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Home » FOXO3 » FOXO3 Antibodies » FOXO3 Antibody

FOXO3 Antibody

 (7 Publications)
 (3 Reviews)
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Western Blot: FOXO3 Antibody [NB100-614] - Expression of FOXO increases in aging brain. Protein expression of Foxo1, Foxo3, and phospho-T24/32 Foxo1/3 in young (<3-month, n = 6), adult (3-18-month, n = 6), and old (18-20-month, n = 6) FVB/B6 mixed-strain mouse cerebellums is shown. Each dot represents individual animal. Error bars, mean +/- SEM. *p < .05; **p < .01; ***p < .005. Statistical significance was determined by unpaired t-test. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12701), licensed under a CC-BY licence.
Western Blot: FOXO3 Antibody [NB100-614] - Expression of FOXO increases in aging brain. Protein expression of Foxo1, Foxo3, and phospho-T24/32 Foxo1/3 in young (<3-month, n = 6), adult (3-18-month, n = 6), and old ...read more
Immunocytochemistry/ Immunofluorescence: FOXO3 Antibody [NB100-614] - Analysis of FOXO3 in human B cell lymphoma using anti-FOXO3 antibody .Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: FOXO3 Antibody [NB100-614] - Sample: FFPE section of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-FOXO3a used at a ...read more
Biological Strategies: Western Blot: FOXO3 Antibody [NB100-614] - Detection of Human and Mouse FOXO3a by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 ...read more
Western Blot: FOXO3 Antibody [NB100-614] - FOXO3 Protein expression levels both control group and exercise group for skeletal muscles. Image submitted by a verified customer review *{Mouse WB}*
Independent Antibodies: Immunoprecipitation: FOXO3 Antibody [NB100-614] - Detection of human FOXO3a by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MCF-7 ...read more
Genetic Strategies: Knockout Validated: FOXO3 Antibody [NB100-614] - Expression of FOXO increases in aging brain. Representative Western blotting results. Foxo1- or Foxo1/3-specific knockouts (1KO or 1/3KO) ...read more

Product Details

Summary
Reactivity Hu, Mu, PoSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

  

Genetic Strategies

    

Order Details

100% Guarantee on every product

FOXO3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 625 and the C-terminus (residue 673) of human Forkhead Box O3a using the numbering given in Swiss-Prot entry O43524 (GeneID 2309).
Specificity
NB100-614 is specific for human FOXO3a protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXO3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:250 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1:2000 - 1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. FOXO3 antibody validated for ICC/IF, WB from verified customer reviews.
Theoretical MW
71.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
FOXO3 Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB100-614 in the following applications:
Publications
Read Publications using
NB100-614 in the following applications:

  • ICC/IF
    1 publication
  • IHC
    1 publication
  • KD
    1 publication
  • WB
    4 publications

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID:33147486)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FOXO3 Antibody

  • AF6q21 protein
  • AF6q21
  • DKFZp781A0677
  • FKHRL1
  • FKHRL1MGC31925
  • FKHRL1P2
  • forkhead box O3
  • forkhead box protein O3
  • forkhead homolog (rhabdomyosarcoma) like 1
  • Forkhead in rhabdomyosarcoma-like 1
  • forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
  • FOXO2
  • FoxO3
  • FOXO3A
  • FOXO3Aforkhead box O3A
  • MGC12739

