GFP Antibody

Images

 
Western Blot: GFP Antibody [NB600-308] - Wild type GFP (0.1 ug) was used to spike HeLa whole cell lysate. Load: 30 ug per lane. Primary antibody: GFP antibody at 1:1000 for overnight at 4C. Secondary antibody: IR Dye800 ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-308] - Analysis of GFP in transgenic mouse pancreas in OCT. The blue region in the center is an islet which does not express GFP and is negative. Image from ...read more
Western Blot: GFP Antibody [NB600-308] - analysis of GFP tagged PKA subunits over-expressed in HEK293T cells using GFP antibody. Image from verified customer review.
Immunohistochemistry: GFP Antibody [NB600-308] - Analysis of GFP in mouse retinal cells. Image courtesy of anonymous customer review.
Immunohistochemistry-Paraffin: GFP Antibody [NB600-308] - Staining of GFP+ hepatocytes (brown) transplanted into the liver of a recipient animal using anti-GFP antibody. Cyclin D1 double staining in blue. Image from ...read more
Immunohistochemistry-Frozen: GFP Antibody [NB600-308] - Adult mouse brain tissue. This image was submitted via customer review.
Immunoprecipitation: GFP Antibody [NB600-308] - IP analysis of GFP in S2 Cells (Drosophila). Image from verified customer review.
Immunoprecipitation: GFP Antibody [NB600-308] - Immunoprecipiation experiments showing that GFP-MISP binds endogenous IQGAP1 (upper panel) and MISP binds to IQGAP1 endogenously using specific antibodies (lower panel) in ...read more

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, ELISA, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl, IHC-WhMt, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.99 mg/ml

Order Details

GFP Antibody Summary

Immunogen
This GFP antibody was developed against a fusion protein corresponding to the full length GFP (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Anti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). Primate reactivity reported in scientific literature (PMID: 27723741).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • Electron Microscopy 1:10-1:500
  • ELISA 1:20000-1:120000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:5000
  • Immunohistochemistry 1:200-1:3000
  • Immunohistochemistry-Frozen 1:50-1:250
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
  • Knockdown Validated
Application Notes
Use in Knockdown Validated reported in scientific literature (PMID: 32905777).
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 18 Reviews rated 4.5
using
NB600-308 in the following applications:

Publications
Read Publications using
NB600-308 in the following applications:

Reactivity Notes

No reaction was observed against Human, Mouse serum proteins. Suitable for detecting fusion proteins containing the GFP sequence expressed in Human, Mouse, Rat, C. elegans, Drosophila and in vitro transcription/translation systems and transgenic animals. GFP Transgenic Rat reactivity reported in scientific literature (PMID:25724725). Mouse reactivity reported in multiple pieces of scientific literature.Transgenic C. elegans reactivity reported in scientific literature (PMID: 27110099). Known cross reactivity with wt and all variants such as rGFP, eGFP, S65T-GFP, RS-GFP, YFP and EGFP. Use in Mouse reported in secitific publication PMID: 32765228 Plant reactivity reported in scientific literature (PMID:32896843)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
0.99 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GFP Antibody

  • anti-gfp (green fluorescent protein) pab
  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP immunofluorescence
  • GFP immunoprecipitation
  • GFP mouse
  • GFP staining
  • GFP western blot
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T.,... Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S.,... Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB600-308)(192)

We have publications tested in 6 confirmed species: Human, Mouse, C. elegans, Drosophila, Non-species specific, Plant.

We have publications tested in 11 applications: ELISA, EM, ICC/IF, IHC, IHC-Fr, IHC-FrFl, IHC-P, IHC-WhMt, IP, KD, WB.


