GFP Antibody [FITC]


Western Blot: GFP Antibody [FITC] [NB100-1771] - Lane 1: HeLa cells. Lane 2: mock transfected HeLa cell lysate. Load: 35 ug per lane. Primary antibody: GFP antibody at 1 ug/ml for 1 h at room temperature. Secondary more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [FITC] [NB100-1771] - Tissue: Drosophila melanogaster late stage embryonic central nervous system. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary more
Western Blot: GFP Antibody [FITC] [NB100-1771] - Lane 1: GFP. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block: more
Immunocytochemistry/ Immunofluorescence: GFP Antibody [FITC] [NB100-1771] - Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, more

Product Details

Reactivity InSpecies Glossary
Applications WB, FLISA, Flow, ICC/IF, IHC-Fr, PLA
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

GFP Antibody [FITC] Summary

Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish (Aequorea victoria).
GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
FITC (A=495, E=519)
0.02 M Potassium Phosphate (pH 7.2), and 0.15 M NaCl
0.01% Sodium Azide
Immunogen affinity purified


  • Western Blot 1:10000
  • Flow Cytometry 1:10-1:1000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:500-1:2500
  • Immunohistochemistry-Frozen
  • Proximity Ligation Assay
Application Notes
Use in Flow Cytometry reported in scientific literature (PMID: 23682015). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24056230) Use in Proximity Ligation Assay reported in scientific literature (PMID 24470503)
Read Publications using
NB100-1771 in the following applications:

Reactivity Notes

No reaction was observed against Human, or Rat serum proteins. Known Cross Reactivity: rGFP. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants. Reactivity in transgenic mice with GFP. Reactivity in human cell lines transfected will a GFP construct. Mouse reactivity reported in multiple pieces of scientific literature.

Alternate Names for GFP Antibody [FITC]

  • eGFP
  • Enhanced Green Fluorescent Protein
  • GFPuv
  • green fluorescent protein (gfp)
  • Green Fluorescent Protein


Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation. Fluorescein conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NB100-1771)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: FLOW, ICC/IF, IHC-Fr, PLA.

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Showing Publications 1 - 8 of 8.
Publications using NB100-1771 Applications Species
Doerner JF, Delling M, Clapham DE. Ion channels and calcium signaling in motile cilia. Elife. 2015 Dec 09 [PMID:26650848] (IHC-Fr, Mouse) IHC-Fr Mouse
Ntokou A, Klein F, Dontireddy D et al. Characterization of the platelet-derived growth factor receptor a-positive cell lineage during murine late lung development. Am. J. Physiol. Lung Cell Mol. Physiol. 2015 Aug 28 [PMID:26320158] (IHC-Fr) IHC-Fr
Hauser J, Grundstrom C, Grundstrom T. Allelic Exclusion of IgH through Inhibition of E2A in a VDJ Recombination Complex. J. Immunol. 2014 Feb 24 [PMID:24470503] (PLA) PLA
Freeman K, Tao W, Sun H et al. In situ three-dimensional reconstruction of mouse heart sympathetic innervation by two-photon excitation fluorescence imaging. J Neurosci Methods. 2013 Sep 19 [PMID:24056230] (IHC-Fr, ICC/IF) IHC-Fr, ICC/IF
Lampron A, Pimentel-Coelho PM, Rivest S. Migration of bone marrow-derived cells into the CNS in models of neurodegeneration. J Comp Neurol 2013 May 16 [PMID:23682015] (FLOW) FLOW
Hauser J, Sveshnikova N, Wallenius A, Baradaran S, Saarikettu J, Grundstrom T. B-cell receptor activation inhibits AID expression through calmodulin inhibition of E-proteins. PNAS, 2008;105(4):1267-72. 29-Jan. [PMID:18203819]
Yee, Min et al. Type II epithelial cells are critical target for hyperoxia-mediated impairment of postnatal lung development. Am J Physiol Lung Cell Mol Physiol 291: L1101-L1111. 2006 [PMID:16861382]
Dontireddy DA. Identification of pulmonary PDGFRalpha-positive fibroblast specific miRNA and mRNA expression profiles during postnatal lung development Thesis 2015 (ICC/IF, Mouse) ICC/IF Mouse

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFP Antibody (NB100-1771). (Showing 1 - of FAQs).

Secondary Antibodies


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Bioinformatics Tool for GFP Antibody (NB100-1771)

Discover related pathways, diseases and genes to GFP Antibody (NB100-1771). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on GFP.

GFP - Be Green!
Green fluorescence protein (GFP) is a 27KD protein derived from the jellyfish Aquorea victoria that emits a green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP is a highly v...  Read full blog post.

GFP (Green Fluorescent Protein) Bringing Light to Life
GFP (green florescent protein), originally discovered in the jellyfish Aequorea victoria, (1) is one of the most extensively investigated and exploited proteins in the area of life sciences. GFP is well known for its proficiency emit fluorescence and...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

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Gene Symbol GFP

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