GFP Antibody

Images

 
Western Blot: GFP Antibody [NB100-1614] - WB analysis of GFP expression in HeLa/GFP cell lysate
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1614] - Staining of cultured hippocampal neuron from rat. Image provided via product review by verified customer.
Chromatin Immunoprecipitation: GFP Antibody [NB100-1614] - HeLa cells transiently expressing GFP were visualized using GFP antibody (green). Nuclei were counterstained with DAPI. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1614] - IF analysis of GFP in mouse trigeminal ganglia, cornea. Image courtesy of product review by Jessica Newsom.
Immunocytochemistry: GFP Antibody [NB100-1614] - LNCaP human prostate cancer cell line imaged after transfection with GFP expressing plasmid. Image from verified customer review.
Chromatin Immunoprecipitation: GFP Antibody [NB100-1614] - IF analysis of GFP in rat cortical culture (2 weeks old). Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1614]

Product Details

Summary
Reactivity JfSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
10.0 mg/ml

Order Details

GFP Antibody Summary

Immunogen
Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in adjuvant. After multiple injections, eggs were collected from The hens, and IgY fractions were prepared from The yolks. Purified antibody preparations were Then mixed with glycerol 1:1(v/v) (toprevent freezing at -20 degrees C), augmented with thimerosal, and then filter-sterilized.
Specificity
GFP
Clonality
Polyclonal
Host
Chicken
Gene
GFP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2500-1:5000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunocytochemistry
Application Notes
Use in IHC-F was reported in the scientific literature (PMID: 23799397).
Reviewed Applications
Read 5 Reviews rated 4.4
using
NB100-1614 in the following applications:

Publications
Read Publications using
NB100-1614 in the following applications:

Reactivity Notes

NB 100-1614 has been tested on transgenic mice expressing recombinant GFP. Jellyfish reactivity reported in scientific literature (PMID: 27364165). This is a recombinantly expressed GFP protein used in Mouse (PMID: 29110094).

Packaging, Storage & Formulations

Storage
Store at -20C in the dark. Avoid freeze-thaw cycles.
Buffer
10mM PBS (0.9% isotonic, w/v, pH 7.2) and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
10.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GFP Antibody

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB100-1614)(24)

We have publications tested in 6 confirmed species: Mouse, Drosophila, Firefly, Jellyfish, Primate, Yeast.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, IHC-P.


Filter By Application
ICC/IF
(7)
IHC
(8)
IHC-Fr
(4)
IHC-P
(2)
All Applications
Filter By Species
Mouse
(10)
Drosophila
(1)
Firefly
(1)
Jellyfish
(1)
Primate
(1)
Yeast
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB100-1614 Applications Species
Lean GA, Liu YJ, Lyon DC. Cell type specific tracing of the subcortical input to primary visual cortex from the basal forebrain. J. Comp. Neurol. 2018 Feb 14 [PMID: 29441578] (IHC) IHC
Xuan X, Gao F, Ma X et al. Activation of ACE2/angiotensin (1-7) attenuates pancreatic B cell dedifferentiation in a high-fat-diet mouse model. Metab. Clin. Exp. 2017 Dec 07 [PMID: 29225087] (Mouse) Mouse
Ehrlich AT, Semache M, Bailly J et al. Mapping GPR88-Venus illuminates a novel role for GPR88 in sensory processing Brain Struct Funct. 2017 Nov 06 [PMID: 29110094] (Mouse) Mouse
Ogawa Y, Kanda T, Vogt K, Yanagisawa, M. Anatomical and Electrophysiological Development of the Hypothalamic Orexin Neurons from Embryos to Neonates. J. Comp. Neurol. 2017 Jun 12 [PMID: 28608460]
Choi J, Clement K, Huebner AJ et al. DUSP9 Modulates DNA Hypomethylation in Female Mouse Pluripotent Stem Cells. Cell Stem Cell. Mar 29 2017 [PMID: 28366588]
Raza QS, Vanderploeg JL, Jacobs JR. Matrix Metalloproteinases are required for membrane motility and lumenogenesis during Drosophila heart development. PLoS ONE. 2017 Feb 13 [PMID: 28192468] (IHC, Drosophila) IHC Drosophila
Mullen BR, Ross B, Chou JW et al. A Complex Interaction Between Reduced Reelin Expression and Prenatal Organophosphate Exposure Alters Neuronal Cell Morphology ASN Neuro 2016 Jun 01 [PMID: 27364165] (ICC/IF, Jellyfish) ICC/IF Jellyfish
Feng L, Wang Y, Cai H et al. ADAM10-Notch signaling governs the recruitment of ovarian pregranulosa cells and controls folliculogenesis in mice. J. Cell. Sci. 2016 Apr 15 [PMID: 27084580] (IHC-P) IHC-P
Klein C, Rasinska J, Empl L et al. Physical exercise counteracts MPTP-induced changes in neural precursor cell proliferation in the hippocampus and restores spatial learning but not memory performance in the water maze. Behav Brain Res 2016 Jul 1 [PMID: 27012392]
Sorrentino NC, Maffia V, Strollo S et al. A Comprehensive Map of CNS Transduction by Eight Recombinant Adeno-associated Virus Serotypes Upon Cerebrospinal Fluid Administration in Pigs. Mol. Ther. 2015 Dec 07 [PMID: 26639405] (IHC-Fr) IHC-Fr
Show All 24 Publications.

