GFP Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-308] - Analysis of GFP in transgenic mouse pancreas in OCT. The blue region in the center is an islet which does not express GFP and is negative. Image from ...read more
Western Blot: GFP Antibody [NB600-308] - analysis of GFP tagged PKA subunits over-expressed in HEK293T cells using GFP antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: GFP Antibody [NB600-308] - Staining of GFP+ hepatocytes (brown) transplanted into the liver of a recipient animal using anti-GFP antibody. Cyclin D1 double staining in blue. Image from ...read more
Western Blot: GFP Antibody [NB600-308] - Western Blot of GFP antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: CHO/K1 Lysate. Lane 4: MDA-MB-231. Lane 5: A431 Lysate. Lane 6: ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-308]
Immunohistochemistry: GFP Antibody [NB600-308] - Analysis of GFP in mouse retinal cells. Image courtesy of anonymous customer review.
Western Blot: GFP Antibody [NB600-308] - Western Blot of GFP antibody. Lane 1: Wild type GFP (0.1 ug) was used to spike HeLa whole cell lysate. Lane 2: none. Load: 30 ug per lane.Primary antibody: GFP antibody at 1:1000 ...read more
Western Blot: GFP Antibody [NB600-308] - Western Blot of GFP antibody. Lane 1: 293FT cells transfected with CDK4 dominant negative (C-).Lane 2: 293FT cells poitive control (C+).Load: 25 ug per lane.Primary antibody: GFP ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB600-308] - Immuno-microscopy of GFP antibody. Monocyte derived dendritic cells and dermal macrophages were challenged and directly visualized with eGFP labeled ...read more
Immunohistochemistry-Frozen: GFP Antibody [NB600-308] - Adult mouse brain tissue. This image was submitted via customer review.
Immunoprecipitation: GFP Antibody [NB600-308] - IP analysis of GFP in S2 Cells (Drosophila). Image from verified customer review.
Immunoprecipitation: GFP Antibody [NB600-308] - Immunoprecipitation experiments showing that GFP-MISP binds endogenous IQGAP1 (upper panel) and MISP binds to IQGAP1 endogenously using specific antibodies (lower panel) ...read more

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, DB, ELISA, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl, IHC-WhMt, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GFP Antibody Summary

Immunogen
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Anti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). Primate reactivity reported in scientific literature (PMID: 27723741).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot
  • Electron Microscopy 1:10-1:500
  • ELISA 1:20000-1:120000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:500-1:5000
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:200-1:3000
  • Immunohistochemistry-Frozen 1:50-1:250
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:500-1:5000
Application Notes
Use in Immunoprecipitation reported in scientific literature (PMID:34887587).Use in DB reported in scientific literature (PMID:34242364). This antibody is designed to detect GFP and its variants. GFP antibody can be used to detect GFP by ELISA (sandwich or capture). for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. This Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP. This antibody can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli). and eukaryotic (CHO cells). expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
Use in Knockdown Validated reported in scientific literature (PMID: 32905777)...
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 18 Reviews rated 4.5
using
NB600-308 in the following applications:

Publications
Read Publications using
NB600-308 in the following applications:

Reactivity Notes

No reaction was observed against Human, Mouse serum proteins. Suitable for detecting fusion proteins containing the GFP sequence expressed in Human, Mouse, Rat, C. elegans, Drosophila and in vitro transcription/translation systems and transgenic animals. GFP Transgenic Rat reactivity reported in scientific literature (PMID:25724725). Mouse reactivity reported in multiple pieces of scientific literature.Transgenic C. elegans reactivity reported in scientific literature (PMID: 27110099). Known cross reactivity with wt and all variants such as rGFP, eGFP, S65T-GFP, RS-GFP, YFP and EGFP. Use in Mouse reported in secitific publication PMID: 32765228 Plant reactivity reported in scientific literature (PMID:32896843)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Notes

This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria)

GFP antibody is stable for several weeks at 4C as an undiluted liquid.

Alternate Names for GFP Antibody

  • anti-gfp (green fluorescent protein) pab
  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP immunofluorescence
  • GFP immunoprecipitation
  • GFP mouse
  • GFP staining
  • GFP western blot
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T., . . . Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S., . . . Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB600-308)(223)

We have publications tested in 4 confirmed species: Mouse, C. elegans, Drosophila, Non-species specific.

We have publications tested in 14 applications: DB, ELISA, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-FrFl, IHC-P, IHC-WhMt, IP, ISH, KD, WB.


