| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33122-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44857 | |||
| Azide and BSA Free | NBP2-34528-1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-353SS | |||
| Azide and BSA Free | NBP2-80606 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-96140SS | |||
| Azide and BSA Free | NBP2-80599 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB100-64979-0.05mg | |||
| Azide Free | NB100-64979SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-108SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-77477SS | |||
| Azide and BSA Free | NBP2-80624 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-18946SS | |||
![Immunohistochemistry-Frozen: CD8 Antibody [NBP2-29475] - Mouse Ccdc88b expression in colon during DSS-induced colitis. Wild type (WT) mice were either not treated (NT) or given 3% DSS for 5 days followed by 3 days of water. Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at day 8, and stained for or Ccdc88b (red) and CD8 (green). Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29030607/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD8-Antibody-Immunohistochemistry-Frozen-NBP2-29475-img0002.jpg "Immunohistochemistry-Frozen: CD8 Antibody [NBP2-29475] - Mouse Ccdc88b expression in colon during DSS-induced colitis. Wild type (WT) mice were either not treated (NT) or given 3% DSS for 5 days followed by 3 days of water. Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at day 8, and stained for or Ccdc88b (red) and CD8 (green). Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29030607/) licensed under a CC-BY license.")
![Immunohistochemistry-Frozen: CD8 Antibody [NBP2-29475] - Mouse Ccdc88b expression in colon during DSS-induced colitis. Wild type (WT) mice were either not treated (NT) or given 3% DSS for 5 days followed by 3 days of water. aCcdc88b mRNA expression in distal colons of NT (n = 3) or DSS-treated WT mice (n = 3 for each time point) at the indicated days. Data represent expression relative to hprt±SEM (n = 3). *P < 0.05, **P < 0.01 (two-tailed Student’s t-test) & are representative of one experiment. b Representative immunoblots for Ccdc88b protein detected in extracts from distal colons from NT or DSS-treated WT & Ccdc88Bmut mutant mice at indicated time points, & densitometric quantification of Ccdc88b immunoblot normalized to beta -actin±SEM (n = 3) (representative of one of two independent experiments); **P < 0.01 (two-tailed Student’s t-test). c Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at indicated times, & stained with antibodies against Ccdc88b (red), CD45 (green), & E-Cadherin (purple) & nuclei staining DAPI (blue). Scale bars, 100 μm. d Representative FACS plots & quantification of lamina propria cells stained for CD45 & for Ccdc88b antibodies for NT (n = 3) & DSS-treated WT mice (n = 4 for each time point) at indicated time points, data are representative of one experiment. e Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at day 8, & stained for Ccdc88b (red), CD3 (green) & CD11b (green); nuclei are stained with DAPI (blue). Scale bars, 100 μm. f same as in e for Ccdc88b (red), CD4 (green) & CD8 (green). Scale bars, 100 μm Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-01381-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-29475_rabbit-polyclonal-cd8-antibody-imgenex-abx-160a-20ug-310202416113534.jpg)
![Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Western-Blot-NB100-353-img0006.jpg "Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.")
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Immunocytochemistry-Immunofluorescence-NB100-353-img0005.jpg)
![Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - An intracellular stain was performed on HeLa cells with BubR1 Antibody (8G1)NB100-353C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Flow-Cytometry-NB100-353-img0008.jpg)
![Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using BubR1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Flow-Cytometry-NB100-353-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0008.jpg "Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0009.jpg)
![Western Blot: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - Western blot analysis of Bmi1 expression in 1) U2OS and 2) K562 whole cell lysates using NBP1-96140.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Western-Blot-NBP1-96140-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - Bmi1 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0003.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on NIH3T3 cells with BMI-1 Antibody (LLBmi1-1) NBP1-96140 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0012.jpg)
![Western Blot: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - BMI-1 expression in human cell lines (MCF-7, K562 and A459). Image from verified customer review.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Western-Blot-NBP1-96140-img0004.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U2OS cells with BMI-1 Antibody [LLBmi1-1] NBP1-96140AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0005.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U2OS cells with BMI-1 [LLBmi1-1] Antibody NBP1-96140 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0007.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U937 cells with BMI-1 Antibody (LLBmi1-1) NBP1-96140 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979] - Analysis of TfR (Transferrin R) Antibody on mouse brain endothelial cells. Mouse brain endothelial (bEnd.3) cells expressing Transferrin (FICT, green) and nuclei (DAPI, blue). Experimental details: 0.1 % triton, BSA 30 min, Primary Ab: 1:50, 1h, RT, Secondary Ab: AlexaFluor 488 chicken anti-rat IgG (H+L), 1:800, 30 min. Image from verified customer review.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-8D3-Immunocytochemistry-Immunofluorescence-NB100-64979-img0002.jpg "Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979] - Analysis of TfR (Transferrin R) Antibody on mouse brain endothelial cells. Mouse brain endothelial (bEnd.3) cells expressing Transferrin (FICT, green) and nuclei (DAPI, blue). Experimental details: 0.1 % triton, BSA 30 min, Primary Ab: 1:50, 1h, RT, Secondary Ab: AlexaFluor 488 chicken anti-rat IgG (H+L), 1:800, 30 min. Image from verified customer review.")
