistry-Paraffin: GFP Antibody [NB100-1614] - Pyramidal neurons in the hippocampal formation of a neonatal mouse brain. Tissue was paraformaldehyde-fixed (4%) and paraffin-embedded. GFP staining is in green in left panel.](http://images.novusbio.com/fullsize/GFP-Antibody-Immunohistochemistry-Paraffin-NB100-1614-img0011.jpg)
![Immunohistochemistry-Paraffin: GFP Antibody [NB100-1614] - Embyonic mouse brain section. GFP in green, DAPI in grey. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GFP-Antibody-Immunohistochemistry-Paraffin-NB100-1614-img0016.jpg)
![Immunohistochemistry: GFP Antibody [NB100-1614] - (J) control. (K) Neo 200uM 30min. (L) GM 50uM 1 hour. (M) CP: 400uM 2 hours. (N) Qx 300uM 8 hours. (O) Qx 300uM 8 hours + Neo 200uM 30min. (P) Qx 300uM 8 hours + GM 50uM 1 hour. (Q) Qx 300uM 8 hours + CP 400uM 2 hours. Asterisks denote neuromast supporting cells positive for BrdU. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-33520-w), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GFP-Antibody-Immunohistochemistry-NB100-1614-img0017.jpg)
![Immunohistochemistry: Chicken Polyclonal GFP Antibody [NB100-1614] - Chicken Polyclonal GFP Antibody on mouse cancer tissue. DAPI (Red) and Cytopastmic GFP stain (Green). Primary antibody dilution: 1:200 in a 200 um slice. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-1614_chicken-polyclonal-gfp-antibody-immunohistochemistry-1610202310250..png)
![Immunohistochemistry-Frozen: Chicken Polyclonal GFP Antibody [NB100-1614] - Image depicting GFP in magenta and CD31 (Catalog # AF3628) in red. Tissue is a fixed frozen section of sciatic nerve obtained from a S100A4 GFP mouse. NB100-1614 was diluted 1 in 10000 and was left on tissue sections overnight at 4 degrees Celsius. Secondary antibody was donkey anti-chicken conjugated to Alexa 647. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-1614_chicken-polyclonal-gfp-antibody-immunohistochemistry-frozen-842024125355..jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1614] - Macrophages regulate dormancy in tumor cells.a Representative image of triple immunofluorescently stained in E0771-GFP primary tumor tissue for tumor cells, macrophages, & NR2F1. Green = GFP; Red = NR2F1; White = IBA-1; Blue = DAPI. White arrow shows a macrophage. The yellow arrow contact between an NR2F1-positive tumor cell & a macrophage. Mϕ=Macrophage. Scale bar=20 μm. b Quantification showing frequency of distances between NR2F1+ tumor cells to nearest macrophage in primary tumor. Data is normalized to frequency of distances between all DAPI+ nuclei to nearest TMEM. Bar = mean. Error bars = ±SEM. n = 34 fields of view (551 × 316 µm2) in 4 animals. For comparison between 0 & 200 µm bins a two-tailed Mann-Whitney test used (p < 0.0001). ****p < 0.0001. c Representative immunofluorescence images of NR2F1 expression in E0771-GFP tumor cells cultured alone, in direct contact w/ BAC1.2F5 macrophages,/in direct contact w/ HUVEC endothelial cells. White arrows show macrophages/endothelial cells in direct contact w/ a tumor cell. Green = GFP; Red = NR2F1; Blue = DAPI. TC = Tumor Cell. Mϕ = Macrophage. EC = Endothelial Cell. Scale bar = 15 μm. d Percentage of NR2F1-positive tumor cells from each group in C. TC alone: n = 777 cells in 9 independent experiments; TC+Mϕ; n = 226 cells in 6 independent experiments, TC+EC = n = 359 cells in 4 independent experiments. Bar = mean. Error bars = ±SEM. For TC vs. TC+Mϕ (p = 0.0039), & for TC vs. TC+EC (p = 1), a two-tailed Kruskal-Wallis test w/ Dunn’s multiple comparisons adjustment used. For TC+Mϕ vs. TC+EC (0.012), a two-tailed one-way ANOVA w/ Sidak’s multiple comparison adjustment used. *p < 0.05. **p < 0.01; ns = not significant. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/35110548), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1614_chicken-polyclonal-gfp-antibody-31020241554535.