GFP Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Analysis using the Biotin conjugate of NB100-1770. Staining of transgenic mouse pancreas, expressing GFP in beta cells. Image courtesy of product ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Analysis of Biotin conjugate of NB100-1770. Tissue: Drosophila melanogaster late stage embryonic central nervous system. Fixation: 0.5% PFA. Antigen ...read more
Western Blot: GFP Antibody [NB100-1770] - Analysis using the FITC conjugate of NB100-1770. Lane 1: GFP (50 ug). Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1000 for 60 min at RT. ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - HEK293 cells stable transfected with integrin alpha8-GFP, were immunostained for GFP (green) and counterstained with phalloidin (red) and DAPI (BLUE). ...read more
Western Blot: GFP Antibody [NB100-1770] - Analysis using the Alkaline Phosphatase conjugate of NB100-1770. Lane 1: GFP (50 ng). Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Predicted/Observed ...read more
Western Blot: GFP Antibody [NB100-1770] - 9 Poly-GA co-immuno - precipitation with pATM. Aggregated poly-GA selectively co-immunoprecipitates with pATM. Image collected and cropped by CiteAb from the following ...read more
Immunohistochemistry: GFP Antibody [NB100-1770] - E5.5 Hex-GFP transgenic mouse embryo tissue. Primary antibody: Goat anti-GFP at 1:500 dilution. Secondary antibody: Fluorochrome conjugated Anti-goat IgG antibody at ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - IF analysis of GFP in mouse brain. Image courtesy of product review by Tatyana Pivneva.
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS o/n, and subsequently ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Analysis of DyLight 488 conjugate of NB100-1770. Human breast carcinoma tissue. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: ...read more
Immunohistochemistry: GFP Antibody [NB100-1770] - GFP-positive transplanted NPCs have morphological features of hippocampal pyramidal neurons at day 90 after grafting. IHC image submitted by a verified customer review.

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, ELISA, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, IHC-FrFl, KD, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.1 mg/ml

Order Details

GFP Antibody Summary

Immunogen
This GFP antibody was developed against a fusion protein corresponding to the full length GFP (246aa) derived from the jellyfish Aequorea victoria.
Specificity
GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:400 - 1:2000
  • Electron Microscopy 1:10 - 1:500
  • ELISA 1:40000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Proximity Ligation Assay
  • Immunohistochemistry Free-Floating 1:10 - 1:500
  • Knockdown Validated
  • Knockout Validated
Reviewed Applications
Read 6 Reviews rated 4.8
using
NB100-1770 in the following applications:

Publications
Read Publications using
NB100-1770 in the following applications:

  • 1 publication
  • EM
    2 publications
  • 3 publications
  • 25 publications
  • IF
    1 publication
  • IHC
    20 publications
  • 12 publications
  • 1 publication
  • 8 publications
  • KD
    1 publication
  • KO
    1 publication
  • PLA
    1 publication
  • WB
    3 publications

Reactivity Notes

No reaction was observed against Human, or Rat serum proteins. Known Cross Reactivity: rGFP. YFP differs from GFP due to a mutation at Thr203Tyr; antibodies raised against full-length GFP should also detect YFP and other variants. Reactivity in transgenic mice with GFP. Reactivity in human cell lines transfected will a GFP construct.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GFP Antibody

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T.,... Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S.,... Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB100-1770)(91)

We have publications tested in 4 confirmed species: Human, Mouse, Jellyfish, Non-species specific.

We have publications tested in 13 applications: ELISA, EM, Flow, ICC/IF, IF, IHC, IHC-Fr, IHC-FrFl, IHC-P, KD, KO, PLA, WB.


