Reactivity | All-NASpecies Glossary |
Applications | WB, ELISA, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl, IHC-WhMt, KD |
Clonality | Polyclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Concentration | 0.99 mg/ml |
Immunogen | This GFP antibody was developed against a fusion protein corresponding to the full length GFP (246aa) derived from the jellyfish Aequorea victoria. |
Specificity | Anti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). Primate reactivity reported in scientific literature (PMID: 27723741). |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
|
|
Application Notes | Use in Knockdown Validated reported in scientific literature (PMID: 32905777). |
|
Theoretical MW | 27 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
Reviewed Applications |
|
|
Publications |
|
Storage | Store at -20C. Avoid freeze-thaw cycles. |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Preservative | 0.01% Sodium Azide |
Concentration | 0.99 mg/ml |
Purity | Immunogen affinity purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Anonymous |
IHC-Fr | Mouse | 04/12/2018 |
Summary
Comments
|
||||||||||||||||||||||||||
Enlarge |
reviewed by:
Anonymous |
ICC | Mouse | 03/22/2017 |
Summary
Comments
|
||||||||||||||||||||||||||
Enlarge |
reviewed by:
Jean-Paul Paluzzi |
WB | Human | 03/10/2017 |
Summary
Comments
|
||||||||||||||||||||||||||
Enlarge |
reviewed by:
Wenchao Zhou |
Human | 03/02/2017 |
Summary
|
|||||||||||||||||||||||||||
Enlarge |
reviewed by:
Anonymous |
IF | Other | 07/27/2016 |
Summary
Blocking
Details
|
||||||||||||||||||||||||||
Enlarge |
reviewed by:
Anonymous |
IF | Mouse | 10/10/2015 |
Summary
Blocking
Primary Anitbody
Secondary Antibody
Details
|
||||||||||||||||||||||||||
Enlarge |
reviewed by:
Anonymous |
WB | Human | 10/10/2015 |
Summary
Blocking
Primary Anitbody
Secondary Antibody
Details
Comments
|
||||||||||||||||||||||||||
Enlarge |
reviewed by:
Anonymous |
IP | Other | 04/02/2015 |
Summary
Blocking
Primary Anitbody
Details
|
||||||||||||||||||||||||||
Enlarge |
reviewed by:
Megan Cleveland |
IF | Mouse | 11/25/2014 |
Summary
Blocking
Primary Anitbody
Secondary Antibody
Details
Comments
|
||||||||||||||||||||||||||
Enlarge |
reviewed by:
Anonymous |
IHC-P | 10/05/2014 |
Summary
|
|||||||||||||||||||||||||||
Enlarge |
reviewed by:
Samantha Garcia |
IHC-Fr | Mouse | 11/06/2013 |
Summary
Blocking
Primary Anitbody
Comments
|
||||||||||||||||||||||||||
Enlarge |
reviewed by:
Mladen Yovchev |
IF | Rat | 07/13/2012 |
Summary
Blocking
Primary Anitbody
Secondary Antibody
Details
Comments
|
||||||||||||||||||||||||||
Enlarge |
reviewed by:
Marisa Carbonaro |
IP | Human | 04/24/2012 |
Summary
|
||||||||||||||||||||||||||
reviewed by:
Shao-Win Wang |
WB | Other | 11/15/2011 |
Summary
Blocking
Primary Anitbody
Secondary Antibody
Details
|
|||||||||||||||||||||||||||
reviewed by:
Anonymous |
IHC | Mouse | 09/28/2011 |
Summary
Comments
|
|||||||||||||||||||||||||||
Enlarge |
reviewed by:
Huang Sheng Chieh |
IF | Mouse | 07/20/2011 |
Summary
Blocking
Primary Anitbody
Secondary Antibody
Details
Comments
|
||||||||||||||||||||||||||
reviewed by:
Astrid Spannhoff |
WB | Other | 11/23/2010 |
Summary
Blocking
Primary Anitbody
Secondary Antibody
Details
|
|||||||||||||||||||||||||||
reviewed by:
Dianne Soprano |
WB | Mouse | 09/23/2010 |
Summary
Comments
|
Secondary Antibodies |
Isotype Controls |
Alexa Fluor 350 | NB600-308AF350 | |
Alexa Fluor 405 | NB600-308AF405 | |
Alexa Fluor 488 | NB600-308AF488 | |
Alexa Fluor 532 | NB600-308AF532 | |
Alexa Fluor 594 | NB600-308AF594 | |
Alexa Fluor 647 | NB600-308AF647 | |
Alexa Fluor 700 | NB600-308AF700 | |
Alexa Fluor 750 | NB600-308AF750 | |
Allophycocyanin | NB600-308APC | |
Allophycocyanin/Cy7 | NB600-308APCCY7 | |
Biotin | NB600-308B | |
DyLight 350 | NB600-308UV | |
DyLight 405 | NB600-308V | |
DyLight 488 | NB600-308G | |
DyLight 550 | NB600-308R | |
DyLight 594 | NB600-308DL594 | |
DyLight 650 | NB600-308C | |
DyLight 680 | NB600-308FR | |
DyLight 755 | NB600-308IR | |
FITC | NB600-308F | |
HRP | NB600-308H | |
Janelia Fluor 549 | NB600-308JF549 | |
Janelia Fluor 646 | NB600-308JF646 | |
PE | NB600-308PE | |
PE/Atto594 | NB600-308PEATT594 | |
PE/Cy5.5 | NB600-308PECY55 | |
PE/Cy7 | NB600-308PECY7 | |
PerCP | NB600-308PCP |
Research Areas for GFP Antibody (NB600-308)Find related products by research area.
|
Autophagy and RAS signaling: Clinical implications By Christina Towers, PhD The cellular recycling process known as autophagy is currently being targeted in over 60 clinical trials focused on treating different types of cancer1. To date, the only autophagy-targeted... Read full blog post. |
Neurovascular signaling for repair enhances brain metastasis By Jamshed Arslan, Pharm. D., PhD. Stroke is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t... Read full blog post. |
Make each cell count: How to assess autophagy using flow cytometry Kristy R. Howell, PhDThe cellular recycling process known as autophagy may be induced by a variety of conditions including reduced nutrient availability, serum starvation and pharmacological agents (e.g., Rapamyci... Read full blog post. |
How to visualize autophagy by microscopy By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to... Read full blog post. |
Toll-like receptors in the intestinal epithelial cells By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria... Read full blog post. |
Best way to quantitatively measure Autophagic Flux By Christina Towers, PhD Autophagy is a stress-induced cellular recycling process that plays an important physiological role in many diseases. It is induced by a variety of stimuli, both intracellular and extracel... Read full blog post. |
Animal Models to Study Autophagy By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the... Read full blog post. |
The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura. It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol... Read full blog post. |
GFP - Be Green! Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v... Read full blog post. |
GFP (Green Fluorescent Protein) Bringing Light to Life GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
5 | |
4 | |
3 | |
2 | |
1 |
Anonymous 04/12/2018 |
||
Application: | IHC-Fr | |
Species: | Mouse |
Anonymous 03/22/2017 |
||
Application: | ICC | |
Species: | Mouse |
Jean-Paul Paluzzi 03/10/2017 |
||
Application: | WB | |
Species: | Human |
Uniprot |
|