GFP Antibody (9F9.F9)

Images

 
Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Lane 1: Molecular Weight Markers. Lane 2: GFP. Load: 10ng per lane. Primary antibody: Ms Anti-GFP antibody at 1:1000 for overnight at 4C. Secondary antibody: Mouse HRP ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody (9F9.F9) [NB600-597] - GFP-transducted 293T cells. Image from verified customer review.
Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Expression of GFP in CHO-K1 cells trasfected with GFP. Image from verified customer review.
Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Analysis using the Biotin conjugate of NB600-597. Detection of Green Fluorescent Protein. 50ng of GFP (Lane 1) was run on a 4-20% gel and transferred to 0.45 um ...read more
Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Lane 1: 64pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 2: 32pg of recombinant GFP protein were spiked into a HeLa cell-derived ...read more
Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Recombinantly expressed GFP in Hela. Lane 1: HeLa lysate 50 ng. Lane 2: HeLa lysate 100 ng. Lane 3: HeLa lysate 500 ng. Primary antibody: GFP antibody at 1.0 mg/ml for ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody (9F9.F9) [NB600-597] - Analysis of Biotin conjugate of NB600-597. Used 1:5000 As referenced in: Lu S-M, Tremblay M-E`, King IL, Qi J, Reynolds HM, et al. (2011) ...read more
SDS-Page: GFP Antibody (9F9.F9) [NB600-597] - SDS PAGE of Mouse anti-GFP antibody. Lane 1: GFP non-reduced. Lane 2: Molecular Weight Markers. Lane 3: GFP reduced. Load: 10 ug per lane. Other band(s): none.

Product Details

Summary
Reactivity JfSpecies Glossary
Applications WB, DB, ELISA, Flow, ICC/IF, IHC, IHC-P, In vitro, IP, PAGE
Clone
9F9.F9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price

GFP Antibody (9F9.F9) Summary

Immunogen
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
Specificity
Reactivity is observed against wild type and recombinant forms of GFP. No reaction is seen against RFP.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GFP
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:3000 - 1:30000
  • Dot Blot 1:100-1:2000
  • ELISA 1:80000 - 1:500000
  • Flow Cytometry 1:1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
  • SDS-Page 1:100-1:2000
Application Notes
Use in In vitro assay reported in scientific literature (PMID 23125142) Use in dot blot reported in scientific literature (PMID 24497578). Use in immunoprecipitation reported in scientific literature (PMID 28198010).
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
NB600-597 in the following applications:

Publications
Read Publications using
NB600-597 in the following applications:

Reactivity Notes

Recognizes fusion proteins in Drosophila, reactivity reported in scientific literature (PMID: 26586808). Human reactivity reported in scientific literature (PMID: 28198010).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Alternate Names for GFP Antibody (9F9.F9)

  • clone 9F9.F9
  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP monoclonal
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

Green fluorescence protein (GFP) is a 27 KDa protein derived from the jellyfish Aquorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB600-597)(31)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Drosophila, Firefly, Jellyfish.

We have publications tested in 6 applications: DB, ELISA, ICC/IF, IHC-P, IP, In vitro.


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DB
(1)
ELISA
(3)
ICC/IF
(6)
IHC-P
(1)
IP
(1)
In vitro
(1)
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Filter By Species
Human
(3)
Mouse
(4)
Rat
(1)
Drosophila
(1)
Firefly
(1)
Jellyfish
(2)
All Species
Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using NB600-597 Applications Species
Dumon C, Diabira D, Chudotvorova I et al. The adipocyte hormone leptin sets the emergence of hippocampal inhibition in mice Elife Aug 14 2018 [PMID: 30106375] (ICC/IF, Jellyfish) ICC/IF Jellyfish
Bocchi R, Egervari K, Carol-Perdiguer L et al. Perturbed Wnt signaling leads to neuronal migration delay, altered interhemispheric connections and impaired social behavior. Nat Commun. 2017 Oct 27 [PMID: 29079819] (Jellyfish) Jellyfish
Della Pia EA, Martinez KL. Single domain antibodies as a powerful tool for high quality surface plasmon resonance studies PLoS ONE 2015 Mar 31 [PMID: 25822527]