Filter By Application
ELISA
(1)
EM
(1)
ICC/IF
(33)
IHC
(28)
IHC-Fr
(8)
IHC-FrFl
(2)
IHC-P
(8)
IHC-WhMt
(2)
IP
(17)
KD
(1)
WB
(48)
All Applications
Filter By Species
Human
(1)
Mouse
(26)
C. elegans
(9)
Drosophila
(1)
Non-species specific
(1)
Plant
(2)
All Species
Showing Publications 1 - 10 of 192. Show All 192 Publications.
Publications using NB600-308 Applications Species
Tang C, Han J, Dalvi S et al. A human model of Batten disease shows role of CLN3 in phagocytosis at the photoreceptor-RPE interface Communications biology Feb 5 2021 [PMID: 33547385] (WB, Human, Mouse) WB Human, Mouse
González-Prieto R, Eifler-Olivi K, Claessens LA, et al. Global non-covalent SUMO interaction networks reveal SUMO-dependent stabilization of the non-homologous end joining complex Cell reports Jan 26 2021 [PMID: 33503430]
Bland KM, Aharon A, Widener EL, et al. FMRP regulates the subcellular distribution of cortical dendritic spine density in a non-cell-autonomous manner Neurobiology of disease Jan 6 2021 [PMID: 33421563]
Vergarajauregui S, Becker R, Steffen U et al. AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9 eLife Dec 9 2020 [PMID: 33295871] (Non-species specific) Non-species specific
Wolter JM, Mao H, Fragola G et al. Cas9 gene therapy for Angelman syndrome traps Ube3a-ATS long non-coding RNA Nature Oct 21 2020 [PMID: 33087932]
Sade N, Weng F, Tajima H et al. A Cytoplasmic Receptor-like Kinase Contributes to Salinity Tolerance Plants (Basel) Oct 17 2020 [PMID: 33080797]
Im JY, Kim DM, Park H et al. VGLL1 phosphorylation and activation promotes gastric cancer malignancy via TGF-B/ERK/RSK2 signaling Biochim Biophys Acta Mol Cell Res Oct 16 2020 [PMID: 33069758] (WB) WB
Donato C, Kunz L, Castro-Giner F et al. Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells Cell reports Sep 8 2020 [PMID: 32905777] (WB, KD, ICC/IF) WB, KD, ICC/IF
Thagun C, Motoda Y, Kigawa T et al. Simultaneous introduction of multiple biomacromolecules into plant cells using a cell-penetrating peptide nanocarrier Nanoscale Sep 28 2020 [PMID: 32896843] (WB, Plant)

Details:
Nicotiana benthamiana
WB Plant
Kim H, Berens N, Ochandarena N, Philpot B Region and Cell Type Distribution of TCF4 in the Postnatal Mouse Brain Front. Neuroanat. Jul 17 2020 [PMID: 32765228] (ICC/IF, Mouse) ICC/IF Mouse
Show All 192 Publications.

Reviews for GFP Antibody (NB600-308) (18) 4.518

Average Rating: 4.5
(Based on 18 reviews)
We have 18 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using NB600-308:
Filter by Applications
IHC-Fr
(2)
ICC
(1)
WB
(5)
IF
(5)
IP
(2)
IHC-P
(1)
IHC
(1)
All Applications
Filter by Species
Human
(4)
Mouse
(8)
Rat
(1)
Other
(4)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen GFP NB600-308
Enlarge
5
reviewed by:
Anonymous
IHC-Fr Mouse 04/12/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult mouse brain tissue
SpeciesMouse
Lot35459

Comments

CommentsDilutions 1:400
Immunocytochemistry GFP NB600-308
Enlarge
5
reviewed by:
Anonymous
ICC Mouse 03/22/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedFFPE
SpeciesMouse

Comments

CommentsTrypsin retrieval
Western Blot GFP NB600-308
Enlarge
5
reviewed by:
Jean-Paul Paluzzi
WB Human 03/10/2017
View

Summary

ApplicationWestern Blot
Sample Testedwhole cellular protein and fractionated (soluble and membrane protein)
SpeciesHuman

Comments

CommentsHuman embryonic kidney 293T
HEK293T cells
 GFP NB600-308
Enlarge
3
reviewed by:
Wenchao Zhou
Human 03/02/2017
View

Summary

Sample TestedHuman Xenograft Tumor
SpeciesHuman
Immunofluorescence GFP NB600-308
Enlarge
5
reviewed by:
Anonymous
IF Other 07/27/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedPig TM cells
SpeciesOther

