GFP Antibody

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB600-308] - Analysis of GFP in transgenic mouse pancreas in OCT. The blue region in the center is an islet which does not express GFP and is negative. Image from verified customer review.

Western Blot: GFP Antibody [NB600-308] - analysis of GFP tagged PKA subunits over-expressed in HEK293T cells using GFP antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: GFP Antibody [NB600-308] - Staining of GFP+ hepatocytes (brown) transplanted into the liver of a recipient animal using anti-GFP antibody. Cyclin D1 double staining in blue. Image from verified customer review.

Western Blot: GFP Antibody [NB600-308] - Western Blot of GFP antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: CHO/K1 Lysate. Lane 4: MDA-MB-231. Lane 5: A431 Lysate. Lane 6: Jurkat Lysate. Lane 7: NIH/3T3 Lysate. Lane 8: E-coli HCP Control. Lane 9: FLAG Positive Control Lysate. Lane 10: Red Fluorescent Protein. Lane 11: Green Fluorescent Protein. Lane 12: Glutathione-S-Transferase Protein. Lane 13: Maltose Binding Protein. Load: 10 ug of lysate or 50ng of purified protein per lane. Primary antibody: GFP antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted/Observed size: 30 kDa for GFP.

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB600-308]

Immunohistochemistry: GFP Antibody [NB600-308] - Analysis of GFP in mouse retinal cells. Image courtesy of anonymous customer review.

Western Blot: GFP Antibody [NB600-308] - Western Blot of GFP antibody. Lane 1: Wild type GFP (0.1 ug) was used to spike HeLa whole cell lysate. Lane 2: none. Load: 30 ug per lane.Primary antibody: GFP antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) Goat-a-Rabbit IgG [H&L] MX10 at 1:10,000 for 45 min at RT.Block: 5% BLOTTO in PBS overnight at 4C.Predicted/Observed size: 27 kDa for epitope tag GFP. Other band(s): none.

Western Blot: GFP Antibody [NB600-308] - Western Blot of GFP antibody. Lane 1: 293FT cells transfected with CDK4 dominant negative (C-).Lane 2: 293FT cells poitive control (C+).Load: 25 ug per lane.Primary antibody: GFP antibody at 1:400 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 27 kDa for GFP.

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB600-308] - Immuno-microscopy of GFP antibody. Monocyte derived dendritic cells and dermal macrophages were challenged and directly visualized with eGFP labeled Dengue virus to localize sequestration of virus particles in the different cells (upper). The location of the GFP was confirmed by TEM (lower magnified view) using rabbit anti GFP Primary antibody (1:200) and a gold labeled secondary antibody.

Immunohistochemistry-Frozen: GFP Antibody [NB600-308] - Adult mouse brain tissue. This image was submitted via customer review.

Immunoprecipitation: GFP Antibody [NB600-308] - IP analysis of GFP in S2 Cells (Drosophila). Image from verified customer review.

Immunoprecipitation: GFP Antibody [NB600-308] - Immunoprecipitation experiments showing that GFP-MISP binds endogenous IQGAP1 (upper panel) and MISP binds to IQGAP1 endogenously using specific antibodies (lower panel) in mitotically arrested (taxol and nocodazole, respectively) HeLa cell lysates. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24682-8), licensed under a CC-BY licence.

• Reactivity: All-NA
• Applications: WB, DB, ELISA, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl, IHC-WhMt, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GFP Antibody Summary

• Immunogen: The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
• Specificity: Anti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). Primate reactivity reported in scientific literature (PMID: 27723741).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Electron Microscopy 1:10-1:500
  • ELISA 1:20000-1:120000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:500-1:5000
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:200-1:3000
  • Immunohistochemistry-Frozen 1:50-1:250
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:500-1:5000
• Application Notes: Use in Immunoprecipitation reported in scientific literature (PMID:34887587).Use in DB reported in scientific literature (PMID:34242364). This antibody is designed to detect GFP and its variants. GFP antibody can be used to detect GFP by ELISA (sandwich or capture). for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. This Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. This antibody can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli). and eukaryotic (CHO cells). expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
  Use in Knockdown Validated reported in scientific literature (PMID: 32905777)...
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

No reaction was observed against Human, Mouse serum proteins. Suitable for detecting fusion proteins containing the GFP sequence expressed in Human, Mouse, Rat, C. elegans, Drosophila and in vitro transcription/translation systems and transgenic animals. GFP Transgenic Rat reactivity reported in scientific literature (PMID:25724725). Mouse reactivity reported in multiple pieces of scientific literature.Transgenic C. elegans reactivity reported in scientific literature (PMID: 27110099). Known cross reactivity with wt and all variants such as rGFP, eGFP, S65T-GFP, RS-GFP, YFP and EGFP. Use in Mouse reported in secitific publication PMID: 32765228 Plant reactivity reported in scientific literature (PMID:32896843)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria)

GFP antibody is stable for several weeks at 4C as an undiluted liquid.

