GFP Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Analysis using the Biotin conjugate of NB100-1770. Staining of transgenic mouse pancreas, expressing GFP in beta cells. Image courtesy of product ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Analysis of Biotin conjugate of NB100-1770. Tissue: Drosophila melanogaster late stage embryonic central nervous system. Fixation: 0.5% PFA. Antigen ...read more
Genetic Strategies: Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Analysis using the Biotin conjugate of NB100-1770. Staining of GFP in mouse aorta, liver, brain, and lung cells. Image ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Antibody. Tissue: E5.5 Hex-GFP transgenic mouse embryo. Primary antibody: Goat anti-GFP was used at 1:500 dilution. Secondary antibody: Fluorochrome ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - IF analysis of GFP in mouse brain. Image courtesy of product review by Tatyana Pivneva.
Western Blot: GFP Antibody [NB100-1770] - Analysis using the Alkaline Phosphatase conjugate of NB100-1770. Detection of Lane 1: GFP. Detection of Lane 2: none. Load: 50 ng per lane. Alkaline Phosphatase GFP secondary ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - HEK293 cells stable transfected with integrin alpha8-GFP, were immunostained for GFP (green) and counterstained with phalloidin (red) and DAPI (BLUE). ...read more
Immunohistochemistry: GFP Antibody [NB100-1770] - GFP-positive transplanted NPCs have morphological features of hippocampal pyramidal neurons at day 90 after grafting. Image from verified customer review.
Western Blot: GFP Antibody [NB100-1770] - Comparison of fluorescence absorption and emission spectra for DyLight (TM) 488 and Alexa Fluor 488 in PBS, pH7.2. The emission spectra for this DyLight (TM) conjugate match the ...read more
Western Blot: GFP Antibody [NB100-1770] - Analysis using the FITC conjugate of NB100-1770. Detection of Lane 1: GFP. Detection of Lane 2: None. Load: 50ug per lane. Primary antibody: None. Secondary antibody: ...read more
Western Blot: GFP Antibody [NB100-1770] - Analysis using Biotin conjugate of NB100-1770. Lane 1: GFP. Lane 2: None. Load: 50 ng per lane. Primary antibody: GFP antibody Biotin conjugated at 1:1,000 for 60 min at RT. ...read more
Western Blot: GFP Antibody [NB100-1770] - Analysis using Biotin conjugate of NB100-1770. Lane 1: HeLa cells. Lane 2: mock transfected HeLa cell lysate. Load: 35 ug per lane. Primary antibody: GFP antibody at 1 ug/ml for ...read more
Western Blot: GFP Antibody [NB100-1770] - Analysis using HRP conjugate of NB100-1770. Lane 1: 50ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti- Peroxidase Conjugate secondary antibody was used ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Analysis using the Biotin conjugate of NB100-1770. Staining of GFP-Hela cells against a Streptavidin conjugated 550 secondary. Hela-GFP are ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Analysis of DyLight 488 conjugate of NB100-1770. Tissue: human breast carcinoma. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary ...read more
ELISA: GFP Antibody [NB100-1770] - Analysis using the DyLight 488 conjugate of NB100-1770. Detection of Lane 1: His-Sumo-GFP. Lane: Molecular Weight. Detection of Lane 2: Beta-Galactosidase (negative control). Detection ...read more

Product Details

Summary
Reactivity JfSpecies Glossary
Applications WB, ELISA, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, IHC-FrFl
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

GFP Antibody Summary

Immunogen
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GFP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:400 - 1:2000
  • Electron Microscopy 1:10-1:500
  • ELISA 1:40000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Proximity Ligation Assay
  • Immunohistochemistry Free-Floating 1:10 - 1:500
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24314556) Although not tested this antibody may be useful in Immunoprecipitation. Use in Immunohistochemistry free floating reported in scientific literature (PMID 24753232) Use in Electron Microscopy reported in scientific literature (PMID 24753232). Use in Proximity Ligation Assay reported in scientific literature (PMID 24470503). Use in FLOW cytometry reported in scientific literature ( PMID 23682015)
Reviewed Applications
Read 4 Reviews rated 5
using
NB100-1770 in the following applications:

Publications
Read Publications using
NB100-1770 in the following applications:

Reactivity Notes

No reaction was observed against Human, or Rat serum proteins. Known Cross Reactivity: rGFP. YFP differs from GFP due to a mutation at Thr203Tyr; antibodies raised against full-length GFP should also detect YFP and other variants. Reactivity in transgenic mice with GFP. Reactivity in human cell lines transfected will a GFP construct. Mouse reactivity reported in multiple pieces of scientific literature.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GFP Antibody

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

Green fluorescence protein (GFP) is a 27 KDa protein derived from the jellyfish Aquorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB100-1770)(66)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Firefly, Invertebrate, Jellyfish, Sheep, Virus.

We have publications tested in 11 applications: ELISA, EM, FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-FrFl, IHC-P, PLA, WB.