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1614] - Spontaneously metastasizing tumor cells survive significantly longer at the secondary site compared to intravenously injected tumor cells.a Representative intravital microscopy images showing the possible fates of extravascular disseminated tumor cells in the lung parenchyma. Top: Images of disseminated tumor cells just after extravasation. Bottom left: Example of an extravascular tumor cell, which has died, as evidenced by small extravascular apoptotic bodies (yellow arrow). Bottom middle: Example of an extravascular tumor cell that survived as a single & solitary tumor cell over time. Bottom right: Example of an extravascular tumor cell that began to divide & grow into a micro-metastasis. Red = tdTomato labeled endothelial cells & 155 kDa Tetramethylrhodamine dextran labeled blood serum, Green = GFP labeled tumor cells. Yellow dashed lines delineate blood vessel boundaries. Scale bar = 15 μm. b Percentage of extravascular E0771-GFP disseminated tumor cells that died, survived, or grew after extravasation in EM & SM models 64 hrs after arrival to the lung vasculature. EM: n = 27 tumor cells in 4 mice. SM: n = 31 tumor cells in 4 mice. Bar = mean. Error bars = ±SEM. For Died & Survived columns, a two-tailed unpaired t-test was used (p = 0.0003 & 0.0005, respectively). For Grew columns, a two-tailed Mann-Whitney test was used (p = 0.14). ***p < 0.001. ns = not significant. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35110548), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1614_chicken-polyclonal-gfp-antibody-31020241553510.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1614] - Qx protects against ototoxin-induced HC death & promotes supporting cell proliferation. (A–I) TUNEL assay (red) performed in zebrafish incubated w/ vehicle or 300 µM of Qx alone (controls) or w/ corresponding ototoxin w/ or w/out Qx 300 µM. Animals counterstained w/ phalloidin (green). (A) Neomycin (Neo) 200 µM incubation for 30 min. (B) Gentamicin (GM) 50 µM incubation for 1 hour. (C) Cisplatin (CP) 400 µM incubation for 2 hrs. (D) GM 50 µM incubation for 1 hour followed by recovery for 5 hrs. (E) Incubation w/ Qx 300 µM for 8 hrs & Neo 200 µM for 30 min. (F) Incubation w/ Qx 300 µM for 8 hrs & GM 50 µM for 1 hour. (G) Qx 300 µM for 8 hrs + CP 400 µM for 2 hrs. (H) Qx 300 µM incubation for 8 hrs + GM 50 µM for 1 hour followed by 5 hrs recovery. Asterisks denote TUNEL-positive HCs. (I) The % of TUNEL-positive neuromasts calculated for each treatment & represented as mean +/− SEM. (J–R) Proliferation assays performed in 5dpf Tg(brn3c:GFP) in presence/absence of Qx & corresponding ototoxin, by BrdU-labelling method (red). Animals immunostained for GFP (green). (J) control. (K) Neo 200 µM 30 min. (L) GM 50 µM 1 hour. (M) CP: 400 µM 2 hrs. (N) Qx 300 µM 8 hrs. (O) Qx 300 µM 8 hrs + Neo 200 µM 30 min. (P) Qx 300 µM 8 hrs + GM 50 µM 1 hour. (Q) Qx 300 µM 8 hrs + CP 400 µM 2 hrs. Asterisks denote neuromast supporting cells positive for BrdU. (R) The % of BrdU-positive supporting cells per neuromast calculated for each treatment & represented as mean +/− SEM. One-way ANOVA, *p < 0.05, **p < 0.01. Black asterisks compared versus corresponding control. Red asterisk compared versus corresponding ototoxin-only treatment. Scale bar: (A–H) 10 µm, (J–O) 7 μm. Data taken from at least 15 animals & 3 experiments runs. Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/30310154), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1614_chicken-polyclonal-gfp-antibody-3102024155524.