STRO-1 Antibody (STRO-1)

Images

 
Immunocytochemistry/ Immunofluorescence: STRO-1 Antibody (STRO-1) [NBP1-48356] - MG-63 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells ...read more
Flow Cytometry: STRO-1 Antibody (STRO-1) [NBP1-48356] - STRO-1 antibody was tested at 1:400 in MG-63 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).
Immunocytochemistry/ Immunofluorescence: STRO-1 Antibody (STRO-1) [NBP1-48356] - Antibody was tested at 1:50 in MG-63 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF
Clone
STRO-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

STRO-1 Antibody (STRO-1) Summary

Immunogen
Human CD34+ bone marrow cells.
Marker
Stromal Cell Surface Marker
Isotype
IgM Lambda
Clonality
Monoclonal
Host
Mouse
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:400
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Application Notes
This STRO-1 Antibody is useful for Immunocytochemistry/Immunofluorescence and Flow Cytometry.
Publications
Read Publications using
NBP1-48356 in the following applications:

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgM purified

Alternate Names for STRO-1 Antibody (STRO-1)

  • STRO1
  • STRO-1

Background

As a part of experimental investigations focused on identifying reliable markers for mesenchymal stromal/stem cells (MSC), STRO-1 antibody was generated by using one of the several hybridomas that were generated by immunizing mice intrasplenically with human CD34+ bone marrow cells and despite lack of much details on exact identity of its cognate antigen, STRO-1 antibody has been heavily relied upon for the recognition/isolation of various types of MSCs for over past two decades. After initial screening against T-/B-cell lines, hybridomas followed further selection for reactivity against subpopulations of CD34-expressing cells as well as for the identification of STRO-1 clone, and additional tests identified STRO-1 antibody as of the IgM isotype which reacts with stromal cells in the adherent layer of long-term bone marrow cultures. STRO-1 has been considered the best-known MSC marker and its antibody has been employed primarily for FLOW application as well as for staining of putative MSCs. However, with the use of IHC and Western blot applications, recent studies have identified STRO-1 as a 75kd endothelial antigen in the vasculature of liver, lung and kidney tissues wherein it co-localized with endothelial marker vWF and also detected it in certain cancer cell lines namely PC3, DU145, MCF7, and K562.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STRO-1 Antibody (NBP1-48356)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: FLOW, ICC/IF.


Filter By Application
FLOW
(3)
ICC/IF
(5)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-48356 Applications Species
Chen YY, He ST, Yan FH et al. Dental pulp stem cells express tendon markers under mechanical loading and are a potential cell source for tissue engineering of tendon-like tissue. Int J Oral Sci. Nov 4 2016 [PMID: 27811845] (Human) Human
Homayounfar N, Verma P, Nosrat A et al. Isolation, Characterization, and Differentiation of Dental Pulp Stem Cells in Ferrets. J Endod. 2016 Jan 12 [PMID: 26794343] (ICC/IF, Human) ICC/IF Human
Li Y, Yan M, Wang Z et al. 17beta-estradiol promotes the odonto/osteogenic differentiation of stem cells from apical papilla via mitogen-activated protein kinase pathway. Stem Cell Res Ther. 2014 Nov 17 [PMID: 25403930] (ICC/IF, Human) ICC/IF Human
Li J, Yan M, Wang Z et al. Effects of Canonical NF- kappaB Signaling Pathway on the Proliferation and Odonto/Osteogenic Differentiation of Human Stem Cells from Apical Papilla. Biomed Res Int. 2014 May 27 [PMID: 24864235] (ICC/IF, FLOW, Human) ICC/IF, FLOW Human
Wang Y, Zheng Y, Wang Z et al. 10-7 m 17 beta-oestradiol enhances odonto/osteogenic potency of Human dental pulp stem cells by activation of the NF-kB pathway. Cell Prolif. 2013 Oct 24 [PMID: 24152244] (ICC/IF, Human) ICC/IF Human
Simmons PJ, Torok-Storb B. Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1. Blood;78(1):55-62. 1991 Jul 1. [PMID: 2070060] (ICC/IF, FLOW, Human) ICC/IF, FLOW Human
Simmons PJ, Torok-Storb B. CD34 expression by stromal precursors in normal human adult bone marrow. Blood;78(11):2848-53. 1991 Dec 1. [PMID: 1720038] (FLOW, Human) FLOW Human

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Product General Protocols

View specific protocols for STRO-1 Antibody (NBP1-48356): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-48356AF405
Alexa Fluor 488 Labeled NBP1-48356AF488
Alexa Fluor 647 Labeled NBP1-48356AF647
Alexa Fluor 700 Labeled NBP1-48356AF700
Allophycocyanin Labeled NBP1-48356APC
Biotin Labeled NBP1-48356B
DyLight 350 Labeled NBP1-48356UV
DyLight 405 Labeled NBP1-48356V
DyLight 488 Labeled NBP1-48356G
DyLight 550 Labeled NBP1-48356R
DyLight 650 Labeled NBP1-48356C
DyLight 680 Labeled NBP1-48356FR
DyLight 755 Labeled NBP1-48356IR
FITC Labeled NBP1-48356F
PE Labeled NBP1-48356PE
PerCP Labeled NBP1-48356PCP

Additional STRO-1 Products

STRO-1 NBP1-48356

Research Areas for STRO-1 Antibody (NBP1-48356)

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