Filter By Application
DB
(1)
ELISA
(1)
EM
(1)
Flow
(2)
ICC/IF
(38)
IHC
(37)
IHC-Fr
(7)
IHC-FrFl
(2)
IHC-P
(10)
IHC-WhMt
(2)
IP
(18)
ISH
(1)
KD
(1)
WB
(55)
All Applications
Filter By Species
Mouse
(2)
C. elegans
(3)
Drosophila
(1)
Non-species specific
(1)
All Species
Showing Publications 1 - 10 of 223. Show All 223 Publications.
Publications using NB600-308 Applications Species
Osaki Y, Manolopoulou M, Ivanova AV et al. Blocking cell cycle progression through CDK4/6 protects against chronic kidney disease JCI insight Jun 22 2022 [PMID: 35730565] (Flow, IHC) Flow, IHC
Feng C, Wang Y, Zha X et al. Cold-sensitive ventromedial hypothalamic neurons control homeostatic thermogenesis and social interaction-associated hyperthermia Cell metabolism Jun 7 2022 [PMID: 35675799] (IHC) IHC
Ma L, Chen C, Liu D et al. Apoptotic extracellular vesicles are metabolized regulators nurturing the skin and hair Bioactive materials Jan 1 2023 [PMID: 35600968] (ICC/IF) ICC/IF
Law SSY, Liou G, Nagai Y et al. Polymer-coated carbon nanotube hybrids with functional peptides for gene delivery into plant mitochondria Nature communications May 16 2022 [PMID: 35577779] (WB) WB
Gu T, Hao D, Woo J et al. The disordered N-terminal domain of DNMT3A recognizes H2AK119ub and is required for postnatal development Nature genetics May 1 2022 [PMID: 35534561] (WB) WB
Lao M, Hurtado A, de Castro AC et al. Sox9 is required for nail bed differentiation and digit tip regeneration The Journal of investigative dermatology Apr 7 2022 [PMID: 35398376]
Thagun C, Horii y, Mori M et al. Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts ACS nano Feb 23 2022 [PMID: 35195009] (WB) WB
Kakoty V, C SK, Yang CH et al. Neuroprotective Effect of Lentivirus-Mediated FGF21 Gene Delivery in Experimental Alzheimer's Disease is Augmented when Concerted with Rapamycin Molecular neurobiology Feb 10 2022 [PMID: 35142986]
Elbaz M, Callado Perez A, Demers M et al. A vibrissa pathway that activates the limbic system eLife Feb 10 2022 [PMID: 35142608] (IHC) IHC
Sun J, Yoon J, Lee M et al. Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development Cell reports Feb 1 2022 [PMID: 35108539] (ICC/IF) ICC/IF
Show All 223 Publications.

Reviews for GFP Antibody (NB600-308) (18) 4.518

Average Rating: 4.5
(Based on 18 reviews)
We have 18 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using NB600-308:
Filter by Applications
Immunohistochemistry-Frozen
(2)
Immunocytochemistry
(1)
Western Blot
(5)
Immunofluorescence on tissues.
(1)
Immunofluorescence
(5)
Immunoprecipitation
(2)
Immunohistochemistry-Paraffin
(1)
Immunohistochemistry
(1)
All Applications
Filter by Species
Human
(4)
Mouse
(8)
Rat
(1)
Other
(4)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen GFP NB600-308
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5
reviewed by:
Abhijit Nirwane
Immunohistochemistry-Frozen Mouse 04/12/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult mouse brain tissue
SpeciesMouse
Lot35459

Comments

CommentsDilutions 1:400
Immunocytochemistry GFP NB600-308
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5
reviewed by:
Priyanka Manandhar
Immunocytochemistry Mouse 03/22/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedFFPE
SpeciesMouse

Comments

CommentsTrypsin retrieval
Western Blot GFP NB600-308
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5
reviewed by:
Verified Customer
Western Blot Human 03/10/2017
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Summary

ApplicationWestern Blot
Sample Testedwhole cellular protein and fractionated (soluble and membrane protein)
SpeciesHuman

Comments

CommentsHuman embryonic kidney 293T
HEK293T cells
Other GFP NB600-308
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3
reviewed by:
Verified Customer
Immunofluorescence on tissues. Human 03/02/2017
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Summary

ApplicationOther
Sample TestedHuman Xenograft Tumor
SpeciesHuman
Immunofluorescence GFP NB600-308
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5
reviewed by:
Anonymous -
Immunofluorescence Other 07/27/2016
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Summary

ApplicationImmunofluorescence
Sample TestedPig TM cells
SpeciesOther
Immunofluorescence GFP NB600-308
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5
reviewed by:
Ganesh Pusapati
Immunofluorescence Mouse 10/10/2015
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Summary

ApplicationImmunofluorescence
Sample TestedMouse, 3T3 cells
SpeciesMouse
Lot28839
Western Blot GFP NB600-308
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5
reviewed by:
Ganesh Pusapati
Western Blot Human 10/10/2015
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Summary

ApplicationWestern Blot
Sample TestedHEK293T whole cell extract
SpeciesHuman
Lot28839
Immunoprecipitation GFP NB600-308
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5
reviewed by:
Marcio Fontenele
Immunoprecipitation Other 04/02/2015
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Summary

