Home » 5'-Nucleotidase/CD73 » 5'-Nucleotidase/CD73 Antibodies » 5'-Nucleotidase/CD73 Antibody (AD2) [PE]

5'-Nucleotidase/CD73 Antibody (AD2) [PE]

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Summary
Product Discontinued
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    • Catalog Number
      NBP2-22354
    • Availability
      Product Discontinued

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5'-Nucleotidase/CD73 Antibody (AD2) [PE] Summary

Immunogen
The mouse monoclonal antibody clone AD2 recognizes CD73, a 70 kDa GPI-anchored 5'-nucleotidase.
Specificity
NBP2-22354 recognizes CD73, a 70 kDa GPI-anchored 5'-nucleotidase expressed predominantly on T and B cell subsets, follicular dendritic cells and endothelial cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NT5E
Purity
Size Exclusion Chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
The reagent is designed for Flow Cytometry analysis of human blood cells using 10 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS and 0.2% (w/v) high grade BSA (protease free)
Preservative
0.09% Sodium Azide
Purity
Size Exclusion Chromatography

Alternate Names for 5'-Nucleotidase/CD73 Antibody (AD2) [PE]

  • 5' nucleotidase (CD73)
  • 5'-NT
  • 5-NT
  • 5'-Nucleotidase
  • 5'-nucleotidase, ecto (CD73)
  • CD73 antigen
  • CD73
  • E5NT
  • EC 3.1.3.5
  • Ecto-5'-nucleotidase
  • eN
  • eNT
  • NT
  • NT55'-nucleotidase
  • NT5E
  • NTE
  • Purine 5-Prime-Nucleotidase

Background

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for 5'-Nucleotidase/CD73 Antibody (NBP2-22354). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

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Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target
By Yoskaly Lazo-Fernandez, PhD Role of Adenosine in the Tumor Microenvironment a Target for Cancer TherapyThe tumor microenvironment (TME) tends to be concentrated in the purine nucleoside adenosine, a direct resu...  Read full blog post.
CD73 (Cluster of differentiation 73, ecto-5'-nucleotidase)
CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble...  Read full blog post.

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Bioinformatics

Gene Symbol NT5E