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1614] - Spontaneously metastasizing tumor cells are more frequently doubly positive for dormancy & stem-like markers compared to intravenously injected tumor cells.a Representative images of triple immunofluorescence staining for GFP, NR2F1, & SOX9 expression in primary tumors, circulating tumor cells (CTCs), & disseminated tumor cells (Lung) from an E0771-GFP SM model (Left) & in disseminated tumor cells (Lung) from an EM model (Right). Green = GFP; Red = NR2F1; Orange = SOX9; Blue = DAPI. Scale bar for Primary Tumor = 50 μm. Scale bar for CTCs & Lung = 15 μm. b Percentage of double-positive tumor cells NR2F1-positive SOX9High from each group in Fig. 5a. Primary Tumor: n = 2383 in 97 fields of view (65 × 65 µm2) in 7 animals; CTCs: n = 379 cells in 8 animals; SM Lung: n = 104 cells in 9 animals; In vitro: n = 413 cells in 3 independent experiments. EM Lung: n = 75 cells in 7 animals. Bar = mean. Error bars = ±SEM. For EM Lung vs. SM Lung (p = 0.0001) & EM Lung vs. in vitro (p = 0.69), a two-tailed Kruskal-Wallis test with Dunn’s multiple comparisons adjustment was used. For PT vs. CTC: (p = 0.0041), PT vs. Lung SM (p = 0.0030), & CTC vs. Lung SM (p = 1.00) a two-tailed ANOVA test with Sidak’s multiple comparisons adjustment was used. **p < 0.01. ***p < 0.001. ns = not significant. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35110548), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1614_chicken-polyclonal-gfp-antibody-310202416571.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1614] - Dose protection curve against aminoglycosides. 5dpf larvae were incubated with vehicle (E3, A) or with 50 μM (D–F), 150 μM (G–I) or 300 μM (J–L) of Qx for a total of 8 hours. Gentamicin (GM, 50 μM, B,E,H,K) or neomycin (Neo, 200 µM,C,F,I,L) were added during the last 60 min or 30 min of incubation, respectively. Animals were fixed & stained for otoferlin (red) & GFP (green). (M) Quantification of the number of hair cells per neuromast after the different treatments represented as mean +/− SEM. Note that since no significant differences were found in the number of hair cells per neuromast when animals were incubated with the different Qx concentrations (0–300 µM, A,D,G,J), the control value represents the average of all these treatments. One-way ANOVA, Dunnett post test. *p < 0.05, **p < 0.01, ***p < 0.001. Black asterisks compared versus control. Red asterisks compared versus the corresponding aminoglycoside-only treatment. (N) Scores for neuromast morphology (see Materials & Methods). Scale bar: 7 μm. Data were taken from at least 20 animals & 3 experiments runs. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30310154), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1614_chicken-polyclonal-gfp-antibody-31020241555229.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1614] - Qx protects against cisplatin ototoxicity. 5dpf Tg(brn3c:GFP) larvae were incubated with vehicle alone (DMSO, A), 400 μM of cisplatin (CP, B), pre-treated with Qx for 2 hours & then incubated with CP for 6 hours (Qx, CP, C,E,G) or pre-treated with Qx for 2 hours & then co-treated with Qx & CP for 6 more hours (Qx + CP, D,F,H). Animals were fixed & immunostained for GFP (green) & otoferlin (red). (I) Quantification of the number of hair cells per neuromast after the different treatments represented as mean +/− SEM. One-way ANOVA, Dunnett post test. *p < 0.05, ***p < 0.001. Black asterisks compared versus control. Red asterisks compared versus CP 400 µM. (J) Scores for neuromast morphology (see Materials & Methods). Scale bar: 6 μm. Data were taken from at least 20 animals & 3 experiments runs. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30310154), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1614_chicken-polyclonal-gfp-antibody-31020241612686.