GFP Antibody

Western Blot: GFP Antibody [NB600-308] - Wild type GFP (0.1 ug) was used to spike HeLa whole cell lysate. Load: 30 ug per lane. Primary antibody: GFP antibody at 1:1000 for overnight at 4C. Secondary antibody: IR Dye800 Goat-anti-Rabbit IgG [H&L] MX10 at 1:10,000 for 45 min at RT. Block: 5% BLOTTO in PBS overnight at 4C. Predicted/Observed size: 27 kDa for epitope tag GFP. Other band(s): none.

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB600-308] - Analysis of GFP in transgenic mouse pancreas in OCT. The blue region in the center is an islet which does not express GFP and is negative. Image from verified customer review.

Western Blot: GFP Antibody [NB600-308] - analysis of GFP tagged PKA subunits over-expressed in HEK293T cells using GFP antibody. Image from verified customer review.

Immunohistochemistry: GFP Antibody [NB600-308] - Analysis of GFP in mouse retinal cells. Image courtesy of anonymous customer review.

Immunohistochemistry-Paraffin: GFP Antibody [NB600-308] - Staining of GFP+ hepatocytes (brown) transplanted into the liver of a recipient animal using anti-GFP antibody. Cyclin D1 double staining in blue. Image from verified customer review.

Immunohistochemistry-Frozen: GFP Antibody [NB600-308] - Adult mouse brain tissue. This image was submitted via customer review.

Immunoprecipitation: GFP Antibody [NB600-308] - IP analysis of GFP in S2 Cells (Drosophila). Image from verified customer review.

Immunoprecipitation: GFP Antibody [NB600-308] - Immunoprecipiation experiments showing that GFP-MISP binds endogenous IQGAP1 (upper panel) and MISP binds to IQGAP1 endogenously using specific antibodies (lower panel) in mitotically arrested (taxol and nocodazole, respectively) HeLa cell lysates. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24682-8), licensed under a CC-BY licence.

• Reactivity: All-NA
• Applications: WB, ELISA, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl, IHC-WhMt, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.99 mg/ml

GFP Antibody Summary

• Immunogen: This GFP antibody was developed against a fusion protein corresponding to the full length GFP (246aa) derived from the jellyfish Aequorea victoria.
• Specificity: Anti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). Primate reactivity reported in scientific literature (PMID: 27723741).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:5000
  • Electron Microscopy 1:10-1:500
  • ELISA 1:20000-1:120000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:5000
  • Immunohistochemistry 1:200-1:3000
  • Immunohistochemistry-Frozen 1:50-1:250
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
  • Knockdown Validated
• Application Notes: Use in Knockdown Validated reported in scientific literature (PMID: 32905777).
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

No reaction was observed against Human, Mouse serum proteins. Suitable for detecting fusion proteins containing the GFP sequence expressed in Human, Mouse, Rat, C. elegans, Drosophila and in vitro transcription/translation systems and transgenic animals. GFP Transgenic Rat reactivity reported in scientific literature (PMID:25724725). Mouse reactivity reported in multiple pieces of scientific literature.Transgenic C. elegans reactivity reported in scientific literature (PMID: 27110099). Known cross reactivity with wt and all variants such as rGFP, eGFP, S65T-GFP, RS-GFP, YFP and EGFP. Use in Mouse reported in secitific publication PMID: 32765228 Plant reactivity reported in scientific literature (PMID:32896843)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 0.99 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GFP Antibody

• anti-gfp (green fluorescent protein) pab
• eGFP
• Enhanced Green Fluorescent Protein
• GFP immunofluorescence
• GFP immunoprecipitation
• GFP mouse
• GFP staining
• GFP western blot
• GFP
• GFPuv
• green fluorescent protein (gfp)
• Green Fluorescent Protein

Background

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T.,... Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S.,... Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB600-308)(192)

Publications using NB600-308

• Tang C, Han J, Dalvi S et al. A human model of Batten disease shows role of CLN3 in phagocytosis at the photoreceptor-RPE interface Communications biology Feb 5 2021 [PMID: 33547385] (WB, Human, Mouse)
• González-Prieto R, Eifler-Olivi K, Claessens LA, et al. Global non-covalent SUMO interaction networks reveal SUMO-dependent stabilization of the non-homologous end joining complex Cell reports Jan 26 2021 [PMID: 33503430]
• Bland KM, Aharon A, Widener EL, et al. FMRP regulates the subcellular distribution of cortical dendritic spine density in a non-cell-autonomous manner Neurobiology of disease Jan 6 2021 [PMID: 33421563]
• Vergarajauregui S, Becker R, Steffen U et al. AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9 eLife Dec 9 2020 [PMID: 33295871] (Non-species specific)
• Wolter JM, Mao H, Fragola G et al. Cas9 gene therapy for Angelman syndrome traps Ube3a-ATS long non-coding RNA Nature Oct 21 2020 [PMID: 33087932]
• Sade N, Weng F, Tajima H et al. A Cytoplasmic Receptor-like Kinase Contributes to Salinity Tolerance Plants (Basel) Oct 17 2020 [PMID: 33080797]
• Im JY, Kim DM, Park H et al. VGLL1 phosphorylation and activation promotes gastric cancer malignancy via TGF-B/ERK/RSK2 signaling Biochim Biophys Acta Mol Cell Res Oct 16 2020 [PMID: 33069758] (WB)
• Donato C, Kunz L, Castro-Giner F et al. Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells Cell reports Sep 8 2020 [PMID: 32905777] (WB, KD, ICC/IF)
• Thagun C, Motoda Y, Kigawa T et al. Simultaneous introduction of multiple biomacromolecules into plant cells using a cell-penetrating peptide nanocarrier Nanoscale Sep 28 2020 [PMID: 32896843] (WB, Plant)
  
