GFP Antibody [Biotin]

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Product Details

Summary
Reactivity JfSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

Order Details

GFP Antibody [Biotin] Summary

Immunogen
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Anti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). Primate reactivity reported in scientific literature (PMID: 27723741).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GFP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1:20000 - 1:120000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB600-308B in the following applications:

Reactivity Notes

No reaction was observed against Human, Mouse serum proteins. Suitable for detecting fusion proteins containing the GFP sequence expressed in Human, Mouse, Rat, C. elegans, Drosophila and in vitro transcription/translation systems and transgenic animals. GFP Transgenic Rat reactivity reported in scientific literature (PMID:25724725). Transgenic C. elegans reactivity reported in scientific literature (PMID: 27110099). Known cross reactivity with wt and all variants such as rGFP, eGFP, S65T-GFP, RS-GFP, YFP and EGFP.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GFP Antibody [Biotin]

  • anti-gfp (green fluorescent protein) pab
  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP immunofluorescence
  • GFP immunoprecipitation
  • GFP mouse
  • GFP staining
  • GFP western blot
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

This GFP antibody has been developed specifically for use in applications requiring a high titer and specificity with minimum background. Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB600-308B) (0)

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Review for GFP Antibody (NB600-308B) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB600-308B:
Filter by Applications
IHC-Fr
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen GFP NB600-308B
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reviewed by:
Anonymous
IHC-Fr Mouse 07/13/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested Mouse brain
SpeciesMouse
Lot33459-050818-B

Comments

CommentsDilution 1:200

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFP Antibody (NB600-308B). (Showing 1 - of FAQ).

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    Blogs on GFP.

    The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
    GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

    GFP - Be Green!
    Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

    GFP (Green Fluorescent Protein) Bringing Light to Life
    GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

    BDNF Antibodies Aid Research on Alzheimer's Therapies
    Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

    Epitope Tags and the V5 Tag Antibody
    We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

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    Recent Reviews

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    Anonymous
    07/13/2018
    Application: IHC-Fr
    Species: Mouse

    Bioinformatics

    Gene Symbol GFP