GFP Antibody [DyLight 488]


Western Blot: GFP Antibody [DyLight 488] [NBP1-69969] - Lane 1: His-Sumo-GFP. Lane: Molecular Weight. Lane 2: Beta-Galactosidase (negative control). Lane 3: recombinant GFP control protein. Load: 35 ug per lane. Primary more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [DyLight 488] [NBP1-69969] - Tissue: human breast carcinoma. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Anti-Histone and Anti-Tubulin more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [DyLight 488] [NBP1-69969] - Comparison of fluorescence absorbtion and emission spectra for DyLight (TM) 488 and Alexa Fluor 488 in PBS, pH7.2. The emission spectra more

Product Details

Reactivity InSpecies Glossary
Applications WB, ICC/IF
LYOPH mg/ml

Order Details

GFP Antibody [DyLight 488] Summary

Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish (Aequorea victoria).
GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
DyLight 488
DyLight 488 (A=493, E=518)
20 mM Potassium Phosphate (pH 7.2), 150 mM NaCl, and 10 mg/ml BSA (immunoglobulin and protease free)
0.01% Sodium Azide
LYOPH mg/ml
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.


  • Western Blot 1:10000 - 1:25000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
The emission spectra for this DyLight (TM) conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Reactivity Notes

No reaction was observed against Human, or Rat serum proteins. Known Cross Reactivity: rGFP. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants. Reactivity in transgenic mice with GFP. Reactivity in human cell lines transfected will a GFP construct. Mouse reactivity reported in multiple pieces of scientific literature.


Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for GFP Antibody [DyLight 488]

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein


The emission spectra for this DyLight conjugate match the principle output wavelengths of most common fluorescence instrumentation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFP Antibody (NBP1-69969). (Showing 1 - of FAQ).

Secondary Antibodies


Isotype Controls

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Bioinformatics Tool for GFP Antibody (NBP1-69969)

Discover related pathways, diseases and genes to GFP Antibody (NBP1-69969). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on GFP.

GFP - Be Green!
Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

GFP (Green Fluorescent Protein) Bringing Light to Life
GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol GFP

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