TfR (Transferrin R) Antibody (8D3) - Azide and BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (8D3) [NB100-64979] - Analysis of TfR (Transferrin R) Antibody on mouse brain endothelial cells. Mouse brain endothelial (bEnd.3) cells expressing ...read more
Flow Cytometry: TfR (Transferrin R) Antibody (8D3) [NB100-64979] - RPE conjugated rat anti mouse Ter-119 and FITC conjugated Rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse Ter-119 and FITC ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, Func, ICC/IF, IHC, IHC-Fr, In vivo, CyTOF-ready
Clone
8D3
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

TfR (Transferrin R) Antibody (8D3) - Azide and BSA Free Summary

Immunogen
Mouse transformed endothelioma cell line t.end1.
Marker
Recycling Endosome Marker
Specificity
NB100-64979 recognizes native, soluble and denatured forms of murine CD71. The antibody has been used as a BBB transporter vector in mice and is suitable for studying CD71 expression, and iron uptake into different tissues, in the mouse.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
TFRC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100-1:200
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • In vivo assay
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Immunocytochemistry/Immunofluorescence and In vivo assay were reported in scientific literature.
Reviewed Applications
Read 1 Review rated 2
using
NB100-64979 in the following applications:

Publications
Read Publications using
NB100-64979 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Notes

Endotoxin Level <0.01 Eu/ug

Alternate Names for TfR (Transferrin R) Antibody (8D3) - Azide and BSA Free

  • CD71 antigen
  • CD71
  • CD71TRFR
  • p90
  • T9
  • TfR (Transferrin R)
  • TfR (TransferrinR)
  • TfR
  • TFR1
  • TFRC
  • TR
  • transferrin receptor (p90, CD71)
  • transferrin receptor protein 1
  • TRFR

Background

Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumor cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro. The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anemia and the anemia of chronic disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TfR (Transferrin R) Antibody (NB100-64979)(11)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Flow, ICC/IF, IHC, IHC-Fr, In vivo.


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Flow
(1)
ICC/IF
(1)
IHC
(3)
IHC-Fr
(1)
In vivo
(2)
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Mouse
(6)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-64979 Applications Species
Masuda T, Amann L, Monaco G et al. Specification of CNS macrophage subsets occurs postnatally in defined niches Nature Apr 1 2022 [PMID: 35444273] (IHC-Fr, Mouse) IHC-Fr Mouse
Li D, Peng J, Li T et al. Itgb3-integrin-deficient mice may not be a sufficient model for patients with Glanzmann thrombasthenia Molecular medicine reports Jun 1 2021 [PMID: 33880575]
Yang AC, Stevens MY, Chen MB et al. Physiological blood-brain transport is impaired with age by a shift in transcytosis Nature Jul 1 2020 [PMID: 32612231] (IHC, Mouse) IHC Mouse
Meister SW, Hjelm LC, Dannemeyer M et al. An Affibody Molecule Is Actively Transported into the Cerebrospinal Fluid via Binding to the Transferrin Receptor Int J Mol Sci Apr 23 2020 [PMID: 32340383] (Flow) Flow
Yanagida K, Engelbrecht E, Niaudet C et al. Sphingosine 1-Phosphate Receptor Signaling Establishes AP-1 Gradients to Allow for Retinal Endothelial Cell Specialization Dev. Cell Mar 23 2020 [PMID: 32059774]
DarYadel A, Bettoni C, Haider T et al. Erythropoietin stimulates fibroblast growth factor 23 (FGF23) in mice and men. Pflugers Arch. Jul 2 2018 [PMID: 29961920] (IHC, Mouse) IHC Mouse
Papademetriou IT, Garnacho C, Schuchman EH, Muro S. In vivo performance of polymer nanocarriers dually-targeted to epitopes of the same or different receptors. Biomaterials 2013 Feb 8 [PMID: 23398883] (In vivo, Mouse) In vivo Mouse
Papademetriou J, Garnacho C, Serrano D et al. Comparative binding, endocytosis, and biodistribution of antibodies and antibody-coated carriers for targeted delivery of lysosomal enzymes to ICAM-1 versus transferrin receptor J Inherit Metab Dis 2012 Sep 12 [PMID: 22968581] (In vivo, Mouse) In vivo Mouse
Ruderisch N, Virgintino D, Makrides V, Verrey F. Differential axial localization along the mouse brain vascular tree of luminal sodium-dependent glutamine transporters Snat1 and Snat3 J Cereb Blood Flow Metab 2011 Jul [PMID: 21364602] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Lee, HJ et al. Targeting rat anti-mouse transferin receptor monoclonal antibodies through blood-brain barrier in mouse. J Pharmacol Exp Ther 292: 1048-52. 2000 [PMID: 10688622]
Show All 11 Publications.

Review for TfR (Transferrin R) Antibody (NB100-64979) (1) 21

Average Rating: 2
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-64979:
Filter by Applications
ICC
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry TfR (Transferrin R) NB100-64979
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2
reviewed by:
Verified Customer
ICC Mouse 01/14/2022
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Summary

ApplicationImmunocytochemistry
Sample TestedbEnd.3 mouse endothelioma cell line
SpeciesMouse
Lot150030

Comments

Comments***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
0.1 % triton
BSA 30 min
Primary Ab: 1:50, 1h, RT
Secondary Ab: AlexaFluor 488 chicken anti-rat IgG (H+L), 1:800, 30 min

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Blogs on TfR (Transferrin R).

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

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Verified Customer
01/14/2022
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol TFRC