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1614] - Dose protection curve against CP. 5dpf Tg(brn3c:GFP) larvae were incubated with 50 µM to 800 µM of CP (A,C,E,G) for 6 hours or pre-treated with 300 µM of Qx for 2 hours & then co-treated with Qx & CP (50µM-800µM) for 6 hours (B,D,F,H). Animals were fixed & immunostained for GFP (green) & otoferlin (red). Control animals were exposed to vehicle alone (DMSO). (I) Quantification of the number of hair cells per neuromast after the different treatments represented as mean +/− SEM. One-way ANOVA, Dunnett post test. ***p < 0.001. Black asterisks compared versus DMSO-treated animals. Red asterisks compared versus the corresponding CP concentration. (J) Scores for neuromast morphology (see Materials & Methods). Scale bar: 6 μm. Data were taken from at least 20 animals & 3 experiments runs. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30310154), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1614_chicken-polyclonal-gfp-antibody-310202415541991.jpg)
![Immunocytochemistry/Immunofluorescence: GFP Antibody (9F9.F9) [NB600-597] - GFP-transducted 293T cells. Image from verified customer review.](http://images.novusbio.com/fullsize/GFP-Antibody-9F9-F9-Immunocytochemistry-Immunofluorescence-NB600-597-img0019.jpg "Immunocytochemistry/Immunofluorescence: GFP Antibody (9F9.F9) [NB600-597] - GFP-transducted 293T cells. Image from verified customer review.")
![Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Lane 1: Molecular Weight Markers. Lane 2: GFP. Load: 10ng per lane. Primary antibody: Ms Anti-GFP antibody at 1:1000 for overnight at 4C. Secondary antibody: Mouse HRP secondary antibody at 1:40,000 for 30 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa, 27 kDa for epitope tag GFP. Other band(s): None.](http://images.novusbio.com/fullsize/GFP-Antibody-9F9-F9-Western-Blot-NB600-597-img0017.jpg)
![Western Blot: GFP Antibody (9F9.F9) [NB600-597] - All lanes have picogram amount recombinant GFP protein spiked into HeLa cell-derived lysates Lane 1: 64pg 2pg Lane 3: 16pg Lane 4: 8pg Lane 5: 4pg Lane 6: 2pg Lane 7: 1g Lane 8: 0pg. Primary antibody: anti-GFP monoclonal antibody at 1:400 for overnight at 4C. Secondary antibody: HRP-conjugated anti-Mouse IgG was performed at a dilution of 1:20,000 for 1h at 4C. Block: TTBS supplemented with 1% BSA for 1 h at 4C. Predicted/Observed size: 27 kDa for GFP. Other b&(s): none.](http://images.novusbio.com/fullsize/GFP-Antibody-9F9-F9-Western-Blot-NB600-597-img0015.jpg)
![Immunohistochemistry: GFP Antibody (9F9.F9) [NB600-597] - Used 1:5000 As referenced in: Lu S-M, Tremblay M-E` , King IL, Qi J, Reynolds HM, et al. (2011) HIV-1 Tat-Induced Microgliosis and Synaptic Damage via Interactions between Peripheral and Central Myeloid Cells. PLoS ONE 6(9): e23915. doi:10.1371/journal.pone.0023915 Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/GFP-Antibody-9F9-F9-Immunohistochemistry-NB600-597-img0020.jpg)
![Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Expression of GFP in CHO-K1 cells trasfected with GFP. Image from verified customer review.](http://images.novusbio.com/fullsize/GFP-Antibody-9F9-F9-Western-Blot-NB600-597-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GFP Antibody [NB600-303] - Analysis of FITC conjugate of NB600-303. Staining of mouse spleen cells Tissue: spleen cells infected with MHV68-H2bYFP. As seen in: Collins CM, Speck SH (2012) Tracking Murine Gammaherpesvirus 68 Infection of Germinal Center B Cells.](http://images.novusbio.com/fullsize/GFP-Antibody-Immunocytochemistry-Immunofluorescence-NB600-303-img0023.jpg "Immunocytochemistry/Immunofluorescence: GFP Antibody [NB600-303] - Analysis of FITC conjugate of NB600-303. Staining of mouse spleen cells Tissue: spleen cells infected with MHV68-H2bYFP. As seen in: Collins CM, Speck SH (2012) Tracking Murine Gammaherpesvirus 68 Infection of Germinal Center B Cells.")