Alternate Names for GFP Antibody

• anti-gfp (green fluorescent protein) pab
• eGFP
• Enhanced Green Fluorescent Protein
• GFP immunofluorescence
• GFP immunoprecipitation
• GFP mouse
• GFP staining
• GFP western blot
• GFP
• GFPuv
• green fluorescent protein (gfp)
• Green Fluorescent Protein

Background

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T., . . . Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S., . . . Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB600-308)(223)

Publications using NB600-308

• Osaki Y, Manolopoulou M, Ivanova AV et al. Blocking cell cycle progression through CDK4/6 protects against chronic kidney disease JCI insight Jun 22 2022 [PMID: 35730565] (Flow, IHC)
• Feng C, Wang Y, Zha X et al. Cold-sensitive ventromedial hypothalamic neurons control homeostatic thermogenesis and social interaction-associated hyperthermia Cell metabolism Jun 7 2022 [PMID: 35675799] (IHC)
• Ma L, Chen C, Liu D et al. Apoptotic extracellular vesicles are metabolized regulators nurturing the skin and hair Bioactive materials Jan 1 2023 [PMID: 35600968] (ICC/IF)
• Law SSY, Liou G, Nagai Y et al. Polymer-coated carbon nanotube hybrids with functional peptides for gene delivery into plant mitochondria Nature communications May 16 2022 [PMID: 35577779] (WB)
• Gu T, Hao D, Woo J et al. The disordered N-terminal domain of DNMT3A recognizes H2AK119ub and is required for postnatal development Nature genetics May 1 2022 [PMID: 35534561] (WB)
• Lao M, Hurtado A, de Castro AC et al. Sox9 is required for nail bed differentiation and digit tip regeneration The Journal of investigative dermatology Apr 7 2022 [PMID: 35398376]
• Thagun C, Horii y, Mori M et al. Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts ACS nano Feb 23 2022 [PMID: 35195009] (WB)
• Kakoty V, C SK, Yang CH et al. Neuroprotective Effect of Lentivirus-Mediated FGF21 Gene Delivery in Experimental Alzheimer's Disease is Augmented when Concerted with Rapamycin Molecular neurobiology Feb 10 2022 [PMID: 35142986]
• Elbaz M, Callado Perez A, Demers M et al. A vibrissa pathway that activates the limbic system eLife Feb 10 2022 [PMID: 35142608] (IHC)
• Sun J, Yoon J, Lee M et al. Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development Cell reports Feb 1 2022 [PMID: 35108539] (ICC/IF)
• Osokine I, Siewiera J, Rideaux D et al. Gene silencing by EZH2 suppresses TGF-beta activity within the decidua to avert pregnancy-adverse wound healing at the maternal-fetal interface Cell reports Feb 1 2022 [PMID: 35108527]
• Chalif JI, MartInez-Silva ML, Pagiazitis JG et al. Control of mammalian locomotion by ventral spinocerebellar tract neurons Cell Jan 20 2022 [PMID: 35063074] (IHC-P)
• Wu Y, Devotta A, JosE-Edwards DS et al. Xbp1 and Brachyury establish an evolutionarily conserved subcircuit of the notochord gene regulatory network eLife Jan 20 2022 [PMID: 35049502] (ISH)
• Chen B, Scurrah CR, McKinley ET et al. Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps Cell Dec 22 2021 [PMID: 34910928] (Flow)
• Ramos H, Monette A, Niu M et al. The double-stranded RNA-binding protein, Staufen1, is an IRES-transacting factor regulating HIV-1 cap-independent translation initiation Nucleic acids research Dec 6 2021 [PMID: 34893869] (WB)
• Shu XE, Mao Y, Jia L, Qian SB Dynamic eIF3a O-GlcNAcylation controls translation reinitiation during nutrient stress Nature chemical biology Dec 9 2021 [PMID: 34887587] (IP)
• Joshi B, Wagh G, Kaur H, Patra C Zebrafish Model to Study Angiotensin II-Mediated Pathophysiology Biology Nov 13 2021 [PMID: 34827169] (IHC)
• McGuire MR, Mukhopadhyay D, Myers SL Et al. Progesterone receptor membrane component 1 (PGRMC1) binds and stabilizes cytochromes P450 through a heme-independent mechanism The Journal of biological chemistry Oct 19 2021 [PMID: 34678314] (WB)
