![Western Blot: HIF-3 alpha Antibody [NB100-2529] - Detection of HIF-3 alpha using NB100-2529. Lane 1: Cos7 (-) control. Lane 2: Cos7 CoCl2 treated (+) control.](http://images.novusbio.com/fullsize/HIF-3-alpha-Antibody-Western-Blot-NB100-2529-img0006.jpg "Western Blot: HIF-3 alpha Antibody [NB100-2529] - Detection of HIF-3 alpha using NB100-2529. Lane 1: Cos7 (-) control. Lane 2: Cos7 CoCl2 treated (+) control.")
| Immunogen | A synthetic peptide made to an internal region of human HIF-3 alpha (within residues 250-350). [Swiss-Prot: Q66K72] |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HIF3A |
| Purity | Ammonium sulfate precipitation |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | In Western Blot analysis, a band is recognized at ~68 kDa representing HIF-3 alpha. Because a band at ~71 kDa is also seen in hypoxic samples, it is important to run a negative control lysate as well (see image). In ICC/IF, cytoplasmic and nuclear staining was observed in HeLa cells, which is consistent with the literature under normoxic conditions for HIF-3 alpha (PMID: 16775626). |
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| Theoretical MW | 68 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Purity | Ammonium sulfate precipitation |
| Images | Ratings | Applications | Species | Date | Details | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
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reviewed by:
Pramod Mallikarjuna |
WB | Human | 08/30/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for HIF-3 alpha Antibody (NB100-2529)Find related products by research area.
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HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt... Read full blog post. |
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HIF-3 alpha: a versatile target with hypoxia dependent and independent functions By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon... Read full blog post. |
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HIF Prolyl Hydroxylase 2: an important Oxygen Sensor Protein Prolyl hydroxylase domain (PHD) proteins, including PHD1, PHD2, and PHD3, mediate oxygen-dependent degradation of hypoxia-inducible factor (HIF) alpha subunits. Suppression of PHD enzymes leads to stabilization of HIFs and offers a potential treatment... Read full blog post. |
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HIF Antibodies: Beyond HIF-1 alpha The hypoxia inducible factors are a family of heterodimeric transcription factors which are activated in response to lowered oxygen levels, or hypoxia. Although it may seem that HIF-1 alpha receives all the attention, other HIF antibodies, such as the... Read full blog post. |
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A Role for HIF-1 alpha Antibody in Renal Research The Hypoxia Inducible Factors (HIFs) are a family of mammalian transcription factors which are expressed in response to low cellular oxygen concentrations (hypoxia). Three human hypoxia inducible factors have been identified, HIF-1, HIF-2 and HIF-3, e... Read full blog post. |
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| Gene Symbol | HIF3A |
![Immunocytochemistry/Immunofluorescence: HIF-3 alpha Antibody [NB100-2529] - HIF3 alpha antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 549 (red).](http://images.novusbio.com/fullsize/HIF-3-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2529-img0004.jpg "Immunocytochemistry/Immunofluorescence: HIF-3 alpha Antibody [NB100-2529] - HIF3 alpha antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 549 (red).")
![Western Blot: HIF-3 alpha Antibody [NB100-2529] - HIF-3a expression in papillary renal cell carcinoma samples. Image from verified customer review.](http://images.novusbio.com/fullsize/HIF-3-alpha-Antibody-Western-Blot-NB100-2529-img0005.jpg "Western Blot: HIF-3 alpha Antibody [NB100-2529] - HIF-3a expression in papillary renal cell carcinoma samples. Image from verified customer review.")
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