GFP Antibody

Images

 
Western Blot: eGFP Antibody [NBP2-37821] - WB analysis on HEK293 cells transfected with Green Fluorescent Protein (GFP) using eGFP antibody. 20ug whole cells lyate were loaded. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: eGFP Antibody [NBP2-37821] - Analysis of eGFP using either natural fluorescence (green) or an eGFP antibody (red) in U2OS cells transfected with an EGFR-eGFP fusion protein.
Western Blot: eGFP Antibody [NBP2-37821] - 12% SDS-PAGE of HEK-293 cells transfected with pCMV-Myc-eGFP (0.25ug DNA lanes 2 and 4; 0.50ug DNA lanes 3 and 5) Coomassie stain (lanes 2 and 3) and western blot (lanes 4 and ...read more

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GFP Antibody Summary

Immunogen
This GFP antibody was developed against purified recombinant eGFP
Specificity
GFP antibody detects eGFP in samples. This product has been successfully used in Western Blot and ICC/IF applications. The immunogen is purified recombinant eGFP.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:20 - 1:200
Reviewed Applications
Read 1 Review rated 5
using
NBP2-37821 in the following applications:

Publications
Read Publication using
NBP2-37821 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS (pH 7.2) with 0.1% gelatin
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GFP Antibody

  • anti-gfp (green fluorescent protein) pab
  • clone 9F9.F9
  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP immunofluorescence
  • GFP immunoprecipitation
  • GFP monoclonal
  • GFP mouse
  • GFP staining
  • GFP western blot
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T.,... Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S.,... Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NBP2-37821)(1)

We have publications tested in 1 confirmed species: Jellyfish.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for GFP Antibody (NBP2-37821) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Non-species related.

Reviews using NBP2-37821:
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WB
(1)
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Filter by Species
Non-species related
(1)
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Images Ratings Applications Species Date Details
Western Blot GFP NBP2-37821
Enlarge
5
reviewed by:
Anonymous
WB Non-species related 02/02/2017
View

Summary

ApplicationWestern Blot
Sample TestedTransfected HEK 293 cells
SpeciesNon-species related
LotRA225458

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFP Antibody (NBP2-37821). (Showing 1 - of FAQ).

    Secondary Antibodies

     

    Isotype Controls

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    Additional GFP Products

    Research Areas for GFP Antibody (NBP2-37821)

    Find related products by research area.

    Blogs on GFP. Showing 1-10 of 12 blog posts - Show all blog posts.

    Autophagy and RAS signaling: Clinical implications
    By Christina Towers, PhD The cellular recycling process known as autophagy is currently being targeted in over 60 clinical trials focused on treating different types of cancer1. To date, the only autophagy-targeted...  Read full blog post.

    Neurovascular signaling for repair enhances brain metastasis
    By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.

    Make each cell count: How to assess autophagy using flow cytometry
    Kristy R. Howell, PhDThe cellular recycling process known as autophagy may be induced by a variety of conditions including reduced nutrient availability, serum starvation and pharmacological agents (e.g., Rapamyci...  Read full blog post.

    How to visualize autophagy by microscopy
    By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to...  Read full blog post.

    Toll-like receptors in the intestinal epithelial cells
    By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

    Best way to quantitatively measure Autophagic Flux
    By Christina Towers, PhD Autophagy is a stress-induced cellular recycling process that plays an important physiological role in many diseases. It is induced by a variety of stimuli, both intracellular and extracel...  Read full blog post.

    Animal Models to Study Autophagy
    By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.

    The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
    GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

    GFP - Be Green!
    Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

    GFP (Green Fluorescent Protein) Bringing Light to Life
    GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

    Showing 1-10 of 12 blog posts - Show all blog posts.

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    Recent Reviews

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    Anonymous
    02/02/2017
    Application: WB
    Species: Non-species related