GFP Antibody (4B10B2) [PE]

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Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Flow
Clone
4B10B2
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

Order Details

GFP Antibody (4B10B2) [PE] Summary

Immunogen
This GFP antibody was developed against purified recombinant fragment of GFP expressed in E. coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Reacts with GFP.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Ammonium sulfate precipitation

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for GFP Antibody (4B10B2) [PE]

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T.,... Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S.,... Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NBP2-22111PE) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for GFP Antibody (NBP2-22111PE). (Showing 1 - of FAQ).

    Secondary Antibodies

     

    Isotype Controls

    Other Available Formats

    Alexa Fluor 350 NBP2-22111AF350
    Alexa Fluor 405 NBP2-22111AF405
    Alexa Fluor 488 NBP2-22111AF488
    Alexa Fluor 532 NBP2-22111AF532
    Alexa Fluor 594 NBP2-22111AF594
    Alexa Fluor 647 NBP2-22111AF647
    Alexa Fluor 700 NBP2-22111AF700
    Alexa Fluor 750 NBP2-22111AF750
    Allophycocyanin NBP2-22111APC
    Allophycocyanin/Cy7 NBP2-22111APCCY7
    Biotin NBP2-22111B
    DyLight 350 NBP2-22111UV
    DyLight 405 NBP2-22111V
    DyLight 488 NBP2-22111G
    DyLight 550 NBP2-22111R
    DyLight 594 NBP2-22111DL594
    DyLight 650 NBP2-22111C
    DyLight 680 NBP2-22111FR
    DyLight 755 NBP2-22111IR
    FITC NBP2-22111F
    HRP NBP2-22111H
    Janelia Fluor 549 NBP2-22111JF549
    Janelia Fluor 646 NBP2-22111JF646
    PE NBP2-22111PE
    PE/Atto594 NBP2-22111PEATT594
    PE/Cy5.5 NBP2-22111PECY55
    PE/Cy7 NBP2-22111PECY7
    PerCP NBP2-22111PCP

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    Array NBP2-22111PE

    Research Areas for GFP Antibody (NBP2-22111PE)

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    Neurovascular signaling for repair enhances brain metastasis
    By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.


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    Toll-like receptors in the intestinal epithelial cells
    By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.


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    The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
    GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

    GFP - Be Green!
    Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

    GFP (Green Fluorescent Protein) Bringing Light to Life
    GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

    BDNF Antibodies Aid Research on Alzheimer's Therapies
    Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

    Showing 1-10 of 11 blog posts - Show all blog posts.
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