Filter By Application
ELISA
(1)
EM
(2)
Flow
(3)
ICC/IF
(25)
IF
(1)
IHC
(20)
IHC-Fr
(12)
IHC-FrFl
(1)
IHC-P
(8)
KD
(1)
KO
(1)
PLA
(1)
WB
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(6)
Jellyfish
(1)
Non-species specific
(2)
All Species
Showing Publications 1 - 10 of 91. Show All 91 Publications.
Publications using NB100-1770 Applications Species
Wei H, Wu X, You Y et al. Systematic analysis of purified astrocytes after SCI unveils Zeb2os function during astrogliosis Cell reports Feb 2 2021 [PMID: 33535036] (IHC, ICC/IF, Non-species specific) IHC, ICC/IF Non-species specific
Ping S, Qiu X, Gonzalez-Toledo ME, et al. Stem Cell Factor in Combination With Granulocyte Colony-Stimulating Factor Protects the Brain From Capillary Thrombosis-Induced Ischemic Neuron Loss in a Mouse Model of CADASIL Frontiers in cell and developmental biology Jan 12 2020 [PMID: 33511138] (IHC) IHC
Brandes N, Mitkovska SH, Botermann DS et al. Spreading of Isolated Ptch Mutant Basal Cell Carcinoma Precursors Is Physiologically Suppressed and Counteracts Tumor Formation in Mice International journal of molecular sciences Dec 5 2020 [PMID: 33291515] (ICC/IF, Non-species specific) ICC/IF Non-species specific
Campbell BC, Nabel EM, Murdock MH et al. mGreenLantern: a bright monomeric fluorescent protein with rapid expression and cell filling properties for neuronal imaging Proc Natl Acad Sci U S A Nov 18 2020 [PMID: 33208539] (ICC/IF)

Details:
HEK293T cells transfected with pcDNA3.1-[FP] plasmid underwent immunocytochemistry and immunofluorescence analysis.
ICC/IF
Stone D, Kenkel EJ, Loprieno MA et al. Gene Transfer in Adeno-Associated Virus Seropositive Rhesus Macaques Following Rapamycin Treatment and Subcutaneous Delivery of AAV6, but Not Retargeted AAV6 Vectors Hum Gene Ther Nov 2 2020 [PMID: 32998579]
May PJ, Gamlin PD. Is Primate Lens Accommodation Unilaterally or Bilaterally Controlled? Invest Ophthalmol Vis Sci Jul 8 2020 [PMID: 32634204] (ICC/IF) ICC/IF
Rose M, Gao K, Cortez-Toledo E et al. Endothelial cells derived from patients' induced pluripotent stem cells for sustained factor VIII delivery and the treatment of hemophilia A Stem Cells Transl Med Mar 13 2020 [PMID: 32162786] (IHC-Fr) IHC-Fr
Neves J, Chirco KR, Cedron-Craft W et al. MANF delivery improves retinal homeostasis and cell replacement therapies in ageing mice Exp Gerontol Mar 3 2020 [PMID: 32119994] (IHC) IHC
Sethi NS, Kikuchi O, Duronio GN et al. Early TP53 alterations engage environmental exposures to promote gastric premalignancy in an integrative mouse model Nat Genet Feb 7 2020 [PMID: 32025000] (IHC-P) IHC-P
Li X, Feng Y, Yan M et al. Inhibition of Autism-Related Crm1 Disrupts Mitosis and Induces Apoptosis of the Cortical Neural Progenitors Cereb. Cortex Feb 1 2020 [PMID: 32008040] (ICC/IF) ICC/IF
Show All 91 Publications.

Reviews for GFP Antibody (NB100-1770) (6) 4.86

Average Rating: 4.8
(Based on 6 reviews)
We have 6 reviews tested in 2 species: Mouse, cells.

