| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1936SS | |||
| Azide and BSA Free | NBP2-80955 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58345SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24587-25ul | |||
| Azide and BSA Free | NBP3-43855 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24588-25ul | |||
| Azide and BSA Free | NBP3-43856 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93653-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84412-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB600-939-0.1mg | |||
| HRP | NB120-7339-0.1mg | |||
![Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - CBs lose their colocalization with SMN nuclear foci in diabetic mice. Normal and pathological distribution of SMN proteins in sensory neurons. SMN proteins were found throughout the cytoplasm and as nuclear foci in control sensory neurons. SMN nuclear foci localized within CBs (coilin) in controls (upper panels, white arrows), whereas in the diabetic nucleus the numerous CBs were present but lost their colocalization with SMN nuclear foci (lower panels, yellow arrows). Arrowheads indicate sensory neurons magnified in the insets. Scale bars: 20 um, 10 um in insets. Image collected and cropped by CiteAb from the following publication (//dmm.biologists.org/lookup/doi/10.1242/dmm.028225), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunohistochemistry-NB100-1936-img0007.jpg "Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - CBs lose their colocalization with SMN nuclear foci in diabetic mice. Normal and pathological distribution of SMN proteins in sensory neurons. SMN proteins were found throughout the cytoplasm and as nuclear foci in control sensory neurons. SMN nuclear foci localized within CBs (coilin) in controls (upper panels, white arrows), whereas in the diabetic nucleus the numerous CBs were present but lost their colocalization with SMN nuclear foci (lower panels, yellow arrows). Arrowheads indicate sensory neurons magnified in the insets. Scale bars: 20 um, 10 um in insets. Image collected and cropped by CiteAb from the following publication (//dmm.biologists.org/lookup/doi/10.1242/dmm.028225), licensed under a CC-BY license.")
![Western Blot: SMN Antibody (2B1) [NB100-1936] - Analysis of SMN expression in 1) HeLa, 2) NTera2, 3) HepG2, 4) MCF7, 5) NIH 3T3, 6) PC12, and 7) COS7 whole cell lysates.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Western-Blot-NB100-1936-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB100-1936 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - scIF of control (f) and etv5b MO (g) cells from pmnx1:eGFP embryos. H2B, GFP and Smn signals are shown in Z-projected confocal sections. GFP+ cells marked by white rectangles are magnified on the right. Scale bars: 10 um for low and 5 um for high magnification. Image collected and cropped by Citeab from the following publication (Transcriptional enhancement of Smn levels in motoneurons is crucial for proper axon morphology in zebrafish. Sci Rep (2016) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with SMN Antibody [2B1] conjugated to Alexa Fluor 488 (NB100-1936AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0012.jpg)
![Flow Cytometry: SMN Antibody (2B1) [NB100-1936] - An intracellular stain was performed on Neuro2a cells with SMN Antibody (2B1) NB100-1936 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Flow-Cytometry-NB100-1936-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - The SMN antibody was tested at a 1:250 dilution in HeLa cells against DyLight 488 (Green). Actin nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0004.jpg)
![Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - Analysis of SMN on mouse brain using NB100-1936.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunohistochemistry-NB100-1936-img0001.jpg)
![Flow Cytometry: SMN Antibody (2B1) [NB100-1936] - An intracellular stain was performed on Ntera2 cells with SMN Antibody (2B1) NB100-1936 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Flow-Cytometry-NB100-1936-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - CWC22 is colocalized with nuclear speckles & upregulated in diabetic DRG sensory neurons. (A) Subcellular distribution of TDP-43 & CWC22 in control DRG sensory neurons. TDP-43 was stained diffusely in the nucleus, excluding SMN foci in sensory neurons. CWC22 consistently colocalized with a marker protein SC35 of nuclear speckles in sensory neurons. No obvious differences in the subcellular localization of CWC22 were identified in diabetic neurons (not shown) compared with controls. Scale bar: 10 μm. (B) qRT-PCR analysis of Cwc22 mRNA expression in diabetic & control mice. Cwc22 expression was upregulated ∼2.5-fold in diabetic DRGs. *P<0.05, unpaired two-tailed Student's t-test. Data represented as mean±s.e.m. See Cheng et al. (2015) for microarray data indicating rises in Cwc22 expression as reported separately. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/dmm/article/10/3/215/2257/Diabetic-polyneuropathy-sensory-neurons-nuclear), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1936_mouse-monoclonal-smn-antibody-2b1-3102024168396.jpg)
![Western Blot: CDR2 Antibody [NB110-58345] - Cdr2 expressed during mitosis, ubiquitinated and degraded during mitotic exit. Upper left, flow cytometry of G1/S- and G2/M-arrested HEK293s. Upper right, western blots of HEK293 G1/S and G2/M extracts probed with anti-cdr2 (NB110; top), cyclinB1 (middle) and gamma-tubulin (bottom). Bottom panel, cdr2 and cyclin B1 protein quantitation, normalized here and in D to gamma--tubulin levels; *p<0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0010045), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CDR2-Antibody-Western-Blot-NB110-58345-img0009.jpg "Western Blot: CDR2 Antibody [NB110-58345] - Cdr2 expressed during mitosis, ubiquitinated and degraded during mitotic exit. Upper left, flow cytometry of G1/S- and G2/M-arrested HEK293s. Upper right, western blots of HEK293 G1/S and G2/M extracts probed with anti-cdr2 (NB110; top), cyclinB1 (middle) and gamma-tubulin (bottom). Bottom panel, cdr2 and cyclin B1 protein quantitation, normalized here and in D to gamma--tubulin levels; *p<0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0010045), licensed under a CC-BY license.")