  Details:
  Nicotiana benthamiana
• Kim H, Berens N, Ochandarena N, Philpot B Region and Cell Type Distribution of TCF4 in the Postnatal Mouse Brain Front. Neuroanat. Jul 17 2020 [PMID: 32765228] (ICC/IF, Mouse)
• Saha C, Mohanraju P, Stubbs A et al. Guide-free Cas9 from pathogenic Campylobacter jejuni bacteria causes severe damage to DNA Sci Adv Jun 1 2020 [PMID: 32596446] (WB)
• Cuijpers SAG, Willemstein E, Ruppert JG et al. Chromokinesin KIF4A teams up with Stathmin 1 to regulate abscission in a SUMO-dependent manner J. Cell. Sci. Jun 26 2020 [PMID: 32591481] (WB)
• Frantz MG, Crouse EC, Sokhadze G et al. Layer 4 Gates Plasticity in Visual Cortex Independent of a Canonical Microcircuit Curr. Biol. Jun 18 2020 [PMID: 32589913]
• Shin E Y, Park J H et al. Integrin-mediated adhesions in regulation of cellular senescence. Sci Adv Jan 5 2020 [PMID: 32494696] (IHC, WB)
• Min H, Youn E, Shim YH Maternal Caffeine Intake Disrupts Eggshell Integrity and Retards Larval Development by Reducing Yolk Production in a Caenorhabditis elegans Model Nutrients May 7 2020 [PMID: 32392893] (WB, C. elegans)
• Chen Z, Wang ZH, Zhang G et al. Mitochondrial DNA segregation and replication restrict the transmission of detrimental mutation J. Cell Biol. Jul 6 2020 [PMID: 32375181] (ICC/IF)
• Taniguchi T, Zanetti-Yabur A, Wang P et al. Interindividual Diversity in Expression of Organic Anion Uptake Transporters in Normal and Cirrhotic Human Liver Hepatol Commun May 5 2020 [PMID: 32363323] (WB)
• Monette A, Niu M, Chen L et al. Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking Cell Rep Apr 23 2020 [PMID: 32320662] (WB)
• Steinberg EE, Gore F, Heifets BD et al. Amygdala-Midbrain Connections Modulate Appetitive and Aversive Learning Neuron Apr 16 2020 [PMID: 32294466]
• Kashiwagi H, Ishimoto H, Izumi SI et al. Human PZP and common marmoset A2ML1 as pregnancy related proteins Sci Rep Mar 22 2020 [PMID: 32198464] (IHC)
• Hu X, Deng Q, Ma L et al. Meningeal lymphatic vessels regulate brain tumor drainage and immunity Cell Res Feb 26 2020 [PMID: 32094452] (IHC-Fr)
• Tsukamoto T, Gearhart M D et al. Insights into the Involvement of Spliceosomal Mutations in Myelodysplastic Disorders from Analysis of SACY-1/DDX41 in Caenorhabditis elegans. Genetics Jan 4 2020 [PMID: 32060018] (WB)
• Corbet C, Bastien E, Santiago de Jesus JP et al. TGF beta 2-induced formation of lipid droplets supports acidosis-driven EMT and the metastatic spreading of cancer cells Nat Commun Jan 23 2020 [PMID: 31974393] (IHC, Mouse)
• Dubey R, Stivala CE, Nguyen HQ et al. Lipid droplets can promote drug accumulation and activation Nat. Chem. Biol. Feb 1 2020 [PMID: 31932720] (WB)
• Thagun C, Chuah JA, Numata K. Targeted Gene Delivery into Various Plastids Mediated by Clustered Cell-Penetrating and Chloroplast-Targeting Peptides Adv Sci (Weinh) Dec 14 2019 [PMID: 31832328] (IP, WB)
• Kim B K, Cheong J H et al. PI3K/AKT/beta-Catenin Signaling Regulates Vestigial-Like 1 Which Predicts Poor Prognosis and Enhances Malignant Phenotype in Gastric Cancer. Cancers (Basel) Mar 12 2019 [PMID: 31816819] (WB)
• Kunz L, Schroeder T A 3D Tissue-wide Digital Imaging Pipeline for Quantitation of Secreted Molecules Shows Absence of CXCL12 Gradients in Bone Marrow Cell Stem Cell Dec 5 2019 [PMID: 31809740] (ICC/IF, Mouse)
• Wang Z H, Liu Y et al. Electron transport chain biogenesis activated by a JNK-insulin-Myc relay primes mitochondrial inheritance in Drosophila. Elife Oct 15 2019 [PMID: 31612862] (IHC)
• Mercer Lindsay N, Knutsen PM, Lozada AF et al. Orofacial Movements Involve Parallel Corticobulbar Projections from Motor Cortex to Trigeminal Premotor Nuclei Neuron 2019 Sep 20 [PMID: 31587918]
• Gewin L, Summers ME, Harral JW et al. Inactivation of Tsc2 in Abcg2 Lineage Derived Cells Drives the Appearance of Polycystic Lesions and Fibrosis in the Adult Kidney Am. J. Physiol. Renal Physiol. Aug 28 2019 [PMID: 31461347]
• Guo B, Itami J et al. Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides. PLoS One Jul 31 2019 [PMID: 31361745] (WB)
• Zusso M, Lunardi V, Franceschini D et al. Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway J Neuroinflammation Jul 18 2019 [PMID: 31319868] (WB, Mouse)
• Shi C, Pan FC, Kim JN et al. Differential cell susceptibilities to KrasG12D in the setting of obstructive chronic pancreatitis Cell Mol Gastroenterol Hepatol Jul 13 2019 [PMID: 31310834] (IHC, Mouse)
• English JG, Olsen RHJ, Lansu K et al. VEGAS as a Platform for Facile Directed Evolution in Mammalian Cells Cell Jul 25 2019 [PMID: 31280962]
• Lee M, Hwang Y S et al. Developmentally regulated GTP-binding protein 1 modulates ciliogenesis via an interaction with Dishevelled. J Cell Biol Mar 7 2019 [PMID: 31270137] (IHC, IP)
• Nilangekar K, Murmu N, Sahu G et al. Generation and Characterization of Germline-Specific Autophagy and Mitochondrial Reactive Oxygen Species Reporters in Drosophila Front Cell Dev Biol Apr 20 2019 [PMID: 31001531] (WB)
• Cruz-Ortega JS, Boucard AA. Actin cytoskeleton remodeling defines a distinct cellular function for adhesion G protein-coupled receptors ADGRL/latrophilins 1, 2 and 3 Biol Open Mar 31 2019 [PMID: 30926595] (WB)
• Sandhu R, Wei D, Sharma M et al. An N-terminal Flag-tag impairs TPP1 regulation of telomerase function Biochem Biophys Res Commun Mar 20 2019 [PMID: 30885434] (WB)
• Li A, Cho JH, Reid B et al. Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks Nat Commun Dec 19 2018 [PMID: 30560870] (IHC)
• Di Scala C, Tessier M, Sapet C et al. A new polymer-based approach for in vivo transfection in postnatal brain. J Neurosci Methods 2019 Jan 1 [PMID: 30408559]
• Li Q, Saito TT, Martinez-Garcia M et al. The tumor suppressor BRCA1-BARD1 complex localizes to the synaptonemal complex and regulates recombination under meiotic dysfunction in Caenorhabditis elegans PLoS Genet Nov 2 2018 [PMID: 30383767] (WB)
• Zhao S, Fortier TM, Baehrecke EH. Autophagy Promotes Tumor-like Stem Cell Niche Occupancy Curr Biol Oct 2 2018 [PMID: 30270184] (ICC/IF)
• Cain NE, Jahed Z, Schoenhofen A et al. Conserved SUN-KASH Interfaces Mediate LINC Complex-Dependent Nuclear Movement and Positioning Curr Biol Sep 25 2018 [PMID: 30245107] (ICC/IF)
• Spike CA, Huelgas-Morales G, Tsukamoto T et al. Multiple Mechanisms Inactivate the LIN-41 RNA-Binding Protein To Ensure a Robust Oocyte-to-Embryo Transition in Caenorhabditis elegans Genetics Sep 13 2018 [PMID: 30206186] (WB)
• Wang L, Chen SR, Ma H et al. Regulating nociceptive transmission by VGluT2-expressing spinal dorsal horn neurons. J. Neurochem. Sep 11 2018 [PMID: 30203849] (IHC, Mouse)
• Lee HM, Clark EP, Kuijer MB et al. Characterization and structure-activity relationships of indenoisoquinoline-derived topoisomerase I inhibitors in unsilencing the dormant Ube3a gene associated with Angelman syndrome Mol Autism Aug 17 2018 [PMID: 30140420] (ICC/IF, Mouse)
• Yao H, Price TT, Cantelli G et al. Leukaemia hijacks a neural mechanism to invade the central nervous system Nature Jul 20 2018 [PMID: 30022166] (IHC)
• Nakano Y, Kelly MC, Rehman AU et al. Defects in the Alternative Splicing-Dependent Regulation of REST Cause Deafness Cell Jul 3 2018 [PMID: 29961578] (ICC/IF)
• Beer-Hammer S, Lee SC, Mauriac SA et al. Gai Proteins are Indispensable for Hearing. Cell. Physiol. Biochem. Jun 21 2018 [PMID: 29940568] (IHC)
• Stephany CE, Ma X, Dorton HM et al. Distinct Circuits for Recovery of Eye Dominance and Acuity in Murine Amblyopia Curr Biol Jun 12 2018 [PMID: 29887305]
• Bell P, Yu H, Kuntz L et al. Preparation of Nonhuman Primate Eyes for Histological Evaluation After Retinal Gene Transfer Hum Gene Ther Methods Jun 1 2018 [PMID: 29848071] (IHC)
• Du P, Pirouz M, Choi J et al. An Intermediate Pluripotent State Controlled by MicroRNAs Is Required for the Naive-to-Primed Stem Cell Transition Cell Stem Cell May 16 2018 [PMID: 29804889] (IHC)
• Herrera LA, Starr DA The E3 Ubiquitin Ligase MIB-1 Is Necessary To Form the Nuclear Halo in Caenorhabditis elegans Sperm G3 (Bethesda). 2018 May 17 [PMID: 29776970] (IHC, C. elegans)
• Shi B, Xue M, Wang Y et al. An improved method for increasing the efficiency of gene transfection and transduction Int J Physiol Pathophysiol Pharmacol May 15 2018 [PMID: 29755642] (ICC/IF)
• Furuta T, Joo HJ, Trimmer KA et al. GSK-3 promotes S-phase entry and progression in C. elegans germline stem cells to maintain tissue output Development Apr 27 2018 [PMID: 29695611]
• Vodicska B, Cerikan B, Schiebel E et al. MISP regulates the IQGAP1/Cdc42 complex to collectively orchestrate spindle orientation and mitotic progression Sci Rep Apr 22 2018 [PMID: 29679050] (ICC/IF, WB)
• O'Neill S, Mathis M, Kovacic L et al. Quantitative interaction analysis permits molecular insights into functional NOX4 NADPH oxidase heterodimer assembly J Biol Chem Apr 21 2018 [PMID: 29674345] (IP)
• Lebeau P, Platko K, Al-Hashimi AA et al. Loss-of-function PCSK9 mutants evade the unfolded protein response sensor GRP78 and fail to induce endoplasmic reticulum stress when retained. J Biol Chem 2018 May 11 [PMID: 29593095] (Mouse)
• Finn JD, Smith AR, Patel MC et al. A Single Administration of CRISPR/Cas9 Lipid Nanoparticles Achieves Robust and Persistent In Vivo Genome Editing Cell Rep Mar 1 2018 [PMID: 29490262]
• Dell'Aringa M, Reinhardt RL. Notch signaling represents an important checkpoint between follicular T-helper and canonical T-helper 2 cell fate Mucosal Immunol Feb 23 2018 [PMID: 29467447] (ICC/IF)
• Wang A, Conicella AE, Schmidt HB, Martin EW. A single N-terminal phosphomimic disrupts TDP-43 polymerization, phase separation, and RNA splicing. EMBO J. 2018 Feb 09 [PMID: 29438978] (WB)
• Pusapati GV, Kong JH, Patel BB et al. G protein-coupled receptors control the sensitivity of cells to the morphogen Sonic Hedgehog Sci Signal Feb 14 2018 [PMID: 29438014]
• Linden AG, Li S, Choi HY et al. Interplay between ChREBP and SREBP-1c coordinates postprandial glycolysis and lipogenesis in livers of mice. J. Lipid Res. Mar 1 2018 [PMID: 29335275] (WB, Mouse)
• Buzgariu W, Wenger Y, Tcaciuc N et al. Impact of cycling cells and cell cycle regulation on Hydra regeneration Dev Biol Jan 3 2018 [PMID: 29291976] (ICC/IF)
• Brumbaugh J, Di Stefano B, Wang X et al. Nudt21 Controls Cell Fate by Connecting Alternative Polyadenylation to Chromatin Signaling Cell Dec 19 2017 [PMID: 29249356]
• Herring CA, Banerjee A, McKinley ET et al. Unsupervised Trajectory Analysis of Single-Cell RNA-Seq and Imaging Data Reveals Alternative Tuft Cell Origins in the Gut Cell Syst. 2017 Nov 07 [PMID: 29153838] (Mouse)
• Sade N, Umnajkitikorn K, Rubio Wilhelmi MDM et al. Delaying chloroplast turnover increases water-deficit stress tolerance through the enhancement of nitrogen assimilation in rice J Exp Bot. 2018 Feb 05 [PMID: 28992306] (WB, Plant)
  