Reviews for GFP Antibody (NB100-1614) (5) 4.45

Average Rating: 4.4
(Based on 5 reviews)
We have 5 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB100-1614:
Filter by Applications
ICC
(2)
IF
(2)
IHC
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
Rat
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry GFP NB100-1614
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reviewed by:
Anonymous
ICC Human 05/09/2018
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Summary

ApplicationImmunocytochemistry
Sample TestedLNCaP human prostate cancer cell line
SpeciesHuman
LotB-2

Comments

CommentsHuman LnCap cells were grown in RPMI1640 + 10% FCS in 5% CO2 at 37C. The cells were electroporated with GFP expressing plasmid using Amaxa Nucleofection kit.
Immunocytochemistry GFP NB100-1614
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reviewed by:
Anonymous
ICC Human 12/25/2016
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Summary

ApplicationImmunocytochemistry
Sample Tested hela cell
SpeciesHuman

Comments

CommentsGFP expressing cells were fixed with PFA 4% for 30 min, washed several times with PBS and then permeabilized for 5 min with PBSTx-100 1%. After several washes cells were incubated with the chicken anti-GFP 1:500 in fish blocking solution (PBS + 2.5% fish gelatin + 2.5% FCS) overnight at 4C. The next day after several washes with PBS, cells were incubated with anti-chicken Alexa 488 1:400 in fish gelatin for 1hour at room temperature.
Immunofluorescence GFP NB100-1614
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reviewed by:
Anonymous
IF Rat 02/17/2016
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Summary

ApplicationImmunofluorescence
Sample Testedrat cortical culture (2 weeks old)
SpeciesRat

Blocking

Blocking Details4% BSA, 4%goat serum, 0.1%TritonX-100 in TBS. Blocking for 1hr at 4C

Primary Anitbody

Dilution Ratio1:500 for surface GFP labeling, 1:1000 for total GFP labeling

Secondary Antibody

Secondary Descriptiongoat anti-chicken Alexa fluor 488, goat anti-chicken Alexa fluor 568
Secondary Manufacturer Cat#ThermoScientific: A-11039, A-11041
Secondary Concentration0.4mg/mL (1:500 dilution)

Details

Detection NotesZeiss confocal microscopy 710 (63x oil objective, pixel time 0.64us )
Fixation DetailsLive neurons were fed with GFP antibody in culture media for 10min at 37C before fixation in fixation buffer(4%PFA,4%sucrose TBS
Wash DescriptionTBS. Wash 3 times (5min each) between each steps.