• Hausmann A, Felmy B, Kunz L et al. Intercrypt sentinel macrophages tune antibacterial NF- kappaB responses in gut epithelial cells via TNF The Journal of experimental medicine Nov 1 2021 [PMID: 34529751] (ICC/IF)
• Zhang W, Lanzoni G, Hani H Et al. Patch grafting, strategies for transplantation of organoids into solid organs such as liver Biomaterials Aug 1 2021 [PMID: 34517276] (ICC/IF)
• Kong JH, Young CB, Pusapati GV Et al. Gene-teratogen interactions influence the penetrance of birth defects by altering Hedgehog signaling strength Development (Cambridge, England) Oct 1 2021 [PMID: 34486668]
• Girish N, Liu CY, Gadeock S et al. Persistence of Lgr5+ colonic epithelial stem cells in mouse models of inflammatory bowel disease American journal of physiology. Gastrointestinal and liver physiology Jul 14 2021 [PMID: 34260310] (IHC-P)
• Bourgeois Js, Wang L, Rabino Af Et Al. ARHGEF26 enhances Salmonella invasion and inflammation in cells and mice PLoS pathogens Jul 1 2021 [PMID: 34242364] (DB)
• Singh B, Khattab F, Chae H et al. KATP channel blockers control glucagon secretion by distinct mechanisms: The direct stimulation of alpha-cells involving a [Ca2+]c rise and an indirect inhibition mediated by somatostatin Molecular metabolism Jun 9 2021 [PMID: 34118477] (IHC)
• Xu PF, Borges RM, Fillatre J et al. Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre Nature communications Jun 2 2021 [PMID: 34078907] (IHC)
• Bota-Rabassedas N, Banerjee P, Niu Y et al. Contextual cues from cancer cells govern cancer-associated fibroblast heterogeneity Cell reports Apr 20 2021 [PMID: 33882319]
• McElvain LE, Chen Y, Moore JD et al. Specific populations of basal ganglia output neurons target distinct brain stem areas while collateralizing throughout the diencephalon Neuron Apr 1 2021 [PMID: 33823137]
• Yu D, G Febbo I, J Maroteaux M et al. The Transcription Factor Shox2 Shapes Neuron Firing Properties and Suppresses Seizures by Regulation of Key Ion Channels in Thalamocortical Neurons Cerebral cortex (New York, N.Y. : 1991) Mar 2 2021 [PMID: 33675359] (IHC, WB)
• Oikawa K, Imai T, Thagun C et al. Mitochondrial movement during its association with chloroplasts in Arabidopsis thaliana Communications biology Mar 5 2021 [PMID: 33674706] (WB)
• Tang C, Han J, Dalvi S et al. A human model of Batten disease shows role of CLN3 in phagocytosis at the photoreceptor-RPE interface Commun Biol 2021 Feb 5 [PMID: 33547385] (WB)
• González-Prieto R, Eifler-Olivi K, Claessens LA, et al. Global non-covalent SUMO interaction networks reveal SUMO-dependent stabilization of the non-homologous end joining complex Cell reports Jan 26 2021 [PMID: 33503430]
• Bland KM, Aharon A, Widener EL, et al. FMRP regulates the subcellular distribution of cortical dendritic spine density in a non-cell-autonomous manner Neurobiology of disease Jan 6 2021 [PMID: 33421563]
• Vergarajauregui S, Becker R, Steffen U et al. AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9 eLife Dec 9 2020 [PMID: 33295871] (Non-species specific)
• Wolter JM, Mao H, Fragola G et al. Cas9 gene therapy for Angelman syndrome traps Ube3a-ATS long non-coding RNA Nature Oct 21 2020 [PMID: 33087932]
• Sade N, Weng F, Tajima H et al. A Cytoplasmic Receptor-like Kinase Contributes to Salinity Tolerance Plants (Basel) Oct 17 2020 [PMID: 33080797]
• Im JY, Kim DM, Park H et al. VGLL1 phosphorylation and activation promotes gastric cancer malignancy via TGF-B/ERK/RSK2 signaling Biochim Biophys Acta Mol Cell Res Oct 16 2020 [PMID: 33069758] (WB)
• Hamaoui, D, CossE, M M Et al. The Chlamydia effector CT622/TaiP targets a nonautophagy related function of ATG16L1. Proc Natl Acad Sci U S A Oct 27 2020 [PMID: 33055216] (IHC)
• Donato C, Kunz L, Castro-Giner F et al. Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells Cell reports Sep 8 2020 [PMID: 32905777] (WB, KD, ICC/IF)
