CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1)

Images

 
Western Blot: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Western Blot analysis of Mouse Ventricle lysates showing detection of CaMKII alpha/beta protein using Mouse Anti-CaMKII alpha/beta ...read more
Immunocytochemistry/ Immunofluorescence: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Immunocytochemistry/ Immunofluorescence analysis using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, ...read more
Immunohistochemistry: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Immunohistochemistry analysis using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, Clone 22B1 (NB110-96869). Tissue: colon ...read more
Immunohistochemistry: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Immunohistochemistry analysis using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, Clone 22B1 (NB110-96869). Tissue: ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP
Clone
22B1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) Summary

Immunogen
Synthetic peptide
Modification
p Thr286, p Thr287
Localization
Cytoplasm , Mitochondrion , Nucleus , Cell Junction , Synapse , Presynaptic Cell Membrane
Specificity
This antibody is specific for a and B subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in B).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CAMK2A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • Western Blot 1:1000
Application Notes
1 ug/ml was sufficient for detection of 0.2 ug CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.Use in immunoblotting reported in scientific literature (PMID: 27599291).
Publications
Read Publications using
NB110-96869 in the following applications:

  • IB
    1 publication
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1)

  • calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
  • calcium/calmodulin-dependent protein kinase II alpha
  • calcium/calmodulin-dependent protein kinase II alpha-B subunit
  • CaM kinase II alpha subunit
  • CaM kinase II subunit alpha
  • CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha
  • CaMK-II alpha subunit
  • CaMK-II subunit alpha
  • CaMKIINalpha
  • CaM-kinase II alpha chain
  • EC 2.7.11
  • EC 2.7.11.17
  • KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain

Background

CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho- CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Auto-phosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CaMKII alpha/beta Antibody (NB110-96869)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IB, WB.


Filter By Application
IB
(1)
WB
(5)
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Mouse
(4)
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Showing Publications 1 - 7 of 7.
Publications using NB110-96869 Applications Species
Kim Y, Jang E, Lee Y et al. Miniature Transparent Dopamine Sensor Based on Nanosphere Lithography Advanced Materials Technologies 2023-04-02 (WB) WB
Ghane MA, Wei W, Yakout DW et al. Arc ubiquitination regulates endoplasmic reticulum-mediated Ca2+ release and CaMKII signaling Frontiers in cellular neuroscience 2023-03-14 [PMID: 36998269] (WB, Mouse) WB Mouse
Lee Y, Kang JS, Ham OJ et al. Gut metabolite trimethylamine N-oxide induces aging-associated phenotype of midbrain organoids for the induced pluripotent stem cell-based modeling of late-onset disease Frontiers in Aging Neuroscience 2022-08-16 [PMID: 36051303]
Cai B, Kasikara C, Doran AC et al. MerTK signaling in macrophages promotes the synthesis of inflammation resolution mediators by suppressing CaMKII activity. Sci Signal 2018-09-25 [PMID: 30254055] (WB, Mouse) WB Mouse
Ersoy BA, Maner-Smith KM, Li Y et al. Thioesterase-mediated control of cellular calcium homeostasis enables hepatic ER stress. J. Clin. Invest. 2017-11-20 [PMID: 29202465] (WB, Mouse) WB Mouse
Conlon DM, Thomas T, Fedotova T et al. Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis. J Clin Invest 2016-09-06 [PMID: 27599291] (IB) IB
Jiang L, Xu L, Song Y et al. CaMK II/CREB/Wnt/beta-catenin signaling cascade regulates Angiotensin II-induced podocyte injury and albuminuria. J Biol Chem 2013-06-26 [PMID: 23803607] (WB, Mouse) WB Mouse

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Blogs on CaMKII alpha/beta.

CaMKII stimulates autophagic degradation of 'ID', a new frontier against cancer
By Yoskaly Lazo-Fernandez, PhD The field of Cancer Stem Cell (CSC) research has been gaining traction in recent years1. CSCs are a minority group of cells (usually about 1 in 10000) within solid tumors of hematolog...  Read full blog post.

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Bioinformatics

Gene Symbol CAMK2A
Entrez
Uniprot