  Details:
  WB was performed using Oryza sativa lysates.
• Grimaldo L, Sandoval A, Garza-Lopez E et al. Involvement of Parkin in the ubiquitin proteasome system-mediated degradation of N-type voltage-gated Ca2+ channels PLoS One Sep 29 2017 [PMID: 28957379] (IP, WB)
• Laszczyk AM, Fox-Quick S, Vo HT et al. Klotho regulates postnatal neurogenesis and protects against age-related spatial memory loss Neurobiol. Aging 2017 Jul 29 [PMID: 28837861] (IHC-FrFl, Mouse)
• Ogasawara T, Okano S, Ichimura H et al. Impact of extracellular matrix on engraftment and maturation of pluripotent stem cell-derived cardiomyocytes in a rat myocardial infarct model. Sci Rep 2017 Aug 17 [PMID: 28819182] (IHC)
• Bellavance MA, Takatoh J, Lu J et al. Parallel Inhibitory and Excitatory Trigemino-Facial Feedback Circuitry for Reflexive Vibrissa Movement Neuron 2017 Jul 13 [PMID: 28735746]
• Cardoso MA, Fontenele M, Lim B et al. A novel function for the IkappaB inhibitor Cactus in promoting Dorsal nuclear localization and activity in the Drosophila embryo Development Jul 15 2017 [PMID: 28705899] (ICC/IF)
• Yoon S, Kawasaki I, Shim YH. cyb-1, a C. elegans B-type cyclin, maintains proper position and number of centrosomes during spermatogenesis J. Cell. Sci. 2017 Jul 13 [PMID: 28705837] (ICC/IF, C. elegans)
• Stauffer BB, Cui G, Cottrill KA et al. Bacterial Sphingomyelinase is a State-Dependent Inhibitor of the Cystic Fibrosis Transmembrane conductance Regulator (CFTR) Sci Rep Jun 9 2017 [PMID: 28592822] (ELISA)
• Patra C, Kontarakis Z, Kaur H et al. The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies Sci Rep Jun 5 2017 [PMID: 28578380] (IHC)
• Witzel HR, Cheedipudi S, Gao R et al. Isl2b regulates anterior second heart field development in zebrafish Sci Rep Jan 21 2017 [PMID: 28106108] (ICC/IF)
• Ono Y, Perez-Gutierrez A et al. Regeneration and Cell Recruitment in an Improved Heterotopic Auxiliary Partial Liver Transplantation Model in the Rat. Transplantation Jan 1 2017 [PMID: 28009756] (ICC/IF)
• Dufeys C Exploring the role of fibroblastic AMPKalpha1 in ventricular remodelling after myocardial infarction: implication of connexin 43 and miR-125b Thesis 2019
• Goetsch PD, Strome S DREAM Interrupted: Severing MuvB from DREAM's pocket protein impairs gene repression but not DREAM assembly on chromatin BioRxiv 2019 Jun 13
• Yu D, Maroteaux M, Song Y et al. The transcription factor Shox2 shapes thalamocortical neuron firing properties by regulation of key ion channels bioRxiv (IHC, WB)
• Yovchev MI, Oertel M. Fetal Liver Stem/Progenitor Cell Transplantation: A Model to Study Tissue Mass Replacement and Cell-Based Therapies. Methods Mol. Biol. Nov 10 2016 [PMID: 27830548]
• Maniates KA, Olson BS, Abbott AL Sperm fate is promoted by the mir-44 microRNA family in the Caenorhabditis elegans hermaphrodite germline Genetics Jan 1 2020 (IHC)
• Tsukamoto T, Gearhart MD, Spike CA et al. LIN-41 and OMA Ribonucleoprotein Complexes Mediate a Translational Repression-to-Activation Switch Controlling Oocyte Meiotic Maturation and the Oocyte-to-Embryo Transition in Caenorhabditis elegans. Genetics. 2017 Jun 01 [PMID: 28576864] (WB, C. elegans)
• Knutson AK, Egelhofer T, Rechtsteiner A, Strome S. Germ Granules Prevent Accumulation of Somatic Transcripts in the Adult Caenorhabditis elegans Germline. Genetics. Mar 3 2017 [PMID: 28258184] (ICC/IF, C. elegans)
• Davidson R, Pontasch JA, Wu JQ. Sbg1 Is a Novel Regulator for the Localization of the beta-Glucan Synthase Bgs1 in Fission Yeast PLoS One Nov 30 2016 [PMID: 27898700] (IP, WB)
• Lee JJ, Rothenberg ME, Seeley ES et al. Control of inflammation by stromal Hedgehog pathway activation restrains colitis. Proc. Natl. Acad. Sci. U.S.A. Nov 22 2016 [PMID: 27815529] (Mouse)
• Zhang W, Lanzoni G, Hani H et al. Patch Grafting of Cells into Solid Organs Such as Liver and Pancreas SSRN Journal Jun 19 2020
• Morales L, Castro-Robles B, AbellAn A et al. A novel telencephalon-opto-hypothalamic morphogenetic domain produces most of the glutamatergic neurons of the medial extended amygdala bioRxiv Jul 18 2020 (IHC, Mouse)
• Moon H, Zhu J, Donahue LR et al. Electron transport chain biogenesis activated by a JNK-insulin-Myc relay primes mitochondrial inheritance in Drosophila bioRxiv May 18 2019 (IHC, Drosophila)
• Cherian JR Characterizing the Mechanisms of Ectopic Expression of Dream Complex Target Genes and the HTA Phenotype in C. Elegans Thesis Jan 1 2020 (C. elegans)
• Greenstein D, Tsukamoto T, Gearhart MD, Kim S Insights into the involvement of spliceosomal mutations in myelodysplastic disorders from an analysis of SACY-1/DDX41 in Caenorhabditis elegans bioRxiv Dec 23 2019 (WB, C. elegans)
• Chowdhury, A Tamalin/GRAS-1 Connects Glutamate Receptor Activity to the Insulin/IGF Signaling Cascade to Regulate Neuroprotection in a Nematode Model of Excitotoxicity Thesis Jan 1 2020 (IP, C. elegans)
• Autry AE, Wu Z, Kohl J, Bambah-Mukku D Perifornical Area Urocortin-3 Neurons Promote Infant-directed Neglect and Aggression bioRxiv Jul 9 2019 (IHC, Mouse)
• Bao K, Carr T, Wu J et al. BATF Modulates the Th2 Locus Control Region and Regulates CD4+ T Cell Fate during Antihelminth Immunity J Immunol Nov 1 2016 [PMID: 27798167] (IHC)
• Shiba Y, Gomibuchi T, Seto T et al. Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts Nature Oct 11 2016 [PMID: 27723741] (IHC-P)
• Ahn JH, Rechsteiner A, Strome S, Kelly WG. A Conserved Nuclear Cyclophilin Is Required for Both RNA Polymerase II Elongation and Co-transcriptional Splicing in Caenorhabditis elegans. PLoS Genet. Aug 1 2016 [PMID: 27541139] (ICC/IF, IP)
• Liu Y, Lee IJ, Sun M et al. Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis Mol Biol Cell Jul 8 2016 [PMID: 27385337] (IP)
• Barrionuevo FJ, Hurtado A, Kim GJ et al. Sox9 and Sox8 protect the adult testis from male-to-female genetic reprogramming and complete degeneration. Elife Jun 21 2016 [PMID: 27328324] (IHC, Mouse)
• Zeng Y, Guo W, Xu G et al. Xyloketal-derived small molecules show protective effect by decreasing mutant Huntingtin protein aggregates in Caenorhabditis elegans model of Huntington's disease. Drug Des Devel Ther. 2016 Apr 25 [PMID: 27110099] (WB, C. elegans)
• Myeku N, Clelland CL, Emrani S et al. Tau-driven 26S proteasome impairment and cognitive dysfunction can be prevented early in disease by activating cAMP-PKA signaling. Nat. Med. 2016 Jan 01 [PMID: 26692334] (WB)
• Sorrentino NC, Maffia V, Strollo S et al. A Comprehensive Map of CNS Transduction by Eight Recombinant Adeno-associated Virus Serotypes Upon Cerebrospinal Fluid Administration in Pigs. Mol. Ther. 2015 Dec 07 [PMID: 26639405] (WB, IHC-FrFl, IHC-Fr)
• Gross S, Balderes D, Liu J et al. Nkx2.2 is expressed in a subset of enteroendocrine cells with expanded lineage potential. Am J Physiol Gastrointest Liver Physiol 2015 Dec 15 [PMID: 26492922]
• Rueckemann JW, DiMauro AJ, Rangel LM et al. Transient optogenetic inactivation of the medial entorhinal cortex biases the active population of hippocampal neurons Hippocampus Aug 25 2015 [PMID: 26299904] (IHC-Fr)
• Akhtar Ali M, Younis S, Wallerman O et al. Transcriptional modulator ZBED6 affects cell cycle and growth of human colorectal cancer cells Proc Natl Acad Sci U S A Jun 10 2015 [PMID: 26056301] (WB)
• Gabrusiewicz K, Hossain MB, Cortes-Santiago N et al. Macrophage Ablation Reduces M2-Like Populations and Jeopardizes Tumor Growth in a MAFIA-Based Glioma Model Neoplasia 2015 Apr 01 [PMID: 25925380] (ICC/IF, IHC-P, Mouse)
• Gautam M, Fujita D, Kimura K et al. Transplantation of adipose tissue-derived stem cells improves cardiac contractile function and electrical stability in a rat myocardial infarction model J Mol Cell Cardiol Mar 1 2015 [PMID: 25724725] (IHC-P)
• Doi K, Ogata F, Eto H et al. Differential contributions of graft- and host-derived cells in tissue regeneration/remodeling after fat grafting Plast. Reconstr. Surg. 2015 Feb 20 [PMID: 25724061] (IHC-P)
• Matthews DW, Deschenes M, Furuta T et al. Feedback in the brainstem: An excitatory disynaptic pathway for control of whisking. J. Comp. Neurol. 2014 Dec 12 [PMID: 25503925] (IHC, Mouse)
• Li M, Bian C, Yu X. Poly(ADP-ribosyl)ation is recognized by ECT2 during mitosis. Cell Cycle 2014 [PMID: 25486481] (WB)
• Davidson R, Laporte D, Wu JQ. Regulation of Rho GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis. Mol. Biol. Cell. 2014 Dec 03 [PMID: 25473118] (IP)
  