• Thagun C , Motoda Y , Kigawa T et al. Simultaneous introduction of multiple biomacromolecules into plant cells using a cell-penetrating peptide nanocarrier Nanoscale 2020 Sep 28 [PMID: 32896843] (WB)
• Kim H, Berens NC, Ochandarena NE, Philpot BD Region and Cell Type Distribution of TCF4 in the Postnatal Mouse Brain Front Neuroanat 2020 Jul 17 [PMID: 32765228] (ICC/IF)
• Saha C, Mohanraju P, Stubbs A et al. Guide-free Cas9 from pathogenic Campylobacter jejuni bacteria causes severe damage to DNA Sci Adv Jun 1 2020 [PMID: 32596446] (WB)
• Cuijpers SAG, Willemstein E, Ruppert JG et al. Chromokinesin KIF4A teams up with Stathmin 1 to regulate abscission in a SUMO-dependent manner J. Cell. Sci. Jun 26 2020 [PMID: 32591481] (WB)
• Frantz MG, Crouse EC, Sokhadze G et al. Layer 4 Gates Plasticity in Visual Cortex Independent of a Canonical Microcircuit Curr. Biol. Jun 18 2020 [PMID: 32589913]
• Shin E Y, Park J H et al. Integrin-mediated adhesions in regulation of cellular senescence. Sci Adv Jan 5 2020 [PMID: 32494696] (IHC, WB)
• Min H, Youn E, Shim YH Maternal Caffeine Intake Disrupts Eggshell Integrity and Retards Larval Development by Reducing Yolk Production in a Caenorhabditis elegans Model Nutrients 2020 May 7 [PMID: 32392893] (WB)
• Chen Z, Wang ZH, Zhang G et al. Mitochondrial DNA segregation and replication restrict the transmission of detrimental mutation J. Cell Biol. Jul 6 2020 [PMID: 32375181] (ICC/IF)
• Taniguchi T, Zanetti-Yabur A, Wang P et al. Interindividual Diversity in Expression of Organic Anion Uptake Transporters in Normal and Cirrhotic Human Liver Hepatol Commun May 5 2020 [PMID: 32363323] (WB)
• Monette A, Niu M, Chen L et al. Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking Cell Rep Apr 23 2020 [PMID: 32320662] (WB)
• Steinberg EE, Gore F, Heifets BD et al. Amygdala-Midbrain Connections Modulate Appetitive and Aversive Learning Neuron Apr 16 2020 [PMID: 32294466]
• Kashiwagi H, Ishimoto H, Izumi SI et al. Human PZP and common marmoset A2ML1 as pregnancy related proteins Sci Rep Mar 22 2020 [PMID: 32198464] (IHC)
• Hu X, Deng Q, Ma L et al. Meningeal lymphatic vessels regulate brain tumor drainage and immunity Cell Res Feb 26 2020 [PMID: 32094452] (IHC-Fr)
• Tsukamoto T, Gearhart M D et al. Insights into the Involvement of Spliceosomal Mutations in Myelodysplastic Disorders from Analysis of SACY-1/DDX41 in Caenorhabditis elegans. Genetics Jan 4 2020 [PMID: 32060018] (WB)
• Corbet C, Bastien E, Santiago de Jesus JP et al. TGFbeta2-induced formation of lipid droplets supports acidosis-driven EMT and the metastatic spreading of cancer cells Nat Commun 2020 Jan 23 [PMID: 31974393] (IHC)
• Dubey R, Stivala CE, Nguyen HQ et al. Lipid droplets can promote drug accumulation and activation Nat. Chem. Biol. Feb 1 2020 [PMID: 31932720] (WB)
• Thagun C, Chuah JA, Numata K. Targeted Gene Delivery into Various Plastids Mediated by Clustered Cell-Penetrating and Chloroplast-Targeting Peptides Adv Sci (Weinh) Dec 14 2019 [PMID: 31832328] (IP, WB)
• Kim B K, Cheong J H et al. PI3K/AKT/beta-Catenin Signaling Regulates Vestigial-Like 1 Which Predicts Poor Prognosis and Enhances Malignant Phenotype in Gastric Cancer. Cancers (Basel) Mar 12 2019 [PMID: 31816819] (WB)
• Kunz L, Schroeder T A 3D Tissue-wide Digital Imaging Pipeline for Quantitation of Secreted Molecules Shows Absence of CXCL12 Gradients in Bone Marrow Cell Stem Cell 2019 Dec 5 [PMID: 31809740] (ICC/IF)
• Wang Z H, Liu Y et al. Electron transport chain biogenesis activated by a JNK-insulin-Myc relay primes mitochondrial inheritance in Drosophila. Elife Oct 15 2019 [PMID: 31612862] (IHC)
• Mercer Lindsay N, Knutsen PM, Lozada AF et al. Orofacial Movements Involve Parallel Corticobulbar Projections from Motor Cortex to Trigeminal Premotor Nuclei Neuron 2019 Sep 20 [PMID: 31587918]
