GFP Antibody [FITC]

Images

 
Western Blot: GFP Antibody [FITC] [NB100-1771] - Lane 1: HeLa cells. Lane 2: mock transfected HeLa cell lysate. Load: 35 ug per lane. Primary antibody: GFP antibody at 1 ug/ml for 1 h at room temperature. Secondary ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [FITC] [NB100-1771] - Tissue: Drosophila melanogaster late stage embryonic central nervous system. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary ...read more
Western Blot: GFP Antibody [FITC] [NB100-1771] - Lane 1: GFP. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block: ...read more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [FITC] [NB100-1771] - Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, ...read more

Product Details

Summary
Reactivity JfSpecies Glossary
Applications WB, FLISA, Flow, ICC/IF, IHC-Fr, PLA
Clonality
Polyclonal
Host
Goat
Conjugate
FITC

Order Details

GFP Antibody [FITC] Summary

Immunogen
Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish (Aequorea victoria).
Specificity
GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GFP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Flow Cytometry 1:10-1:1000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:500-1:2500
  • Immunohistochemistry-Frozen
  • Proximity Ligation Assay
Publications
Read Publications using
NB100-1771 in the following applications:

Reactivity Notes

No reaction was observed against Human, or Rat serum proteins. Known Cross Reactivity: rGFP. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants. Reactivity in transgenic mice with GFP. Reactivity in human cell lines transfected will a GFP construct. Mouse reactivity reported in multiple pieces of scientific literature.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for GFP Antibody [FITC]

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFP
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein

Background

Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation. Fluorescein conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB100-1771)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: FLOW, ICC/IF, IHC-Fr, PLA.


Filter By Application
FLOW
(1)
ICC/IF
(2)
IHC-Fr
(3)
PLA
(1)
All Applications
Filter By Species
Mouse
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-1771 Applications Species
Doerner JF, Delling M, Clapham DE. Ion channels and calcium signaling in motile cilia. Elife. 2015 Dec 09 [PMID: 26650848] (IHC-Fr, Mouse) IHC-Fr Mouse
Ntokou A, Klein F, Dontireddy D et al. Characterization of the platelet-derived growth factor receptor a-positive cell lineage during murine late lung development. Am. J. Physiol. Lung Cell Mol. Physiol. 2015 Aug 28 [PMID: 26320158] (IHC-Fr) IHC-Fr
Hauser J, Grundstrom C, Grundstrom T. Allelic Exclusion of IgH through Inhibition of E2A in a VDJ Recombination Complex. J. Immunol. 2014 Feb 24 [PMID: 24470503] (PLA) PLA
Freeman K, Tao W, Sun H et al. In situ three-dimensional reconstruction of mouse heart sympathetic innervation by two-photon excitation fluorescence imaging. J Neurosci Methods. 2013 Sep 19 [PMID: 24056230] (IHC-Fr, ICC/IF) IHC-Fr, ICC/IF
Lampron A, Pimentel-Coelho PM, Rivest S. Migration of bone marrow-derived cells into the CNS in models of neurodegeneration. J Comp Neurol 2013 May 16 [PMID: 23682015] (FLOW) FLOW
Hauser J, Sveshnikova N, Wallenius A, Baradaran S, Saarikettu J, Grundstrom T. B-cell receptor activation inhibits AID expression through calmodulin inhibition of E-proteins. PNAS, 2008;105(4):1267-72. 29-Jan. [PMID: 18203819]
Yee, Min et al. Type II epithelial cells are critical target for hyperoxia-mediated impairment of postnatal lung development. Am J Physiol Lung Cell Mol Physiol 291: L1101-L1111. 2006 [PMID: 16861382]
Dontireddy DA. Identification of pulmonary PDGFRalpha-positive fibroblast specific miRNA and mRNA expression profiles during postnatal lung development Thesis 2015 (ICC/IF, Mouse) ICC/IF Mouse

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFP Antibody (NB100-1771). (Showing 1 - of FAQs).

Secondary Antibodies

 

Isotype Controls

Additional GFP Products

Bioinformatics Tool for GFP Antibody (NB100-1771)

Discover related pathways, diseases and genes to GFP Antibody (NB100-1771). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on GFP.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

GFP - Be Green!
Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

GFP (Green Fluorescent Protein) Bringing Light to Life
GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

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Bioinformatics

Gene Symbol GFP