  Details:
  GFP antibody used in IP for pulling down the YFP-tagged protein expressed at its native level from fission yeast cell extracts (Figure 3E)
• Meng L, Ward AJ, Chun S et al. Towards a therapy for Angelman syndrome by targeting a long non-coding RNA. Nature. 2014 Dec 01 [PMID: 25470045] (WB)
• Liu D, Yovchev Mi, Zhang J et al. Identification and Characterization of Mesenchymal-Epithelial Progenitor-Like Cells in normal and Injured Rat Liver. Am. J. Pathol. 2014 Nov 06 [PMID: 25447047]
• Yap MY, Lo YL, Talbot K et al. Oxidative stress reduces levels of dysbindin-1A via its PEST domain Neurochem Int Dec 3 2014 [PMID: 25445987] (WB)
• Nelson C, Ambros V, Baehrecke Eh. miR-14 Regulates Autophagy during Developmental Cell Death by Targeting ip3-kinase 2. Mol. Cell. 2014 Oct 08 [PMID: 25306920] (WB)
• Bratton B, Wright Cw. NF-kB-inducing kinase (nIK) contains an amino terminal inhibitor of apoptosis (IAP) binding motif (IBM) that potentiates nIK degradation by c-IAP1* J. Biol. Chem. et al. 2014 Sep 22 [PMID: 25246529] (WB)
• Cain NE, Tapley EC, McDonald KL et al. The SUN protein UNC-84 is required only in force-bearing cells to maintain nuclear envelope architecture. J. Cell Biol. 2014 Jul 21 [PMID: 25023515] (IHC-WhMt)
  
