GFP Antibody

Western Blot: GFP Antibody [NB600-308] - Wild type GFP (0.1 ug) was used to spike HeLa whole cell lysate. Load: 30 ug per lane. Primary antibody: GFP antibody at 1:1000 for overnight at 4C. Secondary antibody: IR Dye800 Goat-anti-Rabbit IgG [H&L] MX10 at 1:10,000 for 45 min at RT. Block: 5% BLOTTO in PBS overnight at 4C. Predicted/Observed size: 27 kDa for epitope tag GFP. Other band(s): none.

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB600-308] - Analysis of GFP in transgenic mouse pancreas in OCT. The blue region in the center is an islet which does not express GFP and is negative. Image from verified customer review.

Western Blot: GFP Antibody [NB600-308] - analysis of GFP tagged PKA subunits over-expressed in HEK293T cells using GFP antibody. Image from verified customer review.

Immunohistochemistry: GFP Antibody [NB600-308] - Analysis of GFP in mouse retinal cells. Image courtesy of anonymous customer review.

Immunohistochemistry-Paraffin: GFP Antibody [NB600-308] - Staining of GFP+ hepatocytes (brown) transplanted into the liver of a recipient animal using anti-GFP antibody. Cyclin D1 double staining in blue. Image from verified customer review.

Immunohistochemistry-Frozen: GFP Antibody [NB600-308] - Adult mouse brain tissue. This image was submitted via customer review.

Immunoprecipitation: GFP Antibody [NB600-308] - IP analysis of GFP in S2 Cells (Drosophila). Image from verified customer review.

• Reactivity: Mu, NA
• Applications: WB, ELISA, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ISH, IHC-FrFl, IHC-WhMt
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.99 mg/ml

GFP Antibody Summary

• Immunogen: This GFP antibody was developed against a fusion protein corresponding to the full length GFP (246aa) derived from the jellyfish Aequorea victoria.
• Specificity: Anti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). Primate reactivity reported in scientific literature (PMID: 27723741).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:5000
  • Electron Microscopy 1:10-1:500
  • ELISA 1:20000-1:120000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:5000
  • Immunohistochemistry 1:200-1:3000
  • Immunohistochemistry-Frozen 1:50-1:250
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • In-situ Hybridization
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
• Application Notes: IF (PMID: 21855203), IP (PMID: 20427314 and PMID: 22298427), ChIP (PMID: 21952049), In-situ Hybridization (PMID: 22340499), Immunohistochemistry-frozen (PMID: 22464327), Electron Microscopy (PMID: 23873149), Immunohistochemistry-whole mount (PMID 24703763) and Immunohistochemistry-free floating reported in scientific literature (PMID 26639405).
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

No reaction was observed against Human, Mouse serum proteins. Suitable for detecting fusion proteins containing the GFP sequence expressed in Human, Mouse, Rat, C. elegans, Drosophila and in vitro transcription/translation systems and transgenic animals. GFP Transgenic Rat reactivity reported in scientific literature (PMID:25724725). Mouse reactivity reported in multiple pieces of scientific literature.Transgenic C. elegans reactivity reported in scientific literature (PMID: 27110099). Known cross reactivity with wt and all variants such as rGFP, eGFP, S65T-GFP, RS-GFP, YFP and EGFP.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 0.99 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GFP Antibody

• anti-gfp (green fluorescent protein) pab
• eGFP
• Enhanced Green Fluorescent Protein
• GFP immunofluorescence
• GFP immunoprecipitation
• GFP mouse
• GFP staining
• GFP western blot
• GFP
• GFPuv
• green fluorescent protein (gfp)
• Green Fluorescent Protein

Background

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T.,... Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S.,... Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB600-308)(157)

