GFP Antibody

Images

 
Western Blot: GFP Antibody [NB600-303] - Analysis using the FITC conjugate of NB600-303. Detection of Lane 1: GFP. Load: 50 ug per lane. Primary antibody: none. Secondary antibody: Fluorescein conjugated Anti-GFP at ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-303] - Analysis of FITC conjugate of NB600-303. Staining of mouse spleen cells Tissue: spleen cells infected with MHV68-H2bYFP. As seen in: Collins CM, Speck ...read more
Western Blot: GFP Antibody [NB600-303] - WB analysis of GFP in various lysates. Image courtesy of product review by Emilie Leclerc.
Immunoprecipitation: GFP Antibody [NB600-303] - IP analysis of GFP in HeLa cell lysate. Image courtesy of anonymous customer review.
Western Blot: GFP Antibody [NB600-303] - Analysis of GFP HRP expression in HeLa/GFP cell lysate.
Western Blot: GFP Antibody [NB600-303] - Analysis using Biotin conjugate of NB600-303. Lane 1: 50ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti- Biotin Conjugated secondary antibody was used ...read more
Immunohistochemistry: GFP Antibody [NB600-303] - Eight-week-old Lgr5-GFP mice were injected with CCL4 and rHGF or/and rRspo1 proteins (0.1 ug/mice/time), as indicated in a, Lgr5 expression was measured using an ...read more
Western Blot: GFP Antibody [NB600-303] - Lane 1: 293FT cells transfected with CDK4 dominant negative. Lane 2: 293FT cells poitive control. Load: 25 ug per lane. Primary antibody: GFP antibody at 1:400 for overnight at ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-303]

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
6.05 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

GFP Antibody Summary

Immunogen
This GFP antibody was developed against a fusion protein corresponding to the full length GFP (246aa) derived from the jellyfish Aequorea victoria.
Specificity
GFP antibody recognizes GFP from Aequorea victoria
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:3000
  • Simple Western
  • Chromatin Immunoprecipitation
  • Electron Microscopy
  • ELISA 1:10000 - 1:40000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
Immunoprecipitation reported successful by customer review. Immunohistochemistry-Paraffin (PMID: 21311774) Immunocytochemistry/Immunofluorescence was reported in scientific literature.Use in Flow Cytometry reported in scientific literature (PMID: 23903657). Use in Electron Microscopy reported in scientific literature (PMID 25538186). Use in Immunohistochemistry-Frozen reported in multiple pieces of scientific literature. Use in chromatin immunoprecipitation reported in scientific literature (PMID 28174757).
Simple Western reported by an internal validation. Separated by Size-Jess/Wes, Sally Sue/Peggy Sue; matrix was 12-230 kDa.
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4
using
NB600-303 in the following applications:

Publications
Read Publications using
NB600-303 in the following applications:

  • 2 publications
  • EM
    1 publication
  • 1 publication
  • 5 publications
  • IHC
    3 publications
  • 1 publication
  • 7 publications
  • IP
    2 publications
  • WB
    12 publications

Reactivity Notes

GFP recombinant expression in mouse reported in scientific literature (PMID: 27106802). Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP. Human reactivity reported in scientific literature (PMID: 28174757). Mouse reactivity reported in scientific literature (PMID: 29079780).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
6.05 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GFP Antibody

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T.,... Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S.,... Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB600-303)(50)

We have publications tested in 1 confirmed species: Jellyfish.

We have publications tested in 9 applications: ChIP, EM, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ChIP
(2)
EM
(1)
FLOW
(1)
ICC/IF
(5)
IHC
(3)
IHC-Fr
(1)
IHC-P
(7)
IP
(2)
WB
(12)
All Applications
Filter By Species
Jellyfish
(1)
All Species
Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using NB600-303 Applications Species
Kanayama K, Takada H, Saito N et al. Hair regeneration potential of human dermal sheath cells cultured under physiological oxygen Tissue Eng Part A May 14 2020 [PMID: 32408803]
Bassermann AG. Dissertation_Baumann_vorlaufig Thesis. 2016 (Jellyfish) Jellyfish
Fu DJ, Wang L, Chouairi FK et al. Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5-CD44+ cells Nat Commun Jan 3 2020 [PMID: 31901081] (IHC-P) IHC-P
Kotagama K, Schorr AL, Steber HS et al. ALG-1 Influences Accurate mRNA Splicing Patterns in the Caenorhabditis elegans Intestine and Body Muscle Tissues by Modulating Splicing Factor Activities Genetics May 11 2019 [PMID: 31073019] (WB, IP) WB, IP
Barenz F, Kschonsak YT, Meyer A et al. Ccdc61 controls centrosomal localization of Cep170 and is required for spindle assembly and symmetry Mol Biol Cell Oct 26 2018 [PMID: 30354798] (WB) WB
Lobs AK, Schwartz C, Thorwall S et al. Highly Multiplexed CRISPRi Repression of Respiratory Functions Enhances Mitochondrial Localized Ethyl Acetate Biosynthesis in Kluyveromyces marxianus ACS Synth Biol Oct 26 2018 [PMID: 30354074] (WB) WB
Lagunas A M, Wu J et al. Telomere DNA damage signaling regulates cancer stem cell evolution, epithelial mesenchymal transition, and metastasis. Oncotarget Jun 10 2017 [PMID: 29113290] (IHC) IHC
Lin Y, Fang ZP, Liu HJ et al. HGF/R-spondin1 rescues liver dysfunction through the induction of Lgr5(+) liver stem cells Nat Commun Oct 29 2017 [PMID: 29079780] (ICC/IF) ICC/IF
Seneviratne APB, Turan Z, Hermant A et al. Modulation of estrogen related receptor alpha activity by the kinesin KIF17 Oncotarget Sep 9 2017 [PMID: 28881568] (WB) WB
Almohazey D, Lo YH, Vossler CV et al. The ErbB3 receptor tyrosine kinase negatively regulates Paneth cells by PI3K-dependent suppression of Atoh1. Cell Death Differ. May 1 2017 [PMID: 28304405] (ChIP) ChIP
Show All 50 Publications.

