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FOXO3 Antibody

Images

 
Western Blot: FOXO3 Antibody [NB100-614] - Expression of FOXO increases in aging brain. Protein expression of Foxo1, Foxo3, and phospho-T24/32 Foxo1/3 in young (<3-month, n = 6), adult (3-18-month, n = 6), and old ...read more
Immunocytochemistry/ Immunofluorescence: FOXO3 Antibody [NB100-614] - Analysis of FOXO3 in human B cell lymphoma using anti-FOXO3 antibody .Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: FOXO3 Antibody [NB100-614] - Sample: FFPE section of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-FOXO3a used at a ...read more
Biological Strategies: Western Blot: FOXO3 Antibody [NB100-614] - Detection of Human and Mouse FOXO3a by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 ...read more
Western Blot: FOXO3 Antibody [NB100-614] - FOXO3 Protein expression levels both control group and exercise group for skeletal muscles. Image submitted by a verified customer review *{Mouse WB}*
Independent Antibodies: Immunoprecipitation: FOXO3 Antibody [NB100-614] - Detection of human FOXO3a by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MCF-7 ...read more
Genetic Strategies: Knockout Validated: FOXO3 Antibody [NB100-614] - Expression of FOXO increases in aging brain. Representative Western blotting results. Foxo1- or Foxo1/3-specific knockouts (1KO or 1/3KO) ...read more

Product Details

Summary
Reactivity Hu, Mu, PoSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

FOXO3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 625 and the C-terminus (residue 673) of human Forkhead Box O3a using the numbering given in Swiss-Prot entry O43524 (GeneID 2309).
Specificity
NB100-614 is specific for human FOXO3a protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXO3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:250 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1:2000 - 1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. FOXO3 antibody validated for ICC/IF, WB from verified customer reviews.
Theoretical MW
71.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
FOXO3 Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB100-614 in the following applications:

Publications
Read Publications using
NB100-614 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • KD
    1 publication
  • WB
    4 publications

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID:33147486)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FOXO3 Antibody

  • AF6q21 protein
  • AF6q21
  • DKFZp781A0677
  • FKHRL1
  • FKHRL1MGC31925
  • FKHRL1P2
  • forkhead box O3
  • forkhead box protein O3
  • forkhead homolog (rhabdomyosarcoma) like 1
  • Forkhead in rhabdomyosarcoma-like 1
  • forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
  • FOXO2
  • FoxO3
  • FOXO3A
  • FOXO3Aforkhead box O3A
  • MGC12739

Background

The FOXO3 gene is known to promote longevity in animals and has now been discovered to encode a tumor suppressor. The new gene, which was inactivated in two-thirds of patients studied, presents a potent new target for breast cancer. The protein, named Forkhead box class O3 (FOXO3), is associated with better outcomes for breast cancer patients. Conversely, if FOXO3 was inactivated, patients had worse outcomes. FOXO3 is inactivated in many cancer patients by an enzyme called IKK, which is an oncoprotein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FOXO3 Antibody (NB100-614)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 4 applications: ICC/IF, IHC, KD, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
KD
(1)
WB
(4)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
Porcine
(1)
All Species

Reviews for FOXO3 Antibody (NB100-614) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-614:
Filter by Applications
WB
(2)
IF
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot FOXO3 NB100-614
Enlarge
3
reviewed by:
Insu Kwon
WB Mouse 02/06/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle homogenate
SpeciesMouse
LotA2
Immunofluorescence FOXO3 NB100-614
Enlarge
5
reviewed by:
Verified Customer
IF Human 06/04/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedHuman B cell lymphoma
SpeciesHuman

Blocking

Blocking Detailsno blocking

Primary Anitbody

Dilution Ratio1:200 in 3% BSA in PBS for overnight at 4 degree

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit alexa 488
Secondary Manufacturer Cat#A-21206
Secondary Concentration1:1,000

Details

Detection NotesPicture was taken on EVOS AUTO FL using 488 excitation.
Fixation DetailsFixed with 4% paraformaldehyde for 15 min then permeabilized with 0.2% TX100 in PBS for 5 min under ambient temperature
Wash Description3 times 5 min/each PBS wash
Western Blot FOXO3 NB100-614
Enlarge
5
reviewed by:
Verified Customer
WB Human 06/04/2016
View

Summary

ApplicationWestern Blot
Sample Testedhuman brain lysate
SpeciesHuman

Blocking

Blocking Details5% non fat dried milk in TBS-T

Primary Anitbody

Dilution Ratio1:1,000 in 3% BSA in TBS, 4 degree overnight incubation

Secondary Antibody

Secondary DescriptionGoat anti rabbit-HRP
Secondary Manufacturer Cat#31460
Secondary Concentration1:10,000

Details

Detection NotesBlot was incubated with ECL-femto (Pierce) for 2 min. X-ray film was overlaid in dark and processed through Kodak x-omat.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FOXO3 Antibody (NB100-614). (Showing 1 - 1 of 1 FAQs).

  1. I've bought 2 antibodies from novus biologicals (NB100-614, NB100-2312) and now i am optimizing them for IP. Which beads did you use, protein a or protein g beads? in some cases only one kind of beads work, i have now tried protein a beads. If possible it would be also very helpful which protocol you used for the IP? (buffers, volumes, incubation times?)
    • Protein A beads were used for IP for these products. We don't have a specific protocol. We recommend for NB100-2312 a dilution of 1 to 10 mcg/mg lysate. For NB100-614 the dilution is 2 to 5 ug/mg lysate. Please see our general IP protocol.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for FOXO3 Antibody (NB100-614)

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Blogs on FOXO3

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Contact Information

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Product PDFs

Calculators

Concentration Calculator

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Recent Reviews

4.3
5
2
4
0
3
1
2
0
1
0

Insu Kwon
02/06/2017
Application: WB
Species: Mouse

Verified Customer
06/04/2016
Application: IF
Species: Human

Verified Customer
06/04/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol FOXO3
Entrez
Uniprot