• Gewin L, Summers ME, Harral JW et al. Inactivation of Tsc2 in Abcg2 Lineage Derived Cells Drives the Appearance of Polycystic Lesions and Fibrosis in the Adult Kidney Am. J. Physiol. Renal Physiol. Aug 28 2019 [PMID: 31461347]
• Guo B, Itami J et al. Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides. PLoS One Jul 31 2019 [PMID: 31361745] (WB)
• Zusso M, Lunardi V, Franceschini D et al. Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway J Neuroinflammation 2019 Jul 18 [PMID: 31319868] (WB)
• Shi C, Pan FC, Kim JN et al. Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis Cell Mol Gastroenterol Hepatol 2019 [PMID: 31310834] (IHC)
• English JG, Olsen RHJ, Lansu K et al. VEGAS as a Platform for Facile Directed Evolution in Mammalian Cells Cell Jul 25 2019 [PMID: 31280962]
• Lee M, Hwang Y S et al. Developmentally regulated GTP-binding protein 1 modulates ciliogenesis via an interaction with Dishevelled. J Cell Biol Mar 7 2019 [PMID: 31270137] (IHC, IP)
• Nilangekar K, Murmu N, Sahu G et al. Generation and Characterization of Germline-Specific Autophagy and Mitochondrial Reactive Oxygen Species Reporters in Drosophila Front Cell Dev Biol Apr 20 2019 [PMID: 31001531] (WB)
• Cruz-Ortega JS, Boucard AA. Actin cytoskeleton remodeling defines a distinct cellular function for adhesion G protein-coupled receptors ADGRL/latrophilins 1, 2 and 3 Biol Open Mar 31 2019 [PMID: 30926595] (WB)
• Sandhu R, Wei D, Sharma M et al. An N-terminal Flag-tag impairs TPP1 regulation of telomerase function Biochem Biophys Res Commun Mar 20 2019 [PMID: 30885434] (WB)
• Li A, Cho JH, Reid B et al. Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks Nat Commun Dec 19 2018 [PMID: 30560870] (IHC)
• Di Scala C, Tessier M, Sapet C et al. A new polymer-based approach for in vivo transfection in postnatal brain. J Neurosci Methods 2019 Jan 1 [PMID: 30408559]
• Li Q, Saito TT, Martinez-Garcia M et al. The tumor suppressor BRCA1-BARD1 complex localizes to the synaptonemal complex and regulates recombination under meiotic dysfunction in Caenorhabditis elegans PLoS Genet Nov 2 2018 [PMID: 30383767] (WB)
• Zhao S, Fortier TM, Baehrecke EH. Autophagy Promotes Tumor-like Stem Cell Niche Occupancy Curr Biol Oct 2 2018 [PMID: 30270184] (ICC/IF)
• Cain NE, Jahed Z, Schoenhofen A et al. Conserved SUN-KASH Interfaces Mediate LINC Complex-Dependent Nuclear Movement and Positioning Curr Biol Sep 25 2018 [PMID: 30245107] (ICC/IF)
• Spike CA, Huelgas-Morales G, Tsukamoto T et al. Multiple Mechanisms Inactivate the LIN-41 RNA-Binding Protein To Ensure a Robust Oocyte-to-Embryo Transition in Caenorhabditis elegans Genetics Sep 13 2018 [PMID: 30206186] (WB)
• Wang L, Chen SR, Ma H et al. Regulating nociceptive transmission by VGluT2-expressing spinal dorsal horn neurons J Neurochem 2018 Nov [PMID: 30203849] (IHC)
• Lee HM, Clark EP, Kuijer MB et al. Characterization and structure-activity relationships of indenoisoquinoline-derived topoisomerase I inhibitors in unsilencing the dormant Ube3a gene associated with Angelman syndrome Mol Autism 2018 Aug 17 [PMID: 30140420] (ICC/IF)
• Yao H, Price TT, Cantelli G et al. Leukaemia hijacks a neural mechanism to invade the central nervous system Nature Jul 20 2018 [PMID: 30022166] (IHC)
• Nakano Y, Kelly MC, Rehman AU et al. Defects in the Alternative Splicing-Dependent Regulation of REST Cause Deafness Cell Jul 3 2018 [PMID: 29961578] (ICC/IF)
• Beer-Hammer S, Lee SC, Mauriac SA et al. Gai Proteins are Indispensable for Hearing. Cell. Physiol. Biochem. Jun 21 2018 [PMID: 29940568] (IHC)
• Stephany CE, Ma X, Dorton HM et al. Distinct Circuits for Recovery of Eye Dominance and Acuity in Murine Amblyopia Curr Biol Jun 12 2018 [PMID: 29887305]
• Bell P, Yu H, Kuntz L et al. Preparation of Nonhuman Primate Eyes for Histological Evaluation After Retinal Gene Transfer Hum Gene Ther Methods Jun 1 2018 [PMID: 29848071] (IHC)