Details:
This citation used the Biotin version of this antibody.
Sohn YI, Lee NJ, Chung A et al. Antihypertensive drug Valsartan promotes dendritic spine density by altering AMPA receptor trafficking. Biochem Biophys Res Commun. 2013 Oct 4 [PMID: 24012668] (ICC/IF) ICC/IF
Della Pia EA, Martinez KL. Single domain antibodies as a powerful tool for high quality surface plasmon resonance studies PLoS ONE

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NB110-40670).
Zusso M, Mercanti G, Belluti F et al. Phenolic 1,3-diketones attenuate lipopolysaccharide-induced inflammatory response by an alternative magnesium-mediated mechanism. Br. J. Pharmacol. 2017 Feb 15 [PMID: 28198010] (IP, Human) IP Human
Song JM, Sung YM, Nam JH et al. A Mercaptoacetamide-Based Class II Histone Deacetylase Inhibitor Increases Dendritic Spine Density via RasGRF1/ERK Pathway J. Alzheimers Dis. Feb 3 2016 [PMID: 26890742]
Boltengagen M, Huang A, Boltengagen A et al. A novel role for the histone acetyltransferase Hat1 in the CENP-A/CID assembly pathway in Drosophila melanogaster. Nucleic Acids Res. 2015 Nov 19 [PMID: 26586808] (ICC/IF, Drosophila) ICC/IF Drosophila
Kim M, Jeong Y, Chang YC. Extracellular matrix protein reelin regulate dendritic spine density through CaMKIIbeta. Neurosci Lett 2015 Jul 10 [PMID: 26003447]
Bin NR, Jung CH, Kim B et al. Chaperoning of closed syntaxin-3 through Lys46 and Glu59 in domain-1 of Munc18 is indispensable for mast cell exocytosis. J. Cell. Sci. 2015 Mar 20 [PMID: 25795302] (ICC/IF, Human) ICC/IF Human
Show All 31 Publications.

Reviews for GFP Antibody (NB600-597) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.

Reviews using NB600-597:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry GFP NB600-597
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reviewed by:
Anonymous
ICC Human 05/10/2018
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Summary

ApplicationImmunocytochemistry
Sample TestedHEK-293T cells transduced to have GFP
SpeciesHuman
Lotlot# 200ug/100ul

Comments

CommentsThe 293T cells have been transduced to have GFP which was detected by an antibody after permeabilization.
The green staining shows the anti-GFP antibody.
Western Blot GFP NB600-597
Enlarge
reviewed by:
Anonymous
WB Other 01/26/2015
View

Summary

ApplicationWestern Blot
Sample TestedCHOK1 cells transfected with GFP
SpeciesOther
Lot28856

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratio1:3000 overnight at 4C in blocking buffer

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, HRP
Secondary Manufacturer Cat#Cell Signaling, #7076
Secondary Concentration1:5000

Details

Detection Notes15-second exposure shows strong band at correct MW.

Comments

CommentsThis GFP antibody works very well. Very clear background. Five star!

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFP Antibody (NB600-597). (Showing 1 - of FAQs).

    Secondary Antibodies

     

    Isotype Controls

    Other Available Formats

    Biotin Labeled NB110-40670

    Additional Array Products

    Bioinformatics Tool for GFP Antibody (NB600-597)

    Discover related pathways, diseases and genes to GFP Antibody (NB600-597). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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    Research Areas for GFP Antibody (NB600-597)

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    Blogs on GFP.

    The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
    GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

    GFP - Be Green!
    Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

    GFP (Green Fluorescent Protein) Bringing Light to Life
    GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

    BDNF Antibodies Aid Research on Alzheimer's Therapies
    Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

    Epitope Tags and the V5 Tag Antibody
    We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

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    Recent Reviews

    5
    5
    2
    4
    0
    3
    0
    2
    0
    1
    0

    Anonymous
    05/10/2018
    Application: ICC
    Species: Human

    Anonymous
    01/26/2015
    Application: WB
    Species: Other

    Bioinformatics

    Gene Symbol GFP