Filter By Application
ELISA
(2)
EM
(2)
FLOW
(1)
Flow
(2)
ICC/IF
(13)
IHC
(11)
IHC-Fr
(10)
IHC-FrFl
(1)
IHC-P
(5)
PLA
(2)
WB
(2)
All Applications
Filter By Species
Human
(5)
Mouse
(14)
Rat
(1)
Firefly
(1)
Invertebrate
(1)
Jellyfish
(8)
Sheep
(1)
Virus
(1)
All Species
Showing Publications 1 - 10 of 66. Show All 66 Publications.
Publications using NB100-1770 Applications Species
Asai Y, Pan B, Nist-Lund C et al. Transgenic Tmc2 expression preserves inner ear hair cells and vestibular function in mice lacking Tmc1 Sci Rep Aug 14 2018 [PMID: 30108254] (IHC, Jellyfish) IHC Jellyfish
Sonzogni O, Haynes J, Seifried LA et al. Reporters to mark and eliminate basal or luminal epithelial cells in culture and in vivo PLoS Biol. PLoS Biol 2018 Jun 20 [PMID: 29924804] (ICC/IF, Jellyfish)

Details:
This citation used the Biotin version of this antibody.
ICC/IF Jellyfish
Roh HC, TSai LTY, Shao M et al. Warming Induces Significant Reprogramming of Beige, but Not Brown, Adipocyte Cellular Identity. Cell Metab 2018 Apr 5 [PMID: 29657031] (Jellyfish)

Details:
This citation used the Biotin version of this antibody.
Jellyfish
Huang B, Li X, Tu X et al. OTX1 regulates cell cycle progression of neural progenitors in the developing cerebral cortex. J. Biol. Chem. 2017 Dec 22 [PMID: 29273633] (Mouse) Mouse
O M Tsupykov, A O Poddubnaya, K G Smozhanyk, V M Kyryk O V Kuchouk et al. Integration of Grafted Neural Progenitor Cells in a Host Hippocampal Circuitry after Ischemic Injury. Neurophysiology, Volume 43, Number 4, Pages 324-326. 2011 (IHC, Mouse) IHC Mouse
Gao Y, Hu S, Li Q et al. Neonatal inflammation induces reorganization in dendritic morphology of retinal ganglion cells but not their retinogeniculate projection in mice. Neurosci. Lett. Apr 5 2018 [PMID: 29627341] (IHC, Jellyfish) IHC Jellyfish
Tata PR, Chow RD, Saladi SV et al. Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity. Dev. Cell. Mar 26 2018 [PMID: 29587142] (Jellyfish) Jellyfish
Wang J, Li Y, King R, Struebing FL. Optic nerve regeneration in the mouse is a complex trait modulated by genetic background. Mol. Vis. 2018 Feb 15 [PMID: 29463955] (ICC/IF, Jellyfish) ICC/IF Jellyfish
Relation T, Yi T, Guess AJ, La Perle K. Intratumoral Delivery of Interferon gamma-secreting MSCs Repolarizes Tumor-Associated Macrophages and Suppresses Neuroblastoma Proliferation In Vivo. Stem Cells. 2018 Feb 12 [PMID: 29430789] (ICC/IF, Jellyfish)

Details:
This citation used the Biotin form of this antbody
ICC/IF Jellyfish
Show All 66 Publications.

Reviews for GFP Antibody (NB100-1770) (4) 54

Average Rating: 5
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Mouse, cells.

Reviews using NB100-1770:
Filter by Applications
ICC
(1)
IF
(1)
IHC
(2)
All Applications
Filter by Species
Mouse
(3)
cells
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry GFP NB100-1770
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reviewed by:
Anonymous
ICC cells 05/08/2018
View

Summary

ApplicationImmunocytochemistry
Sample TestedItga8-GFP transfected HEK293 cells
Speciescells
LotNA
 
reviewed by:
Anonymous
IF Mouse 12/12/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24753232
SpeciesMouse

Blocking

Blocking DetailsSee PMID 24753232

Details

Detection NotesSee PMID 24753232

Comments

CommentsPublished in PMID: 24753232
Immunohistochemistry GFP NB100-1770
Enlarge
reviewed by:
Anonymous
IHC Mouse 06/19/2014
View

Summary

ApplicationImmunohistochemistry
SpeciesMouse
Immunohistochemistry GFP NB100-1770
Enlarge
reviewed by:
Anonymous
IHC Mouse 05/15/2012
View

Summary

ApplicationImmunohistochemistry
Sample Tested Mouse brain
SpeciesMouse
Lot25297

Blocking

Blocking Details2% BSA and 0.3% Triton X-100 for 2 hours at RT

Primary Anitbody

Dilution Ratiodilution 1:5000 for 24 hours at 4 C

Secondary Antibody

Secondary Descriptiondonkey anti-goat Alexa 488
Secondary Concentration1:1000

Details

Fixation Detailsfixation by 4% paraformaldehyde, permeabilization by 0.3% Triton X-100
Wash Descriptionwashing by PBS

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFP Antibody (NB100-1770). (Showing 1 - 1 of 1 FAQs).

  1. Does this antibody NB100-1770 detect eGFP?
    • NB100-1770 has not yet been tested against eGFP by our lab. Theoretically, you should be able to detect the eGFP with this antibody, as the two are very homologous; however, we cannot guarantee it. If you would like to test an antibody in an untested species and/or application and share your results with us, I can recommend our Innovator's Reward program. Our Innovator's Reward program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Reviews may also be submitted by emailing Innovators Reward.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for GFP Antibody (NB100-1770)

Discover related pathways, diseases and genes to GFP Antibody (NB100-1770). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on GFP.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

GFP - Be Green!
Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

GFP (Green Fluorescent Protein) Bringing Light to Life
GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

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Recent Reviews

5
5
4
4
0
3
0
2
0
1
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Anonymous
05/08/2018
Application: ICC
Species: cells

 
Anonymous
12/12/2014
Application: IF
Species: Mouse

Anonymous
06/19/2014
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol GFP