| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56724SS | |||
| Azide and BSA Free | NBP2-33247 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2024AF405 | |||
| Alexa Fluor 488 | NB21-2024AF488 | |||
| Alexa Fluor 647 | NB21-2024AF647 | |||
| Alexa Fluor 700 | NB21-2024AF700 | |||
| Biotin | NB21-2024B | |||
| DyLight 405 | NB21-2024V | |||
| DyLight 488 | NB21-2024G | |||
| DyLight 550 | NB21-2024R | |||
| DyLight 650 | NB21-2024C | |||
| FITC | NB21-2024F | |||
| HRP | NB21-2024H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2024SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56603SS | |||
| Azide and BSA Free | NBP2-31099-1.0mg | |||
| Azide and BSA Free | NBP2-31099-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-81946-0.025ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2077AF405 | |||
| Alexa Fluor 488 | NB21-2077AF488 | |||
| Alexa Fluor 647 | NB21-2077AF647 | |||
| Alexa Fluor 700 | NB21-2077AF700 | |||
| Biotin | NB21-2077B | |||
| DyLight 405 | NB21-2077V | |||
| DyLight 488 | NB21-2077G | |||
| DyLight 550 | NB21-2077R | |||
| DyLight 650 | NB21-2077C | |||
| FITC | NB21-2077F | |||
| HRP | NB21-2077H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2077SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62566-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84578-25ul | |||
![Western Blot: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Western-Blot-NB300-619-img0029.jpg "Western Blot: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates.")
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - GR94 in human primary fibroblasts, ER staining. Primary antibody incubation: 1:250 in 10% Serum in PBST (0.1% Triton) O/N 4C. Secondary: Donkey anti-rat 647 1:500 2h RT. ICC/IF image submitted by a verifed customer review.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunocytochemistry-Immunofluorescence-NB300-619-img0032.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse breast tissue.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunohistochemistry-Paraffin-NB300-619-img0028.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Western-Blot-NB300-619-img0030.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) shows staining in C6 Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunocytochemistry-Immunofluorescence-NB300-619-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) shows staining in Hela Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunocytochemistry-Immunofluorescence-NB300-619-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) shows staining in NIH-3T3 Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunocytochemistry-Immunofluorescence-NB300-619-img0019.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse liver tissue.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunohistochemistry-Paraffin-NB300-619-img0021.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse lymph node.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunohistochemistry-Paraffin-NB300-619-img0023.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 (Grp94) in U2OS cells. Cells fixed with 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature and blocked with 2% BSA in PBST (from Product # 37525) for 30 minutes at room temperature. Cells were treated with Peroxidase Suppressor, probed with a Grp94 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBST, and incubated with an HRP-conjugated goat anti-rat IgG (H+L) secondary antibody at a dilution of 1:1000 for 30 minutes at room temperature. Chromogenic detection was performed using Metal Enhanced DAB Substrate Kit.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunohistochemistry-Paraffin-NB300-619-img0026.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Staining on poorly differentiated adenocarcinoma from paraffin-embedded human colon tissue sections.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-9G10-Immunohistochemistry-Paraffin-NB300-619-img0027.jpg)
![Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NB100-56724] - Analysis using the Biotin conjugate of NB100-56724. Detection of Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with this antibody.](http://images.novusbio.com/fullsize/IkB-alpha-p-Ser32-p-Ser36-Antibody-39A1413-Western-Blot-NB100-56724-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NB100-56724] - Analysis using the Biotin conjugate of NB100-56724. Detection of Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with this antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NB100-56724] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal) - a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lane 2) or without 1 nM TNF-a (lane 1). The membrane was blotted with NB100-56724 and immunoreactivity was detected by ECL. The data shows that NB100-56724 detects specifically the phosphorylated form of IkBa.](http://images.novusbio.com/fullsize/IkB-alpha-p-Ser32-p-Ser36-Antibody-39A1413-Western-Blot-NB100-56724-img0001.jpg)
![Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in NIH 3t3 histone prep.](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys5]-Antibody-Western-Blot-NB21-2024-img0010.jpg "Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in NIH 3t3 histone prep.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5] Antibody [NB21-2024] - Histone H4 K5ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys5]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2024-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys5] Antibody [NB21-2024] - 2 ug of NB21-2024 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys5]-Antibody-Chromatin-Immunoprecipitation-NB21-2024-img0008.jpg)
![Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in HeLa histone prep.](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys5]-Antibody-Western-Blot-NB21-2024-img0009.jpg)
![Histone H4 [ac Lys5] Antibody [NB21-2024]](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5]-Antibody-NB21-2024-img0011.jpg)
![Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi](http://images.novusbio.com/fullsize/Androgen-R-NR3C4-p-Ser213-p-Ser210-Antibody-156C135-2-Western-Blot-NB100-56603-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi")
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Affinity Purified TOR/TOR/mTOR [p Ser2448] antibody is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human TOR/mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0010.jpg "Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Affinity Purified TOR/TOR/mTOR [p Ser2448] antibody is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human TOR/mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.")
![Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody [NB600-607] - affinity purified TOR/TOR/mTOR [p Ser2448] antibody was used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney (40X). Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-NB600-607-img0011.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Rapamycin reduces mTOR phosphorylation induced by TII. Whole-cell lysates were prepared from astrocytes activated with TII for 2 h. 10 nM Rapamycin was added at the beginning of the experiment and equal amounts of protein were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR) (upper gel) and consequently for beta-actin (lower gel). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0006.jpg)
![Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Brown arrow= IBa1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and IBA1 positive cells. IHC-P Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-Paraffin-NB600-607-img0005.jpg)
![TOR/mTOR [p Ser2448] Antibody [NB600-607]](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-N-A-NB600-607-img0008.jpg)
![ELISA: TOR/mTOR [p Ser2448] Antibody [NB600-607] - ELISA Results of TOR/TOR/mTOR [p Ser2448] antibody tested against BSA-conjugated non-phospho [purple line] and phospho [blue line] forms of immunizing peptide. Each well was coated in duplicate with either 0.1ug of conjugate. The working dilution is 1:37,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP conjugate stabilizer, Goat Anti-Rabbit HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-ELISA-NB600-607-img0012.jpg)
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Analysis of mTOR phosphorylation during microglial activation(A) Whole-cell lysates were prepared from microglial cells incubated for 2 h as indicated. Equal amounts of proteins were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR), upper gel. The same blots were subsequently probed for beta -actin, lower gel. (B) Quantitation of densitometry where p-mTOR values are reflected relative to those for beta -actin. Data are expressed as means±S.E.M. of n=1 replicate for each group, each assayed in triplicates. Representative of two different experiments. Data were analyzed by one-way ANOVA followed by the Bonferroni's post hoc test. ***P<0.001 versus Control, °P<0.05 versus C-CM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24689533), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-607_rabbit-polyclonal-tor-mtor-p-ser2448-antibody-31020241535647.jpg)
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Analysis of mammalian target of rapamycin (mTOR) phosphorylation during microglial activation. Whole-cell lysates were prepared from microglial cells activated by control-conditioned medium (C-CM) (A) or CM from glioma cells activated with lipopolysaccharide (LPS)/IFN gamma (LI-CM) (B). Equal amounts of proteins were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR), upper gel & were subsequently probed for beta -actin, lower gel. Quantitation of densitometry where p-mTOR values are reflected relative to those for beta -actin. Data are expressed as means ± standard error of the mean of n = 1 replicate for each group, each assayed in triplicate: representative of two different experiments. Data were analyzed by one-way analysis of variance followed by Bonferroni post hoc test. ***P <0.001 versus control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25051975), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-607_rabbit-polyclonal-tor-mtor-p-ser2448-antibody-310202416171492.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-23420241523259.jpg "Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.")