  Details:
  GFP antibody used for immunofluorescence staining of C elegans embryos - antibody diluted 1:200 in UNC-83 antiserum for simultaneous staining of KASH and SUN chimeric proteins.
• Shigunov Patricia, Sotelo-Silveira Jose, Stimamiglio Marco Augusto et al. Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules. BMC Mol Biol. 2014 Jul 03 [PMID: 24993635] (IP)
• Wang N, Lo Presti L, Zhu YH et al. The novel proteins Rng8 and Rng9 regulate the myosin-V Myo51 during fission yeast cytokinesis. J. Cell Biol. 2014 May 12 [PMID: 24798735] (IP)
• Buzgariu W, Crescenzi M, Galliot B. Robust G2 pausing of adult stem cells in Hydra. Differentiation 2014 Apr 21 [PMID: 24703763] (ICC/IF, IHC-WhMt)
• Walter TJ, Vanderpool C, Cast AE, Huppert SS. Intrahepatic Bile Duct Regeneration in Mice Does Not Require Hnf6 or Notch Signaling through Rbpj. Am. J. Pathol. 3/13/2014 [PMID: 24631193] (IHC-P)
• Klos KS, Warmka JK, Drachenberg DM et al. Building Bridges toward Invasion: Tumor Promoter Treatment Induces a Novel Protein Kinase C-Dependent Phenotype in MCF10A Mammary Cell Acini. PLoS ONE 3/6/2014 [PMID: 24599099] (ICC/IF)
• Montgomery Scott R, Nargizyan Taya, Meliton Vicente et al. A novel osteogenic oxysterol compound for therapeutic development to promote bone growth: activation of hedgehog signaling and osteogenesis through smoothened binding. J Bone Miner Res. 2014 Aug 01 [PMID: 24591126]
• Hagiwara M, Kokubu E, Sugiura S et al. Vinculin and Rab5 Complex Is Requited for Uptake of Staphyrococcus aureus and Interleukin-6 Expression. PLoS ONE 2014 Jan 27 [PMID: 24466349] (WB, IP)
• Tian G, Ropelewski P, Nemet I et al. An unconventional secretory pathway mediates the Cilia targeting of peripherin/rds. J. Neurosci. 2014 Jan 15 [PMID: 24431457] (WB)
• Nachtergaele S, Whalen DM, Mydock LK et al. Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling. Elife. 2013 Oct 29 [PMID: 24171105] (WB)
• Oldenbroek M, Robertson SM, Guven-Ozkan T et al. Regulation of maternal Wnt mRNA translation in C. elegans embryos. Development. 2013 Nov [PMID: 24131629] (IP)
• Hullinger R, Ugalde J, Puron-Sierra L et al. The MRI contrast agent gadoteridol enhances distribution of rAAV1 in the rat hippocampus. Gene Ther. 2013 Sep 19 [PMID: 24048419] (IHC-Fr)
• Zhu YH, Ye Y, Wu Z et al. Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis Mol Biol Cell Aug 23 2013 [PMID: 23966468] (IP)
• Park BH, Kook S, Lee S et al. An isoform of C/EBPbeta, LIP, regulates expression of the chemokine receptor CXCR4 and modulates breast cancer cell migration. J Biol Chem. 2013 Oct 4 [PMID: 23966000] (IHC-P)
• Chang TK, Shravage BV, Hayes SD et al. Uba1 functions in Atg7- and Atg3-independent autophagy Nat Cell Biol Jul 23 2013 [PMID: 23873149] (EM)
• Huang WC, Yang CC, Chen IH et al. Treatment of Glucocorticoids Inhibited Early Immune Responses and Impaired Cardiac Repair in Adult Zebrafish PLoS One Jun 28 2013 [PMID: 23805247] (IHC-Fr, ICC/IF)
• Wang G, Li Y, Wang XY et al. Slit/Robo1 signaling regulates neural tube development by balancing neuroepithelial cell proliferation and differentiation Exp Cell Res Feb 27 2013 [PMID: 23438940] (IHC, ICC/IF)
• Yang JR, Hsu CW, Liao SC et al. Transplantation of embryonic stem cells improves the regeneration of periodontal furcation defects in a porcine model J Clin Periodontol Feb 26 2013 [PMID: 23431981] (IHC-P)
• Shravage BV, Hill JH, Powers CM et al. Atg6 is required for multiple vesicle trafficking pathways and hematopoiesis in Drosophila Development Feb 15 2013 [PMID: 23406899] (IHC-P)
• Vergara MN, Gutierrez C, O'Brien DR et al. Ex vivo electroporation of retinal cells: a novel, high efficiency method for functional studies in primary retinal cultures Exp Eye Res Feb 2 2013 [PMID: 23370269] (ICC/IF)
• Shinojima N, Hossain A, Takezaki T et al. TGF-beta mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells Cancer Res Feb 1 2013 [PMID: 23365134] (ICC/IF)
• Wu Y, Wang K, Karapetyan A et al. Connective Tissue Fibroblast Properties Are Position-Dependent during Mouse Digit Tip Regeneration. PLoS One 2013 [PMID: 23349966] (IHC, ICC/IF)
• Hauser J, Verma-Gaur J, Grundstrom T. Broad feedback inhibition of pre-B-cell receptor signaling components. Mol Immunol 2013 Jan 11 [PMID: 23318223] (Mouse)
• Cho JH, Kam JW, Cloutier JF. Slits and Robo-2 regulate the coalescence of subsets of olfactory sensory neuron axons within the ventral region of the olfactory bulb Dev Biol 2012 Sep 7 [PMID: 22981605] (IHC, ICC/IF, Mouse)
• Xia L, Yin S, Wang J. Inner ear gene transfection in neonatal mice using adeno-associated viral vector: a comparison of two approaches PLoS One 2012 [PMID: 22912830] (IHC-Fr, Mouse)
• Georg I, Barrionuevo F, Wiech T, Scherer G. Sox9 and Sox8 Are Required for Basal Lamina Integrity of Testis Cords and for Suppression of FOXL2 During Embryonic Testis Development in Mice Biol Reprod 2012 Oct 25 [PMID: 22837482]
• Fabbri M, Paone A, Calore F et al. MicroRNAs bind to Toll-like receptors to induce prometastatic inflammatory response Proc Natl Acad Sci U S A Jul 4 2012 [PMID: 22753494] (WB)
• Lin CS, Lin FY, Ho LJ et al. PKCdelta signalling regulates SR-A and CD36 expression and foam cell formation Cardiovasc Res Jun 13 2012 [PMID: 22687273] (WB)
• Ahmed M, Xu J, Xu PX. EYA1 and SIX1 drive the neuronal developmental program in cooperation with the SWI/SNF chromatin-remodeling complex and SOX2 in the mammalian inner ear. Development;139(11):1965-77. 2012 Jun. [PMID: 22513373] (IHC, Mouse)
• Jin H, Zangar RC. High-throughput, multiplexed analysis of 3-nitrotyrosine in individual proteins. Curr Protoc Toxicol 2012 Feb [PMID: 22511115]
• Meng L, Person RE, Beaudet AL. Ube3a-ATS is an atypical RNA polymerase II transcript that represses the paternal expression of Ube3a. Hum Mol Genet. 2012 Apr 18. [PMID: 22493002] (ICC/IF, Mouse)
• Powell AE, Wang Y, Li Y et al. The Pan-ErbB Negative Regulator Lrig1 Is an Intestinal Stem Cell Marker that Functions as a Tumor Suppressor Cell 2012 Mar 30 [PMID: 22464327] (IHC-Fr, ICC/IF, Mouse)
• Lee IJ, Wu JQ. Characterization of Mid1 domains for targeting and scaffolding in fission yeast cytokinesis. J Cell Sci 2012 Jun 15 [PMID: 22427686] (WB)
• Ahmed M, Wong EY, Sun J et al. Eya1-six1 interaction is sufficient to induce hair cell fate in the cochlea by activating atoh1 expression in cooperation with sox2. Dev Cell;22(2):377-90. 2012 Feb 14. [PMID: 22340499]
• Rizzolio F, Lucchetti C, Caligiuri I et al. Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1. Cell Death Differ 2012 Jul [PMID: 22322860]
• Canton J, Ndjamen B, Hatsuzawa K, Kima PE. Disruption of the fusion of Leishmania parasitophorous vacuoles with ER vesicles results in the control of the infection. Cell Microbiol doi: 10.1111/j.1462-5822.2012.01767.x. 2012 Feb 6. [PMID: 22309219]
• Ye Y, Lee IJ, Runge KW et al. Roles of putative Rho-GEF Gef2 in division-site positioning and contractile-ring function in fission yeast cytokinesis Mol Biol Cell Feb 3 2012 [PMID: 22298427] (IP)
• Nachtergaele S, Mydock LK, Krishnan K et al. Oxysterols are allosteric activators of the oncoprotein Smoothened. Nat Chem Biol 2012 Feb [PMID: 22231273] (WB)
• Venters SJ, Cuenca PD, Hyer J et al. Retinal and anterior eye compartments derive from a common progenitor pool in the avian optic cup. Mol Vis 2011 [PMID: 22219630]