Publications using NB600-308

• Thagun C, Chuah J, Numata K Targeted Gene Delivery into Various Plastids Mediated by Clustered Cell Penetrating and Chloroplast Targeting Peptides Adv. Sci. 2019 Oct 23 [PMID: 31832328] (IP, WB, A. thaliana, Plant)
• Mercer Lindsay N, Knutsen PM, Lozada AF et al. Orofacial Movements Involve Parallel Corticobulbar Projections from Motor Cortex to Trigeminal Premotor Nuclei Neuron 2019 Sep 20 [PMID: 31587918]
• Gewin L, Summers ME, Harral JW et al. Inactivation of Tsc2 in Abcg2 Lineage Derived Cells Drives the Appearance of Polycystic Lesions and Fibrosis in the Adult Kidney Am. J. Physiol. Renal Physiol. Aug 28 2019 [PMID: 31461347]
• Zusso M, Lunardi V, Franceschini D et al. Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway J Neuroinflammation Jul 18 2019 [PMID: 31319868] (WB, Mouse)
• Shi C, Pan FC, Kim JN et al. Differential cell susceptibilities to KrasG12D in the setting of obstructive chronic pancreatitis Cell Mol Gastroenterol Hepatol Jul 13 2019 [PMID: 31310834] (IHC, Mouse)
• English JG, Olsen RHJ, Lansu K et al. VEGAS as a Platform for Facile Directed Evolution in Mammalian Cells Cell Jul 25 2019 [PMID: 31280962]
• Nilangekar, K;Murmu, N;Sahu, G;Shravage, BV; Generation and Characterization of Germline-Specific Autophagy and Mitochondrial Reactive Oxygen Species Reporters in Drosophila Front Cell Dev Biol Apr 3 2019 [PMID: 31001531] (WB, Jellyfish)
• Cruz-Ortega, JS;Boucard, AA; Actin cytoskeleton remodeling defines a distinct cellular function for adhesion G protein-coupled receptors ADGRL/latrophilins 1, 2 and 3 Biol Open Apr 23 2019 [PMID: 30926595] (WB, Jellyfish)
• Sandhu R, Wei D, Sharma M, Xu L. An N-terminal Flag-tag impairs TPP1 regulation of telomerase function Biochem. Biophys. Res. Commun. Mar 15 2019 [PMID: 30885434] (WB, Human)
• Li A, Cho JH, Reid B et al. Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks Nat Commun Dec 18 2018 [PMID: 30560870] (IHC, Jellyfish)
• Di Scala C, Tessier M, Sapet C et al. A new polymer-based approach for in vivo transfection in postnatal brain. J Neurosci Methods 2019 Jan 1 [PMID: 30408559]
• Li Q, Saito TT, Martinez-Garcia M et al. The tumor suppressor BRCA1-BARD1 complex localizes to the synaptonemal complex and regulates recombination under meiotic dysfunction in Caenorhabditis elegans. PLoS Genet. 2018 Oct 31 [PMID: 30383767] (WB, Jellyfish)
• Zhao S, Fortier TM, Baehrecke EH. Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr. Biol. Oct 8 2018 [PMID: 30270184] (ICC/IF, Jellyfish)
• Cain NE, Jahed Z, Schoenhofen A et al. Conserved SUN-KASH Interfaces Mediate LINC Complex-Dependent Nuclear Movement and Positioning. Curr. Biol. Sep 8 2018 [PMID: 30245107] (ICC/IF, Jellyfish)
• Spike CA, Huelgas-Morales G, Tsukamoto T, Greenstein D Multiple Mechanisms Inactivate the LIN-41 RNA-Binding Protein To Ensure a Robust Oocyte-to-Embryo Transition in Caenorhabditis elegans Genetics. 2018 Sep 10 [PMID: 30206186] (WB, Jellyfish)
• Wang L, Chen SR, Ma H et al. Regulating nociceptive transmission by VGluT2-expressing spinal dorsal horn neurons. J. Neurochem. Sep 11 2018 [PMID: 30203849] (IHC, Mouse)
• Lee HM, Clark EP, Kuijer MB et al. Characterization and structure-activity relationships of indenoisoquinoline-derived topoisomerase I inhibitors in unsilencing the dormant Ube3a gene associated with Angelman syndrome Mol Autism Aug 17 2018 [PMID: 30140420] (ICC/IF, Mouse)
• Yao H, Price TT, Cantelli G et al. Leukaemia hijacks a neural mechanism to invade the central nervous system. Nature. Jul 18 2018 [PMID: 30022166] (IHC, Jellyfish)
• Nakano Y, kellY MC, Rehman AU et al. Defects in the Alternative Splicing-Dependent Regulation of REST Cause Deafness. Cell. Jul 26 2018 [PMID: 29961578] (ICC/IF, Jellyfish)