Background

The FOXO3 gene is known to promote longevity in animals and has now been discovered to encode a tumor suppressor. The new gene, which was inactivated in two-thirds of patients studied, presents a potent new target for breast cancer. The protein, named Forkhead box class O3 (FOXO3), is associated with better outcomes for breast cancer patients. Conversely, if FOXO3 was inactivated, patients had worse outcomes. FOXO3 is inactivated in many cancer patients by an enzyme called IKK, which is an oncoprotein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100&amp;nbsp;ng/mL Human&amp;nbsp;PDGF&amp;nbsp;(Catalog&amp;nbsp;# 120‑HD) for 15&amp;nbsp;minutes and MCF‑7 human breast cancer cell line untreated or treated with 100&amp;nbsp;ng/mL&amp;nbsp;Recombinant Human IGF-1 (Catalog&amp;nbsp;# 291‑G1) for 15&amp;nbsp;minutes. PVDF membrane was probed with 0.5&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog&amp;nbsp;# HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group&amp;nbsp;1.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog&amp;nbsp;# 291-G1) for 15&amp;nbsp;minutes, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60&amp;nbsp;kDa (as indicated) using 5&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887). This experiment was conducted&amp;nbsp;under&amp;nbsp;reducing&amp;nbsp;conditions and using the 12-230 kDa separation system.
Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with&amp;nbsp;100&amp;nbsp;ng/mL Recombinant human IGF-1 (Catalog&amp;nbsp;# 291-G1, dark orange filled histogram) for 15&amp;nbsp;minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887) or control antibody (Catalog&amp;nbsp;# AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog&amp;nbsp;# F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
(26 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
FoxO1/FKHR
Antibody (83N7F8) - B
NBP2-31376
Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on U2-OS cells with FoxO1/FKHR [83N7F8] Antibody NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on RH-30 cells with FoxO1/FKHR Antibody [83N7F8] NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
(1 Review)
(5 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
Bim Antibody - BSA
Free
NBP1-76963
Immunocytochemistry/Immunofluorescence: Bim Antibody [NBP1-76963] - K562 cells with at 20 ug/mL.
Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Rat Myeloma Cell line:Loading: 15 ug of rat myeloma YB2/0 cell lysate per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.
Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in HeLa Cells. Loading: 15 ug of lysate per lane. Antibodies: Bim NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate. Bim antibody can detect all three human isoforms, including EL, L and S isoforms.
(7 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, Simple Western, WB
PIK3CA Antibody
NBP2-19804
Western Blot: PIK3CA Antibody [NBP2-19804] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP2-19804] - Human Breast Cancer, using PI3 kinase p110 alpha antibody. Antigen Retrieval: Trilogy&amp;#8482; (EDTA based, pH 8.0) buffer, 15min.
Western Blot: PIK3CA Antibody [NBP2-19804] - PIK3CA Antibody Sample (50 ug of whole cell lysate) A: C2C12 B: C2C12+AEA10mM NBP2-19804 diluted at 1:1000.
(3 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
PI 3-Kinase p110
delta Antibody (OTI1D ...
NBP2-46404
Immunohistochemistry: PI 3-Kinase p110 delta Antibody (1D11) [NBP2-46404] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PI 3-Kinase p110 delta Antibody (1D11) [NBP2-46404] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PI 3-Kinase p110 delta Antibody (1D11) [NBP2-46404] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
(1 Review)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P
PI 3-Kinase p110
beta/PIK3CB Antibody
NBP1-33116
Immunocytochemistry/Immunofluorescence: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Sample (50 ug of whole cell lysate) A: C2C12 B: C2C12+AEA10mM antibody diluted at 1:1000.
Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE; antibody diluted at 1:1000.
(2 Publications)
Species: Bv, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
PI 3-Kinase p110
gamma Antibody
NBP2-15071
Western Blot: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Human Breast Cancer, using PI3K gamma antibody. Antigen Retrieval: Trilogy&amp;#8482; (EDTA based, pH 8.0) buffer, 15min
Western Blot: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Whole cell extract (30ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term diluted at 1:1000.
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
PYK2/FAK2 Antibody
(SC06-15)
NBP2-67627
Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.
Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.
Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.
(1 Review)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
FOXO4 Antibody
(JJ09-11)
NBP2-67892