• Huang H-S, Allen JA, Mabb AM et al. Topoisomerase inhibitors unsilence the dormant allele of Ube3a in neurons. Nature. 2011 Dec 21. [PMID: 22190039]
• Liang H-E, Reinhardt RL, Bando JK et al. Divergent expression patterns of IL-4 and IL-13 define unique functions in allergic immunity. Nature Immunology. 2011 Dec 4. [PMID: 22138715]
• Fang L, Green SR, Baek JS et al. In vivo visualization and attenuation of oxidized lipid accumulation in hypercholesterolemic zebrafish. J Clin Invest. 2011 Nov 21. [PMID: 22105168]
• Pane A, Jiang P, Zhao DY et al. The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline. The EMBO Journal. 2011 Sep 27. [PMID: 21952049]
• Tian H, Biehs B, Warming S et al. A reserve stem cell population in small intestine renders Lgr5-positive cells dispensable. Nature. 2011 Sep 18. [PMID: 21927002]
• Ninagawa N, Murakami R, Isobe E et al. Mesenchymal stem cells originating from ES cells show high telomerase activity and therapeutic benefits. Differentiation. 2011 Aug 18. [PMID: 21855203]
• Schmidt FI, Bleck CK, Helenius A et al. Vaccinia extracellular virions enter cells by macropinocytosis and acid-activated membrane rupture. EMBO J 2011 Jul [PMID: 21792173]
• Damulewicz M, Pyza E. The clock input to the first optic neuropil of Drosophila melanogaster expressing neuronal circadian plasticity. PLoS One 2011 [PMID: 21760878]
• Safina A, Sotomayor P, Limoge M et al. TAK1-TAB2 signaling contributes to bone destruction by breast carcinoma cells. Mol Cancer Res. 2011 Jun 2. [PMID: 21700681]
• Wang H, Murphy R, Taaffe D et al. Efficient cochlear gene transfection in guinea-pigs with adeno-associated viral vectors by partial digestion of round window membrane. Gene Ther 2012 Mar [PMID: 21697953] (ICC/IF)
• Chen X, Nelson CD, Li X et al. PSD-95 is required to sustain the molecular organization of the postsynaptic density J Neurosci Apr 29 2011 [PMID: 21525273]
• Laporte D, Coffman VC, Lee IJ, Wu JQ. Assembly and architecture of precursor nodes during fission yeast cytokinesis. J Cell Biol 2011 Mar 21 [PMID: 21422229] (IP)
• Lauzier A, Charbonneau M, Harper K et al. Formation of invadopodia-like structures by synovial cells promotes cartilage breakdown in collagen-induced arthritis: involvement of the protein tyrosine kinase Src Arthritis Rheum 2011 Jun [PMID: 21311774] (IHC, Mouse)
• Sparks EE, Perrien DS, Huppert KA et al. Defects in hepatic Notch signaling result in disruption of the communicating intrahepatic bile duct network in mice. Dis Model Mech. 41405 [PMID: 21282722] (IHC-Fr, Mouse)
• Torres J, Funk HM, Zegers MM et al. The syntaxin 4 N terminus regulates its basolateral targeting by munc18c-dependent and -independent mechanisms. J Biol Chem 2011 Mar [PMID: 21278252]
• S Dindot, P Piccolo, NC Grove et al. Intrathecal injection of HDAd vectors results in long-term transgene expression in neuroependymal cells and neurons. Hum Gene Ther. 2010 Dec 22. [PMID: 21175294]
• Papadopoulos C, Arato K, Lilienthal E et al. Splice variants of the dual specificity tyrosine phosphorylation-regulated kinase 4 (DYRK4) differ in their subcellular localization and catalytic activity. J Biol Chem 2011 Feb [PMID: 21127067]
• Fridolfsson HN, Starr DA. Kinesin-1 and dynein at the nuclear envelope mediate the bidirectional migrations of nuclei. J Cell Biol 2010 Oct 4 [PMID: 20921138] (ICC/IF)
• Xue X, Jaulin F, Espenel C, Kreitzer G. PH-domain-dependent selective transport of p75 by kinesin-3 family motors in non-polarized MDCK cells J Cell Sci 2010 May 15 [PMID: 20427314] (IP)
• Jaramillo-Lambert A, Engebrecht J. A Single Unpaired and Transcriptionally Silenced X Chromosome Locally Precludes Checkpoint Signaling in the Caenorhabditis elegans Germ Line. Genetics. [PMID: 20008570]
• Fridolfsson HN, Ly N, Meyerzon M, Starr DA. UNC-83 coordinates kinesin-1 and dynein activities at the nuclear envelope during nuclear migration. Dev Biol 2010 Feb 15 [PMID: 20005871] (WB, ICC/IF)
• Steeg C, Adler G, Sparwasser T et al. Limited role of CD4+Foxp3+ regulatory T cells in the control of experimental cerebral malaria. J Immunol;183(11):7014-22. 2009 Dec 1. [PMID: 19890049]
• Meyerzon M, Fridolfsson HN, Ly N et al. UNC-83 is a nuclear-specific cargo adaptor for kinesin-1-mediated nuclear migration Development Jul 17 2009 [PMID: 19605495] (ICC/IF)
• Tokui K, Adachi H, Waza M et al. 17-DMAG ameliorates polyglutamine-mediated motor neuron degeneration through well-preserved proteasome function in an SBMA model mouse. Hum Mol Genet;18(5):898-910. 2009 [PMID: 19066230]
• Hoffman GE, Le WW, Sita LV. The importance of titrating antibodies for immunocytochemical methods. Curr Protoc Neurosci. 2008 Oct [PMID: 18972376]
• Warzecha CC, Jiang P, Amirikian K et al. Dermal-type macrophages expressing CD209/DC-SIGN show inherent resistance to dengue virus growth. PLoS Negl Trop Dis 2008 Oct [PMID: 18827881]
• Shafikhani SH, Mostov K, Engel J. Focal adhesion components are essential for mammalian cell cytokinesis Cell Cycle Sep 13 2008 [PMID: 18787414] (WB)
• Dindot, SV et al. The Angelman syndrome ubiquitin ligase localizes to the synapse nucleus, maternal deficiency results in abnormal dendritic spine morphology. Hum Mol Genet 17(1): 111-118. 2008 [PMID: 17940072]
• Shafikhani, HS, Engel, Joanne. Pseudomonas aeruginosa type III-secreted toxin ExoT inhibits host-cell division by targeting cytokinesis at multiple steps. PNAS 103:15605-15610. 2006 [PMID: 17030800]
• Reinhardt, R L, Hong, S, Kang, S-J et al. Visualization of IL-12/23p40 in vivo reveals immunostimulatory dendritic cell migrants that promote Th1 differentiation. J Immunol 177:1618-1627. 2006 [PMID: 16849470]
• Tretyakova I, Zolotukhin AS, Tan W et al. Nuclear export factor family protein participates in cytoplasmic mRNA trafficking. J Biol Chem 2005 Sep 9 [PMID: 16014633]
• Day, Y-J et al. A2a Adenosine receptors on bone marrow-derived cells protect liver from ischemia-reperfusion injury. J Immunol 174:5040-5046. 2005 [PMID: 15814735]
• Green CB, Zhao X, Hoyer LL. Use of green fluorescent protein and reverse transcription-PCR to monitor Candida albicans agglutinin-like sequence gene expression in a murine model of disseminated candidiasis. Infect Immun 2005 Mar [PMID: 15731087]
• Bowman AB, Yoo SY, Dantuma NP et al. Neuronal dysfunction in a polyglutamine disease model occurs in the absence of ubiquitin-proteasome system impairment and inversely correlates with the degree of nuclear inclusion formation. Hum Mol Genet;14(5):679-91. 2005 Mar 1. [PMID: 15661755]
• Merkle, FT et al. Radial glia give rise to adult neural stem cells in the subventricular zone. PNAS 101:17528-17532. 2004 [PMID: 15574494]
• Kanazawa, Y, Verma, IM. Little evidence of bone moarrow-derived hepatocytes in the replacement of injured liver. PNAS 100:11850-11853. 2003 [PMID: 12920184]
• Kootstra, NA et al. Abrogation of postentry resctriction of HIV-1-based lentiviral vector transduction in simian cells. PNAS 100:1298-1303. 2003 [PMID: 12547912]
• Meyerzon M, Fridolfsson HN, Ly N et al. UNC-83 is a nuclear-specific cargo adaptor for kinesin-1-mediated nuclear migration. Development;136(16):2725-2733. 2009 [PMID: 9605495]