• Beer-Hammer S, Lee SC, Mauriac SA et al. Gai Proteins are Indispensable for Hearing. Cell. Physiol. Biochem. Jun 21 2018 [PMID: 29940568] (IHC)
• Stephany CE, Ma X, Dorton HM et al. Distinct Circuits for Recovery of Eye Dominance and Acuity in Murine Amblyopia Curr. Biol. Jun 18 2018 [PMID: 29887305] (Jellyfish)
• Li M, Bian C, Yu X. Poly (ADP-ribosyl) ation is recognized by ECT2 during mitosis. Cell Cycle. 2014 Sep 15 (WB)
• Bell P, Yu H, Kuntz L et al. Preparation of Nonhuman Primate Eyes for Histological Evaluation After Retinal Gene Transfer Hum Gene Ther Methods Jun 1 2018 [PMID: 29848071] (IHC, Jellyfish)
• Du P, Pirouz M, Choi J et al. An Intermediate Pluripotent State Controlled by MicroRNAs Is Required for the Naive-to-Primed Stem Cell Transition Cell Stem Cell May 16 2018 [PMID: 29804889] (IHC)
• Herrera LA, Starr DA The E3 Ubiquitin Ligase MIB-1 Is Necessary To Form the Nuclear Halo in Caenorhabditis elegans Sperm G3 (Bethesda). 2018 May 17 [PMID: 29776970] (IHC, C. elegans)
• Shi B, Xue M, Wang Y et al. An improved method for increasing the efficiency of gene transfection and transduction Int J Physiol Pathophysiol Pharmacol Apr 20 2018 [PMID: 29755642] (ICC/IF, Jellyfish)
• Furuta T, Joo HJ, Trimmer KA et al. GSK-3 promotes S phase entry and progression in C. elegans germline stem cells to maintain tissue output. Development Apr 25 2018 [PMID: 29695611] (Jellyfish)
• Vodicska B, Cerikan B, Schiebel E, Hoffmann I. MISP regulates the IQGAP1/Cdc42 complex to collectively orchestrate spindle orientation and mitotic progression. Sci Rep Apr 20 2018 [PMID: 29679050] (ICC/IF, WB, Jellyfish)
• O'Neill S, Mathis M, Kovacic L et al. Quantitative interaction analysis permits molecular insights into functional NOX4 NADPH oxidase heterodimer assembly. J. Biol. Chem. Apr 19 2018 [PMID: 29674345] (IP, Jellyfish)
• Lebeau P, Platko K, Al-Hashimi AA et al. Loss-of-function PCSK9 mutants evade the unfolded protein response sensor GRP78 and fail to induce endoplasmic reticulum stress when retained. J Biol Chem 2018 May 11 [PMID: 29593095] (Mouse)
• Finn JD, Smith AR, Patel MC et al. A Single Administration of CRISPR/Cas9 Lipid Nanoparticles Achieves Robust and Persistent In Vivo Genome Editing. Cell Rep. Feb 27 2018 [PMID: 29490262] (Jellyfish)
• Dell'Aringa M, Reinhardt RL. Notch signaling represents an important checkpoint between follicular T-helper and canonical T-helper 2 cell fate. Mucosal Immunol. 2018 Feb 21 [PMID: 29467447] (ICC/IF, Jellyfish)
• Wang A, Conicella AE, Schmidt HB, Martin EW. A single N-terminal phosphomimic disrupts TDP-43 polymerization, phase separation, and RNA splicing. EMBO J. 2018 Feb 09 [PMID: 29438978] (WB)
• Pusapati GV, Kong JH, Patel BB, Gouti M. G protein-coupled receptors control the sensitivity of cells to the morphogen Sonic Hedgehog. Sci Signal. 2018 Feb 06 [PMID: 29438014] (Jellyfish)
• Linden AG, Li S, Choi HY et al. Interplay between ChREBP and SREBP-1c coordinates postprandial glycolysis and lipogenesis in livers of mice. J. Lipid Res. Mar 1 2018 [PMID: 29335275] (WB, Mouse)
• Buzgariu W, Wenger Y, Tcaciuc N et al. Impact of cycling cells and cell cycle regulation on Hydra regeneration. Dev Biol. [PMID: 29291976] (ICC/IF, Jellyfish)
• Brumbaugh J, Di Stefano B, Wang X et al. Nudt21 Controls Cell Fate by Connecting Alternative Polyadenylation to Chromatin Signaling. Cell 2017 Nov 30 [PMID: 29249356] (Jellyfish)
• Herring CA, Banerjee A, McKinley ET et al. Unsupervised Trajectory Analysis of Single-Cell RNA-Seq and Imaging Data Reveals Alternative Tuft Cell Origins in the Gut Cell Syst. 2017 Nov 07 [PMID: 29153838] (Mouse)
• Sade N, Umnajkitikorn K, Rubio Wilhelmi MDM et al. Delaying chloroplast turnover increases water-deficit stress tolerance through the enhancement of nitrogen assimilation in rice J Exp Bot. 2018 Feb 05 [PMID: 28992306] (WB, Plant)
  