Blocking

Blocking Details-

Details

Detection Notes-
Immunofluorescence GFP NB600-308
Enlarge
5
reviewed by:
Anonymous
IF Mouse 10/10/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse, 3T3 cells
SpeciesMouse
Lot28839

Blocking

Blocking Details1% donkey serum+1% BSA+0.1% Triton X-100 in 1xPBS (Blocking Buffer)

Primary Anitbody

Dilution Ratio1:500, 1 hr at room temperature. Primary antibody was diluted in blocking buffer

Secondary Antibody

Secondary DescriptionAlexa Fluor 488 conjugated to Donkey anti-rabbit IgG
Secondary Manufacturer Cat#A-21206
Secondary Concentration1:500

Details

Detection NotesImages were obtained on a Leica SP8 confocal microscope with 63X objective and a 6x zoom factor
Fixation DetailsMethanol fixation for 15 min at -20 deg
Wash Description1x PBS wash, 5 min each wash, 3 washes
Western Blot GFP NB600-308
Enlarge
5
reviewed by:
Anonymous
WB Human 10/10/2015
View

Summary

ApplicationWestern Blot
Sample TestedHEK293T whole cell extract
SpeciesHuman
Lot28839

Blocking

Blocking Details5% milk in TBS-T (0.1% Tween)

Primary Anitbody

Dilution Ratio1:5000, overnight at 4 deg, primary antibody was diluted in TBS-T (0.1% Tween) with 5% milk

Secondary Antibody

Secondary DescriptionPeroxidase conjugated Donkey anti-Rabbit IgG (H+L)
Secondary Manufacturer Cat#711-035-152
Secondary Concentration0.04 µg/ml

Details

Detection NotesECL, Film, 5 sec, GFP tagged constructs

Comments

CommentsThis antibody works very well in Western Blotting
Immunoprecipitation GFP NB600-308
Enlarge
5
reviewed by:
Anonymous
IP Other 04/02/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedS2 Cells (Drosophila)
SpeciesOther

Blocking

Blocking DetailsBSA 1%, Incubation time: 3h at 4°C

Primary Anitbody

Dilution Ratiopolyclonal anti-GFP antibody, 1:1000; Incubation time: 3h at 4°C

Details

Detection NotesDetection was perfomed in Wersten Blot by using polyclonal anti-GFP antibody, 1:1000. An load control was used as control.
Immunoprecipitation Detailsprotein-A Sepharose beads; Incubation time: 3h at 4°C; Beads were washed 3 times with lysis buffer
Immunofluorescence GFP NB600-308
Enlarge
4
reviewed by:
Megan Cleveland
IF Mouse 11/25/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedtransgenic mouse pancreas
SpeciesMouse

Blocking

Blocking Detailsblock 1 hr at RT with 10% FBS PBST

Primary Anitbody

Dilution Ratio1/100 in 10% FBS PBST, incubated overnight at 4 C

Secondary Antibody

Secondary DescriptionGoat anti-rabbit Cy3 conjugated
Secondary Manufacturer Cat#Jackson Immunoresearch Laboratories 111-166-045
Secondary Concentration1/200

Details

Detection NotesZeiss fluorescent microscope 8s; this transgenic mouse was the positive control; neg con is secondary only/negative islet tissue
Fixation Details4% PFA for 4 hours, cryopreserve in 30% sucrose overnight, permeabilize with PBST 15 min at RT
Wash Descriptionwash 3x in PBST, 10 min each

Comments

Commentsthe blue region in the center is an islet which does not express GFP and is negative, as expected
Immunohistochemistry-Paraffin GFP NB600-308
Enlarge
5
reviewed by:
Anonymous
IHC-P 10/05/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Lot28839
CommentsExcellent product, works well on Paraffin embedded and PFA/Sucrose fixed sections. Highly recommended.
Immunohistochemistry-Frozen GFP NB600-308
Enlarge
4
reviewed by:
Samantha Garcia
IHC-Fr Mouse 11/06/2013
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedTransgenic mouse pancreas in OCT
SpeciesMouse
Lot24437
CommentsThe staining with the antibody at 1:100 was very bright. I could have increased the dilution to 1:300.