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-23420241523253.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-23420241523256.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-23420241523250.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, NB100-81946 was diluted at 1:200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-2342024155514.jpg)
![Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Western blot analysis of GSK-3 beta [p Tyr279, p Tyr216] expression in mouse testis (A), rat testis (B), rat spleen (C) whole cell lysates.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-234202415232519.jpg)
![Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Analysis of extracts of various cell lines, using NB100-81946](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-103202523472617.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81946(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-113202594524.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Methanol-fixed MEF cells.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-113202515145457.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Staining of Methanol-fixed Hela cells.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-113202515145471.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Methanol-fixed MEF cells](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser536]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82088-img0009.jpg "Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Methanol-fixed MEF cells")
![Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Paraffin-embedded human breast carcinoma tissue.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser536]-Antibody-Immunohistochemistry-Paraffin-NB100-82088-img0007.jpg)
![Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Paraffin-embedded human Lung carcinoma tissue](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser536]-Antibody-Immunohistochemistry-Paraffin-NB100-82088-img0008.jpg)
![Western Blot: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Western Blot analysis of various cells using NB100-82088 diluted at 1:2000](http://images.novusbio.com/fullsize/nb100-82088_rabbit-polyclonal-rela-nfkb-p65-p-ser536-antibody-23420241545352.jpg)
![Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Immunofluorescence staining of methanol-fixed Hela cells using NB100-82088.](http://images.novusbio.com/fullsize/nb100-82088_rabbit-polyclonal-rela-nfkb-p65-p-ser536-antibody-103202523464512.jpg)
![Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Analysis of paraffin-embedded human breast carcinoma tissue using NB100-82088 incubated with blocking peptide (right)](http://images.novusbio.com/fullsize/nb100-82088_rabbit-polyclonal-rela-nfkb-p65-p-ser536-antibody-103202523464555.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - Western Blot using NB21-2077 of porcine whole ovarian homogenate. Image submitted via verified customer review.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys16]-Antibody-Western-Blot-NB21-2077-img0008.jpg "Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - Western Blot using NB21-2077 of porcine whole ovarian homogenate. Image submitted via verified customer review.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys16] Antibody [NB21-2077] - Histone H4 K16ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys16]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2077-img0005.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - WB analysis of H4K16ac in 1) HeLa histone prep lysate and 2) NIH 3T3 histone prep lysate.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys16]-Antibody-Western-Blot-NB21-2077-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - Dot blot analysis of H4K16ac in picomoles of protein.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys16]-Antibody-Dot-Blot-NB21-2077-img0004.jpg)
![Histone H4 [ac Lys16] Antibody [NB21-2077]](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys16]-Antibody-NB21-2077-img0006.jpg)
![Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using NB100-82086](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunohistochemistry-Paraffin-NB100-82086-img0009.jpg "Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using NB100-82086")
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Staining of methanol-fixed Hela cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82086-img0014.jpg)
![Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Human breast carcinoma tissue.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunohistochemistry-Paraffin-NB100-82086-img0010.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Western Blot analysis of various cells using NB100-82086 diluted at 1:1000](http://images.novusbio.com/fullsize/nb100-82086_rabbit-polyclonal-rela-nfkb-p65-p-ser276-antibody-23420241545358.jpg)
![Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Analysis of paraffin- embedded human breast carcinoma tissue using NB100-82086 incubated with blocking peptide (right)](http://images.novusbio.com/fullsize/nb100-82086_rabbit-polyclonal-rela-nfkb-p65-p-ser276-antibody-103202523464543.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with TNF using NB100-82086](http://images.novusbio.com/fullsize/nb100-82086_rabbit-polyclonal-rela-nfkb-p65-p-ser276-antibody-11320251555922.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Lane 1: HEK293. Lane 2: Lentivirus mediated NFkB overexpression. We image submitted by a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-82086_rabbit-polyclonal-rela-nfkb-p65-p-ser276-antibody-western-blot-1132025204015..jpg)
![Western Blot: APP Antibody (6E10) - Chimeric [NBP2-62566] - Anti-Amyloid beta antibody 6E10. Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 (NBP2-62566) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). NBP2-62566 successfully detected aggregate human Amyloid beta in human brain cerebral cortex, frontal cortex and cerebellum lysate samples.](http://images.novusbio.com/fullsize/APP-Antibody-6E10-Chimeric-Western-Blot-NBP2-62566-img0002.jpg "Western Blot: APP Antibody (6E10) - Chimeric [NBP2-62566] - Anti-Amyloid beta antibody 6E10. Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 (NBP2-62566) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). NBP2-62566 successfully detected aggregate human Amyloid beta in human brain cerebral cortex, frontal cortex and cerebellum lysate samples.")