Reviews for GFP Antibody (NB600-308) (18)

Average Rating: 4.5

(Based on 18 reviews)

We have 18 reviews tested in 4 species: Human, Mouse, Rat, Other.

reviewed by: Anonymous IHC-Fr Mouse 04/12/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Adult mouse brain tissue
• Species: Mouse
• Lot: 35459

Comments

• Comments: Dilutions 1:400

reviewed by: Anonymous ICC Mouse 03/22/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: FFPE
• Species: Mouse

Comments

• Comments: Trypsin retrieval

reviewed by: Jean-Paul Paluzzi WB Human 03/10/2017

Summary

• Application: Western Blot
• Sample Tested: whole cellular protein and fractionated (soluble and membrane protein)
• Species: Human

Comments

• Comments: Human embryonic kidney 293T
  HEK293T cells

reviewed by: Wenchao Zhou Human 03/02/2017

Summary

• Sample Tested: Human Xenograft Tumor
• Species: Human

reviewed by: Anonymous IF Other 07/27/2016

Summary

• Application: Immunofluorescence
• Sample Tested: Pig TM cells
• Species: Other

Blocking

• Blocking Details: -

Details

• Detection Notes: -

reviewed by: Anonymous IF Mouse 10/10/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse, 3T3 cells
• Species: Mouse
• Lot: 28839

Blocking

• Blocking Details: 1% donkey serum+1% BSA+0.1% Triton X-100 in 1xPBS (Blocking Buffer)

Primary Anitbody

• Dilution Ratio: 1:500, 1 hr at room temperature. Primary antibody was diluted in blocking buffer

Secondary Antibody

• Secondary Description: Alexa Fluor 488 conjugated to Donkey anti-rabbit IgG
• Secondary Manufacturer Cat#: A-21206
• Secondary Concentration: 1:500

Details

• Detection Notes: Images were obtained on a Leica SP8 confocal microscope with 63X objective and a 6x zoom factor
• Fixation Details: Methanol fixation for 15 min at -20 deg
• Wash Description: 1x PBS wash, 5 min each wash, 3 washes

reviewed by: Anonymous WB Human 10/10/2015

Summary

• Application: Western Blot
• Sample Tested: HEK293T whole cell extract
• Species: Human
• Lot: 28839

Blocking

• Blocking Details: 5% milk in TBS-T (0.1% Tween)

Primary Anitbody

• Dilution Ratio: 1:5000, overnight at 4 deg, primary antibody was diluted in TBS-T (0.1% Tween) with 5% milk

Secondary Antibody

• Secondary Description: Peroxidase conjugated Donkey anti-Rabbit IgG (H+L)
• Secondary Manufacturer Cat#: 711-035-152
• Secondary Concentration: 0.04 µg/ml

Details

• Detection Notes: ECL, Film, 5 sec, GFP tagged constructs

Comments

• Comments: This antibody works very well in Western Blotting

reviewed by: Anonymous IP Other 04/02/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: S2 Cells (Drosophila)
• Species: Other

Blocking

• Blocking Details: BSA 1%, Incubation time: 3h at 4°C

Primary Anitbody

• Dilution Ratio: polyclonal anti-GFP antibody, 1:1000; Incubation time: 3h at 4°C