  Details:
  WB was performed using Oryza sativa lysates.
• Grimaldo L, Sandoval A, Garza-Lopez E, Felix R. Involvement of Parkin in the ubiquitin proteasome system-mediated degradation of N-type voltage-gated Ca2+ channels PLoS ONE 2017 Sep 28 [PMID: 28957379] (IP, WB, Rat)
• Laszczyk AM, Fox-Quick S, Vo HT et al. Klotho regulates postnatal neurogenesis and protects against age-related spatial memory loss Neurobiol. Aging 2017 Jul 29 [PMID: 28837861] (IHC-FrFl, Mouse)
• Tsukamoto T, Gearhart MD, Spike CA et al. LIN-41 and OMA Ribonucleoprotein Complexes Mediate a Translational Repression-to-Activation Switch Controlling Oocyte Meiotic Maturation and the Oocyte-to-Embryo Transition in Caenorhabditis elegans. Genetics. 2017 Jun 01 [PMID: 28576864] (WB, C. elegans)
• Yu D, Maroteaux M, Song Y et al. The transcription factor Shox2 shapes thalamocortical neuron firing properties by regulation of key ion channels bioRxiv (IHC, WB)
• Ogasawara T, Okano S, Ichimura H et al. Impact of extracellular matrix on engraftment and maturation of pluripotent stem cell-derived cardiomyocytes in a rat myocardial infarct model. Sci Rep 2017 Aug 17 [PMID: 28819182] (IHC)
• Bellavance MA, Takatoh J, Lu J et al. Parallel Inhibitory and Excitatory Trigemino-Facial Feedback Circuitry for Reflexive Vibrissa Movement Neuron 2017 Jul 13 [PMID: 28735746]
• Cardoso MA, Fontenele M, Lim B et al. A novel function for the IkB inhibitor Cactus in promoting Dorsal nuclear localization and activity in the Drosophila embryo Development Aug 15 2017 [PMID: 28705899] (ICC/IF, Jellyfish)
• Yoon S, Kawasaki I, Shim YH. cyb-1, a C. elegans B-type cyclin, maintains proper position and number of centrosomes during spermatogenesis J. Cell. Sci. 2017 Jul 13 [PMID: 28705837] (ICC/IF, C. elegans)
• Stauffer BB, Cui G, Cottrill KA et al. Bacterial Sphingomyelinase is a State-Dependent Inhibitor of the Cystic Fibrosis Transmembrane conductance Regulator (CFTR). Sci Rep. 2017 Jun 07 [PMID: 28592822] (ELISA, Xenopus)
• Patra C, Kontarakis Z, Kaur H et al. The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies. Sci Rep. 2017 Jun 02 [PMID: 28578380] (IHC, Zebrafish)
• Knutson AK, Egelhofer T, Rechtsteiner A, Strome S. Germ Granules Prevent Accumulation of Somatic Transcripts in the Adult Caenorhabditis elegans Germline. Genetics. Mar 3 2017 [PMID: 28258184] (ICC/IF, C. elegans)
• Witzel HR, Cheedipudi S, Gao R et al. Isl2b regulates anterior second heart field development in zebrafish Sci Rep Jan 20 2017 [PMID: 28106108] (ICC/IF, Zebrafish)
• Davidson R Pontasch JA Wu JQ. Sbg1 Is a Novel Regulator for the Localization of the b-Glucan Synthase Bgs1 in Fission Yeast. PLoS ONE. Nov 29 2016 [PMID: 27898700] (IP, WB, Yeast)
• Yovchev MI, Oertel M. Fetal Liver Stem/Progenitor Cell Transplantation: A Model to Study Tissue Mass Replacement and Cell-Based Therapies. Methods Mol. Biol. Nov 10 2016 [PMID: 27830548]
• Lee JJ, Rothenberg ME, Seeley ES et al. Control of inflammation by stromal Hedgehog pathway activation restrains colitis. Proc. Natl. Acad. Sci. U.S.A. Nov 22 2016 [PMID: 27815529] (Mouse)
• Bao K, Carr T, Wu J et al. BATF Modulates the Th2 Locus Control Region and Regulates CD4+ T Cell Fate during Antihelminth Immunity. J. Immunol. Oct 26 2016 [PMID: 27798167] (IHC, Jellyfish)
• Shiba Y, Gomibuchi T, Seto T et al. Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts. Nature Oct 10 2016 [PMID: 27723741] (IHC-P, Primate)
• Ahn JH, Rechsteiner A, Strome S, Kelly WG. A Conserved Nuclear Cyclophilin Is Required for Both RNA Polymerase II Elongation and Co-transcriptional Splicing in Caenorhabditis elegans. PLoS Genet. Aug 1 2016 [PMID: 27541139] (ICC/IF, IP)
• Liu Y, Lee IJ, Sun M et al. Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis Mol. Biol. Cell 2016 Jul 06 [PMID: 27385337] (IP, Jellyfish)
• Barrionuevo FJ, Hurtado A, Kim GJ et al. Sox9 and Sox8 protect the adult testis from male-to-female genetic reprogramming and complete degeneration. Elife Jun 21 2016 [PMID: 27328324] (IHC, Mouse)
• Zeng Y, Guo W, Xu G et al. Xyloketal-derived small molecules show protective effect by decreasing mutant Huntingtin protein aggregates in Caenorhabditis elegans model of Huntington's disease. Drug Des Devel Ther. 2016 Apr 25 [PMID: 27110099] (WB, C. elegans)
• Myeku N, Clelland CL, Emrani S et al. Tau-driven 26S proteasome impairment and cognitive dysfunction can be prevented early in disease by activating cAMP-PKA signaling. Nat. Med. 2016 Jan 01 [PMID: 26692334] (WB)
• Sorrentino NC, Maffia V, Strollo S et al. A Comprehensive Map of CNS Transduction by Eight Recombinant Adeno-associated Virus Serotypes Upon Cerebrospinal Fluid Administration in Pigs. Mol. Ther. 2015 Dec 07 [PMID: 26639405] (WB, IHC-FrFl, IHC-Fr)
• Gross S, Balderes D, Liu J et al. Nkx2.2 is expressed in a subset of enteroendocrine cells with expanded lineage potential. Am J Physiol Gastrointest Liver Physiol 2015 Dec 15 [PMID: 26492922]
• Rueckemann JW, DiMauro AJ, Rangel LM et al. Transient optogenetic inactivation of the medial entorhinal cortex biases the active population of hippocampal neurons. Hippocampus 2015 Aug 24 [PMID: 26299904] (IHC-Fr, Rat)
• Akhtar Ali M, Younis S, Wallerman O et al. Transcriptional modulator ZBED6 affects cell cycle and growth of human colorectal cancer cells. Proc Natl Acad Sci U S A 2015 Jun 23 [PMID: 26056301] (WB, Human)
• Gabrusiewicz K, Hossain MB, Cortes-Santiago N et al. Macrophage Ablation Reduces M2-Like Populations and Jeopardizes Tumor Growth in a MAFIA-Based Glioma Model Neoplasia 2015 Apr 01 [PMID: 25925380] (ICC/IF, IHC-P, Mouse)
• Gautam M, Fujita D, Kimura K et al. Transplantation of adipose tissue-derived stem cells improves cardiac contractile function and electrical stability in a rat myocardial infarction model J. Mol. Cell. Cardiol. 2015 Feb 24 [PMID: 25724725] (IHC-P, Rat)
• Doi K, Ogata F, Eto H et al. Differential contributions of graft- and host-derived cells in tissue regeneration/remodeling after fat grafting Plast. Reconstr. Surg. 2015 Feb 20 [PMID: 25724061] (IHC-P)
• Matthews DW, Deschenes M, Furuta T et al. Feedback in the brainstem: An excitatory disynaptic pathway for control of whisking. J. Comp. Neurol. 2014 Dec 12 [PMID: 25503925] (IHC, Mouse)
• Li M, Bian C, Yu X. Poly(ADP-ribosyl)ation is recognized by ECT2 during mitosis. Cell Cycle 2014 [PMID: 25486481] (WB)
• Davidson R, Laporte D, Wu JQ. Regulation of Rho GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis. Mol. Biol. Cell. 2014 Dec 03 [PMID: 25473118] (IP)
  