![Immunohistochemistry-Paraffin: APP Antibody (6E10) - Chimeric [NBP2-62566] - Staining of human Alzheimer's disease hippocampus tissue using anti-Amyloid Beta antibody. 6E10 Anti-Amyloid Beta staining of paraffin embedded human hippocampus affected by Alzheimer's disease using the rabbit-chimeric version of 6E10 (NBP2-62566). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62566 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of amyloid plaques in the parahippocampal gyrus may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/APP-Antibody-6E10-Chimeric-Immunohistochemistry-Paraffin-NBP2-62566-img0001.jpg)
![Immunohistochemistry-Frozen: APP Antibody (6E10) - Chimeric [NBP2-62566] - 6E10 staining reveals amyloid plaque in frontal cortex of AD mouse model. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-62566_rabbit-monoclonal-app-antibody-6e10-chimeric-immunohistochemistry-frozen-284202314243..jpg)
![Immunohistochemistry-Frozen: APP Antibody (6E10) - Chimeric [NBP2-62566] - Plaque staining in AD Mouse model (Hippocampus) - without formic acid pretreatment. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-62566_rabbit-monoclonal-app-antibody-6e10-chimeric-immunohistochemistry-frozen-672023103423..jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining in human skin and skeletal muscle tissues . Corresponding CD44 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-84578-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining in human skin and skeletal muscle tissues . Corresponding CD44 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP1-84578] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84578-img0022.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human lymph node shows strong positivity in plasma membrane in non-germinal center cells.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-84578-img0023.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-84578-img0018.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human squamous cell lung carcinoma shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-84578-img0019.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-84578-img0021.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells. Western blot of ORC2 in HBEC and 293T cell lines. Ponceau S staining of total protein or immunoblot of Chk1 show equal loading of the pairs of lanes. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-464-img0034.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells. Western blot of ORC2 in HBEC and 293T cell lines. Ponceau S staining of total protein or immunoblot of Chk1 show equal loading of the pairs of lanes. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-464] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Chk1-Antibody-Immunohistochemistry-Paraffin-NB100-464-img0029.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-464] - Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-464-img0032.jpg)
![Immunoprecipitation: Chk1 Antibody [NB100-464] - Detection of human Chk1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 (lot 2) used for IP at 6 ug/mg lysate. Chk1 was also immunoprecipitated by a previous lot (lot 1) of this antibody. For blotting immunoprecipitated Chk1, NB100-464 (lot 2) was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Chk1-Antibody-Immunoprecipitation-NB100-464-img0033.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells.(G) WB for indicated proteins in clones indicated on the top. Darker exposure of ORC2 blots is shown at the bottom. Ponceau S stains all proteins on the blot & also indicates equal loading of lanes. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-464_rabbit-polyclonal-chk1-antibody-310202415371972.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC1 in HCT116 p53-/- cells.(A) Strategy for insertion of a blasticidin gene & poly A site after first methionine of ORC1 in the second exon. (B) PCR on genomic DNA of indicated clones. WT: HCT116 p53-/- & ORC1+/+. ORC1 Knockout clones, B14, B48 & BP32 have an insert on both alleles of ORC1 as indicated by the absence of 0.6 kb PCR product. (C) Western blot for indicated proteins in clones indicated on the top. Darker exposure of the ORC1 blots is shown at the bottom. Ponceau S stains all proteins on the blot & also indicates equal loading of lanes. (D) Immunoblot of soluble & chromatin-associated proteins in the clones indicated at the top. For each panel, all the lanes are from the same blot & exposure. (E) Input cell lysate & immunoprecipitates of ORC1 immunoblotted for ORC1. Darker exposure of the top blots is shown in the middle. Tubulin in the cell lysate or the IgG band in the immunoprecipitate serves as loading control.DOI://dx.doi.org/10.7554/eLife.19084.005 Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-464_rabbit-polyclonal-chk1-antibody-310202415525253.jpg)