GFP Antibody (859) - Azide and BSA Free

Western Blot: GFP Antibody (859) [NBP2-43575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (5 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with GFP antibody (859) diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: GFP Antibody (859) [NBP2-43575] - GFP-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: GFP stained by GFP antibody [859] diluted at 1:2000.Blue: Hoechst 33342 staining.

Western Blot: GFP Antibody (859) [NBP2-43575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GFP antibody [859] diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: GFP Antibody (859) [NBP2-43575] - Analysis of GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFP is expressed in the tranfected cell Red: .GFP-recombinant protein stained by GFP-tag antibody diluted at 1:2000. Blue: Hoechst 33342 staining.

ELISA: GFP Antibody (859) [NBP2-43575] - Coating Ab: mouse anti-GFP (2ug/well) Sample: GFP protein Detected Ab: rabbit anti-GFP Conjugated Ab: goat anti-rabbit IgG-HRP (1:20000)

Immunoprecipitation: GFP Antibody (859) [NBP2-43575] - Analysis of 400 ug cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 ug of GFP tag antibody 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.

• Reactivity: All-NA
• Applications: WB, ELISA, Func, ICC/IF, IHC, IP, IHC
• Clone: 859
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GFP Antibody (859) - Azide and BSA Free Summary

• Immunogen: Full length GFP recombinant protein
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:10000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:2000
  • Immunohistochemistry Whole-Mount 1:10 - 1:500
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100-1:500
  • In vivo assay
  • Sandwich ELISA
  • Western Blot 1:1000-1:10000
• Application Notes: Use in In vivo reported in scientific literature (PMID:34246771).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for GFP Antibody (859) - Azide and BSA Free

• eGFP
• Enhanced Green Fluorescent Protein
• GFP
• GFPuv
• green fluorescent protein (gfp)
• Green Fluorescent Protein

Background

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T., . . . Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S., . . . Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFP Antibody (NBP2-43575)(29)

