MSH3 Antibody (CL14059) - Azide and BSA Free

Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.

Staining in RH-30 cell line with Anti-MSH3 monoclonal antibody) .

Staining of human cervix, uterine shows moderate nuclear positivity in squamous epithelial cells.

Staining of human colon shows moderate nuclear positivity in glandular and lymphoid cells.

Staining of human kidney shows moderate nuclear positivity in cells in tubules and cells in glomeruli.

Staining of human cervical cancer shows strong nuclear positivity in tumor cells.

Staining of human ovarian cancer (papillary ovarian carcinoma) shows moderate nuclear positivity in tumor cells.

Analysis in human cell line U-251 MG.

Staining of human testis shows no positivity in cells in seminiferous ducts.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL14059
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

MSH3 Antibody (CL14059) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a synthetic peptide of P20585, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MSH3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100, IHC-P, Retrieval method: HIER pH6

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for MSH3 Antibody (CL14059) - Azide and BSA Free

• Divergent upstream protein
• DNA mismatch repair protein Msh3
• DUC1
• DUG
• DUPMGC163306
• hMSH3
• Mismatch repair protein 1
• MRP1MGC163308
• mutS (E. coli) homolog 3
• mutS homolog 3 (E. coli)

Background

The function of MSH3 is unknown. It is probably a DNA-repair protein. Defects in MSH3 are a cause of susceptibility to endometrial cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MSH3