GFP Antibody (4B10B2)

Images

 
Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Western blot analysis using GFP mouse mAb against extracts from HCC827 cells, untransfected (1) and transfected with GFP(2).
Immunocytochemistry/ Immunofluorescence: GFP Antibody (4B10B2) [NBP2-22111] - Immunocytochemistry analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP mouse mAb.
Flow Cytometry: GFP Antibody (4B10B2) [NBP2-22111] - Flow cytometric analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP mouse mAb.
Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Western blot analysis using GFP mouse mAb against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).

Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-P
Clone
4B10B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

GFP Antibody (4B10B2) Summary

Immunogen
Purified recombinant fragment of GFP expressed in E. coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This GFP (4B10B2) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry and ELISA.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with GFP.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for GFP Antibody (4B10B2)

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFP Antibody (NBP2-22111). (Showing 1 - of FAQ).

Secondary Antibodies

 

Isotype Controls

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Research Areas for GFP Antibody (NBP2-22111)

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Blogs on GFP.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

GFP - Be Green!
Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

GFP (Green Fluorescent Protein) Bringing Light to Life
GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

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