GFP Antibody [Allophycocyanin]

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HEK293 human embryonic kidney cell line transfected with (A) wild-type GFP or (B) eGFP was stained with Goat Anti-GFP APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC4240A). Quadrant markers ...read more

Product Details

Summary
Reactivity MultiSpecies Glossary
Applications Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Allophycocyanin

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Catalog# & Conjugate Size Price
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GFP Antibody [Allophycocyanin] Summary

Immunogen
E. coli-derived recombinant GFPuv
Ser2-Lys238
Accession # P42212
Specificity
Detects GFP in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GFP
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publication using
IC4240A in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFP Antibody [Allophycocyanin]

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

Green Fluorescent Protein (GFP) is a 27 kDa protein originally isolated from the jellyfish Aequorea victoria. In the presence of UV light (490-520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in length. It is a globular monomer that has a tendency to dimerize. The monomer has the shape of a beta -barrel with a chromophore (aa 65-67) containing alpha -helix running up its center. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. This form expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395 nm and emission at 508 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (IC4240A)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow.


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FAQs for GFP Antibody (IC4240A). (Showing 1 - of FAQ).

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    Bioinformatics Tool for GFP Antibody (IC4240A)

    Discover related pathways, diseases and genes to GFP Antibody (IC4240A). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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    Blogs on GFP.

    The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
    GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

    GFP - Be Green!
    Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

    GFP (Green Fluorescent Protein) Bringing Light to Life
    GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

    BDNF Antibodies Aid Research on Alzheimer's Therapies
    Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

    Epitope Tags and the V5 Tag Antibody
    We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

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    Bioinformatics

    Gene Symbol GFP
    Uniprot