ODF2 Antibody (1A1)

Images

 
Western Blot: ODF2 Antibody (1A1) [H00004957-M01] - (Transfected Lysate)
Immunocytochemistry/ Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01] - Analysis of monoclonal antibody to ODF2 on HeLa cell. Antibody concentration 10 ug/ml.

Product Details

Summary
Reactivity Hu, CeSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
1A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ODF2 Antibody (1A1) Summary

Immunogen
ODF2 (NP_002531.3 706 a.a. - 804 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KEHALSKERAAQNKILDLETQLSRTKTELSQLRRSRDDADRRYQSRLQDLKDRLEQSESTNRSMQNYVQFLKSSYANVFGDGPYSTFLTSSPIRSRSPP
Specificity
ODF2 - outer dense fiber of sperm tails 2 (1A1)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ODF2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. ICC/IF usage reported in scientific literature. IHC usage reported in scientific literature (PMID: 24231678).
Reviewed Applications
Read 1 Review rated 5
using
H00004957-M01 in the following applications:

Publications
Read Publications using
H00004957-M01 in the following applications:

  • 4 publications
  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

C. elegans reactivity reported in scientific literature (PMID: 24231678).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ODF2 Antibody (1A1)

  • Cenexin
  • FLJ44866
  • MGC111096
  • MGC9034
  • ODF2/1
  • ODF2/2
  • ODF8484-kD
  • outer dense fiber of sperm tails 2
  • outer dense fiber protein 2
  • sperm tail structural protein

Background

The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of this gene; however, not all isoforms and variants have been fully described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, ICC/IF, IHC

Publications for ODF2 Antibody (H00004957-M01)(7)

We have publications tested in 2 confirmed species: Human, C. elegans.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(4)
IHC
(1)
WB
(1)
All Applications
Filter By Species
Human
(3)
C. elegans
(1)
All Species

Review for ODF2 Antibody (H00004957-M01) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00004957-M01:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
ICC Human 08/25/2010
View

Summary

ApplicationImmunocytochemistry
Sample TestedhTERT RPE-1 cell line from ATCC
SpeciesHuman
CommentsThis ODF2 monoclonal antibody works very well in IF assay. It is a good mother centriole marker.

Blocking

Blocking DetailsBlocking Buffer: 3% BSA, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:200, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: 37 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Alexa Fluor 555 goat anti-mouse IgG2a (?2a), Secondary Ab Dilution: 1:1000
Secondary Manufacturer Cat#Invitrogen A21137

Details

Fixation DetailsSample Preparation: 3d old in growth medium

Comments

CommentsThis ODF2 monoclonal antibody works very well in IF assay. It is a good mother centriole marker.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ODF2 Antibody (H00004957-M01) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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08/25/2010
Application: ICC
Species: Human

Bioinformatics

Gene Symbol ODF2
Entrez
Uniprot