| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-19127SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82512-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-61640-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-81927-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB100-61100-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97485-0.2mg | |||
| BSA Free | NBP1-97485-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76732-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82245-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76910-0.025mg | |||
![Western Blot: ATG13 [p Ser355] Antibody [NBP2-19127] - Antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13. In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blocking Buffer and probed with the primary antibody at 1ug/mL overnight at 4C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer for 45 minutes at room temperature.](http://images.novusbio.com/fullsize/ATG13-[p-Ser318]-Antibody-Western-Blot-NBP2-19127-img0003.jpg "Western Blot: ATG13 [p Ser355] Antibody [NBP2-19127] - Antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13. In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blocking Buffer and probed with the primary antibody at 1ug/mL overnight at 4C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer for 45 minutes at room temperature.")
![Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining in human cerebral cortex and skeletal muscle tissues . Corresponding WASL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Immunohistochemistry-Paraffin-NBP1-82512-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining in human cerebral cortex and skeletal muscle tissues . Corresponding WASL RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: N-WASP Antibody [NBP1-82512] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Western-Blot-NBP1-82512-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: N-WASP Antibody [NBP1-82512] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82512-img0010.jpg)
![Western Blot: N-WASP Antibody [NBP1-82512] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Western-Blot-NBP1-82512-img0008.jpg)
![Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human cerebral cortex shows moderate to strong positivity in neuronal cells.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Immunohistochemistry-Paraffin-NBP1-82512-img0011.jpg)
![Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human kidney shows moderate to strong positivity in cells in tubules and glomeruli.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Immunohistochemistry-Paraffin-NBP1-82512-img0012.jpg)
![Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human skeletal muscle shows weak positivity in myocytes.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Immunohistochemistry-Paraffin-NBP1-82512-img0013.jpg)
![Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human stomach shows moderate to strong positivity in glandular cells.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Immunohistochemistry-Paraffin-NBP1-82512-img0014.jpg)
![Simple Western: N-WASP Antibody [NBP1-82512] - Simple Western lane view shows a specific band for N-WASP in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/N-WASP-Antibody-Simple-Western-NBP1-82512-img0006.jpg)
![Western Blot: N-WASP Antibody [NBP1-82512] - Generation of nWASP knockdown cell lines. A-549 & SK-MES-1 cells were treated with nWASP siRNA/non-targeting siRNA (NT) at 0.5 μg/ml & then analysed for nWASP expression after 48 h. a QPCR analysis of nWASP transcript expression demonstrates a significant decrease in expression in siRNA treated cells, n = 4 replicates. b PCR also demonstrates a decrease in nWASP expression. c Quantitative analysis of Western blots (n = 4) shows significant decrease in nWASP protein expression in both A-549 & SK-MES1 cell lines after 48 h siRNA treatment. d Representative image showing knockdown of nWASP at protein level in siRNA treated cells at 48 h Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28351346), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82512_rabbit-polyclonal-n-wasp-antibody-310202415392573.jpg)
![Western Blot: N-WASP Antibody [NBP1-82512] - nWASP activity affects A-549 & SK-MES-1 cell growth. a, b nWASP inhibition using 10 μM wiskostatin treatment significantly impairs the growth of A-549 & SK-MES-1 cells, respectively. c, d A significant effect on growth is also evident after 3 days in nWASP knockdown A-549 & SK-MES-1 cells, respectively, when compared to non-targeting control treated cells Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28351346), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82512_rabbit-polyclonal-n-wasp-antibody-310202415304216.jpg)
![Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Western-Blot-NB110-61640-img0040.jpg "Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.")
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (H9010) [NB110-61640] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunocytochemistry-Immunofluorescence-NB110-61640-img0034.jpg)
![Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunohistochemistry-NB110-61640-img0044.jpg)
![Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. 15 ug protein each lane. Block: 1.5% BSA for 30 minutes at RT. Primary antibody at 1:1000 for 2 hours at RT. Secondary antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Western-Blot-NB110-61640-img0031.jpg)
![Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Western-Blot-NB110-61640-img0039.jpg)
![Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunohistochemistry-NB110-61640-img0041.jpg)
![Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunohistochemistry-NB110-61640-img0042.jpg)
![Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunohistochemistry-NB110-61640-img0043.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunohistochemical staining in human lung cancer formalin fixed paraffin-embedded tissue section.](http://images.novusbio.com/fullsize/nb100-81927_rabbit-polyclonal-foxo1-fkhr-p-ser256-antibody-2342024155517.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunohistochemical staining in human lung cancer formalin fixed paraffin-embedded tissue section.")
![Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunofluorescent staining in MCF7 cells.](http://images.novusbio.com/fullsize/nb100-81927_rabbit-polyclonal-foxo1-fkhr-p-ser256-antibody-234202415232510.jpg)
![Western Blot: Aquaporin-2 [p Ser261] Antibody [NB100-61100] - Rat kidney lysate showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser261. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown).](http://images.novusbio.com/fullsize/Aquaporin-2-[p-Ser261]-Antibody-Western-Blot-NB100-61100-img0001.jpg "Western Blot: Aquaporin-2 [p Ser261] Antibody [NB100-61100] - Rat kidney lysate showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser261. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown).")
![Western Blot: Calnexin Antibody [NBP1-97485] - Analysis: Lane 1: HeLa (heat shocked), Lane 2: Vero (heat shocked), Lane 3: Rat-2 (heat shocked), Lane 4: L-929 (heat shocked).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NBP1-97485-img0003.jpg "Western Blot: Calnexin Antibody [NBP1-97485] - Analysis: Lane 1: HeLa (heat shocked), Lane 2: Vero (heat shocked), Lane 3: Rat-2 (heat shocked), Lane 4: L-929 (heat shocked).")
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-97485] - Analysis of human spleen tissue stained with Calnexin, pAb at 10ug/ml.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-97485-img0002.jpg)
![Western Blot: SMC1 [p Ser966] Antibody [NB100-206] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes. For detection of total SMC1, rabbit anti-SMC1 antibody was used.](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Western-Blot-NB100-206-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMC1 [p Ser966] Antibody [NB100-206] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes. For detection of total SMC1, rabbit anti-SMC1 antibody was used.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SMC1 [p Ser966] Antibody [NB100-206] - Neocarzinostatin treated asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight (R) 594 conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-206-img0005.jpg)
![Immunohistochemistry-Paraffin: SMC1 [p Ser966] Antibody [NB100-206] - Human breast carcinoma. Antibody: Affinity purified rabbit antiPhospho SMC1 (S966) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Immunohistochemistry-Paraffin-NB100-206-img0008.jpg)
![Immunohistochemistry-Paraffin: SMC1 [p Ser966] Antibody [NB100-206] - Human testicular seminoma. Antibody: Affinity purified rabbit anti- Phospho SMC1 (S966) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-Immunohistochemistry-Paraffin-NB100-206-img0007.jpg)
![<b>Biological Strategies Validation. </b>SMC1 [p Ser966] Antibody [NB100-206] - Detection of human Phospho SMC1 (S966) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho SMC1 (S966) antibody NB100-206 used for IP at 3 ul per reaction. Phospho SMC1 (S966) was also immunoprecipitated by rabbit anti-SMC1 antibody NB100-204. For blotting immunoprecipitated Phospho SMC1 NB100-206 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. For detection of total SMC1, rabbit anti-SMC1 antibody NB100-204 was used.](http://images.novusbio.com/fullsize/SMC1-[p-Ser966]-Antibody-NB100-206-img0009.jpg)
![Western Blot: RIG-I Antibody [NBP1-76732] - Western blot analysis of RIG-I in Lane 1) A20 cell lysate and Lane 2) Rat heart tissue lysate with RIG-I antibody [NBP1-76732] at 2 ug/mL.](http://images.novusbio.com/fullsize/RIG-I-Antibody-Western-Blot-NBP1-76732-img0004.jpg "Western Blot: RIG-I Antibody [NBP1-76732] - Western blot analysis of RIG-I in Lane 1) A20 cell lysate and Lane 2) Rat heart tissue lysate with RIG-I antibody [NBP1-76732] at 2 ug/mL.")