Reviews for GFP Antibody (NB600-303) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Other.

Reviews using NB600-303:
Filter by Applications
WB
(2)
IP
(1)
All Applications
Filter by Species
Human
(2)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot GFP NB600-303
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5
reviewed by:
Anonymous
WB Human 11/18/2011
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
CommentsThe antibody worked very well in western blot experiments.
Pub Med ID21777220

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:2500

Details

Detection NotesDetection Method: Lumilight (Roche)

Comments

CommentsThe antibody worked very well in western blot experiments.
Immunoprecipitation GFP NB600-303
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4
reviewed by:
Marisa Carbonaro
IP Human 09/27/2010
View

Summary

ApplicationImmunoprecipitation
Sample TestedHeLa cells
SpeciesHuman
CommentsWorks very well for IP of GFP-tagged proteins. Got very clean results. Lysate Amount: 1mg

Comments

CommentsWorks very well for IP of GFP-tagged proteins. Got very clean results. Lysate Amount: 1mg
  3
reviewed by:
Anonymous
WB Other 03/02/2009
View

Summary

ApplicationWestern Blot
Sample Testedrecombinant protein, Sample Amount: 0.5 - 1 ug
SpeciesOther
Lot21194
CommentsAll my samples have been treated with DNase - it appears in both the sample and the negative control.

Blocking

Blocking DetailsBlocking Buffer: PBS, 5% milk, Blocking Time: 20 minutes, Blocking Temp: 20 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/2500, Incubation Dilution Buffer: PBS+3% dry milk, Incubation Time: 90min, Incubation Temp: 20C

Secondary Antibody

Secondary DescriptionSecondary Ab: pAb goat anti-rabbit IgG, conj. AP, Secondary Ab Dilution Ratio: 1/10000
Secondary Manufacturer Cat#NB730-AP

Details

Detection NotesDetection Method:AP reaction, Exposure Time:10-30min, Neg Ctrl: sample w/o expressed protein, Non-Specific Band:~35kDa

Comments

CommentsAll my samples have been treated with DNase - it appears in both the sample and the negative control.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for GFP Antibody (NB600-303). (Showing 1 - 3 of 3 FAQs).

  1. I would like to know if ab290 from Abcam is identical to NB600-303?
    • Both antibodies (i.e. # ab290 from Abcam and # NB600-303 from Novus) use the same immunogen to develop this antibody. Both companies used highly purified recombinant full length protein made in E. coli as immunogen and this antibody is directed against the entire GFP molecule. We stand behind our products 100% and would be there to help in case you comes up with any concerns. Moreover our antibody is also well tested and has been cited in recent research papers in journals of high repute (Dis Model Mech. 2011 May;4(3):359-67; PLoS ONE. 2011; J Cell Sci. 2010 May 15;123(Pt 10):1732-41; Acta Anat (Basel). 1991;141(1):82-4; FASEB J. 2009 Nov 25. and many more...).
  2. Can you tell me the concentration for the latest lot of product NB600-303.
    • The current lot (31271) of NB600-303 is provided at a concentration of 6.05mg/ml.
  3. Does this antibody also detect ECFP?
    • NB600-303 has been validated for detection of S65T-GFP, RS-GFP, YFP and EGFP. Based on an alignment to a ECFP sequence that I found on NCBI (ncbi site), the GFP protein this product was raised against does show a good amount of homology. Therefore, there would be a good chance for cross reaction of this product against ECFP.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin NB100-1365
FITC NB100-1183
FITC NB100-1183

Additional GFP Products

Research Areas for GFP Antibody (NB600-303)

Find related products by research area.

Blogs on GFP. Showing 1-10 of 12 blog posts - Show all blog posts.

Autophagy and RAS signaling: Clinical implications
By Christina Towers, PhD The cellular recycling process known as autophagy is currently being targeted in over 60 clinical trials focused on treating different types of cancer1. To date, the only autophagy-targeted...  Read full blog post.

Neurovascular signaling for repair enhances brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.

Make each cell count: How to assess autophagy using flow cytometry
Kristy R. Howell, PhDThe cellular recycling process known as autophagy may be induced by a variety of conditions including reduced nutrient availability, serum starvation and pharmacological agents (e.g., Rapamyci...  Read full blog post.

How to visualize autophagy by microscopy
By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to...  Read full blog post.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

Best way to quantitatively measure Autophagic Flux
By Christina Towers, PhD Autophagy is a stress-induced cellular recycling process that plays an important physiological role in many diseases. It is induced by a variety of stimuli, both intracellular and extracel...  Read full blog post.

Animal Models to Study Autophagy
By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

GFP - Be Green!
Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

GFP (Green Fluorescent Protein) Bringing Light to Life
GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

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Recent Reviews

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5
1
4
1
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1
2
0
1
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Anonymous
11/18/2011
Application: WB
Species: Human

Marisa Carbonaro
09/27/2010
Application: IP
Species: Human

 
Anonymous
03/02/2009
Application: WB
Species: Other