GFP Antibody (9F9.F9)

Images

 
Immunocytochemistry/ Immunofluorescence: GFP Antibody (9F9.F9) [NB600-597] - GFP-transducted 293T cells. Image from verified customer review.
Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Lane 1: Molecular Weight Markers. Lane 2: GFP. Load: 10ng per lane. Primary antibody: Ms Anti-GFP antibody at 1:1000 for overnight at 4C. Secondary antibody: Mouse HRP ...read more
Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Lane 1: 64pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 2: 32pg of recombinant GFP protein were spiked into a HeLa cell-derived ...read more
Immunohistochemistry: GFP Antibody (9F9.F9) [NB600-597] - Used 1:5000 As referenced in: Lu S-M, Tremblay M-E`, King IL, Qi J, Reynolds HM, et al. (2011) HIV-1 Tat-Induced Microgliosis and Synaptic Damage via ...read more
Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Expression of GFP in CHO-K1 cells trasfected with GFP. Image from verified customer review.

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, DB, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, In vitro, IP, KD
Clone
9F9.F9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Catalog# & Conjugate Size Price

GFP Antibody (9F9.F9) Summary

Immunogen
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
Specificity
GFP antibody is observed to react against wild type and recombinant forms of GFP. No reaction is seen against RFP.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:3000 - 1:30000
  • Dot Blot 1:100 - 1:2000
  • ELISA 1:10000-1:30000
  • Flow Cytometry 1:1:10 - 1:1000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
  • Knockdown Validated
Application Notes
Use in In vitro assay reported in scientific literature (PMID 23125142) Use in dot blot reported in scientific literature (PMID 24497578). Use in immunoprecipitation reported in scientific literature (PMID 28198010). Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Knockdown validation (Wolf et al). Use in immunoblotting reported in scientific literature (PMID:31727786).
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB600-597 in the following applications:

Publications
Read Publications using
NB600-597 in the following applications:

  • DB
    1 publication
  • 3 publications
  • IB
    1 publication
  • 7 publications
  • IHC
    3 publications
  • 1 publication
  • IP
    3 publications
  • 1 publication
  • KD
    1 publication
  • WB
    7 publications

Reactivity Notes

Recognizes fusion proteins in Drosophila, reactivity reported in scientific literature (PMID: 26586808). Human reactivity reported in scientific literature (PMID: 28198010).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for GFP Antibody (9F9.F9)

  • clone 9F9.F9
  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP monoclonal
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T.,... Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S.,... Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB600-597)(45)

We have publications tested in 2 confirmed species: Human, Plants.

We have publications tested in 10 applications: DB, ELISA, IB, ICC/IF, IHC, IHC-P, IP, In vitro, KD, WB.


Filter By Application
DB
(1)
ELISA
(3)
IB
(1)
ICC/IF
(7)
IHC
(3)
IHC-P
(1)
IP
(3)
In vitro
(1)
KD
(1)
WB
(7)
All Applications
Filter By Species
Human
(1)
Plants
(1)
All Species
Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using NB600-597 Applications Species
Wolf EJ, Miles A, Lee ES et al. MKRN2 Physically Interacts with GLE1 to Regulate mRNA Export and Zebrafish Retinal Development Cell Rep May 26 2020 [PMID: 32460013] (WB) WB
Higuchi C, Yamamoto M, Shin SW et al. Perturbation of maternal PIASy abundance disrupts zygotic genome activation and embryonic development via SUMOylation pathway Biol Open Oct 24 2019 [PMID: 31640975] (WB) WB
Redditt TJ, Chung EH, Zand Karimi H et al. AvrRpm1 Functions as an ADP-Ribosyl Transferase to Modify NOI-domain Containing Proteins, Including Arabidopsis and Soybean RPM1-interacting Protein 4 Plant Cell Sep 25 2019 [PMID: 31548257] (WB) WB
Zusso M, Lunardi V, Franceschini D et al. Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway J Neuroinflammation Jul 20 2019 [PMID: 31319868] (IP, WB) IP, WB
Carter M, Helm M, Chapman A et al. Convergent evolution of effector protease recognition by Arabidopsis and barley. bioRxiv Jul 23 2018 [PMID: 30480480]
Methot L, Soubannier V, Hermann R et al. Nuclear factor-kappaB regulates multiple steps of gliogenesis in the developing murine cerebral cortex Glia Oct 20 2018 [PMID: 30338559] (ICC/IF) ICC/IF
Dumon C, Diabira D, Chudotvorova I et al. The adipocyte hormone leptin sets the emergence of hippocampal inhibition in mice Elife Aug 15 2018 [PMID: 30106375] (ICC/IF) ICC/IF
Ping S, Qiu X, Gonzalez-Toledo ME et al. Stem Cell Factor in Combination with Granulocyte Colony-Stimulating Factor reduces Cerebral Capillary Thrombosis in a Mouse Model of CADASIL Cell Transplant Jun 7 2018 [PMID: 29871518] (IHC) IHC
Fulop G, Brameshuber M, Arnold AM et al. Determination of the Membrane Environment of CD59 in Living Cells Biomolecules May 17 2018 [PMID: 29772810] Biomolecules. 2018 May 17 [PMID: 29772810]