Blocking

Blocking Details10% FBS in PBST

Primary Anitbody

Dilution Ratio1:100 in blocking buffer

Comments

CommentsThe staining with the antibody at 1:100 was very bright. I could have increased the dilution to 1:300.
Immunofluorescence GFP NB600-308
Enlarge
5
reviewed by:
Mladen Yovchev
IF Rat 07/13/2012
View

Summary

ApplicationImmunofluorescence
Sample TestedRat liver
SpeciesRat
Lot24437
CommentsThis is the only antibody I found on the market that detects EGFP. It is very strong and specific. I have no issues with background. I have used at least 5 vials and there are some lot to lot differences but overall the performance is the same.

Blocking

Blocking Detailsblocking 1h in PBS/1% donkey serum/1% BSA

Primary Anitbody

Dilution Ratio1:400 dilution, incubation for 2h at RT in blocking buffer

Secondary Antibody

Secondary Descriptionanti-rabbit IgG DyLight488
Secondary Manufacturer Cat#Vector DI-1488
Secondary ConcentrationDilution 1:100

Details

Fixation Details4% PFA for 10 min than 0.3% Triton X100 in PBS for 10 min on ice
Wash DescriptionPBS/0.05% Tween20, 2x 10 min each

Comments

CommentsThis is the only antibody I found on the market that detects EGFP. It is very strong and specific. I have no issues with background. I have used at least 5 vials and there are some lot to lot differences but overall the performance is the same.
Immunoprecipitation GFP NB600-308
Enlarge
4
reviewed by:
Marisa Carbonaro
IP Human 04/24/2012
View

Summary

ApplicationImmunoprecipitation
Sample TestedHela whole cell lysate
SpeciesHuman
  4
reviewed by:
Shao-Win Wang
WB Other 11/15/2011
View

Summary

ApplicationWestern Blot
Sample TestedYeast
SpeciesOther

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:2000, Primary Ab Incubation Time: 10 minutes

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-rabbit, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: ECL
  5
reviewed by:
Anonymous
IHC Mouse 09/28/2011
View

Summary

ApplicationImmunohistochemistry
Sample TestedIHC on parrafin sections (mouse)
SpeciesMouse
CommentsThis is the only GFP antibody on the market that we could find for paraffin sections. It requires special retrieval conditions: 100mm tris pH10, in pressure with 15 minutes of high pressure and two hours of total heat.
Pub Med ID21311774

Comments

CommentsThis is the only GFP antibody on the market that we could find for paraffin sections. It requires special retrieval conditions: 100mm tris pH10, in pressure with 15 minutes of high pressure and two hours of total heat.
Immunofluorescence GFP NB600-308
Enlarge
4
reviewed by:
Huang Sheng Chieh
IF Mouse 07/20/2011
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse Retinal Cells
SpeciesMouse
CommentsTransgenic mice have FITC expresion in retina inner nuclear layer.

Blocking

Blocking DetailsBlocking Buffer: 5% goat serum, Blocking Time: overnight, Blocking Temp: 4 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:200

Secondary Antibody

Secondary DescriptionSecondary Ab: Alexa Fluor 488 goat anti-rabbit IgG (H&L)

Details

Detection NotesDetection Visualization Method: Leica TCS SP5
Fixation DetailsSample Preparation: 4% PFA treated slides

Comments

CommentsTransgenic mice have FITC expresion in retina inner nuclear layer.
  4
reviewed by:
Astrid Spannhoff
WB Other 11/23/2010
View

Summary

ApplicationWestern Blot
Sample TestedPig ear fibroblasts, Sample Amount: 10 ul
SpeciesOther

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: 20 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Incubation Dilution Buffer: 5% Milk in PBST, Incubation Time: 1hr, Incubation Temp: 20C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-rabbit IgG, Secondary Ab Dilution Ratio: 1:5000
Secondary Manufacturer Cat#NA9340

Details

Detection NotesDetection Method: ECL, Exposure Time: 1 minute, Specific Bands: 27 kDa
  4
reviewed by:
Dianne Soprano
WB Mouse 09/23/2010
View

Summary

ApplicationWestern Blot
Sample TestedP19 cells transfectedwith a plasmid DNA expressing a GFP fusion protein, Sample Amount: 20 micrograms
SpeciesMouse
CommentsThe GFP antibody worked just fine. We obtained strong bands with little to no background. We used standard procedure as recommended by LiCor for using with the Odyssey Instrument for detection.