• Du P, Pirouz M, Choi J et al. An Intermediate Pluripotent State Controlled by MicroRNAs Is Required for the Naive-to-Primed Stem Cell Transition Cell Stem Cell May 16 2018 [PMID: 29804889] (IHC)
• Herrera LA, Starr DA The E3 Ubiquitin Ligase MIB-1 Is Necessary To Form the Nuclear Halo in Caenorhabditis elegans Sperm G3 (Bethesda) 2018 Jul 2 [PMID: 29776970] (IHC)
• Shi B, Xue M, Wang Y et al. An improved method for increasing the efficiency of gene transfection and transduction Int J Physiol Pathophysiol Pharmacol May 15 2018 [PMID: 29755642] (ICC/IF)
• Furuta T, Joo HJ, Trimmer KA et al. GSK-3 promotes S-phase entry and progression in C. elegans germline stem cells to maintain tissue output Development Apr 27 2018 [PMID: 29695611]
• Vodicska B, Cerikan B, Schiebel E et al. MISP regulates the IQGAP1/Cdc42 complex to collectively orchestrate spindle orientation and mitotic progression Sci Rep Apr 22 2018 [PMID: 29679050] (ICC/IF, WB)
• O'Neill S, Mathis M, Kovacic L et al. Quantitative interaction analysis permits molecular insights into functional NOX4 NADPH oxidase heterodimer assembly J Biol Chem Apr 21 2018 [PMID: 29674345] (IP)
• Lebeau P, Platko K, Al-Hashimi AA et al. Loss-of-function PCSK9 mutants evade the unfolded protein response sensor GRP78 and fail to induce endoplasmic reticulum stress when retained J Biol Chem 2018 May 11 [PMID: 29593095]
• Finn JD, Smith AR, Patel MC et al. A Single Administration of CRISPR/Cas9 Lipid Nanoparticles Achieves Robust and Persistent In Vivo Genome Editing Cell Rep Mar 1 2018 [PMID: 29490262]
• Dell'Aringa M, Reinhardt RL. Notch signaling represents an important checkpoint between follicular T-helper and canonical T-helper 2 cell fate Mucosal Immunol Feb 23 2018 [PMID: 29467447] (ICC/IF)
• Wang A, Conicella AE, Schmidt HB, Martin EW. A single N-terminal phosphomimic disrupts TDP-43 polymerization, phase separation, and RNA splicing. EMBO J. 2018 Feb 09 [PMID: 29438978] (WB)
• Pusapati GV, Kong JH, Patel BB et al. G protein-coupled receptors control the sensitivity of cells to the morphogen Sonic Hedgehog Sci Signal Feb 14 2018 [PMID: 29438014]
• Linden AG, Li S, Choi HY et al. Interplay between ChREBP and SREBP-1c coordinates postprandial glycolysis and lipogenesis in livers of mice J Lipid Res 2018 [PMID: 29335275] (WB)
• Buzgariu W, Wenger Y, Tcaciuc N et al. Impact of cycling cells and cell cycle regulation on Hydra regeneration Dev Biol Jan 3 2018 [PMID: 29291976] (ICC/IF)
• Brumbaugh J, Di Stefano B, Wang X et al. Nudt21 Controls Cell Fate by Connecting Alternative Polyadenylation to Chromatin Signaling Cell Dec 19 2017 [PMID: 29249356]
• Herring CA, Banerjee A, McKinley ET et al. Unsupervised Trajectory Analysis of Single-Cell RNA-Seq and Imaging Data Reveals Alternative Tuft Cell Origins in the Gut Cell Syst 2018 Jan 24 [PMID: 29153838]
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• Knutson AK, Egelhofer T, Rechtsteiner A, Strome S Germ Granules Prevent Accumulation of Somatic Transcripts in the Adult Caenorhabditis elegans Germline Genetics 2017 [PMID: 28258184] (ICC/IF)
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• Yovchev MI, Oertel M. Fetal Liver Stem/Progenitor Cell Transplantation: A Model to Study Tissue Mass Replacement and Cell-Based Therapies. Methods Mol. Biol. Nov 10 2016 [PMID: 27830548]
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• Bao K, Carr T, Wu J et al. BATF Modulates the Th2 Locus Control Region and Regulates CD4+ T Cell Fate during Antihelminth Immunity J Immunol Nov 1 2016 [PMID: 27798167] (IHC)
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• Davidson R, Laporte D, Wu JQ. Regulation of Rho GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis. Mol. Biol. Cell. 2014 Dec 03 [PMID: 25473118] (IP)
  
