STRO-1 Antibody (STRO-1) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: STRO-1 Antibody (STRO-1) - Azide and BSA Free [NBP2-80966] - MG-63 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-STRO-1 (STRO-1) at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.

Flow Cytometry: STRO-1 Antibody (STRO-1) - Azide and BSA Free [NBP2-80966] - STRO-1 antibody was tested at 1:400 in MG-63 cells using an Alexa Fluor 488 secondary (green) alongside unstained cells (red). Image from the standard format of this antibody.

Immunocytochemistry: STRO-1 Antibody (STRO-1) - Azide and BSA Free [NBP2-80966] - Characterization of SCAPs: isolated SCAPs were positive for STRO-1 by immunocytochemistry; PBS served as a negative control. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2014/319651/), licensed under a CC-BY license. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: STRO-1 Antibody (STRO-1) - Azide and BSA Free [NBP2-80966] - Antibody was tested at 1:50 in MG-63 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red). Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC, CyTOF-ready
• Clone: STRO-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

STRO-1 Antibody (STRO-1) - Azide and BSA Free Summary

• Immunogen: Human CD34+ bone marrow cells.
• Isotype: IgM Lambda
• Clonality: Monoclonal
• Host: Mouse
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:400
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
• Application Notes: This STRO-1 Antibody is useful for Immunocytochemistry/Immunofluorescence and Flow Cytometry.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: IgM purified

Alternate Names for STRO-1 Antibody (STRO-1) - Azide and BSA Free

• STRO1
• STRO-1

Background

As a part of experimental investigations focused on identifying reliable markers for mesenchymal stromal/stem cells (MSC), STRO-1 antibody was generated by using one of the several hybridomas that were generated by immunizing mice intrasplenically with human CD34+ bone marrow cells and despite lack of much details on exact identity of its cognate antigen, STRO-1 antibody has been heavily relied upon for the recognition/isolation of various types of MSCs for over past two decades. After initial screening against T-/B-cell lines, hybridomas followed further selection for reactivity against subpopulations of CD34-expressing cells as well as for the identification of STRO-1 clone, and additional tests identified STRO-1 antibody as of the IgM isotype which reacts with stromal cells in the adherent layer of long-term bone marrow cultures. STRO-1 has been considered the best-known MSC marker and its antibody has been employed primarily for FLOW application as well as for staining of putative MSCs. However, with the use of IHC and Western blot applications, recent studies have identified STRO-1 as a 75kd endothelial antigen in the vasculature of liver, lung and kidney tissues wherein it co-localized with endothelial marker vWF and also detected it in certain cancer cell lines namely PC3, DU145, MCF7, and K562.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.