Comments

CommentsThe GFP antibody worked just fine. We obtained strong bands with little to no background. We used standard procedure as recommended by LiCor for using with the Odyssey Instrument for detection.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFP Antibody (NB600-308). (Showing 1 - 2 of 2 FAQs).

  1. Does the GFP antibody NB600-308 also work against AcGFP (93% identical to EGFP) in IP and Western blot?
    • For our product NB600-308, we have unfortunately not yet tested the product for cross reactivity with AcGFP. However, since the antibody is polyclonal and made to the full length AvGFP sequence, there is a good chance for cross reactivity.
  2. Hello, my lab purchased a GFP antibody from Novus in the past and I am now in need of an antibody to detect YFP and CFP as well. The GFP antibody we order has a part number of NB600-308. Would it possibly recognize YPF and CFP as well or will I need to purchase additional antibodies?
    • NB600-308 is polyclonal to full length GFP, hence it will recognize most of its mutants. It will react with YFP and CFP.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-308AF350
Alexa Fluor 405 NB600-308AF405
Alexa Fluor 488 NB600-308AF488
Alexa Fluor 532 NB600-308AF532
Alexa Fluor 594 NB600-308AF594
Alexa Fluor 647 NB600-308AF647
Alexa Fluor 700 NB600-308AF700
Alexa Fluor 750 NB600-308AF750
Allophycocyanin NB600-308APC
Allophycocyanin/Cy7 NB600-308APCCY7
Biotin NB600-308B
DyLight 350 NB600-308UV
DyLight 405 NB600-308V
DyLight 488 NB600-308G
DyLight 550 NB600-308R
DyLight 594 NB600-308DL594
DyLight 650 NB600-308C
DyLight 680 NB600-308FR
DyLight 755 NB600-308IR
FITC NB600-308F
HRP NB600-308H
Janelia Fluor 549 NB600-308JF549
Janelia Fluor 646 NB600-308JF646
PE NB600-308PE
PE/Atto594 NB600-308PEATT594
PE/Cy5.5 NB600-308PECY55
PE/Cy7 NB600-308PECY7
PerCP NB600-308PCP

Additional GFP Products

Research Areas for GFP Antibody (NB600-308)

Find related products by research area.

Blogs on GFP. Showing 1-10 of 12 blog posts - Show all blog posts.

Autophagy and RAS signaling: Clinical implications
By Christina Towers, PhD The cellular recycling process known as autophagy is currently being targeted in over 60 clinical trials focused on treating different types of cancer1. To date, the only autophagy-targeted...  Read full blog post.

Neurovascular signaling for repair enhances brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.

Make each cell count: How to assess autophagy using flow cytometry
Kristy R. Howell, PhDThe cellular recycling process known as autophagy may be induced by a variety of conditions including reduced nutrient availability, serum starvation and pharmacological agents (e.g., Rapamyci...  Read full blog post.

How to visualize autophagy by microscopy
By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to...  Read full blog post.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

Best way to quantitatively measure Autophagic Flux
By Christina Towers, PhD Autophagy is a stress-induced cellular recycling process that plays an important physiological role in many diseases. It is induced by a variety of stimuli, both intracellular and extracel...  Read full blog post.

Animal Models to Study Autophagy
By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

GFP - Be Green!
Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

GFP (Green Fluorescent Protein) Bringing Light to Life
GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.5
5
10
4
7
3
1
2
0
1
0

Anonymous
04/12/2018
Application: IHC-Fr
Species: Mouse

Anonymous
03/22/2017
Application: ICC
Species: Mouse

Jean-Paul Paluzzi
03/10/2017
Application: WB
Species: Human

Bioinformatics

Uniprot