  Details:
  GFP antibody used in IP for pulling down the YFP-tagged protein expressed at its native level from fission yeast cell extracts (Figure 3E)
• Meng L, Ward AJ, Chun S et al. Towards a therapy for Angelman syndrome by targeting a long non-coding RNA. Nature. 2014 Dec 01 [PMID: 25470045] (WB)
• Liu D, Yovchev Mi, Zhang J et al. Identification and Characterization of Mesenchymal-Epithelial Progenitor-Like Cells in normal and Injured Rat Liver. Am. J. Pathol. 2014 Nov 06 [PMID: 25447047]
• Yap My, Lo Yl, Talbot K, Ong Wy. Oxidative stress reduces levels of dysbindin-1A via its PEST domain. Neurochem. Int. 2014 Oct 31 [PMID: 25445987] (WB, Human)
• Nelson C, Ambros V, Baehrecke Eh. miR-14 Regulates Autophagy during Developmental Cell Death by Targeting ip3-kinase 2. Mol. Cell. 2014 Oct 08 [PMID: 25306920] (WB)
• Bratton B, Wright Cw. NF-kB-inducing kinase (nIK) contains an amino terminal inhibitor of apoptosis (IAP) binding motif (IBM) that potentiates nIK degradation by c-IAP1* J. Biol. Chem. et al. 2014 Sep 22 [PMID: 25246529] (WB)
• Cain NE, Tapley EC, McDonald KL et al. The SUN protein UNC-84 is required only in force-bearing cells to maintain nuclear envelope architecture. J. Cell Biol. 2014 Jul 21 [PMID: 25023515] (IHC-WhMt)
  
