GFP Antibody [Alkaline Phosphatase]


Western Blot: GFP Antibody [Alkaline Phosphatase] [NB600-1502] - Lane 1: GFP. Lane 2: none. Load: 50 ng per lane. Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Predicted/Observed size: 28 kDa more

Product Details

Reactivity InSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

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GFP Antibody [Alkaline Phosphatase] Summary

Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish (Aequorea victoria).
GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C in the dark.
Alkaline Phosphatase
Alkaline Phosphatase
0.05 M Tris Chloride (pH 8.0), 0.15 M NaCl, 0.001 M Magnesium Chloride, 0.0001M Zinc Chloride, and 50% (v/v) Glycerol
0.1% Sodium Azide
Immunogen affinity purified


  • Western Blot 1:500-1:2500
  • ELISA 1:2000-1:8000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Anti-GFP is designed - detect GFP and its variants. This antibody can be used to detect GFP by ELISA for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP.
Read Publication using NB600-1502.

Reactivity Notes

No reaction was observed against Human, or Rat serum proteins. Known Cross Reactivity: rGFP. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants. Reactivity in transgenic mice with GFP. Reactivity in human cell lines transfected will a GFP construct. Mouse reactivity reported in multiple pieces of scientific literature.

Alternate Names for GFP Antibody [Alkaline Phosphatase]

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein


Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB600-1502)(1)

We have publications tested in 1 application: ELISA.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GFP Antibody (NB600-1502). (Showing 1 - 1 of 1 FAQ).

  1. Is the western blot shown here with 50 ng purified GFP protein or 50ng of cell lysate containing GFP?
    • Our western blot image depicts 50ng of purified GFP protein.

Secondary Antibodies


Isotype Controls

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Bioinformatics Tool for GFP Antibody (NB600-1502)

Discover related pathways, diseases and genes to GFP Antibody (NB600-1502). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for GFP Antibody (NB600-1502)

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Blogs on GFP.

GFP - Be Green!
Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

GFP (Green Fluorescent Protein) Bringing Light to Life
GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol GFP

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