![Immunohistochemistry: RIG-I Antibody [NBP1-76732] - Immunohistochemistry of RIG-I in mouse testis tissue with RIG-I Antibody NBP1-76732 at 1 ug/mL.](http://images.novusbio.com/fullsize/RIG-I-Antibody-Immunohistochemistry-NBP1-76732-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] - Immunofluorescence of RIG-I in rat testis tissue with RIG-I Antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76732_rabbit-polyclonal-rig-i-antibody-271120242014197.gif)
![Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] - Immunofluorescence of RIG-I in mouse testis tissue with RIG-I Antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76732_rabbit-polyclonal-rig-i-antibody-2711202419592046.gif)
![Immunohistochemistry: RIG-I Antibody - BSA Free [NBP1-76732] - Immunohistochemistry of RIG-I in rat heart tissue with RIG-I Antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76732_rabbit-polyclonal-rig-i-antibody-271120242042539.gif)
![Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] - Immunofluorescence of RIG-I in human testis tissue with RIG-I Antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76732_rabbit-polyclonal-rig-i-antibody-2711202419592068.gif)
![Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] - Immunofluorescence of RIG-I in MCF7 cells with RIG-I Antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76732_rabbit-polyclonal-rig-i-antibody-271120241946344.gif)
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)
![Tau-[p-Thr181]-Antibody-Western-Blot-NB100-82245-img0006.jpg](http://images.novusbio.com/fullsize/Tau-[p-Thr181]-Antibody-Western-Blot-NB100-82245-img0006.jpg "<b>Genetic Strategies Validation. </b>Tau-[p-Thr181]-Antibody-Western-Blot-NB100-82245-img0006.jpg")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Tau [p Thr181] Antibody [NB100-82245] - analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer's Disease using Tau (phospho-Thr181) antibody.](http://images.novusbio.com/fullsize/Tau-[p-Thr181]-Antibody-Immunohistochemistry-Paraffin-NB100-82245-img0005.jpg)
![Western Blot: Tau [p Thr181] Antibody [NB100-82245] - Analysis of extracts from mouse brain tissue using Tau(Phospho-Thr181) Antibody and the same antibody preincubated with blocking peptide.](http://images.novusbio.com/fullsize/Tau-[p-Thr181]-Antibody-Western-Blot-NB100-82245-img0001.jpg)
![Simple Western: Tau [p Thr181] Antibody [NB100-82245] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.](http://images.novusbio.com/fullsize/Tau-[p-Thr181]-Antibody-Simple-Western-NB100-82245-img0004.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-464] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Chk1-Antibody-Immunohistochemistry-Paraffin-NB100-464-img0029.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-464] - Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-464-img0032.jpg)
![Immunoprecipitation: Chk1 Antibody [NB100-464] - Detection of human Chk1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 (lot 2) used for IP at 6 ug/mg lysate. Chk1 was also immunoprecipitated by a previous lot (lot 1) of this antibody. For blotting immunoprecipitated Chk1, NB100-464 (lot 2) was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Chk1-Antibody-Immunoprecipitation-NB100-464-img0033.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells.(G) WB for indicated proteins in clones indicated on the top. Darker exposure of ORC2 blots is shown at the bottom. Ponceau S stains all proteins on the blot & also indicates equal loading of lanes. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-464_rabbit-polyclonal-chk1-antibody-310202415371972.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC1 in HCT116 p53-/- cells.(A) Strategy for insertion of a blasticidin gene & poly A site after first methionine of ORC1 in the second exon. (B) PCR on genomic DNA of indicated clones. WT: HCT116 p53-/- & ORC1+/+. ORC1 Knockout clones, B14, B48 & BP32 have an insert on both alleles of ORC1 as indicated by the absence of 0.6 kb PCR product. (C) Western blot for indicated proteins in clones indicated on the top. Darker exposure of the ORC1 blots is shown at the bottom. Ponceau S stains all proteins on the blot & also indicates equal loading of lanes. (D) Immunoblot of soluble & chromatin-associated proteins in the clones indicated at the top. For each panel, all the lanes are from the same blot & exposure. (E) Input cell lysate & immunoprecipitates of ORC1 immunoblotted for ORC1. Darker exposure of the top blots is shown in the middle. Tubulin in the cell lysate or the IgG band in the immunoprecipitate serves as loading control.DOI://dx.doi.org/10.7554/eLife.19084.005 Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-464_rabbit-polyclonal-chk1-antibody-310202415525253.jpg)
![Western Blot: APP Antibody [NBP1-76910] - Analysis of APP in mouse brain tissue lysate with APP antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/APP-Antibody-Western-Blot-NBP1-76910-img0012.jpg "Western Blot: APP Antibody [NBP1-76910] - Analysis of APP in mouse brain tissue lysate with APP antibody at (A) 0.5 and (B) 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: APP Antibody - BSA Free [NBP1-76910] - Immunofluorescence of APP in Rat Brain cells with APP antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-2711202419451253.jpg)
![Immunocytochemistry/ Immunofluorescence: APP Antibody - BSA Free [NBP1-76910] - Immunofluorescence of APP in mouse brain tissue with APP Antibody at 20 ug/mL. <br><br>Green: APP antibody <br> Red: Phylloidin staining<br> Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-2711202419541571.gif)
![Immunohistochemistry: APP Antibody - BSA Free [NBP1-76910] - Immunohistochemistry of APP in mouse brain tissue with APP Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-2711202419251561.gif)
![Immunohistochemistry: APP Antibody - BSA Free [NBP1-76910] - Immunohistochemical staining of rat brain using APP antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76910_rabbit-polyclonal-app-antibody-imgenex-img-191-271120241932669.jpg)