  Details:
  GFP antibody used in IP for pulling down the YFP-tagged protein expressed at its native level from fission yeast cell extracts (Figure 3E)
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  Details:
  GFP antibody used for immunofluorescence staining of C elegans embryos - antibody diluted 1:200 in UNC-83 antiserum for simultaneous staining of KASH and SUN chimeric proteins.
• Shigunov Patricia, Sotelo-Silveira Jose, Stimamiglio Marco Augusto et al. Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules. BMC Mol Biol. 2014 Jul 03 [PMID: 24993635] (IP)
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• Tian G, Ropelewski P, Nemet I et al. An unconventional secretory pathway mediates the Cilia targeting of peripherin/rds. J. Neurosci. 2014 Jan 15 [PMID: 24431457] (WB)
• Nachtergaele S, Whalen DM, Mydock LK et al. Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling. Elife. 2013 Oct 29 [PMID: 24171105] (WB)
• Oldenbroek M, Robertson SM, Guven-Ozkan T et al. Regulation of maternal Wnt mRNA translation in C. elegans embryos. Development. 2013 Nov [PMID: 24131629] (IP)
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• Powell AE, Wang Y, Li Y et al. The pan-ErbB negative regulator Lrig1 is an intestinal stem cell marker that functions as a tumor suppressor Cell 2012 Mar 30 [PMID: 22464327] (IHC-Fr, ICC/IF)
• Lee IJ, Wu JQ. Characterization of Mid1 domains for targeting and scaffolding in fission yeast cytokinesis. J Cell Sci 2012 Jun 15 [PMID: 22427686] (WB)
• Ahmed M, Wong EY, Sun J et al. Eya1-six1 interaction is sufficient to induce hair cell fate in the cochlea by activating atoh1 expression in cooperation with sox2. Dev Cell;22(2):377-90. 2012 Feb 14. [PMID: 22340499]
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• Damulewicz M, Pyza E. The clock input to the first optic neuropil of Drosophila melanogaster expressing neuronal circadian plasticity. PLoS One 2011 [PMID: 21760878]
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• Chen X, Nelson CD, Li X et al. PSD-95 is required to sustain the molecular organization of the postsynaptic density J Neurosci Apr 29 2011 [PMID: 21525273]
• Laporte D, Coffman VC, Lee IJ, Wu JQ. Assembly and architecture of precursor nodes during fission yeast cytokinesis. J Cell Biol 2011 Mar 21 [PMID: 21422229] (IP)
• Ray KC, Bell KM, Yan J et al. Epithelial tissues have varying degrees of susceptibility to Kras(G12D)-initiated tumorigenesis in a mouse model PLoS One 2011 Feb 2 [PMID: 21311774] (IHC)
• Sparks EE, Perrien DS, Huppert KA et al. Defects in hepatic Notch signaling result in disruption of the communicating intrahepatic bile duct network in mice Dis Model Mech 2011 [PMID: 21282722] (IHC, ICC/IF)
• Torres J, Funk HM, Zegers MM et al. The syntaxin 4 N terminus regulates its basolateral targeting by munc18c-dependent and -independent mechanisms. J Biol Chem 2011 Mar [PMID: 21278252]
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• Fridolfsson HN, Starr DA. Kinesin-1 and dynein at the nuclear envelope mediate the bidirectional migrations of nuclei. J Cell Biol 2010 Oct 4 [PMID: 20921138] (ICC/IF)
• Xue X, Jaulin F, Espenel C, Kreitzer G. PH-domain-dependent selective transport of p75 by kinesin-3 family motors in non-polarized MDCK cells J Cell Sci 2010 May 15 [PMID: 20427314] (IP)
• Jaramillo-Lambert A, Engebrecht J. A Single Unpaired and Transcriptionally Silenced X Chromosome Locally Precludes Checkpoint Signaling in the Caenorhabditis elegans Germ Line. Genetics. [PMID: 20008570]
• Fridolfsson HN, Ly N, Meyerzon M, Starr DA. UNC-83 coordinates kinesin-1 and dynein activities at the nuclear envelope during nuclear migration. Dev Biol 2010 Feb 15 [PMID: 20005871] (WB, ICC/IF)
• Hoffman GE, Le WW, Sita LV. The importance of titrating antibodies for immunocytochemical methods. Curr Protoc Neurosci. 2008 Oct [PMID: 18972376]
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• Shafikhani, HS, Engel, Joanne. Pseudomonas aeruginosa type III-secreted toxin ExoT inhibits host-cell division by targeting cytokinesis at multiple steps. PNAS 103:15605-15610. 2006 [PMID: 17030800]
• Moon H, Zhu J, Donahue LR et al. Electron transport chain biogenesis activated by a JNK-insulin-Myc relay primes mitochondrial inheritance in Drosophila bioRxiv May 18 2019 (IHC, Drosophila)
• Steeg C, Adler G, Sparwasser T et al. Limited role of CD4+Foxp3+ regulatory T cells in the control of experimental cerebral malaria. J Immunol;183(11):7014-22. 2009 Dec 1. [PMID: 19890049]
• Dindot, SV et al. The Angelman syndrome ubiquitin ligase localizes to the synapse nucleus, maternal deficiency results in abnormal dendritic spine morphology. Hum Mol Genet 17(1): 111-118. 2008 [PMID: 17940072]
• Tretyakova I, Zolotukhin AS, Tan W et al. Nuclear export factor family protein participates in cytoplasmic mRNA trafficking. J Biol Chem 2005 Sep 9 [PMID: 16014633]
• Day, Y-J et al. A2a Adenosine receptors on bone marrow-derived cells protect liver from ischemia-reperfusion injury. J Immunol 174:5040-5046. 2005 [PMID: 15814735]
• Bowman AB, Yoo SY, Dantuma NP et al. Neuronal dysfunction in a polyglutamine disease model occurs in the absence of ubiquitin-proteasome system impairment and inversely correlates with the degree of nuclear inclusion formation. Hum Mol Genet;14(5):679-91. 2005 Mar 1. [PMID: 15661755]
• Merkle, FT et al. Radial glia give rise to adult neural stem cells in the subventricular zone. PNAS 101:17528-17532. 2004 [PMID: 15574494]
• Kanazawa, Y, Verma, IM. Little evidence of bone moarrow-derived hepatocytes in the replacement of injured liver. PNAS 100:11850-11853. 2003 [PMID: 12920184]
• Kootstra, NA et al. Abrogation of postentry resctriction of HIV-1-based lentiviral vector transduction in simian cells. PNAS 100:1298-1303. 2003 [PMID: 12547912]
• Meyerzon M, Fridolfsson HN, Ly N et al. UNC-83 is a nuclear-specific cargo adaptor for kinesin-1-mediated nuclear migration. Development;136(16):2725-2733. 2009 [PMID: 9605495]
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• Autry AE, Wu Z, Kohl J, Bambah-Mukku D Perifornical Area Urocortin-3 Neurons Promote Infant-directed Neglect and Aggression bioRxiv Jul 9 2019 (IHC, Mouse)
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• Chowdhury, A Tamalin/GRAS-1 Connects Glutamate Receptor Activity to the Insulin/IGF Signaling Cascade to Regulate Neuroprotection in a Nematode Model of Excitotoxicity Thesis Jan 1 2020 (IP, C. elegans)
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Reviews for GFP Antibody (NB600-308) (18)

