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antibody news

Best genes to knockout to control autophagic flux

Autophagy interactive signaling pathway, Novus Biologicals, macroautophagy induction, autophagosome maturation, microautophagy, autolysosome, elongation

By Christina Towers, PhD

Tags: 
Autophagy
Autophagic flux
LC3
LC3B
LC3-II
Chloroquine
Bafilomycin
Autophagy Inhibitors
ATGs
ATG5
ATG7
Atg3
ATG12
stx17
p62-sqstm1
ATG13
Apoptosis
Christina Towers
autophagosome
Autophagy flux

Make each cell count: How to assess autophagy using flow cytometry

LC3B expression in HeLa cells treated with chloroquine overnight, ICC. Novus Biologicals

Kristy R. Howell, PhD

Tags: 
Autophagy
autophagosome
LC3
LC3B
alpha Tubulin
GFP
mCherry
Autophagy flux
Chloroquine
rapamycin
Flow cytometry
Kristy Howell
Jurkat cell
MIFC
puncta
fluorescence
Immunocytochemistry
Immunofluorescence

How to visualize autophagy by microscopy

LC3B and alpha tubulin expression in HeLa cells treated with Chloroquine, ICC, immunocytochemistry, immunofluorescence

By Christina Towers, PhD

Tags: 
Autophagy
autophagosome
LC3
LC3B
p62/SQSTM1
alpha Tubulin
GFP
Mitophagy
mCherry
Autophagic flux
Chloroquine
Christina Towers
Autophagy flux
microscopy

Best way to quantitatively measure Autophagic Flux

LC3B expression in HeLa cells, ICC, immunocytochemistry, immunofluorescence

By Christina Towers, PhD

Tags: 
Autophagy flux
LC3
LC3B
LC3-II
Chloroquine
Bafilomycin
Autophagy
Autophagy Inhibitors
Christina Towers
autophagosome
GFP
Flow cytometry
ratiometric
RFP
mCherry

Animal Models to Study Autophagy

Autophagy Signaling Pathway, Novus Biologicals, macroautophagy induction, elongation, microautophagy, autophagosome maturation, autolysosome

By Christina Towers, PhD

Tags: 
ULK1
ATG13
rb1cc11
beclin-1
AMBRA1
ATG14
vps14/pik3r4
VPS34
ATG9A
ATG7
Atg10
ATG12
ATG5
ATG4B
Atg3
LC3B
p62-sqstm1
Autophagy
autophagosome
Christina Towers
mCherry
GFP
Zebrafish
Animal models

Application Focus: I see an increase in LC3, now what?

LC3A expression in HeLa cells, chloroquine treated, ICC

 

By Christina Towers, PhD.

 

Tags: 
Autophagy
autophagosome
Transcription Regulation
LC3
LC3B
Bafilomycin
Chloroquine
beta-Actin
Lysosome
Western blot
mCherry
Autophagy flux
Flow cytometry
LC3-II
Christina Towers

Epigenetic Control of Autophagy

G9a expression in human MCF7 cell line ICC, immunocytochemistry, immunofluorescence, IF

By Christina Towers, PhD.

Tags: 
Autophagy
Epigenetics
autophagosome
mTOR
EZH2
TSC2
carm1
tfeb
AMPK
skp2
LC3B
WIPI1
tp53imp2
BRD4
transcription factor E3
mitf
g9a-ehmt2
Autophagic flux
Methylation
Acetylation
DNA methyltransferase
mTORC
Christina Towers

Autophagy inhibition in pediatrics: One physician-scientist’s brave decision

Autophagy Pathway, Novus Biologicals, macroautophagy induction, autophagosome maturation, microautophagy, autolysosome, elongation

By Christina Towers, PhD.

Tags: 
Autophagy
Cancer
ATG
Tumor
Immune System
immunity
T-Cells
LC3B
p62/SQSTM1
Lysosome
Neuroscience
Brain
neurodegeneration
Chloroquine
vemurafenib
oncology
Christina Towers

Five Tips to Successful Western Blot of phospho-IRE1 alpha (Ser724)

1. Sample Preparation
Tags: 
pSer724 IRE1 alpha
Total IRE1 alpha
phospho-IRE1 alpha
IRE1 alpha