This GFP antibody was developed against green fluorescent protein (GFP) from jellyfish
This GFP antibody recognizes green fluorescent protein (GFP), a 27 kDa protein which is derived from the jellyfish Aequorea victoria. GFP fluoresces green (509 nm) when excited by blue light (395 nm) and is commonly used as a marker of gene expression.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Drosophila reactivity reported in scientific literature (PMID: 26928065). This is a recombinantly expressed protein used in Drosphilia.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.09% Sodium Azide
Protein G purified
Alternate Names for GFP Antibody
Enhanced Green Fluorescent Protein
green fluorescent protein (gfp)
Green Fluorescent Protein
Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).
References 1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T.,... Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001
2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075
3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S.,... Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for GFP Antibody (NB100-62622)(5)
We have publications tested in 2 confirmed species: Mouse, Drosophila.
We have publications tested in 2 applications: ICC/IF, IHC-P.
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Showing Publications 1 - 5 of 5.
Publications using NB100-62622
Fujimoto TN, Colbert LE, Huang Y et al. Transcription of a plasticity gene is activated by neuronal hyperpolarization bioRxiv (ICC/IF, Drosophila)
Animal Models to Study Autophagy By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the... Read full blog post.
GFP - Be Green! Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v... Read full blog post.
Epitope Tags and the V5 Tag Antibody We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r... Read full blog post.
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