GFP Antibody

Images

 
Flow Cytometry: GFP Antibody [NB600-303] - Analysis of FITC conjugate of NB600-303. Staining of mouse spleen cells Tissue: spleen cells infected with MHV68-H2bYFP. As seen in: Collins CM, Speck SH (2012) Tracking Murine ...read more
Western Blot: GFP Antibody [NB600-303] - WB analysis of GFP in various lysates. Image courtesy of product review by Emilie Leclerc.
Western Blot: GFP Antibody [NB600-303] - Analysis of GFP HRP expression in HeLa/GFP cell lysate.
Western Blot: GFP Antibody [NB600-303] - Lane 1: Wild type GFP (0.1 ug ) was used to spike HeLa whole cell lysate. Lane 2: none. Load: 30 ug per lane. Primary antibody: GFP antibody at 1:1000 for overnight at 4C. ...read more
Western Blot: GFP Antibody [NB600-303] - Analysis using Biotin conjugate of NB600-303. Lane 1: 50ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti- Biotin Conjugated secondary antibody was used ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-303]
Western Blot: GFP Antibody [NB600-303] - Lane 1: 293FT cells transfected with CDK4 dominant negative. Lane 2: 293FT cells poitive control. Load: 25 ug per lane. Primary antibody: GFP antibody at 1:400 for overnight at ...read more
Western Blot: GFP Antibody [NB600-303] - 50ng of GFP HRP conjugate.
Western Blot: GFP Antibody [NB600-303] - Analysis using the FITC conjugate of NB600-303. Detection of Lane 1: GFP. Load: 50 ug per lane. Primary antibody: none. Secondary antibody: Fluorescein conjugated Anti-GFP at ...read more
Immunoprecipitation: GFP Antibody [NB600-303] - IP analysis of GFP in HeLa cell lysate. Image courtesy of anonymous customer review.

Product Details

Summary
Reactivity Hu, JfSpecies Glossary
Applications WB, ChIP, ELISA, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
6.0 mg/ml
     

Genetic Strategies

   

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

GFP Antibody Summary

Immunogen
Highly purified recombinant full length protein made in E. coli. The antibody is directed against the entire GFP molecule.
Specificity
GFP from Aequorea victoria
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GFP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:3000
  • Chromatin Immunoprecipitation
  • Electron Microscopy
  • ELISA 1:10000 - 1:40000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
Immunoprecipitation reported successful by customer review. Immunohistochemistry-Paraffin (PMID: 21311774) Immunocytochemistry/Immunofluorescence was reported in scientific literature.Use in Flow Cytometry reported in scientific literature (PMID: 23903657). Use in Electron Microscopy reported in scientific literature (PMID 25538186). Use in Immunohistochemistry-Frozen reported in multiple pieces of scientific literature. Use in chromatin immunoprecipitation reported in scientific literature (PMID 28174757).
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4
using
NB600-303 in the following applications:

Publications
Read Publications using
NB600-303 in the following applications:

Reactivity Notes

GFP recombinant expression in mouse reported in scientific literature (PMID: 27106802). Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP. Human reactivity reported in scientific literature (PMID: 28174757).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
Preservative
0.01% Sodium Azide
Concentration
6.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GFP Antibody

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

A highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin. In immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells. It is routinely used in immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP-immunohistochemistry (1:200) on whole-mount mouse embryos.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB600-303)(42)

We have publications tested in 7 confirmed species: Human, Mouse, Feline, Firefly, Fish, Plant, Primate.

We have publications tested in 8 applications: ChIP, EM, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ChIP
(1)
EM
(1)
FLOW
(1)
ICC/IF
(4)
IHC
(2)
IHC-Fr
(2)
IHC-P
(6)
WB
(7)
All Applications
Filter By Species
Human
(8)
Mouse
(4)
Feline
(1)
Firefly
(1)
Fish
(1)
Plant
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 42. Show All 42 Publications.
Publications using NB600-303 Applications Species
Lin Y, Fang ZP, Liu HJ et al. HGF/R-spondin1 rescues liver dysfunction through the induction of Lgr5(+) liver stem cells. Nat Commun. 2017 Oct 27 [PMID: 29079780] (ICC/IF, Mouse) ICC/IF Mouse
Lo YH, Chung E, Li Z et al. Transcriptional Regulation by ATOH1 and its Target SPDEF in the Intestine. Cell Mol Gastroenterol Hepatol. 2017 Jan 01 [PMID: 28174757] (ChIP, Human) ChIP Human
Jain A, Beutel O, Ebell K et al. Diverting CERT-mediated ceramide transport to mitochondria triggers Bax-dependent apoptosis. J. Cell. Sci. Nov 25 2016 [PMID: 27888218] (Human) Human
Brunk K, Zhu M, Barenz F et al. Cep78 is a novel centriolar protein involved in Plk4-induced centriole overduplication. J. Cell. Sci. May 31 2016 [PMID: 27246242]
Magarin M, Pohl T, Lill A et al. Embryonic cardiomyocytes can orchestrate various cell protective mechanisms to survive mitochondrial stress. J. Mol. Cell. Cardiol. 2016 Apr 19 [PMID: 27106802] (IHC-P, Mouse) IHC-P Mouse
Reguera M, Bassil E, Tajima H et al. pH Regulation by NHX-Type Antiporters Is Required for Receptor-Mediated Protein Trafficking to the Vacuole in Arabidopsis Plant Cell 2015 Mar 31 [PMID: 25829439] (WB) WB
Kratz AS, Barenz F, Richter KT et al. Plk4-dependent phosphorylation of STIL is required for centriole duplication Biol Open. 2015 Feb 20 [PMID: 25701666]
Wang S, Blumwald E. Stress-Induced Chloroplast Degradation in Arabidopsis Is Regulated via a Process Independent of Autophagy and Senescence-Associated Vacuoles. Plant Cell. 2014 Dec 23 [PMID: 25538186] (EM, Plant) EM Plant
Baumann U, Fernandez-Saiz V, Rudelius M et al. Disruption of the PRKCD-FBXO25-HAX-1 axis attenuates the apoptotic response and drives lymphomagenesis. Nat. Med. 2014 Nov 24 [PMID: 25419709]
Hammaren MM, Oksanen KE, Nisula HM et al. Adequate th2-type response associates with restricted bacterial growth in latent mycobacterial infection of zebrafish. PLoS Pathog. 2014 Jun 01 [PMID: 24968056] (WB)