Details:
Citation using the Biotin form of this antibody.
Bocchi R, Egervari K, Carol-Perdiguer L et al. Perturbed Wnt signaling leads to neuronal migration delay, altered interhemispheric connections and impaired social behavior Nat Commun Oct 29 2017 [PMID: 29079819]
Show All 45 Publications.

Reviews for GFP Antibody (NB600-597) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Other.

Reviews using NB600-597:
Filter by Applications
WB
(2)
ICC
(1)
All Applications
Filter by Species
Human
(2)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot GFP NB600-597
Enlarge
4
reviewed by:
Feng Li
WB Human 11/18/2019
View

Summary

ApplicationWestern Blot
Sample Tested293 cell extract
SpeciesHuman
Lot37157
Immunocytochemistry GFP NB600-597
Enlarge
5
reviewed by:
Anonymous
ICC Human 05/10/2018
View

Summary

ApplicationImmunocytochemistry
Sample TestedHEK-293T cells transduced to have GFP
SpeciesHuman
Lotlot# 200ug/100ul

Comments

CommentsThe 293T cells have been transduced to have GFP which was detected by an antibody after permeabilization.
The green staining shows the anti-GFP antibody.
Western Blot GFP NB600-597
Enlarge
5
reviewed by:
Anonymous
WB Other 01/26/2015
View

Summary

ApplicationWestern Blot
Sample TestedCHOK1 cells transfected with GFP
SpeciesOther
Lot28856

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratio1:3000 overnight at 4C in blocking buffer

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, HRP
Secondary Manufacturer Cat#Cell Signaling, #7076
Secondary Concentration1:5000

Details

Detection Notes15-second exposure shows strong band at correct MW.

Comments

CommentsThis GFP antibody works very well. Very clear background. Five star!

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFP Antibody (NB600-597). (Showing 1 - of FAQs).

    Secondary Antibodies

     

    Isotype Controls

    Other Available Formats

    Biotin NB110-40670

    Additional GFP Products

    Research Areas for GFP Antibody (NB600-597)

    Find related products by research area.

    Blogs on GFP. Showing 1-10 of 12 blog posts - Show all blog posts.

    Autophagy and RAS signaling: Clinical implications
    By Christina Towers, PhD The cellular recycling process known as autophagy is currently being targeted in over 60 clinical trials focused on treating different types of cancer1. To date, the only autophagy-targeted...  Read full blog post.

    Neurovascular signaling for repair enhances brain metastasis
    By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.

    Make each cell count: How to assess autophagy using flow cytometry
    Kristy R. Howell, PhDThe cellular recycling process known as autophagy may be induced by a variety of conditions including reduced nutrient availability, serum starvation and pharmacological agents (e.g., Rapamyci...  Read full blog post.

    How to visualize autophagy by microscopy
    By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to...  Read full blog post.

    Toll-like receptors in the intestinal epithelial cells
    By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

    Best way to quantitatively measure Autophagic Flux
    By Christina Towers, PhD Autophagy is a stress-induced cellular recycling process that plays an important physiological role in many diseases. It is induced by a variety of stimuli, both intracellular and extracel...  Read full blog post.

    Animal Models to Study Autophagy
    By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.

    The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
    GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

    GFP - Be Green!
    Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

    GFP (Green Fluorescent Protein) Bringing Light to Life
    GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

    Showing 1-10 of 12 blog posts - Show all blog posts.

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    Recent Reviews

    4.7
    5
    2
    4
    1
    3
    0
    2
    0
    1
    0

    Feng Li
    11/18/2019
    Application: WB
    Species: Human

    Anonymous
    05/10/2018
    Application: ICC
    Species: Human

    Anonymous
    01/26/2015
    Application: WB
    Species: Other