ApplicationImmunoprecipitation
Sample TestedS2 Cells (Drosophila)
SpeciesOther
Immunofluorescence GFP NB600-308
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4
reviewed by:
Verified Customer
Immunofluorescence Mouse 11/25/2014
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Summary

ApplicationImmunofluorescence
Sample Testedtransgenic mouse pancreas
SpeciesMouse
Immunohistochemistry-Paraffin GFP NB600-308
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5
reviewed by:
Fabio Marongiu
Immunohistochemistry-Paraffin 10/05/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Lot28839
Immunohistochemistry-Frozen GFP NB600-308
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4
reviewed by:
Verified Customer
Immunohistochemistry-Frozen Mouse 11/06/2013
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedTransgenic mouse pancreas in OCT
SpeciesMouse
Lot24437
Immunofluorescence GFP NB600-308
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5
reviewed by:
Verified Customer
Immunofluorescence Rat 07/13/2012
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Summary

ApplicationImmunofluorescence
Sample TestedRat liver
SpeciesRat
Lot24437
Immunoprecipitation GFP NB600-308
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4
reviewed by:
Verified Customer
Immunoprecipitation Human 04/24/2012
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Summary

ApplicationImmunoprecipitation
Sample TestedHela whole cell lysate
SpeciesHuman
  4
reviewed by:
Verified Customer
Western Blot Other 11/15/2011
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Summary

ApplicationWestern Blot
Sample TestedYeast
SpeciesOther
  5
reviewed by:
Anna Means
Immunohistochemistry Mouse 09/28/2011
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Summary

ApplicationImmunohistochemistry
Sample TestedIHC on parrafin sections (mouse)
SpeciesMouse
Immunofluorescence GFP NB600-308
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4
reviewed by:
Verified Customer
Immunofluorescence Mouse 07/20/2011
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Summary

ApplicationImmunofluorescence
Sample TestedMouse Retinal Cells
SpeciesMouse
  4
reviewed by:
Verified Customer
Western Blot Other 11/23/2010
View

Summary

ApplicationWestern Blot
Sample TestedPig ear fibroblasts, Sample Amount: 10 ul
SpeciesOther
  4
reviewed by:
Verified Customer
Western Blot Mouse 09/23/2010
View

Summary

ApplicationWestern Blot
Sample TestedP19 cells transfectedwith a plasmid DNA expressing a GFP fusion protein, Sample Amount: 20 micrograms
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFP Antibody (NB600-308). (Showing 1 - 2 of 2 FAQs).

  1. Does the GFP antibody NB600-308 also work against AcGFP (93% identical to EGFP) in IP and Western blot?
    • For our product NB600-308, we have unfortunately not yet tested the product for cross reactivity with AcGFP. However, since the antibody is polyclonal and made to the full length AvGFP sequence, there is a good chance for cross reactivity.
  2. Hello, my lab purchased a GFP antibody from Novus in the past and I am now in need of an antibody to detect YFP and CFP as well. The GFP antibody we order has a part number of NB600-308. Would it possibly recognize YPF and CFP as well or will I need to purchase additional antibodies?
    • NB600-308 is polyclonal to full length GFP, hence it will recognize most of its mutants. It will react with YFP and CFP.

Secondary Antibodies

 

Isotype Controls

Additional GFP Products

Research Areas for GFP Antibody (NB600-308)

Find related products by research area.

Blogs on GFP. Showing 1-10 of 13 blog posts - Show all blog posts.

Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

Autophagy and RAS signaling: Clinical implications
By Christina Towers, PhD The cellular recycling process known as autophagy is currently being targeted in over 60 clinical trials focused on treating different types of cancer1. To date, the only autophagy-targeted ...  Read full blog post.

Neurovascular signaling for repair enhances brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.

Make each cell count: How to assess autophagy using flow cytometry
Kristy R. Howell, PhDThe cellular recycling process known as autophagy may be induced by a variety of conditions including reduced nutrient availability, serum starvation and pharmacological agents (e.g., Rapamyci...  Read full blog post.

How to visualize autophagy by microscopy
By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to...  Read full blog post.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.

Best way to quantitatively measure Autophagic Flux
By Christina Towers, PhD Autophagy is a stress-induced cellular recycling process that plays an important physiological role in many diseases. It is induced by a variety of stimuli, both intracellular and extracel...  Read full blog post.

Animal Models to Study Autophagy
By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

GFP - Be Green!
Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.
Omicron Variant Antibodies

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Recent Reviews

4.5
5
10
4
7
3
1
2
0
1
0

Abhijit Nirwane
04/12/2018
Application: Immunohistochemistry-Frozen
Species: Mouse

Priyanka Manandhar
03/22/2017
Application: Immunocytochemistry
Species: Mouse

Verified Customer
03/10/2017
Application: Western Blot
Species: Human

Bioinformatics

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