![Immunohistochemistry: CDR2 Antibody [NB110-58345] - Purkinje neurons of the cerebellum.](http://images.novusbio.com/fullsize/CDR2-Antibody-Immunohistochemistry-NB110-58345-img0004.jpg)
![Flow Cytometry: CDR2 Antibody [NB110-58345] - Analysis using the DyLight 488 conjugate of NB110-58345. Thymus epithelial cells CDR2. Image from verified customer review.](http://images.novusbio.com/fullsize/CDR2-Antibody-Flow-Cytometry-NB110-58345-img0008.jpg)
![Western Blot: CDR2 Antibody [NB110-58345] - Analysis of CDR2 antibody on HeLa whole cell.](http://images.novusbio.com/fullsize/CDR2-Antibody-Western-Blot-NB110-58345-img0006.jpg)
![Western Blot: AHNAK Antibody (3G7) [H00079026-M01] - Analysis of AHNAK expression in transfected 293T cell line by AHNAK monoclonal antibody (M01), clone 3G7.Lane 1: AHNAK transfected lysate(16 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/AHNAK-Antibody-3G7-Western-Blot-H00079026-M01-img0006.jpg "Western Blot: AHNAK Antibody (3G7) [H00079026-M01] - Analysis of AHNAK expression in transfected 293T cell line by AHNAK monoclonal antibody (M01), clone 3G7.Lane 1: AHNAK transfected lysate(16 KDa).Lane 2: Non-transfected lysate.")
![Immunoprecipitation: AHNAK Antibody (3G7) [H00079026-M01] - Transfected lysate using anti-AHNAK monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with AHNAK MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/AHNAK-Antibody-3G7-Immunoprecipitation-H00079026-M01-img0003.jpg)
![Immunoprecipitation: AHNAK Antibody (3G7) [H00079026-M01] - Analysis of AHNAK transfected lysate using anti-AHNAK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AHNAK MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/AHNAK-Antibody-3G7-Immunoprecipitation-H00079026-M01-img0004.jpg)
![ELISA: AHNAK Antibody (3G7) [H00079026-M01] - Detection limit for recombinant GST tagged AHNAK is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AHNAK-Antibody-3G7-ELISA-H00079026-M01-img0007.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Analysis of extracts of HepG2 cells, using Alpha-2-Macroglobulin (alpha 2-Macroglobulin) Rabbit mAb (NBP3-16875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-7V6M10-Western-Blot-NBP3-16875-img0007.jpg "Western Blot: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Analysis of extracts of HepG2 cells, using Alpha-2-Macroglobulin (alpha 2-Macroglobulin) Rabbit mAb (NBP3-16875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Human cervix cancer tissue using Alpha-2-Macroglobulin (A2M) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-immunohistochemistry-paraffin-1110202485424..jpg)
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Human tonsil tissue using Alpha-2-Macroglobulin (A2M) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-immunohistochemistry-paraffin-1110202485548..jpg)
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Mouse brain tissue using Alpha-2-Macroglobulin (A2M) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-immunohistochemistry-paraffin-111020248578..jpg)
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Rat spleen tissue using Alpha-2-Macroglobulin (A2M) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-immunohistochemistry-paraffin-111020248589..jpg)
![Immunocytochemistry/ Immunofluorescence: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Immunofluorescence analysis of paraffin-embedded human liver using Alpha-2-Macroglobulin ) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-5220251136127.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Immunofluorescence analysis of paraffin-embedded mouse liver using Alpha-2-Macroglobulin ) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-52202511365123.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Immunofluorescence analysis of paraffin-embedded rat liver using Alpha-2-Macroglobulin ) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-52202511383776.jpg)
![Immunohistochemistry: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Alpha-2-Macroglobulin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-52202511365143.jpg)
![Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417] - Human lung cancer. MSH3 stained by MSH3 antibody [C2C3], C-term diluted at 1:600. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MSH3-Antibody-Immunohistochemistry-Paraffin-NBP2-19417-img0007.jpg "Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417] - Human lung cancer. MSH3 stained by MSH3 antibody [C2C3], C-term diluted at 1:600. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417] - Human colon carcinoma, using MSH3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MSH3-Antibody-Immunohistochemistry-Paraffin-NBP2-19417-img0002.jpg)