![Western Blot: GFP Antibody [NB600-303] - Analysis using the FITC conjugate of NB600-303. Detection of Lane 1: GFP. Load: 50 ug per lane. Primary antibody: none. Secondary antibody: Fluorescein conjugated Anti-GFP at 1:1000 for 60 min at RT. Block: 1% BSA-TTBS for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa.](http://images.novusbio.com/fullsize/GFP-Antibody-Western-Blot-NB600-303-img0018.jpg)
![Western Blot: GFP Antibody [NB600-303] - WB analysis of GFP in various lysates. Image courtesy of product review by Emilie Leclerc.](http://images.novusbio.com/fullsize/GFP-Antibody-Western-Blot-NB600-303-img0005.jpg)
![Immunoprecipitation: GFP Antibody [NB600-303] - IP analysis of GFP in HeLa cell lysate. Image courtesy of anonymous customer review.](http://images.novusbio.com/fullsize/GFP-Antibody-Immunoprecipitation-NB600-303-img0007.jpg)
![Western Blot: GFP Antibody [NB600-303] - Analysis of GFP HRP expression in HeLa/GFP cell lysate.](http://images.novusbio.com/fullsize/GFP-Antibody-Western-Blot-NB600-303-img0014.jpg)
![Western Blot: GFP Antibody [NB600-303] - Analysis using Biotin conjugate of NB600-303. Lane 1: 50ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti- Biotin Conjugated secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blotting for 45 min at RT. HRP Streptavidin was used at 1:40,000 in incubated with blocking buffer for 30 min at 20C. Block: 5% Blotto 30 min at 20C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.](http://images.novusbio.com/fullsize/GFP-Antibody-Western-Blot-NB600-303-img0019.jpg)
![Immunohistochemistry: GFP Antibody [NB600-303] - Eight-week-old Lgr5-GFP mice were injected with CCL4 and rHGF or/and rRspo1 proteins (0.1 ug/mice/time), as indicated in a, Lgr5 expression was measured using an immunofluorescent assay with anti-GFP antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-01341-6) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GFP-Antibody-Immunohistochemistry-NB600-303-img0024.jpg)
![Western Blot: GFP Antibody [NB600-303] - Lane 1: 293FT cells transfected with CDK4 dominant negative. Lane 2: 293FT cells poitive control. Load: 25 ug per lane. Primary antibody: GFP antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa for GFP.](http://images.novusbio.com/fullsize/GFP-Antibody-Western-Blot-NB600-303-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GFP Antibody [NB600-303]](http://images.novusbio.com/fullsize/GFP-Antibody-Immunocytochemistry-Immunofluorescence-NB600-303-img0022.jpg)
![Western Blot of Rabbit anti-GFP antibody. Lane 1: Wild type GFP (0.1 ug) was used to spike HeLa whole cell lysate. Lane 2: none. Load: 30 ug per lane. Primary antibody: GFP antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) Goat-a-Rabbit IgG [H&L] MX10 () at 1:10,000 for 45 min at RT. Block: 5% BLOTTO in PBS overnight at 4C. Predicted/Observed size: 27 kDa for epitope tag GFP. Other band(s): none.](http://images.novusbio.com/fullsize/nb600-303_rabbit-polyclonal-gfp-antibody-24520231052434.jpg)
![Western Blot: GFP Antibody [NB600-303] - The polo-boxes of Plk4 are not sufficient to mediate STIL binding.(A) Scheme of Flag-Plk4 fragments. (B) After overexpression of Flag-Plk4-fragments (A) & GFP-STIL in HEK293T cells, cell lysates were subjected to immunoprecipitations using anti-Flag antibodies. Coprecipitation of GFP-STIL with Flag-Plk4 fragments was detected by western blotting using anti-GFP & anti-Flag antibodies. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/4/3/370/1390/Plk4-dependent-phosphorylation-of-STIL-is-required), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-303_rabbit-polyclonal-gfp-antibody-31020241529192.jpg)