Details:
Proteins were extracted from lck:GFP transgenic fish using TRI reagent - protein precipitation method (organs homogenized in 1.5 ml of TRI reagent ) and the protein pellet was solubilized in 0.1 ml of 1% SDS per 10 mg of tissue sample. Protein was loaded on 10% SDS-PAGE gel with 40ug/lane and WB was performed using anti-GFP antibody NB600-303 (Novus biological, Colorado, USA).
WB
Show All 42 Publications.

Reviews for GFP Antibody (NB600-303) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Other.
We have 3 reviews tested in 2 application: WB, IP.

Reviews using NB600-303:
Filter by Applications
WB
(2)
IP
(1)
All Applications
Filter by Species
Human
(2)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot GFP NB600-303
Enlarge
reviewed by:
Anonymous
WB Human 11/18/2011
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
CommentsThe antibody worked very well in western blot experiments.
Pub Med ID21777220

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:2500

Details

Detection NotesDetection Method: Lumilight (Roche)

Comments

CommentsThe antibody worked very well in western blot experiments.
Immunoprecipitation GFP NB600-303
Enlarge
reviewed by:
Marisa Carbonaro
IP Human 09/27/2010
View

Summary

ApplicationImmunoprecipitation
Sample TestedHeLa cells
SpeciesHuman
CommentsWorks very well for IP of GFP-tagged proteins. Got very clean results. Lysate Amount: 1mg

Comments

CommentsWorks very well for IP of GFP-tagged proteins. Got very clean results. Lysate Amount: 1mg
 
reviewed by:
Anonymous
WB Other 03/02/2009
View

Summary

ApplicationWestern Blot
Sample Testedrecombinant protein, Sample Amount: 0.5 - 1 ug
SpeciesOther
Lot21194
CommentsAll my samples have been treated with DNase - it appears in both the sample and the negative control.

Blocking

Blocking DetailsBlocking Buffer: PBS, 5% milk, Blocking Time: 20 minutes, Blocking Temp: 20 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/2500, Incubation Dilution Buffer: PBS+3% dry milk, Incubation Time: 90min, Incubation Temp: 20C

Secondary Antibody

Secondary DescriptionSecondary Ab: pAb goat anti-rabbit IgG, conj. AP, Secondary Ab Dilution Ratio: 1/10000
Secondary Manufacturer Cat#NB730-AP

Details

Detection NotesDetection Method:AP reaction, Exposure Time:10-30min, Neg Ctrl: sample w/o expressed protein, Non-Specific Band:~35kDa

Comments

CommentsAll my samples have been treated with DNase - it appears in both the sample and the negative control.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for GFP Antibody (NB600-303). (Showing 1 - 3 of 3 FAQs).

  1. I would like to know if ab290 from Abcam is identical to NB600-303?
    • Both antibodies (i.e. # ab290 from Abcam and # NB600-303 from Novus) use the same immunogen to develop this antibody. Both companies used highly purified recombinant full length protein made in E. coli as immunogen and this antibody is directed against the entire GFP molecule. We stand behind our products 100% and would be there to help in case you comes up with any concerns. Moreover our antibody is also well tested and has been cited in recent research papers in journals of high repute (Dis Model Mech. 2011 May;4(3):359-67; PLoS ONE. 2011; J Cell Sci. 2010 May 15;123(Pt 10):1732-41; Acta Anat (Basel). 1991;141(1):82-4; FASEB J. 2009 Nov 25. and many more...).
  2. Can you tell me the concentration for the latest lot of product NB600-303.
    • The current lot (31271) of NB600-303 is provided at a concentration of 6.05mg/ml.
  3. Does this antibody also detect ECFP?
    • NB600-303 has been validated for detection of S65T-GFP, RS-GFP, YFP and EGFP. Based on an alignment to a ECFP sequence that I found on NCBI (ncbi site), the GFP protein this product was raised against does show a good amount of homology. Therefore, there would be a good chance for cross reaction of this product against ECFP.

Secondary Antibodies

 

Isotype Controls

Additional GFP Products

Bioinformatics Tool for GFP Antibody (NB600-303)

Discover related pathways, diseases and genes to GFP Antibody (NB600-303). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Research Areas for GFP Antibody (NB600-303)

Find related products by research area.

Blogs on GFP.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

GFP - Be Green!
Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

GFP (Green Fluorescent Protein) Bringing Light to Life
GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
1
4
1
3
1
2
0
1
0

Anonymous
11/18/2011
Application: WB
Species: Human

Marisa Carbonaro
09/27/2010
Application: IP
Species: Human

 
Anonymous
03/02/2009
Application: WB
Species: Other

Bioinformatics

Gene Symbol GFP