Comments

CommentsI aimed to perform both surface and total GFP-GluA2 labeling in neurons. Here is a brief summary of the protocal. For surface labeling, the live neurons were fed with GFP antibodies for 10min followed by fixation with 4% PFA for 15min. Then sample were blocked in non-permeable blocking buffer (without TritonX-100)for 1hr, followed by goat anti chicken Alexa Fluor 488. Then the sample were blocked in permeable blocking buffer (with TritonX-100) for 1hr or overnight before incubation with GFP primary antibody followed by goat anti-chicken Alexa fluor 568. After final wash, the samples were mounted to glass slide with ProLongĀ® Diamond Antifade Mountant (Thermoscitific P36961).
Immunofluorescence GFP NB100-1614
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reviewed by:
Hongtian Yang
IF Rat 08/23/2013
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Summary

ApplicationImmunofluorescence
Sample Testedcultured hippocampal neuron from rat
SpeciesRat
Lot0511FP12
CommentsThe chicken GFP antibody worked very well. The reason we choose chicken the version of Novus' GFP antibody is because it allows us to do double or triple staining if necessary, since most other primary antibody's are usually from rabbit or mouse.

Blocking

Blocking Details0.1% TritonX-100, 5% goat serum in PBS; 1hr, 4C

Primary Anitbody

Dilution Ratio1:800, overnight, 4C

Secondary Antibody

Secondary Descriptiongoat anti-chicken IgG (H+L), Alexa 488
Secondary Manufacturer Cat#A11039
Secondary Concentration4ug/ml

Details

Detection NotesZeiss Confocal Microscopy
Fixation Details4% formaldehyde, PBS, room temperture
Wash DescriptionPBS, 10min, 3 times per solution change

Comments

CommentsThe chicken GFP antibody worked very well. The reason we choose chicken the version of Novus' GFP antibody is because it allows us to do double or triple staining if necessary, since most other primary antibody's are usually from rabbit or mouse.
Immunohistochemistry GFP NB100-1614
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reviewed by:
Anonymous
IHC Mouse 05/15/2012
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Summary

ApplicationImmunohistochemistry
Sample TestedMouse Trigeminal Ganglia, Cornea
SpeciesMouse
Lot0316FP11
CommentsUsed 1:40 dilution of primary at 1:8000

Blocking

Blocking Details5% NGS

Primary Anitbody

Dilution Ratio1:40 dilution in NGS/PBS, overnight incubation at room temperature

Secondary Antibody

Secondary DescriptionGoat anti-chicken
Secondary Manufacturer Cat#A11039
Secondary Concentration1:10 dilution in 10% NGS, used at 1:1500

Details

Detection NotesCut tissue via cryostat, viewed on Nikon E800 microscope at 10, 20x. Exposure time was negligible. Only negative controls used
Fixation Details20um sections of formalin-fixed tissue
Wash DescriptionWash 3x for 10min each in 1%NGS

Comments

CommentsUsed 1:40 dilution of primary at 1:8000

Product General Protocols

View specific protocols for GFP Antibody (NB100-1614): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for GFP Antibody (NB100-1614). (Showing 1 - of FAQs).

    Secondary Antibodies

     

    Isotype Controls

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    Bioinformatics Tool for GFP Antibody (NB100-1614)

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    Blogs on GFP.

    The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
    GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.Ā  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

    GFP - Be Green!
    Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

    GFP (Green Fluorescent Protein) Bringing Light to Life
    GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

    BDNF Antibodies Aid Research on Alzheimer's Therapies
    Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

    Epitope Tags and the V5 Tag Antibody
    We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

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    Recent Reviews

    4.4
    5
    2
    4
    3
    3
    0
    2
    0
    1
    0

    Anonymous
    05/09/2018
    Application: ICC
    Species: Human

    Anonymous
    12/25/2016
    Application: ICC
    Species: Human

    Anonymous
    02/17/2016
    Application: IF
    Species: Rat

    Bioinformatics

    Gene Symbol GFP