Flow Cytometry: FOXO4 Antibody (JJ09-11) [NBP2-67892] - Analysis of 293 cells with FOXO4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Immunocytochemistry/Immunofluorescence: FOXO4 Antibody (JJ09-11) [NBP2-67892] - Staining FOXO4 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: FOXO4 Antibody (JJ09-11) [NBP2-67892] - Staining FOXO4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
p27/Kip1 [p
Thr157] Antibody
AF1555
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog&amp;nbsp;# 291‑G1) for 20 minutes. PVDF membrane was probed with 0.5&amp;nbsp;µg/mL Human Phospho-p27/Kip1 (T157) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1555) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p27/Kip1 (T157) was detected at approximately 27 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Review)
(12 Publications)
Species: Hu
Applications: WB
Sirtuin 1/SIRT1
Antibody (1F3) - BSA ...
NBP1-51641
Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.
Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).
Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
(7 Publications)
Species: Hu, Mu, Pm, Rb, Rt
Applications: ELISA, Flow-IC, Flow, ICC/IF, IHC-FrFl, IHC, IHC-P, WB
Fbx32 Antibody
(JE41-27)
NBP2-76836
Western Blot: Fbx32 Antibody (JE41-27) [NBP2-76836] - Western blot analysis of Fbx32 on mouse skeletal muscle tissue using anti-Fbx32 antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: Fbx32 Antibody (JE41-27) [NBP2-76836] - ICC staining Fbx32 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: Fbx32 Antibody (JE41-27) [NBP2-76836] - ICC staining Fbx32 in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
IGF-I/IGF-1
[Unconjugated]
291-G1
1 μg/lane of Recombinant Human IGF-I/IGF-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at&amp;nbsp;7&amp;nbsp;kDa.
Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.3‑1.5&amp;nbsp;ng/mL.
MALDI-TOF analysis of Recombinant Human IGF-I/IGF-1.The major peak corresponds to the calculated molecular mass, 7650 Da. The minor peak at 7873 is a matrix-associated artifact of the MALDI-TOF.
(8 Reviews)
(173 Publications)
Species: Hu
Applications: BA
TOR/mTOR [p
Ser2448] Antibody
NB600-607
Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Brown arrow= IBa1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and IBA1 positive cells. IHC-P Image submitted by a verified customer review.
Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Rapamycin reduces mTOR phosphorylation induced by TII. Whole-cell lysates were prepared from astrocytes activated with TII for 2 h. 10 nM Rapamycin was added at the beginning of the experiment and equal amounts of protein were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR) (upper gel) and consequently for beta-actin (lower gel). Image collected and cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-1), licensed under a CC-BY licence.
N/A: TOR/mTOR [p Ser2448] Antibody [NB600-607]
(2 Reviews)
(9 Publications)
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, NULL, WB
p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
(15 Publications)
Species: Pm, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Insulin was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10&amp;nbsp;µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(14 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
26S proteasome
subunit 9 Antibody
NBP2-47561
Immunohistochemistry-Paraffin: 26S proteasome subunit 9 Antibody [NBP2-47561] - Staining of human kidney, liver, skin and testis using Anti-PSMD9 antibody NBP2-47561 (A) shows similar protein distribution across tissues to independent antibody NBP2-47560(B).
Immunohistochemistry-Paraffin: 26S proteasome subunit 9 Antibody [NBP2-47561] - Staining of human kidney using Anti-PSMD9 antibody.
Immunohistochemistry-Paraffin: 26S proteasome subunit 9 Antibody [NBP2-47561] - Staining of human liver using Anti-PSMD9 antibody.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
TMED7 Antibody
NBP1-90949
Immunohistochemistry-Paraffin: TMED7 Antibody [NBP1-90949] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Western Blot: TMED7 Antibody [NBP1-90949] - Analysis in human cell line CAPAN-2.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
Dynactin 6 Antibody
NBP1-80616
Immunohistochemistry-Paraffin: Dynactin 6 Antibody [NBP1-80616] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Western Blot: Dynactin 6 Antibody [NBP1-80616] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Western Blot: Dynactin 6 Antibody [NBP1-80616] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
SSSCA1 Antibody
(OTI2F5) - Azide and ...
NBP2-74359
Western Blot: SSSCA1 Antibody (OTI2F5) - Azide and BSA Free [NBP2-74359] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSSCA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSSCA1.
Flow Cytometry: SSSCA1 Antibody (OTI2F5) - Azide and BSA Free [NBP2-74359] - Analysis of Hela cells, using anti-SSSCA1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: SSSCA1 Antibody (OTI2F5) - Azide and BSA Free [NBP2-74359] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SSSCA1 antibody, and then analyzed by flow cytometry.
Species: Hu
Applications: CyTOF-ready, Flow, ICC/IF, IHC, WB