![Flow Cytometry: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979] - RPE conjugated rat anti mouse Ter-119 and FITC conjugated Rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse Ter-119 and FITC conjugated rat anti mouse CD71. All experiments performed on mouse bone marrow. Data acquired on the ZE5Cell Analyzer](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-8D3-Flow-Cytometry-NB100-64979-img0001.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) CCNA2 is commonly downregulated. The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: * p Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody (NBP1-31330) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-31330_rabbit-polyclonal-cyclin-a2-antibody-immunocytochemistry-immunofluorescence-141120251833319.jpg "Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) CCNA2 is commonly downregulated. The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: * p Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody (NBP1-31330) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.")
![Western Blot: NTH1 Antibody [NB100-108] - Detection of NTH1 (37 kDa) from Hela & K562 cell extracts using NB100-108 (1:500). WB data courtesy of Mark Kelly, Indiana Univ.](http://images.novusbio.com/fullsize/NTH1-Antibody-Western-Blot-NB100-108-img0005.jpg "Western Blot: NTH1 Antibody [NB100-108] - Detection of NTH1 (37 kDa) from Hela & K562 cell extracts using NB100-108 (1:500). WB data courtesy of Mark Kelly, Indiana Univ.")
![Immunocytochemistry/Immunofluorescence: NTH1 Antibody [NB100-108] - NTH1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-108-img0006.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human colon adenocarcinoma using NTH1 antibody at 5ug/ml concentration (1:200 dilution). The primary antibody binding to NTH1/ NTHL1 antigen was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. The NTH1 antibody generated an expected nuclear cytoplasmic staining of NTH1 protein in colon cancer cells as well as the cells of tumor stroma including cancer associated fibroblasts. The nuclear staining of NTH1 was very strong in a sub-set of colon cancer cells.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0014.jpg)
![Western Blot: NTH1 Antibody [NB100-108] - Analysis of NTH1 expression in 1) HeLa, 2) A-431, 3) MCF7 whole cell lysates.](http://images.novusbio.com/fullsize/NTH1-Antibody-Western-Blot-NB100-108-img0002.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human lymph node cancer using 5 ug/ml concentration of NTH1 antibody. The representative image shows strong nuclear and cytoplasmic positivity of NTH1 protein in the lymph node cancer cells.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0013.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human kidney cancer using 5 ug/ml concentration of NTH1 antibody. The renal cancer cells showed nuclear and cytoplasmic positivity for NTH1 protein, whereas, the tumor stroma was largely negative for immunostaining.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0012.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human rectal cancer using 5 ug/ml concentration of NTH1 antibody. The rectal cancer cells as well as the goblet cells in glandular areas showed nuclear-cytoplasmic positivity for NTH1 protein. The cells of tumor stroma did not develop immunostaining for this protein.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0011.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human rectal cancer using 5 ug/ml concentration of NTH1 antibody. This representative image at high resolution depicts nuclear-cytoplasmic positivity for NTH1 protein in rectal cancer cells and the goblet cells. The cells of tumor stroma did not develop any immunostaining for this protein.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0010.jpg)
![Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-NB100-563-img0006.jpg "Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAD2L1 antibody NB100-563 (lot 3) used for IP at 3 ug/mg lysate. MAD2L1 was also immunoprecipitated by a previous lot (lot 2) and by rabbit anti-MAD2L1 antibody NB100-564. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunoprecipitation-NB100-563-img0007.jpg)
![Western Blot: Pax2 Antibody (3C7) [H00005076-M01] - Analysis of PAX2 expression in transfected 293T cell line by PAX2 monoclonal antibody (M01), clone 3C7.Lane 1: PAX2 transfected lysate(47.41 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Pax2-Antibody-3C7-Western-Blot-H00005076-M01-img0006.jpg "Western Blot: Pax2 Antibody (3C7) [H00005076-M01] - Analysis of PAX2 expression in transfected 293T cell line by PAX2 monoclonal antibody (M01), clone 3C7.Lane 1: PAX2 transfected lysate(47.41 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Pax2 Antibody (3C7) [H00005076-M01] - Analysis of monoclonal antibody to PAX2 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 0.5 ug/ml.](http://images.novusbio.com/fullsize/Pax2-Antibody-3C7-Immunohistochemistry-Paraffin-H00005076-M01-img0004.jpg)
![ELISA: Pax2 Antibody (3C7) [H00005076-M01] - Detection limit for recombinant GST tagged PAX2 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Pax2-Antibody-3C7-ELISA-H00005076-M01-img0007.jpg)
![Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse B220/CD45R (Catalog # FAB1217P) and Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # NB100-77477, top panel) or isotype control antibody (Catalog # MAB0061, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0003.jpg "Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse B220/CD45R (Catalog # FAB1217P) and Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # NB100-77477, top panel) or isotype control antibody (Catalog # MAB0061, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).")
![Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Identification of CD117/c-kit expressing bone marrow cells.Double labeling of mouse bone marrow cells with anti-CD45 antibody and rabbit anti-CD117/c-kit antibody 2B8. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0071798) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0004.jpg)
![Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HPi2 Antibody (HIC1-2B4.2B) [NBP1-18946] - Immunofluorescence on frozen section of human pancreas.](http://images.novusbio.com/fullsize/HPi2-Antibody-HIC1-2B4-2B-Immunocytochemistry-Immunofluorescence-NBP1-18946-img0009.jpg "Immunocytochemistry/Immunofluorescence: HPi2 Antibody (HIC1-2B4.2B) [NBP1-18946] - Immunofluorescence on frozen section of human pancreas.")
![Flow Cytometry: HPi2 Antibody (HIC1-2B4.2B) [NBP1-18946] - Analysis of enzyme dispersed human pancreas cells.](http://images.novusbio.com/fullsize/HPi2-Antibody-HIC1-2B4-2B-Flow-Cytometry-NBP1-18946-img0008.jpg)
![Western Blot: DOT1L Antibody [NB100-40845] - DOT1L is involved in UV-survival in mammalian cells. Fifty ug of whole cell extracts from MEFWT or MEFDOT1L cells were resolved by SDS-PAGE and Western-blotted for DOT1L. Asterisk indicates an unspecific cross-reacting band. Ten ug of fractions from histone acid-extraction performed on MEFWT or MEFDOT1L cells were resolved by SDS-PAGE and Western-blotted for H3, H3K79me1 or H3K79me3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1003611), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DOT1L-Antibody-Western-Blot-NB100-40845-img0014.jpg "Western Blot: DOT1L Antibody [NB100-40845] - DOT1L is involved in UV-survival in mammalian cells. Fifty ug of whole cell extracts from MEFWT or MEFDOT1L cells were resolved by SDS-PAGE and Western-blotted for DOT1L. Asterisk indicates an unspecific cross-reacting band. Ten ug of fractions from histone acid-extraction performed on MEFWT or MEFDOT1L cells were resolved by SDS-PAGE and Western-blotted for H3, H3K79me1 or H3K79me3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1003611), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: DOT1L Antibody [NB100-40845] - Human lung carcinoma. Antibody: Affinity purified rabbit anti-DOT1L used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/DOT1L-Antibody-Immunohistochemistry-Paraffin-NB100-40845-img0009.jpg)
![Chromatin Immunoprecipitation: DOT1L Antibody [NB100-40845] - Chromatin from KOPN-8 cell line was immunoprecipitated with anti-DOT1L antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of DOT1L binding within a 250 Kb region of chromosome 10 as detected using anti-DOT1L antibody.](http://images.novusbio.com/fullsize/DOT1L-Antibody-Chromatin-Immunoprecipitation-NB100-40845-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: DOT1L Antibody [NB100-40845] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-DOT1L antibody NB100-40845 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B).](http://images.novusbio.com/fullsize/DOT1L-Antibody-Western-Blot-NB100-40845-img0007.jpg)
![Western Blot: DOT1L Antibody [NB100-40845] - siRNA mediated gene silencing of DOT1L validated by Q-PCR and western blot. The results show a down regulation of DOT1L mRNA expression. Empty bars are untreated cells and solid bars are transfected cells. Results from HSkMSC cultures derived from three different experiments on cells at passage three, Graph is presenting means +/- SEM. *, p-value < 0.05 siRNA treated cells compared to controls Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/s12931-017-0525-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DOT1L-Antibody-Western-Blot-NB100-40845-img0015.jpg)
