Western Blot: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Western Blot analysis of Mouse Ventricle lysates showing detection of CaMKII alpha/beta protein using Mouse Anti-CaMKII alpha/beta ...read more
Immunocytochemistry/ Immunofluorescence: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Immunocytochemistry/ Immunofluorescence analysis using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, ...read more
Immunohistochemistry: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Immunohistochemistry analysis using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, Clone 22B1 (NB110-96869). Tissue: colon ...read more
Immunohistochemistry: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Immunohistochemistry analysis using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, Clone 22B1 (NB110-96869). Tissue: ...read more
1 ug/ml was sufficient for detection of 0.2 ug CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.Use in immunoblotting reported in scientific literature (PMID: 27599291).
Read Publications using NB110-96869 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.4), 50% Glycerol
0.09% Sodium Azide
Protein G purified
Alternate Names for CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1)
calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
calcium/calmodulin-dependent protein kinase II alpha
calcium/calmodulin-dependent protein kinase II alpha-B subunit
CaM kinase II alpha subunit
CaM kinase II subunit alpha
CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha
CaMK-II alpha subunit
CaMK-II subunit alpha
CaM-kinase II alpha chain
KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain
CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho- CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Auto-phosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) and receive a gift card or discount.