  Details:
  GFP antibody used for immunofluorescence staining of C elegans embryos - antibody diluted 1:200 in UNC-83 antiserum for simultaneous staining of KASH and SUN chimeric proteins.
• Shigunov Patricia, Sotelo-Silveira Jose, Stimamiglio Marco Augusto et al. Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules. BMC Mol Biol. 2014 Jul 03 [PMID: 24993635] (IP)
• Goetsch PD, Strome S DREAM Interrupted: Severing MuvB from DREAM's pocket protein impairs gene repression but not DREAM assembly on chromatin BioRxiv 2019 Jun 13
• Moon H, Zhu J, Donahue LR et al. Electron transport chain biogenesis activated by a JNK-insulin-Myc relay primes mitochondrial inheritance in Drosophila bioRxiv May 18 2019 (IHC, Drosophila)
• Autry AE, Wu Z, Kohl J, Bambah-Mukku D Perifornical Area Urocortin-3 Neurons Promote Infant-directed Neglect and Aggression bioRxiv Jul 9 2019 (IHC, Mouse)
• Wang N, Lo Presti L, Zhu YH et al. The novel proteins Rng8 and Rng9 regulate the myosin-V Myo51 during fission yeast cytokinesis. J. Cell Biol. 2014 May 12 [PMID: 24798735] (IP)
• Dufeys C Exploring the role of fibroblastic AMPKalpha1 in ventricular remodelling after myocardial infarction: implication of connexin 43 and miR-125b Thesis 2019
• Buzgariu W, Crescenzi M, Galliot B. Robust G2 pausing of adult stem cells in Hydra. Differentiation 2014 Apr 21 [PMID: 24703763] (ICC/IF, IHC-WhMt)
• Walter TJ, Vanderpool C, Cast AE, Huppert SS. Intrahepatic Bile Duct Regeneration in Mice Does Not Require Hnf6 or Notch Signaling through Rbpj. Am. J. Pathol. 3/13/2014 [PMID: 24631193] (IHC-P)
• Klos KS, Warmka JK, Drachenberg DM et al. Building Bridges toward Invasion: Tumor Promoter Treatment Induces a Novel Protein Kinase C-Dependent Phenotype in MCF10A Mammary Cell Acini. PLoS ONE 3/6/2014 [PMID: 24599099] (ICC/IF)
• Montgomery Scott R, Nargizyan Taya, Meliton Vicente et al. A novel osteogenic oxysterol compound for therapeutic development to promote bone growth: activation of hedgehog signaling and osteogenesis through smoothened binding. J Bone Miner Res. 2014 Aug 01 [PMID: 24591126]
• Hagiwara M, Kokubu E, Sugiura S et al. Vinculin and Rab5 Complex Is Requited for Uptake of Staphyrococcus aureus and Interleukin-6 Expression. PLoS ONE 2014 Jan 27 [PMID: 24466349] (WB, IP)
• Tian G, Ropelewski P, Nemet I et al. An unconventional secretory pathway mediates the Cilia targeting of peripherin/rds. J. Neurosci. 2014 Jan 15 [PMID: 24431457] (WB)
• Nachtergaele S, Whalen DM, Mydock LK et al. Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling. Elife. 2013 Oct 29 [PMID: 24171105] (WB)
• Oldenbroek M, Robertson SM, Guven-Ozkan T et al. Regulation of maternal Wnt mRNA translation in C. elegans embryos. Development. 2013 Nov [PMID: 24131629] (IP)
• Hullinger R, Ugalde J, Puron-Sierra L et al. The MRI contrast agent gadoteridol enhances distribution of rAAV1 in the rat hippocampus. Gene Ther. 2013 Sep 19 [PMID: 24048419] (IHC-Fr)
• Zhu YH, Ye Y, Wu Z, Wu JQ. Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis. Mol Biol Cell. 2013 Oct [PMID: 23966468] (IP, Yeast)
• Park BH, Kook S, Lee S et al. An isoform of C/EBPbeta, LIP, regulates expression of the chemokine receptor CXCR4 and modulates breast cancer cell migration. J Biol Chem. 2013 Oct 4 [PMID: 23966000] (IHC-P)
• Chang TK, Shravage BV, Hayes SD et al. Uba1 functions in Atg7- and Atg3-independent autophagy. Nat Cell Biol 2013 Jul 21 [PMID: 23873149] (EM, Firefly)
• Huang WC, Yang CC, Chen IH et al. Treatment of Glucocorticoids Inhibited Early Immune Responses and Impaired Cardiac Repair in Adult Zebrafish. PLoS One 2013 Jun 21 [PMID: 23805247] (IHC-Fr, ICC/IF, Zebrafish)
• Wang G, Li Y, Wang XY et al. Slit/Robo1 signaling regulates neural tube development by balancing neuroepithelial cell proliferation and differentiation. Exp Cell Res 2013 Feb 21 [PMID: 23438940] (IHC, ICC/IF, Firefly)
• Yang JR, Hsu CW, Liao SC et al. Transplantation of embryonic stem cells improves the regeneration of periodontal furcation defects in a porcine model. J Clin Periodontol 2013 Apr [PMID: 23431981] (IHC-P, Firefly)
• Shravage BV, Hill JH, Powers CM et al. Atg6 is required for multiple vesicle trafficking pathways and hematopoiesis in Drosophila. Development 2013 Mar [PMID: 23406899] (IHC-P, Firefly)
• Natalia Vergara M, Gutierrez C, O'Brien DR et al. Ex vivo electroporation of retinal cells: A novel, high efficiency method for functional studies in primary retinal cultures. Exp Eye Res 2013 Jan 28 [PMID: 23370269] (ICC/IF, Chicken)
• Shinojima N, Hossain A, Takezaki T et al. TGF-beta Mediates Homing of Bone Marrow-Derived Human Mesenchymal Stem Cells to Glioma Stem Cells Cancer Res 2013 Apr 1 [PMID: 23365134] (ICC/IF, Firefly)
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Reviews for GFP Antibody (NB600-308) (18)

