GFP Antibody [Unconjugated]

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Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB, Simple Western, IP, CyTOF-ready, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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GFP Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant GFPuv
Ser2-Lys238
Accession # P42212
Specificity
Detects GFP in direct ELISAs and Western blots.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GFP
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 2.5 ug/mL
  • Immunoprecipitation 25 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 1-15 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
Reviewed Applications
Read 1 Review rated 5
using
AF4240 in the following applications:

Publications
Read Publications using
AF4240 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFP Antibody [Unconjugated]

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

Green fluorescent protein (GFP) is a 27 kDa protein originally isolated from the jellyfish Aequorea victoria. In the presence of UV light (490-520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in length. It is a globular monomer that has a tendency to dimerize. The monomer has the shape of a beta -barrel with a chromophore (aa 65-67) containing alpha -helix running up its center. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. This form expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395 nm and emission at 508 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (AF4240)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC, Immunoprecipitation.


Filter By Application
IHC
(1)
Immunoprecipitation
(1)
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Filter By Species
Mouse
(1)
All Species

Review for GFP Antibody (AF4240) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using AF4240:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous
WB Other 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 20484048
SpeciesOther

Blocking

Blocking DetailsSee PMID 20484048

Details

Detection NotesSee PMID 20484048

Comments

CommentsPublished: See PMID 20484048Independent review from antibodyresource.com

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GFP Antibody (AF4240). (Showing 1 - of FAQs).

    Secondary Antibodies

     

    Isotype Controls

    Other Available Formats

    Allophycocyanin IC4240A
    Phycoerythrin IC4240P

    Additional GFP Products

    Bioinformatics Tool for GFP Antibody (AF4240)

    Discover related pathways, diseases and genes to GFP Antibody (AF4240). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
    Visit Tool

    Blogs on GFP. Showing 1-10 of 12 blog posts - Show all blog posts.

    Autophagy and RAS signaling: Clinical implications
    By Christina Towers, PhD The cellular recycling process known as autophagy is currently being targeted in over 60 clinical trials focused on treating different types of cancer1. To date, the only autophagy-targeted...  Read full blog post.

    Neurovascular signaling for repair enhances brain metastasis
    By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.

    Make each cell count: How to assess autophagy using flow cytometry
    Kristy R. Howell, PhDThe cellular recycling process known as autophagy may be induced by a variety of conditions including reduced nutrient availability, serum starvation and pharmacological agents (e.g., Rapamyci...  Read full blog post.

    How to visualize autophagy by microscopy
    By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to...  Read full blog post.

    Toll-like receptors in the intestinal epithelial cells
    By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

    Best way to quantitatively measure Autophagic Flux
    By Christina Towers, PhD Autophagy is a stress-induced cellular recycling process that plays an important physiological role in many diseases. It is induced by a variety of stimuli, both intracellular and extracel...  Read full blog post.

    Animal Models to Study Autophagy
    By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.

    The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
    GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

    GFP - Be Green!
    Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

    GFP (Green Fluorescent Protein) Bringing Light to Life
    GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

    Showing 1-10 of 12 blog posts - Show all blog posts.

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    Recent Reviews

    5
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    1
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    1
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    Anonymous
    01/08/2015
    Application: WB
    Species: Other

    Bioinformatics

    Gene Symbol GFP
    Uniprot