Reviews using NB100-1770:
Filter by Applications
WB
(1)
IF
(2)
ICC
(1)
IHC
(2)
All Applications
Filter by Species
Mouse
(5)
cells
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot GFP NB100-1770
Enlarge
5
reviewed by:
Anonymous
WB Mouse 03/08/2020
View

Summary

ApplicationWestern Blot
Sample Tested4T1 mouse breast cancer cell line
SpeciesMouse
Lot35578
Immunofluorescence GFP NB100-1770
Enlarge
4
reviewed by:
Anonymous
IF Mouse 10/21/2019
View

Summary

ApplicationImmunofluorescence
Sample TestedTumor sections
SpeciesMouse
Lot35447
Immunocytochemistry GFP NB100-1770
Enlarge
5
reviewed by:
Anonymous
ICC cells 05/08/2018
View

Summary

ApplicationImmunocytochemistry
Sample TestedItga8-GFP transfected HEK293 cells
Speciescells
LotNA
  5
reviewed by:
Anonymous
IF Mouse 12/12/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24753232
SpeciesMouse

Blocking

Blocking DetailsSee PMID 24753232

Details

Detection NotesSee PMID 24753232

Comments

CommentsPublished in PMID: 24753232
Immunohistochemistry GFP NB100-1770
Enlarge
5
reviewed by:
Anonymous
IHC Mouse 06/19/2014
View

Summary

ApplicationImmunohistochemistry
SpeciesMouse
Immunohistochemistry GFP NB100-1770
Enlarge
5
reviewed by:
Anonymous
IHC Mouse 05/15/2012
View

Summary

ApplicationImmunohistochemistry
Sample Tested Mouse brain
SpeciesMouse
Lot25297

Blocking

Blocking Details2% BSA and 0.3% Triton X-100 for 2 hours at RT

Primary Anitbody

Dilution Ratiodilution 1:5000 for 24 hours at 4 C

Secondary Antibody

Secondary Descriptiondonkey anti-goat Alexa 488
Secondary Concentration1:1000

Details

Fixation Detailsfixation by 4% paraformaldehyde, permeabilization by 0.3% Triton X-100
Wash Descriptionwashing by PBS

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFP Antibody (NB100-1770). (Showing 1 - 1 of 1 FAQs).

  1. Does this antibody NB100-1770 detect eGFP?
    • NB100-1770 has not yet been tested against eGFP by our lab. Theoretically, you should be able to detect the eGFP with this antibody, as the two are very homologous; however, we cannot guarantee it. If you would like to test an antibody in an untested species and/or application and share your results with us, I can recommend our Innovator's Reward program. Our Innovator's Reward program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Reviews may also be submitted by emailing Innovators Reward.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alkaline Phosphatase NB600-1502
Biotin NB100-1678
DyLight 488 NBP1-69969
FITC NB100-1771
FITC NB100-1771
HRP NB600-313
Rhodamine NBP1-97327
Texas Red NB120-6660

Additional GFP Products

Research Areas for GFP Antibody (NB100-1770)

Find related products by research area.

Blogs on GFP. Showing 1-10 of 12 blog posts - Show all blog posts.

Autophagy and RAS signaling: Clinical implications
By Christina Towers, PhD The cellular recycling process known as autophagy is currently being targeted in over 60 clinical trials focused on treating different types of cancer1. To date, the only autophagy-targeted...  Read full blog post.

Neurovascular signaling for repair enhances brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.

Make each cell count: How to assess autophagy using flow cytometry
Kristy R. Howell, PhDThe cellular recycling process known as autophagy may be induced by a variety of conditions including reduced nutrient availability, serum starvation and pharmacological agents (e.g., Rapamyci...  Read full blog post.

How to visualize autophagy by microscopy
By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to...  Read full blog post.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

Best way to quantitatively measure Autophagic Flux
By Christina Towers, PhD Autophagy is a stress-induced cellular recycling process that plays an important physiological role in many diseases. It is induced by a variety of stimuli, both intracellular and extracel...  Read full blog post.

Animal Models to Study Autophagy
By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

GFP - Be Green!
Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

GFP (Green Fluorescent Protein) Bringing Light to Life
GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

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Recent Reviews

4.8
5
5
4
1
3
0
2
0
1
0

Anonymous
03/08/2020
Application: WB
Species: Mouse

Anonymous
10/21/2019
Application: IF
Species: Mouse

Anonymous
05/08/2018
Application: ICC
Species: cells