Average Rating: 4.5

(Based on 18 reviews)

We have 18 reviews tested in 4 species: Human, Mouse, Rat, Other.

reviewed by: Abhijit Nirwane Immunohistochemistry-Frozen Mouse 04/12/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Adult mouse brain tissue
• Species: Mouse
• Lot: 35459

Comments

• Comments: Dilutions 1:400

reviewed by: Priyanka Manandhar Immunocytochemistry Mouse 03/22/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: FFPE
• Species: Mouse

Comments

• Comments: Trypsin retrieval

reviewed by: Verified Customer Western Blot Human 03/10/2017

Summary

• Application: Western Blot
• Sample Tested: whole cellular protein and fractionated (soluble and membrane protein)
• Species: Human

Comments

• Comments: Human embryonic kidney 293T
  HEK293T cells

reviewed by: Verified Customer Immunofluorescence on tissues. Human 03/02/2017

Summary

• Application: Other
• Sample Tested: Human Xenograft Tumor
• Species: Human

reviewed by: Anonymous - Immunofluorescence Other 07/27/2016

Summary

• Application: Immunofluorescence
• Sample Tested: Pig TM cells
• Species: Other

reviewed by: Ganesh Pusapati Immunofluorescence Mouse 10/10/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse, 3T3 cells
• Species: Mouse
• Lot: 28839

reviewed by: Ganesh Pusapati Western Blot Human 10/10/2015

Summary

• Application: Western Blot
• Sample Tested: HEK293T whole cell extract
• Species: Human
• Lot: 28839

reviewed by: Marcio Fontenele Immunoprecipitation Other 04/02/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: S2 Cells (Drosophila)
• Species: Other

reviewed by: Verified Customer Immunofluorescence Mouse 11/25/2014

Summary

• Application: Immunofluorescence
• Sample Tested: transgenic mouse pancreas
• Species: Mouse

reviewed by: Fabio Marongiu Immunohistochemistry-Paraffin 10/05/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Lot: 28839

reviewed by: Verified Customer Immunohistochemistry-Frozen Mouse 11/06/2013

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Transgenic mouse pancreas in OCT
• Species: Mouse
• Lot: 24437

reviewed by: Verified Customer Immunofluorescence Rat 07/13/2012

Summary

• Application: Immunofluorescence
• Sample Tested: Rat liver
• Species: Rat
• Lot: 24437

reviewed by: Verified Customer Immunoprecipitation Human 04/24/2012

Summary

• Application: Immunoprecipitation
• Sample Tested: Hela whole cell lysate
• Species: Human

reviewed by: Verified Customer Western Blot Other 11/15/2011

Summary

• Application: Western Blot
• Sample Tested: Yeast
• Species: Other

reviewed by: Anna Means Immunohistochemistry Mouse 09/28/2011

Summary

• Application: Immunohistochemistry
• Sample Tested: IHC on parrafin sections (mouse)
• Species: Mouse

reviewed by: Verified Customer Immunofluorescence Mouse 07/20/2011

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse Retinal Cells
• Species: Mouse

reviewed by: Verified Customer Western Blot Other 11/23/2010

Summary

• Application: Western Blot
• Sample Tested: Pig ear fibroblasts, Sample Amount: 10 ul
• Species: Other

reviewed by: Verified Customer Western Blot Mouse 09/23/2010

Summary

• Application: Western Blot
• Sample Tested: P19 cells transfectedwith a plasmid DNA expressing a GFP fusion protein, Sample Amount: 20 micrograms
• Species: Mouse

FAQs for GFP Antibody (NB600-308). (Showing 1 - 2 of 2 FAQs).

1. Does the GFP antibody NB600-308 also work against AcGFP (93% identical to EGFP) in IP and Western blot?
   • For our product NB600-308, we have unfortunately not yet tested the product for cross reactivity with AcGFP. However, since the antibody is polyclonal and made to the full length AvGFP sequence, there is a good chance for cross reactivity.
2. Hello, my lab purchased a GFP antibody from Novus in the past and I am now in need of an antibody to detect YFP and CFP as well. The GFP antibody we order has a part number of NB600-308. Would it possibly recognize YPF and CFP as well or will I need to purchase additional antibodies?
   • NB600-308 is polyclonal to full length GFP, hence it will recognize most of its mutants. It will react with YFP and CFP.

Bioinformatics

• Uniprot:
  • P42212()