Average Rating: 4.5

(Based on 18 reviews)

We have 18 reviews tested in 4 species: Human, Mouse, Rat, Other.

reviewed by: Anonymous IHC-Fr Mouse 04/12/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Adult mouse brain tissue
• Species: Mouse
• Lot: 35459

Comments

• Comments: Dilutions 1:400

reviewed by: Anonymous ICC Mouse 03/22/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: FFPE
• Species: Mouse

Comments

• Comments: Trypsin retrieval

reviewed by: Jean-Paul Paluzzi WB Human 03/10/2017

Summary

• Application: Western Blot
• Sample Tested: whole cellular protein and fractionated (soluble and membrane protein)
• Species: Human

Comments

• Comments: Human embryonic kidney 293T
  HEK293T cells

reviewed by: Wenchao Zhou Human 03/02/2017

Summary

• Sample Tested: Human Xenograft Tumor
• Species: Human

reviewed by: Anonymous IF Other 07/27/2016

Summary

• Application: Immunofluorescence
• Sample Tested: Pig TM cells
• Species: Other

Blocking

• Blocking Details: -

Details

• Detection Notes: -

reviewed by: Anonymous IF Mouse 10/10/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse, 3T3 cells
• Species: Mouse
• Lot: 28839

Blocking

• Blocking Details: 1% donkey serum+1% BSA+0.1% Triton X-100 in 1xPBS (Blocking Buffer)

Primary Anitbody

• Dilution Ratio: 1:500, 1 hr at room temperature. Primary antibody was diluted in blocking buffer

Secondary Antibody

• Secondary Description: Alexa Fluor 488 conjugated to Donkey anti-rabbit IgG
• Secondary Manufacturer Cat#: A-21206
• Secondary Concentration: 1:500

Details

• Detection Notes: Images were obtained on a Leica SP8 confocal microscope with 63X objective and a 6x zoom factor
• Fixation Details: Methanol fixation for 15 min at -20 deg
• Wash Description: 1x PBS wash, 5 min each wash, 3 washes

reviewed by: Anonymous WB Human 10/10/2015

Summary

• Application: Western Blot
• Sample Tested: HEK293T whole cell extract
• Species: Human
• Lot: 28839

Blocking

• Blocking Details: 5% milk in TBS-T (0.1% Tween)

Primary Anitbody

• Dilution Ratio: 1:5000, overnight at 4 deg, primary antibody was diluted in TBS-T (0.1% Tween) with 5% milk

Secondary Antibody

• Secondary Description: Peroxidase conjugated Donkey anti-Rabbit IgG (H+L)
• Secondary Manufacturer Cat#: 711-035-152
• Secondary Concentration: 0.04 µg/ml

Details

• Detection Notes: ECL, Film, 5 sec, GFP tagged constructs

Comments

• Comments: This antibody works very well in Western Blotting

reviewed by: Anonymous IP Other 04/02/2015

Summary

• Application: Immunoprecipitation
• Sample Tested: S2 Cells (Drosophila)
• Species: Other

Blocking

• Blocking Details: BSA 1%, Incubation time: 3h at 4°C

Primary Anitbody

• Dilution Ratio: polyclonal anti-GFP antibody, 1:1000; Incubation time: 3h at 4°C

Details

• Detection Notes: Detection was perfomed in Wersten Blot by using polyclonal anti-GFP antibody, 1:1000. An load control was used as control.
• Immunoprecipitation Details: protein-A Sepharose beads; Incubation time: 3h at 4°C; Beads were washed 3 times with lysis buffer

reviewed by: Megan Cleveland IF Mouse 11/25/2014

Summary

• Application: Immunofluorescence
• Sample Tested: transgenic mouse pancreas
• Species: Mouse

Blocking

• Blocking Details: block 1 hr at RT with 10% FBS PBST

Primary Anitbody

• Dilution Ratio: 1/100 in 10% FBS PBST, incubated overnight at 4 C

Secondary Antibody

• Secondary Description: Goat anti-rabbit Cy3 conjugated
• Secondary Manufacturer Cat#: Jackson Immunoresearch Laboratories 111-166-045
• Secondary Concentration: 1/200

Details

• Detection Notes: Zeiss fluorescent microscope 8s; this transgenic mouse was the positive control; neg con is secondary only/negative islet tissue
• Fixation Details: 4% PFA for 4 hours, cryopreserve in 30% sucrose overnight, permeabilize with PBST 15 min at RT
• Wash Description: wash 3x in PBST, 10 min each

Comments

• Comments: the blue region in the center is an islet which does not express GFP and is negative, as expected

reviewed by: Anonymous IHC-P 10/05/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Lot: 28839
• Comments: Excellent product, works well on Paraffin embedded and PFA/Sucrose fixed sections. Highly recommended.

reviewed by: Samantha Garcia IHC-Fr Mouse 11/06/2013

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Transgenic mouse pancreas in OCT
• Species: Mouse
• Lot: 24437
• Comments: The staining with the antibody at 1:100 was very bright. I could have increased the dilution to 1:300.