![Western Blot: MSH3 Antibody [NBP2-19417] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MSH3 antibody [C2C3], C-term (NBP2-19417) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19417_rabbit-polyclonal-msh3-antibody-91020242041224.jpg)
![MSH3 antibody [C2C3], C-term detects MSH3 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MSH3 stained by MSH3 antibody [C2C3], C-term (NBP2-19417) diluted at 1:500.Blue: Fluoroshield with DAPI .Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp2-19417_rabbit-polyclonal-msh3-antibody-immunocytochemistry-immunofluorescence-2611202518402224.jpg)
![A549 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MSH3 antibody [C2C3], C-term (NBP2-19417) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19417_rabbit-polyclonal-msh3-antibody-western-blot-2611202518202319.jpg)
![Western Blot: RPGR Antibody [NBP2-93653] - Western blot analysis of extracts of Rat testis, using RPGR antibody (NBP2-93653) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/RPGR-Antibody-Western-Blot-NBP2-93653-img0001.jpg "Western Blot: RPGR Antibody [NBP2-93653] - Western blot analysis of extracts of Rat testis, using RPGR antibody (NBP2-93653) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: RPGR Antibody [NBP2-93653] - Western blot analysis of extracts of Mouse brain, using RPGR antibody (NBP2-93653) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/RPGR Antibody-Western Blot-NBP2-93653-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RP1 Antibody [NBP2-22149] - RP1 antibody was tested in ARPE-19 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/RP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22149-img0001.jpg "Immunocytochemistry/Immunofluorescence: RP1 Antibody [NBP2-22149] - RP1 antibody was tested in ARPE-19 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry: RP1 Antibody [NBP2-22149] - IHC analysis of RP1 in mouse retina.](http://images.novusbio.com/fullsize/RP1-Antibody-Immunohistochemistry-NBP2-22149-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204] - Staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red). Cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/AHNAK-Antibody-EM-09-Immunocytochemistry-Immunofluorescence-NBP2-62204-img0002.jpg "Immunocytochemistry/Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204] - Staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red). Cell nuclei stained with DAPI (blue).")
![Immunohistochemistry-Frozen: AHNAK Antibody (EM-09) [NBP2-62204] - Staining (frozen sections) of murine tongue by anti-AHNAK1 antibody (EM-09; red). Actin filaments were decorated by phalloidin (green), cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/AHNAK-Antibody-EM-09-Immunohistochemistry-Frozen-NBP2-62204-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204] - Staining of AHNAK1 in human primary fibroblasts using anti-AHNAK1 (EM-09; green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/AHNAK-Antibody-EM-09-Immunocytochemistry-Immunofluorescence-NBP2-62204-img0001.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ug per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight (TM) 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block for30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.](http://images.novusbio.com/fullsize/alpha 2-Macroglobulin Antibody-Western Blot-NB600-938-img0010.jpg "Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ug per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight (TM) 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block for30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.")
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Analysis using the Biotin conjugate of NB600-938. Detection of Lane 1: . Lane 2: none. Load: 100 ug per lane. Primary antibody:alpha 2-Macroglobulin biotin conjugated antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Western-Blot-NB600-938-img0006.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Detection of Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Western-Blot-NB600-938-img0007.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 163 kDa, 163 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Western-Blot-NB600-938-img0009.jpg)