Details

• Detection Notes: Detection was perfomed in Wersten Blot by using polyclonal anti-GFP antibody, 1:1000. An load control was used as control.
• Immunoprecipitation Details: protein-A Sepharose beads; Incubation time: 3h at 4°C; Beads were washed 3 times with lysis buffer

reviewed by: Megan Cleveland IF Mouse 11/25/2014

Summary

• Application: Immunofluorescence
• Sample Tested: transgenic mouse pancreas
• Species: Mouse

Blocking

• Blocking Details: block 1 hr at RT with 10% FBS PBST

Primary Anitbody

• Dilution Ratio: 1/100 in 10% FBS PBST, incubated overnight at 4 C

Secondary Antibody

• Secondary Description: Goat anti-rabbit Cy3 conjugated
• Secondary Manufacturer Cat#: Jackson Immunoresearch Laboratories 111-166-045
• Secondary Concentration: 1/200

Details

• Detection Notes: Zeiss fluorescent microscope 8s; this transgenic mouse was the positive control; neg con is secondary only/negative islet tissue
• Fixation Details: 4% PFA for 4 hours, cryopreserve in 30% sucrose overnight, permeabilize with PBST 15 min at RT
• Wash Description: wash 3x in PBST, 10 min each

Comments

• Comments: the blue region in the center is an islet which does not express GFP and is negative, as expected

reviewed by: Anonymous IHC-P 10/05/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Lot: 28839
• Comments: Excellent product, works well on Paraffin embedded and PFA/Sucrose fixed sections. Highly recommended.

reviewed by: Samantha Garcia IHC-Fr Mouse 11/06/2013

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Transgenic mouse pancreas in OCT
• Species: Mouse
• Lot: 24437
• Comments: The staining with the antibody at 1:100 was very bright. I could have increased the dilution to 1:300.

Blocking

• Blocking Details: 10% FBS in PBST

Primary Anitbody

• Dilution Ratio: 1:100 in blocking buffer

Comments

• Comments: The staining with the antibody at 1:100 was very bright. I could have increased the dilution to 1:300.

reviewed by: Mladen Yovchev IF Rat 07/13/2012

Summary

• Application: Immunofluorescence
• Sample Tested: Rat liver
• Species: Rat
• Lot: 24437
• Comments: This is the only antibody I found on the market that detects EGFP. It is very strong and specific. I have no issues with background. I have used at least 5 vials and there are some lot to lot differences but overall the performance is the same.

Blocking

• Blocking Details: blocking 1h in PBS/1% donkey serum/1% BSA

Primary Anitbody

• Dilution Ratio: 1:400 dilution, incubation for 2h at RT in blocking buffer

Secondary Antibody

• Secondary Description: anti-rabbit IgG DyLight488
• Secondary Manufacturer Cat#: Vector DI-1488
• Secondary Concentration: Dilution 1:100

Details

• Fixation Details: 4% PFA for 10 min than 0.3% Triton X100 in PBS for 10 min on ice
• Wash Description: PBS/0.05% Tween20, 2x 10 min each

Comments

• Comments: This is the only antibody I found on the market that detects EGFP. It is very strong and specific. I have no issues with background. I have used at least 5 vials and there are some lot to lot differences but overall the performance is the same.

reviewed by: Marisa Carbonaro IP Human 04/24/2012

Summary

• Application: Immunoprecipitation
• Sample Tested: Hela whole cell lysate
• Species: Human

reviewed by: Shao-Win Wang WB Other 11/15/2011

Summary

• Application: Western Blot
• Sample Tested: Yeast
• Species: Other

Blocking

• Blocking Details: Blocking Buffer: 5% Milk in BSA

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:2000, Primary Ab Incubation Time: 10 minutes

Secondary Antibody

• Secondary Description: Secondary Ab: anti-rabbit, Secondary Ab Dilution Ratio: 1:5000

Details

• Detection Notes: Detection Method: ECL

reviewed by: Anonymous IHC Mouse 09/28/2011

Summary

• Application: Immunohistochemistry
• Sample Tested: IHC on parrafin sections (mouse)
• Species: Mouse
• Comments: This is the only GFP antibody on the market that we could find for paraffin sections. It requires special retrieval conditions: 100mm tris pH10, in pressure with 15 minutes of high pressure and two hours of total heat.
• Pub Med ID: 21311774

Comments

• Comments: This is the only GFP antibody on the market that we could find for paraffin sections. It requires special retrieval conditions: 100mm tris pH10, in pressure with 15 minutes of high pressure and two hours of total heat.

reviewed by: Huang Sheng Chieh IF Mouse 07/20/2011

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse Retinal Cells
• Species: Mouse
• Comments: Transgenic mice have FITC expresion in retina inner nuclear layer.

Blocking

• Blocking Details: Blocking Buffer: 5% goat serum, Blocking Time: overnight, Blocking Temp: 4 degrees Celsius

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:200

Secondary Antibody

• Secondary Description: Secondary Ab: Alexa Fluor 488 goat anti-rabbit IgG (H&L)

Details

• Detection Notes: Detection Visualization Method: Leica TCS SP5
• Fixation Details: Sample Preparation: 4% PFA treated slides

Comments

• Comments: Transgenic mice have FITC expresion in retina inner nuclear layer.

reviewed by: Astrid Spannhoff WB Other 11/23/2010

Summary

• Application: Western Blot
• Sample Tested: Pig ear fibroblasts, Sample Amount: 10 ul
• Species: Other

Blocking

• Blocking Details: Blocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: 20 degrees Celsius

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:500, Incubation Dilution Buffer: 5% Milk in PBST, Incubation Time: 1hr, Incubation Temp: 20C

Secondary Antibody

• Secondary Description: Secondary Ab: anti-rabbit IgG, Secondary Ab Dilution Ratio: 1:5000
• Secondary Manufacturer Cat#: NA9340

Details

• Detection Notes: Detection Method: ECL, Exposure Time: 1 minute, Specific Bands: 27 kDa

reviewed by: Dianne Soprano WB Mouse 09/23/2010

Summary

• Application: Western Blot
• Sample Tested: P19 cells transfectedwith a plasmid DNA expressing a GFP fusion protein, Sample Amount: 20 micrograms
• Species: Mouse
• Comments: The GFP antibody worked just fine. We obtained strong bands with little to no background. We used standard procedure as recommended by LiCor for using with the Odyssey Instrument for detection.

Comments

• Comments: The GFP antibody worked just fine. We obtained strong bands with little to no background. We used standard procedure as recommended by LiCor for using with the Odyssey Instrument for detection.

FAQs for GFP Antibody (NB600-308). (Showing 1 - 2 of 2 FAQs).

1. Does the GFP antibody NB600-308 also work against AcGFP (93% identical to EGFP) in IP and Western blot?
   • For our product NB600-308, we have unfortunately not yet tested the product for cross reactivity with AcGFP. However, since the antibody is polyclonal and made to the full length AvGFP sequence, there is a good chance for cross reactivity.
2. Hello, my lab purchased a GFP antibody from Novus in the past and I am now in need of an antibody to detect YFP and CFP as well. The GFP antibody we order has a part number of NB600-308. Would it possibly recognize YPF and CFP as well or will I need to purchase additional antibodies?
   • NB600-308 is polyclonal to full length GFP, hence it will recognize most of its mutants. It will react with YFP and CFP.

Bioinformatics

• Uniprot:
  • P42212()