Blocking

• Blocking Details: 10% FBS in PBST

Primary Anitbody

• Dilution Ratio: 1:100 in blocking buffer

Comments

• Comments: The staining with the antibody at 1:100 was very bright. I could have increased the dilution to 1:300.

reviewed by: Mladen Yovchev IF Rat 07/13/2012

Summary

• Application: Immunofluorescence
• Sample Tested: Rat liver
• Species: Rat
• Lot: 24437
• Comments: This is the only antibody I found on the market that detects EGFP. It is very strong and specific. I have no issues with background. I have used at least 5 vials and there are some lot to lot differences but overall the performance is the same.

Blocking

• Blocking Details: blocking 1h in PBS/1% donkey serum/1% BSA

Primary Anitbody

• Dilution Ratio: 1:400 dilution, incubation for 2h at RT in blocking buffer

Secondary Antibody

• Secondary Description: anti-rabbit IgG DyLight488
• Secondary Manufacturer Cat#: Vector DI-1488
• Secondary Concentration: Dilution 1:100

Details

• Fixation Details: 4% PFA for 10 min than 0.3% Triton X100 in PBS for 10 min on ice
• Wash Description: PBS/0.05% Tween20, 2x 10 min each

Comments

• Comments: This is the only antibody I found on the market that detects EGFP. It is very strong and specific. I have no issues with background. I have used at least 5 vials and there are some lot to lot differences but overall the performance is the same.

reviewed by: Marisa Carbonaro IP Human 04/24/2012

Summary

• Application: Immunoprecipitation
• Sample Tested: Hela whole cell lysate
• Species: Human

reviewed by: Shao-Win Wang WB Other 11/15/2011

Summary

• Application: Western Blot
• Sample Tested: Yeast
• Species: Other

Blocking

• Blocking Details: Blocking Buffer: 5% Milk in BSA

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:2000, Primary Ab Incubation Time: 10 minutes

Secondary Antibody

• Secondary Description: Secondary Ab: anti-rabbit, Secondary Ab Dilution Ratio: 1:5000

Details

• Detection Notes: Detection Method: ECL

reviewed by: Anonymous IHC Mouse 09/28/2011

Summary

• Application: Immunohistochemistry
• Sample Tested: IHC on parrafin sections (mouse)
• Species: Mouse
• Comments: This is the only GFP antibody on the market that we could find for paraffin sections. It requires special retrieval conditions: 100mm tris pH10, in pressure with 15 minutes of high pressure and two hours of total heat.
• Pub Med ID: 21311774

Comments

• Comments: This is the only GFP antibody on the market that we could find for paraffin sections. It requires special retrieval conditions: 100mm tris pH10, in pressure with 15 minutes of high pressure and two hours of total heat.

reviewed by: Huang Sheng Chieh IF Mouse 07/20/2011

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse Retinal Cells
• Species: Mouse
• Comments: Transgenic mice have FITC expresion in retina inner nuclear layer.

Blocking

• Blocking Details: Blocking Buffer: 5% goat serum, Blocking Time: overnight, Blocking Temp: 4 degrees Celsius

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:200

Secondary Antibody

• Secondary Description: Secondary Ab: Alexa Fluor 488 goat anti-rabbit IgG (H&L)

Details

• Detection Notes: Detection Visualization Method: Leica TCS SP5
• Fixation Details: Sample Preparation: 4% PFA treated slides

Comments

• Comments: Transgenic mice have FITC expresion in retina inner nuclear layer.

reviewed by: Astrid Spannhoff WB Other 11/23/2010

Summary

• Application: Western Blot
• Sample Tested: Pig ear fibroblasts, Sample Amount: 10 ul
• Species: Other

Blocking

• Blocking Details: Blocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: 20 degrees Celsius

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:500, Incubation Dilution Buffer: 5% Milk in PBST, Incubation Time: 1hr, Incubation Temp: 20C

Secondary Antibody

• Secondary Description: Secondary Ab: anti-rabbit IgG, Secondary Ab Dilution Ratio: 1:5000
• Secondary Manufacturer Cat#: NA9340

Details

• Detection Notes: Detection Method: ECL, Exposure Time: 1 minute, Specific Bands: 27 kDa

reviewed by: Dianne Soprano WB Mouse 09/23/2010

Summary

• Application: Western Blot
• Sample Tested: P19 cells transfectedwith a plasmid DNA expressing a GFP fusion protein, Sample Amount: 20 micrograms
• Species: Mouse
• Comments: The GFP antibody worked just fine. We obtained strong bands with little to no background. We used standard procedure as recommended by LiCor for using with the Odyssey Instrument for detection.

Comments

• Comments: The GFP antibody worked just fine. We obtained strong bands with little to no background. We used standard procedure as recommended by LiCor for using with the Odyssey Instrument for detection.

FAQs for GFP Antibody (NB600-308). (Showing 1 - 1 of 1 FAQs).

1. Does the GFP antibody NB600-308 also work against AcGFP (93% identical to EGFP) in IP and Western blot?
   • For our product NB600-308, we have unfortunately not yet tested the product for cross reactivity with AcGFP. However, since the antibody is polyclonal and made to the full length AvGFP sequence, there is a good chance for cross reactivity.

Bioinformatics

• Uniprot:
  • P42212()