Publications for FOXO3 Antibody (NB100-614)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 4 applications: ICC/IF, IHC, KD, WB.


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ICC/IF
(1)
IHC
(1)
KD
(1)
WB
(4)
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Human
(1)
Mouse
(4)
Porcine
(1)
All Species

Reviews for FOXO3 Antibody (NB100-614) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-614:
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WB
(2)
IF
(1)
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Human
(2)
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot FOXO3 NB100-614
Enlarge 		3
reviewed by:
Insu Kwon
 WB Mouse 02/06/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle homogenate
SpeciesMouse
LotA2
Immunofluorescence FOXO3 NB100-614
Enlarge 		5
reviewed by:
Verified Customer
 IF Human 06/04/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedHuman B cell lymphoma
SpeciesHuman

Blocking

Blocking Detailsno blocking

Primary Anitbody

Dilution Ratio1:200 in 3% BSA in PBS for overnight at 4 degree

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit alexa 488
Secondary Manufacturer Cat#A-21206
Secondary Concentration1:1,000

Details

Detection NotesPicture was taken on EVOS AUTO FL using 488 excitation.
Fixation DetailsFixed with 4% paraformaldehyde for 15 min then permeabilized with 0.2% TX100 in PBS for 5 min under ambient temperature
Wash Description3 times 5 min/each PBS wash
Western Blot FOXO3 NB100-614
Enlarge 		5
reviewed by:
Verified Customer
 WB Human 06/04/2016
View

Summary

ApplicationWestern Blot
Sample Testedhuman brain lysate
SpeciesHuman

Blocking

Blocking Details5% non fat dried milk in TBS-T

Primary Anitbody

Dilution Ratio1:1,000 in 3% BSA in TBS, 4 degree overnight incubation

Secondary Antibody

Secondary DescriptionGoat anti rabbit-HRP
Secondary Manufacturer Cat#31460
Secondary Concentration1:10,000

Details

Detection NotesBlot was incubated with ECL-femto (Pierce) for 2 min. X-ray film was overlaid in dark and processed through Kodak x-omat.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FOXO3 Antibody (NB100-614). (Showing 1 - 1 of 1 FAQs).

  1. I've bought 2 antibodies from novus biologicals (NB100-614, NB100-2312) and now i am optimizing them for IP. Which beads did you use, protein a or protein g beads? in some cases only one kind of beads work, i have now tried protein a beads. If possible it would be also very helpful which protocol you used for the IP? (buffers, volumes, incubation times?)
    • Protein A beads were used for IP for these products. We don't have a specific protocol. We recommend for NB100-2312 a dilution of 1 to 10 mcg/mg lysate. For NB100-614 the dilution is 2 to 5 ug/mg lysate. Please see our general IP protocol.

Control Lysate(s)

FOXO3 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for FOXO3 Antibody (NB100-614)

Discover related pathways, diseases and genes to FOXO3 Antibody (NB100-614). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for FOXO3 Antibody (NB100-614)

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Pathways for FOXO3 Antibody (NB100-614)

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Recent Reviews

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Western Blot FOXO3 NB100-614
Enlarge
 Insu Kwon
02/06/2017
Application: WB
Species: Mouse
read this review
Immunofluorescence FOXO3 NB100-614
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 Verified Customer
06/04/2016
Application: IF
Species: Human
read this review
Western Blot FOXO3 NB100-614
Enlarge
 Verified Customer
06/04/2016
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol FOXO3
Entrez
Uniprot