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-303] - Lgr5+ liver stem cells transplantation decreased liver fibrosis. a Schematic overview of the experimental setup. Eight-week-old wild-type C57 mice were i.p. injected with CCL4 (2 ml/kg, Sigma-Aldrich) dissolved in olive oil at a ratio of 1:4, or olive oil alone (2 ml/kg) twice a week for 6 weeks. Lgr5-GFP+ liver stem cells or primary hepatocyte (PH) derived from Lgr5-GFP mice were transplanted into the liver fibrosis mice by intrasplenical injection on day 0. b Lgr5+ cells were isolated from Lgr5-GFP mice treated with CCL4 by FACS assay for transplantation (left). The liver Lgr5 expression was stained using anti-GFP antibody in mice with Lgr5+ cells or PH transplantation (right) on day 40. n = 10 mice, scale bars, 200 μm. c, d Lgr5+ liver stem cells transplantation decreased CCL4-induced liver fibrosis & recovered liver functions. Lgr5-GFP+ liver stem cells or PH transplantation were described in a; the livers were harvested & stained using H&E & Sirius Red for fibrosis analysis & quantification of positive-staining areas measured by Image J software (c). The serum was harvested for ALT & AST analysis (d, e). For c, scale bars, 200 μm, the results are shown as mean ± s.d. of five independent sections taken randomly per mice & a total of 50 tissue specimens in each group (n = 10 mice) *p < 0.05, **p < 0.01. For d, e, triplicates for each condition were analyzed. The results are shown as mean ± s.d. of three independent experiments. *p < 0.05, **p < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29079780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-303_rabbit-polyclonal-gfp-antibody-31020241616099.jpg)
![Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Expression of GFP in HEK293T cells. This image was submitted via customer Review. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/GFP-Antibody-4B10B2-Western-Blot-NBP2-22111-img0005.jpg "Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Expression of GFP in HEK293T cells. This image was submitted via customer Review. Image using the HRP form of this antibody.")
![Immunocytochemistry/Immunofluorescence: GFP Antibody (4B10B2) [NBP2-22111] - Analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP mouse mAb .](http://images.novusbio.com/fullsize/GFP-Antibody-4B10B2-Immunocytochemistry-Immunofluorescence-NBP2-22111-img0004.jpg)
![Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Analysis using GFP mouse mAb against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).](http://images.novusbio.com/fullsize/GFP-Antibody-4B10B2-Western-Blot-NBP2-22111-img0001.jpg)
![Flow Cytometry: GFP Antibody (4B10B2) [NBP2-22111] - Analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP mouse mAb .](http://images.novusbio.com/fullsize/GFP-Antibody-4B10B2-Flow-Cytometry-NBP2-22111-img0003.jpg)
![Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Analysis using GFP mouse mAb against extracts from HCC827 cells, untransfected (1) and transfected with GFP (2).](http://images.novusbio.com/fullsize/GFP-Antibody-4B10B2-Western-Blot-NBP2-22111-img0002.jpg)
![Western Blot: GFP Antibody (859) [NBP2-43575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (5 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with GFP antibody (859) diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GFP-Antibody-859-Western-Blot-NBP2-43575-img0007.jpg "Western Blot: GFP Antibody (859) [NBP2-43575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (5 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with GFP antibody (859) diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GFP Antibody (859) [NBP2-43575] - GFP-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: GFP stained by GFP antibody [859] diluted at 1:2000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/GFP-Antibody-859-Immunocytochemistry-Immunofluorescence-NBP2-43575-img0006.jpg)