Publications using NBP2-43575

• Obara K, Reynoso J, Hamada Y et al. Hair follicle associated pluripotent (HAP) stem cells jump from transplanted whiskers to pelage follicles and stimulate hair growth Scientific reports 2022-12-07 [PMID: 36476963] (IHC-P)
• Popescu MA, Patriche D, Dobrica MO et al. Sac1 phosphatidylinositol 4-phosphate phosphatase is a novel host cell factor regulating hepatitis B virus particles assembly and release The FEBS journal 2022-07-11 [PMID: 35816160]
• Tschumi CW, Sharma R, Lynch WB, Beckstead MJ Retrograde neurotensin release from dopamine neurons drives long-term depression of substantia nigra dopamine signaling J Neurosci 2022-07-06 [PMID: 35794014]
• Tu X, Li X, Zhu H et al. Unilateral cerebral ischemia induces morphological changes in the layer V projection neurons of the contralateral hemisphere Neuroscience research 2022-06-28 [PMID: 35777459]
• Chinn HK, Gardell JL, Matsumoto LR et al. Hypoxia-inducible lentiviral gene expression in engineered human macrophages Journal for immunotherapy of cancer 2022-06-01 [PMID: 35728871]
• Tanaka T, Moriya K, Tsunenaga M et al. Visualized procollagen I alpha 1 demonstrates the intracellular processing of propeptides Life science alliance 2022-05-01 [PMID: 35181633]
• Longo LVG, Goodyear EG, Zhang S et al. Involvement of Smi1 in cell wall integrity and glucan synthase Bgs4 localization during fission yeast cytokinesis Molecular biology of the cell 2021-12-15 [PMID: 34910579]
• Dan J, Izumi M, Habuchi H et al. A novel mice model of acute flares in osteoarthritis elicited by intra-articular injection of cultured mast cells Journal of experimental orthopaedics 2021-09-08 [PMID: 34495429] (ICC/IF)
• van der Veldt S Encoding of space and self-motion by GABAergic cells in the lateral septum Thesis 2021-01-01 (IF/IHC)
• Choi SH, Cho K, Kim ES, Yoo HY Proline-serine-threonine-repeat region of MDC1 mediates Chk1 phosphorylation and the DNA double-strand break repair The international journal of biochemistry & cell biology 2021-12-31 [PMID: 34974185] (WB)
• Miihkinen M NOVEL INSIGHTS INTO FILOPODIA FUNCTION Thesis 2021-01-01
• Carlson J, Price L, Cook I, Deng H Drosophila Keap1 xenobiotic response factor regulates developmental transcription through binding to chromatin Developmental biology 2021-10-15 [PMID: 34662537]
• Pisano TJ, Dhanerawala ZM, Kislin M et al. Homologous organization of cerebellar pathways to sensory, motor, and associative forebrain Cell reports 2021-09-21 [PMID: 34551311] (IF/IHC)
• Wu QW, Kapfhammer JP The Bacterial Enzyme RfxCas13d Is Less Neurotoxic Than PspCas13b and Could Be a Promising RNA Editing and Interference Tool in the Nervous System Brain sciences 2021-08-09 [PMID: 34439673] (ICC/IF)
• Moon H, Sung H, Park J, Kwon H Construction of an avian hepatitis E virus replicon expressing heterologous genes and evaluation of its potential as an RNA vaccine platform Korean Journal of Veterinary Research 2021-06-30
• Märkl F, Benmebarek MR, Keyl J et al. Bispecific antibodies redirect synthetic agonistic receptor modified T cells against melanoma Journal for immunotherapy of cancer 2023-05-01 [PMID: 37208128] (IHC-P)
• Rementer, Cameron, C Engineered Myeloid Precursors Differentiate into Osteoclasts and Resorb Heterotopic Ossification in Mice Research Square 2022-11-07 (Immunocytochemistry/ Immunofluorescence)
• Corzo-López A, Leyva-Leyva M, Castillo-Viveros V et al. Molecular Mechanisms of Nuclear Transport of the Neuronal Voltage-gated Ca2+ Channel ?3 Auxiliary Subunit Neuroscience 2023-05-09 [PMID: 37169165]
• Steiert B, Icardi CM, Faris R et al. The Chlamydia trachomatis type III-secreted effector protein CteG induces centrosome amplification through interactions with centrin-2 Proceedings of the National Academy of Sciences of the United States of America 2023-05-16 [PMID: 37155906]
• Lee HJ, Park JH, Trotter JH et al. Reelin and APP Cooperatively Modulate Dendritic Spine Formation In Vitro and In Vivo Experimental neurobiology 2023-02-28 [PMID: 36919335] (Immunocytochemistry/ Immunofluorescence)
• Loiben AM, Chien WM, Friedman CE et al. Cardiomyocyte Apoptosis Is Associated with Contractile Dysfunction in Stem Cell Model of MYH7 E848G Hypertrophic Cardiomyopathy International journal of molecular sciences 2023-03-03 [PMID: 36902340] (WB, Human)
• Seneviratne AMPB, Lidagoster S, Valbuena-Castor S et al. Kinesins Modify ERR1-Dependent Transcription Using a Conserved Nuclear Receptor Box Motif International journal of molecular sciences 2023-02-14 [PMID: 36835206] (WB)
• Kohyama K, Nishida H, Kaneko K et al. Complement-dependent cytotoxicity of human autoantibodies against myelin oligodendrocyte glycoprotein Frontiers in neuroscience 2023-02-01 [PMID: 36816137] (ICC/IF)
• Min H, Youn E, Shim Y Long-Term Caffeine Intake Exerts Protective Effects on Intestinal Aging by Regulating Vitellogenesis and Mitochondrial Function in an Aged Caenorhabditis Elegans Model Nutrients 2021-07-23 [PMID: 34444677]
• Peterson Ar, Garcia Ta, Cullion K Et Al. Targeted overexpression of glutamate transporter-1 reduces seizures and attenuates pathological changes in a Mouse model of epilepsy Neurobiology of disease 2021-07-09 [PMID: 34246771] (WB, In Vivo)
• Pocognoni CA, Viktorova EG, Wright J, Meissner JM. Highly conserved motifs within the large Sec7 ARF guanine nucleotide exchange factor GBF1 target it to the Golgi and are critical for GBF1 activity. Am. J. Physiol., Cell Physiol. 2018-02-14 [PMID: 29443553] (Jellyfish)
• Antoniou A, Khudayberdiev S, Idziak A et al. The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis during development EMBO Rep. [PMID: 29263199] (IP, Jellyfish)
• Loiben A, Chien W, Friedman C et al. Cardiomyocyte apoptosis contributes to contractile dysfunction in stem cell model ofMYH7E848G hypertrophic cardiomyopathy bioRxiv 2023-01-25
• Mar J, Makhijani K, Flaherty D, Bhat KM Nuclear Prospero allows one-division potential to neural precursors and post-mitotic status to neurons via opposite regulation of Cyclin E PLoS genetics 2022-08-01 [PMID: 35939521] (IHC-P, Drosophila)

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