![Western Blot: GFP Antibody (859) [NBP2-43575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GFP antibody [859] diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GFP-Antibody-859-Western-Blot-NBP2-43575-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: GFP Antibody (859) [NBP2-43575] - Analysis of GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFP is expressed in the tranfected cell Red: .GFP-recombinant protein stained by GFP-tag antibody diluted at 1:2000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/GFP-Antibody-859-Immunocytochemistry-Immunofluorescence-NBP2-43575-img0002.jpg)
![ELISA: GFP Antibody (859) [NBP2-43575] - Coating Ab: mouse anti-GFP (2ug/well) Sample: GFP protein Detected Ab: rabbit anti-GFP Conjugated Ab: goat anti-rabbit IgG-HRP (1:20000)](http://images.novusbio.com/fullsize/GFP-Antibody-859-ELISA-NBP2-43575-img0001.jpg)
![Immunoprecipitation: GFP Antibody (859) [NBP2-43575] - Analysis of 400 ug cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 ug of GFP tag antibody 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/GFP-Antibody-859-Immunoprecipitation-NBP2-43575-img0003.jpg)
![Simple Western: GFP Antibody [Unconjugated] [AF4240] - Design for a membrane-localized ACE2 expression system. (A) Our ACE2 construct is driven by a CMV promoter followed by the first 25 residues of ACE2 containing the leader sequence that direct ACE2 to the plasma membrane. This is followed by a 3xHA tag linked to the remainder of ACE2 (20-805) and a C-terminal sfGFP. Both 3xHA and sfGFP fusions are separated from ACE2 by flexible 3xGGGGS linkers. (B) The ACE2 fusion protein is designed to be embedded in the plasma membrane where it can perform extracellular carboxypeptidase-mediated metabolism and its levels can be detected by cell staining with antibodies to HA. (C) Lysates from untransfected or 3xHA-ACE2-sfGFP-transfected HEK293 cells were analyzed by automated Jess capillary immunoassay using antibodies to HA, GFP, and two ACE2 antibodies. (D) Confocal fluorescence microscopy of HEK cells transfected with 3xHA-ACE2-sfGFP and stained with HA and the nuclear stain DAPI. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37644110), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af4240_gfp-affinity-purified-polyclonal-ab-30820241044553.jpg)
![Western Blot: eGFP Antibody [NBP2-37821] - WB analysis on HEK293 cells transfected with Green Fluorescent Protein (GFP) using eGFP antibody. 20ug whole cells lyate were loaded. Image from verified customer review.](http://images.novusbio.com/fullsize/eGFP-Antibody-Western-Blot-NBP2-37821-img0004.jpg "Western Blot: eGFP Antibody [NBP2-37821] - WB analysis on HEK293 cells transfected with Green Fluorescent Protein (GFP) using eGFP antibody. 20ug whole cells lyate were loaded. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: eGFP Antibody [NBP2-37821] - Analysis of eGFP using either natural fluorescence (green) or an eGFP antibody (red) in U2OS cells transfected with an EGFR-eGFP fusion protein.](http://images.novusbio.com/fullsize/eGFP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-37821-img0003.jpg)
![Western Blot: eGFP Antibody [NBP2-37821] - 12% SDS-PAGE of HEK-293 cells transfected with pCMV-Myc-eGFP (0.25ug DNA lanes 2 and 4; 0.50ug DNA lanes 3 and 5) Coomassie stain (lanes 2 and 3) and western blot (lanes 4 and 5) with anti-eGFP antibody and goat anti-rabbit HRP secondary](http://images.novusbio.com/fullsize/eGFP-Antibody-Western-Blot-NBP2-37821-img0001.jpg)
